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The map label for this gene is aroKB [H]

Identifier: 78042786

GI number: 78042786

Start: 542719

End: 544308

Strand: Direct

Name: aroKB [H]

Synonym: CHY_0629

Alternate gene names: 78042786

Gene position: 542719-544308 (Clockwise)

Preceding gene: 78042963

Following gene: 78044711

Centisome position: 22.6

GC content: 40.94

Gene sequence:

>1590_bases
ATGAACATCATTTTAGTGGGACTACCGGGGGCTGGCAAAACACATATAGGTCGGATTTTAGCGAGGAAATTAAAAAGGAA
TTTTGTGGATCTTGACCGGAAGATTGAAGAAGAGACGGGACTTACGATTAAAGAAATATTTGCCCGTTTCGGTGAGCGCT
ATTTCCGCCGTTTGGAAGTAAAGAAACTACAGGAACTTTTTAAACTTCATAATGCGGTGATTGCCACCGGTGGGGGAACG
GTAGAACGAATGGATGCGAGAAGAAAGTTAAAAAATCTTGGCTTAGTGGTTTATTTAAAGGCGTCCCCGGAAGTTATAAT
CCGGCGTCTTATTAATTTAGAAAATAGACCAATACTTACCCAGGGGGACCCTTTAGAAAAGCTTTCGGAGCTTTACCGTC
GCCGGGATAAGTTTTACCGGGAAGTTTCCGATGTAGCAATTAATACCGAAGGTAAAGACTCAGGCGAGGTCGTTGACGCG
ATCCTAAAATCCCTGACTATTGATGGTTTACGGTTTCCCCGGAAACTTTACATTAATTTGGCTGAAAATAGTTATCCGGT
ATATTTTGGCGAAAAAGTGGCGTTAAAAGCGGGTGAGTTTTTAAAAGAAAAAGTTACCGGGAAAAAGATTTTAATTGTTT
CCCAGGAAAATATCTTTGGTTTATATGGCAAAACTTTAGAAGAAAGTTTAACAAAGGAAGGTTTTAAAACCGACGTTTTT
TTTCTTCCCCAGGGGGAAGTTGCAAAATCTATGGAATATATTGTGAAGCTTTACGATAAAGCCATAGAGTTTGGACTACG
CCGCCATGATACCGTTATGGCCTTTGGGGGAGGAGTAGTAGGAGATGCTACTGGATTTTTTGCCGCAACCTATTTGCGGG
GAATCAATTTTGTCCAGGTACCGACAACTCTTCTTTCTATGGTGGATAGCAGTGTAGGGGGGAAAGTGGGGATTAACCTT
CCCCAAGGGAAAAACCTGGTAGGAGCCTTTTACCAGCCAAAAGTAGTGTTAATAGATACTACTTACCTAAAAACTCTTCC
CAGAAGAGAAGTGCTGGCGGGATTGGCTGAAATTTATAAAGCGTTTTTAATTGCTGAAGAAAGCCTTGTCGGGTGGCTTT
CCGGATTAAACCTGGATAAGCTCCGTCCAAGGGATTGGCAAAGGATTATCAAGGCGGCGGTAAAAATAAAAGCAAAGGTT
GTATCCCTCGATGAACGGGAAGAAGGGTTACGGGCGGTATTAAATTTAGGCCACACCATAGGCCATGCGATAGAAGGTGT
AAACGGGTTTTCCGGTATTCTTCACGGCGAAGCGGTAGCTCTGGGAATATTATGGGAAGCCAAGCTCTCTAAAGATTTAG
GATATTTAAGGGAGGAGGAATGGGAAAAAATACGGGAGTTTATCGAGAAAAATTATGCTTACTTAAAACTATCCTTAAAT
CCCGATAGCTTACTTGTTTTTATGGATAAAGATAAAAAAAATCGGGAAGATATCGCTTTTATGCTTTTAAAGAAGATTGG
TGAATACCCGGAAAATGTTGAAATAAAGAGAGAATGGTTACAAAAGTGGTTAACGTTGAATTTTTCTTAA

Upstream 100 bases:

>100_bases
ATGTTGCTGTGAAGGCGGTAATTGGCAGGATTGGGGGAGCAACTTCCGGCTTTTTTTGTATATTTTTATTTGTATATTTT
TAAAGGAAAGGGTTGTTAAA

Downstream 100 bases:

>100_bases
AGGATTTTTGTTTTTTTTTGACGAACTCTTAAGGTAAAATCCGATGGGAGTGCGGAGCTAAGATGAGTGAAGCGAAAGAT
GGCAAAAAAACCAAGAGACT

Product: shikimate kinase/3-dehydroquinate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MNIILVGLPGAGKTHIGRILARKLKRNFVDLDRKIEEETGLTIKEIFARFGERYFRRLEVKKLQELFKLHNAVIATGGGT
VERMDARRKLKNLGLVVYLKASPEVIIRRLINLENRPILTQGDPLEKLSELYRRRDKFYREVSDVAINTEGKDSGEVVDA
ILKSLTIDGLRFPRKLYINLAENSYPVYFGEKVALKAGEFLKEKVTGKKILIVSQENIFGLYGKTLEESLTKEGFKTDVF
FLPQGEVAKSMEYIVKLYDKAIEFGLRRHDTVMAFGGGVVGDATGFFAATYLRGINFVQVPTTLLSMVDSSVGGKVGINL
PQGKNLVGAFYQPKVVLIDTTYLKTLPRREVLAGLAEIYKAFLIAEESLVGWLSGLNLDKLRPRDWQRIIKAAVKIKAKV
VSLDEREEGLRAVLNLGHTIGHAIEGVNGFSGILHGEAVALGILWEAKLSKDLGYLREEEWEKIREFIEKNYAYLKLSLN
PDSLLVFMDKDKKNREDIAFMLLKKIGEYPENVEIKREWLQKWLTLNFS

Sequences:

>Translated_529_residues
MNIILVGLPGAGKTHIGRILARKLKRNFVDLDRKIEEETGLTIKEIFARFGERYFRRLEVKKLQELFKLHNAVIATGGGT
VERMDARRKLKNLGLVVYLKASPEVIIRRLINLENRPILTQGDPLEKLSELYRRRDKFYREVSDVAINTEGKDSGEVVDA
ILKSLTIDGLRFPRKLYINLAENSYPVYFGEKVALKAGEFLKEKVTGKKILIVSQENIFGLYGKTLEESLTKEGFKTDVF
FLPQGEVAKSMEYIVKLYDKAIEFGLRRHDTVMAFGGGVVGDATGFFAATYLRGINFVQVPTTLLSMVDSSVGGKVGINL
PQGKNLVGAFYQPKVVLIDTTYLKTLPRREVLAGLAEIYKAFLIAEESLVGWLSGLNLDKLRPRDWQRIIKAAVKIKAKV
VSLDEREEGLRAVLNLGHTIGHAIEGVNGFSGILHGEAVALGILWEAKLSKDLGYLREEEWEKIREFIEKNYAYLKLSLN
PDSLLVFMDKDKKNREDIAFMLLKKIGEYPENVEIKREWLQKWLTLNFS
>Mature_529_residues
MNIILVGLPGAGKTHIGRILARKLKRNFVDLDRKIEEETGLTIKEIFARFGERYFRRLEVKKLQELFKLHNAVIATGGGT
VERMDARRKLKNLGLVVYLKASPEVIIRRLINLENRPILTQGDPLEKLSELYRRRDKFYREVSDVAINTEGKDSGEVVDA
ILKSLTIDGLRFPRKLYINLAENSYPVYFGEKVALKAGEFLKEKVTGKKILIVSQENIFGLYGKTLEESLTKEGFKTDVF
FLPQGEVAKSMEYIVKLYDKAIEFGLRRHDTVMAFGGGVVGDATGFFAATYLRGINFVQVPTTLLSMVDSSVGGKVGINL
PQGKNLVGAFYQPKVVLIDTTYLKTLPRREVLAGLAEIYKAFLIAEESLVGWLSGLNLDKLRPRDWQRIIKAAVKIKAKV
VSLDEREEGLRAVLNLGHTIGHAIEGVNGFSGILHGEAVALGILWEAKLSKDLGYLREEEWEKIREFIEKNYAYLKLSLN
PDSLLVFMDKDKKNREDIAFMLLKKIGEYPENVEIKREWLQKWLTLNFS

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; second step. [C]

COG id: COG0337

COG function: function code E; 3-dehydroquinate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dehydroquinate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789791, Length=326, Percent_Identity=41.7177914110429, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082255, Length=165, Percent_Identity=38.7878787878788, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1786587, Length=167, Percent_Identity=37.125748502994, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320332, Length=279, Percent_Identity=37.9928315412186, Blast_Score=179, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016303
- InterPro:   IPR002658
- InterPro:   IPR016037 [H]

Pfam domain/function: PF01761 DHQ_synthase [H]

EC number: =4.2.3.4 [H]

Molecular weight: Translated: 59960; Mature: 59960

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIILVGLPGAGKTHIGRILARKLKRNFVDLDRKIEEETGLTIKEIFARFGERYFRRLEV
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KKLQELFKLHNAVIATGGGTVERMDARRKLKNLGLVVYLKASPEVIIRRLINLENRPILT
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCEEC
QGDPLEKLSELYRRRDKFYREVSDVAINTEGKDSGEVVDAILKSLTIDGLRFPRKLYINL
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
AENSYPVYFGEKVALKAGEFLKEKVTGKKILIVSQENIFGLYGKTLEESLTKEGFKTDVF
CCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
FLPQGEVAKSMEYIVKLYDKAIEFGLRRHDTVMAFGGGVVGDATGFFAATYLRGINFVQV
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEHH
PTTLLSMVDSSVGGKVGINLPQGKNLVGAFYQPKVVLIDTTYLKTLPRREVLAGLAEIYK
HHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCEEEEEECHHHHHCCHHHHHHHHHHHHH
AFLIAEESLVGWLSGLNLDKLRPRDWQRIIKAAVKIKAKVVSLDEREEGLRAVLNLGHTI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHH
GHAIEGVNGFSGILHGEAVALGILWEAKLSKDLGYLREEEWEKIREFIEKNYAYLKLSLN
HHHHCCCCCHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
PDSLLVFMDKDKKNREDIAFMLLKKIGEYPENVEIKREWLQKWLTLNFS
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNIILVGLPGAGKTHIGRILARKLKRNFVDLDRKIEEETGLTIKEIFARFGERYFRRLEV
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KKLQELFKLHNAVIATGGGTVERMDARRKLKNLGLVVYLKASPEVIIRRLINLENRPILT
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCEEC
QGDPLEKLSELYRRRDKFYREVSDVAINTEGKDSGEVVDAILKSLTIDGLRFPRKLYINL
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
AENSYPVYFGEKVALKAGEFLKEKVTGKKILIVSQENIFGLYGKTLEESLTKEGFKTDVF
CCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEE
FLPQGEVAKSMEYIVKLYDKAIEFGLRRHDTVMAFGGGVVGDATGFFAATYLRGINFVQV
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEHH
PTTLLSMVDSSVGGKVGINLPQGKNLVGAFYQPKVVLIDTTYLKTLPRREVLAGLAEIYK
HHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCEEEEEECHHHHHCCHHHHHHHHHHHHH
AFLIAEESLVGWLSGLNLDKLRPRDWQRIIKAAVKIKAKVVSLDEREEGLRAVLNLGHTI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHH
GHAIEGVNGFSGILHGEAVALGILWEAKLSKDLGYLREEEWEKIREFIEKNYAYLKLSLN
HHHHCCCCCHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
PDSLLVFMDKDKKNREDIAFMLLKKIGEYPENVEIKREWLQKWLTLNFS
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA