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The map label for this gene is yiaO [H]

Identifier: 78043354

GI number: 78043354

Start: 1159123

End: 1160190

Strand: Direct

Name: yiaO [H]

Synonym: CHY_1301

Alternate gene names: 78043354

Gene position: 1159123-1160190 (Clockwise)

Preceding gene: 78044496

Following gene: 78043624

Centisome position: 48.27

GC content: 35.86

Gene sequence:

>1068_bases
ATGAATTTAAGAAAATTCGGGATTTTACTGCTTGCTCTATTATTTACATTTGGAATAATAACTGGTTGCGGCAAAGGTGG
AACGAAAACTGAAGAAAAGAAAAGTAGTGAAAATAACCAGGGAGTAATTGAAATAAAATTATCCCACAGTTCACCGGCAA
CCAATGACCGTTTAGAAACTGCCTGTCAGGCTTTTAAGAAGTATGTAGAAGAACATACCAATGGAAGAGTTAAAGTCACC
ACTTATCCTGCTAGCGTTTTAGGTGGTGAACGGGAGCAGTTGGAAGGAGTTCAAATGGGTACAATTCAAATGGCAGCTTT
AAGTACTGGACCATTTCCAGGAATCTTTCCAGAAATAATGGTATTTGATATGCCTTATCTCTTCCCTCAAGATACCAGAG
TAGTTTACAAAGTATTAGATGGTCCAGTTGGGCAAGAAATAAGTGATTTATTATTGAAAAAAACCGGAGTACGTAATCTT
GCATTTGGGGAAAATGGATTTAGACATTACACAAATAGCGTTAGACCTATCAAGAAACCGGGCGATATGAAGGGATTAAA
GATTAGAACAATGGAAAACCCTGCTCATATGGCTATTGTGAAAGCATTAGGTGGCAGTCCAACTCCCATGGCGTTTAATG
AAGTTTATTCGGCATTACAAACGAAAACTGTTGATGGCCAGGAAAACCCAATTTCTCTAATTGTTTCAATGAAATTTTAT
GAAGTACAGAAATACTTAACCTTAGATGGTCATATATACAATCCTTACCTGCTTATTATTAACGATAAGTTCTATAATAG
TTTACCCGAAGATATTAGAAAAGTAATTGATGAAGGAGCAAAAGTCTGGCAAAAAGTTGAAAGAGAAGAAAACCAAAAAC
AAATCGAAGCTGGGATGAAAGTATTAAAGAATGCGGGAATGGTTATAACTGAACTTACTCCCGAAGAACTTGAAGCATTT
AAGCAGGCAACAAAAACAGTTTACGATCAGTTTAAATCGCAAATTGGTGAAGAACTCTTAAATAAAGTAATCAAGGCAGT
CGAAGAAGCACAAAAAGAAATACAATAA

Upstream 100 bases:

>100_bases
ATAGGGGGTGAAAGTAAAAAAGTTTGATGGTAAATTTTTTAAAAAAATTTTTAGAAGACAATTTGGCTAAAAAAATTAAT
ACTAAAGGAGGAGTGGGAAA

Downstream 100 bases:

>100_bases
GAAAAAATTAAGTAGAAGAGAGCAGTGGCCCCAATTTATAGAAGGGCCAAATGCTCTCTTCAAAATGATAAGTAATGATG
TAAATAGGTAAAAAATAATA

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MNLRKFGILLLALLFTFGIITGCGKGGTKTEEKKSSENNQGVIEIKLSHSSPATNDRLETACQAFKKYVEEHTNGRVKVT
TYPASVLGGEREQLEGVQMGTIQMAALSTGPFPGIFPEIMVFDMPYLFPQDTRVVYKVLDGPVGQEISDLLLKKTGVRNL
AFGENGFRHYTNSVRPIKKPGDMKGLKIRTMENPAHMAIVKALGGSPTPMAFNEVYSALQTKTVDGQENPISLIVSMKFY
EVQKYLTLDGHIYNPYLLIINDKFYNSLPEDIRKVIDEGAKVWQKVEREENQKQIEAGMKVLKNAGMVITELTPEELEAF
KQATKTVYDQFKSQIGEELLNKVIKAVEEAQKEIQ

Sequences:

>Translated_355_residues
MNLRKFGILLLALLFTFGIITGCGKGGTKTEEKKSSENNQGVIEIKLSHSSPATNDRLETACQAFKKYVEEHTNGRVKVT
TYPASVLGGEREQLEGVQMGTIQMAALSTGPFPGIFPEIMVFDMPYLFPQDTRVVYKVLDGPVGQEISDLLLKKTGVRNL
AFGENGFRHYTNSVRPIKKPGDMKGLKIRTMENPAHMAIVKALGGSPTPMAFNEVYSALQTKTVDGQENPISLIVSMKFY
EVQKYLTLDGHIYNPYLLIINDKFYNSLPEDIRKVIDEGAKVWQKVEREENQKQIEAGMKVLKNAGMVITELTPEELEAF
KQATKTVYDQFKSQIGEELLNKVIKAVEEAQKEIQ
>Mature_355_residues
MNLRKFGILLLALLFTFGIITGCGKGGTKTEEKKSSENNQGVIEIKLSHSSPATNDRLETACQAFKKYVEEHTNGRVKVT
TYPASVLGGEREQLEGVQMGTIQMAALSTGPFPGIFPEIMVFDMPYLFPQDTRVVYKVLDGPVGQEISDLLLKKTGVRNL
AFGENGFRHYTNSVRPIKKPGDMKGLKIRTMENPAHMAIVKALGGSPTPMAFNEVYSALQTKTVDGQENPISLIVSMKFY
EVQKYLTLDGHIYNPYLLIINDKFYNSLPEDIRKVIDEGAKVWQKVEREENQKQIEAGMKVLKNAGMVITELTPEELEAF
KQATKTVYDQFKSQIGEELLNKVIKAVEEAQKEIQ

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=303, Percent_Identity=38.9438943894389, Blast_Score=197, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 39703; Mature: 39703

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRKFGILLLALLFTFGIITGCGKGGTKTEEKKSSENNQGVIEIKLSHSSPATNDRLET
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHH
ACQAFKKYVEEHTNGRVKVTTYPASVLGGEREQLEGVQMGTIQMAALSTGPFPGIFPEIM
HHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHHHCCCEECCEEEEEECCCCCCCCCHHHH
VFDMPYLFPQDTRVVYKVLDGPVGQEISDLLLKKTGVRNLAFGENGFRHYTNSVRPIKKP
HHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCC
GDMKGLKIRTMENPAHMAIVKALGGSPTPMAFNEVYSALQTKTVDGQENPISLIVSMKFY
CCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHH
EVQKYLTLDGHIYNPYLLIINDKFYNSLPEDIRKVIDEGAKVWQKVEREENQKQIEAGMK
HHHHHHEECCEEECCEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKNAGMVITELTPEELEAFKQATKTVYDQFKSQIGEELLNKVIKAVEEAQKEIQ
HHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLRKFGILLLALLFTFGIITGCGKGGTKTEEKKSSENNQGVIEIKLSHSSPATNDRLET
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHH
ACQAFKKYVEEHTNGRVKVTTYPASVLGGEREQLEGVQMGTIQMAALSTGPFPGIFPEIM
HHHHHHHHHHHHCCCEEEEEECCHHHHCCCHHHHCCCEECCEEEEEECCCCCCCCCHHHH
VFDMPYLFPQDTRVVYKVLDGPVGQEISDLLLKKTGVRNLAFGENGFRHYTNSVRPIKKP
HHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCC
GDMKGLKIRTMENPAHMAIVKALGGSPTPMAFNEVYSALQTKTVDGQENPISLIVSMKFY
CCCCCEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHH
EVQKYLTLDGHIYNPYLLIINDKFYNSLPEDIRKVIDEGAKVWQKVEREENQKQIEAGMK
HHHHHHEECCEEECCEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKNAGMVITELTPEELEAFKQATKTVYDQFKSQIGEELLNKVIKAVEEAQKEIQ
HHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]