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The map label for this gene is pilB [H]

Identifier: 78044711

GI number: 78044711

Start: 544371

End: 546077

Strand: Direct

Name: pilB [H]

Synonym: CHY_0630

Alternate gene names: 78044711

Gene position: 544371-546077 (Clockwise)

Preceding gene: 78042786

Following gene: 78044094

Centisome position: 22.67

GC content: 45.23

Gene sequence:

>1707_bases
ATGAGTGAAGCGAAAGATGGCAAAAAAACCAAGAGACTGGGTGATTTTTTAGTAGAAAATGGGCTTATTACCGAAGAACA
ATTACAGAAAGCTTTGGAGTATCAGAAAAGAACGGGAGAGCGCCTGGGACAAGCTTTGATTAAACTTGGCTATGTTTCGG
AACAGGATATCATGGGGGTTTTAGAGTTTCAATTGGGAATTCCCCAGGTTGCTTTATATAATTATTCGTTAGACCCGGCC
TTAATTAAAAGCATACCCGAGTCGCTAATTAAAAGGCATAAGGTAATACCCTTAAAACGCGAGGGCAACCGCTTAACCGT
TGCCATGGTTGACCCTTTAAATGTGGTTGCCATTGACGACTTAAGGCTTGCCACCAATTTAGATATTATCCCGGTCATTG
CTTCGGAAAAAGAAATTGATGCCGTTATTAACCGTTTTTTTGGCGCTATTGATTTAAACGAAACCATTAAGGACATAGAA
AAAGCGGGCGAAGAGCCGGAAGTAATAAAAAATGAAGAGACAGAAGAGGTTTTGGTTGATGAAGCGCCGGTGGTGCGACT
GGTGAATTCCCTCTTACTGCAGGCGGTAAACCAGAGGGCGTCCGATGTCCATATTGAGCCTTTTGAAGATATGGTAAGAG
TACGCTTTAGGGTAGACGGTTTTTTAAGAGAAGTTATGACTTTGCCCCGGGCGATTAGAAGTTCCCTTACTTCCCGGATA
AAAATAATGGCCAATATGAACATTACCGAGAAGCGGCTTCCCCAGGATGGGCGGATTAAGATAAAAGTAGCCAATCGCCA
GGTAGATATGCGGGTGGCTACAACTCCGACGTTATTTGGGGAAAAGGTGGTCATAAGACTTCTGGATCAGGAAAATGCCT
TTTTAACTTTAGATAGACTGGGACTTTCCAGTAAAAACAGCGAAAGATTTAATAAAATCCTGGCCCAACCCCACGGCATG
ATTTTAATTGCCGGCCCTACCGGAAGCGGCAAAACTACCACCCTTTATGCTGCTTTAAACCAAATTCATGACCCTTCCAA
AAATATTATTACCATTGAGGACCCAGTTGAGTATGTTATGCCCGGGGTAACCCAAATCCAGGTTAATCCGAAAGTTGGAC
TCACCTTTGCGGTGGGCTTGCGCTCAATTCTGCGCCTTGATCCCGATATCATTATGGTAGGCGAAATTAGGGACCGGGAA
ACTGCAGAAATAGCGGTAAAAGCGGCCAATACCGGGCATCTTGTATTATCTACCATTCACACCAATGATGCAGTTTCTAC
TGTGAGCCGTCTGGTAGAAATGGGGATTGAAGCGTACTTGGTTGCTGCTTCGCTTGTGGGAATAGTGGCTCAACGCCTGG
TGCGCAAACTTTGCCCGGAATGCAAAGCTTCTACTAAAATTGGTCCCGATGATCCTGCTTATCATGCCTTAAATCTCGGC
CAAGGAGAGACGGTAACCGTTTACCGGCCGGTGGGCTGCCCGGCATGTGAAAACACGGGCTATAAAGGGCGGGTTGGCAT
CCATGAAGTTTTAATAAATTCTCGAAAAGTTAGAAATCTTATTGTCAACGGCGGCTCTTATGATGAAATGTTGGCAACCG
TGCGGGAAGAAGGAATGGTCTTAATGTTGGAAGACGGGCGGGAAAAAGTTCTGGCAGGTATTACCGCGCCGGAAGAGATT
ATCCGGGTGGCCAGTACGGTTAACTAA

Upstream 100 bases:

>100_bases
AATGGTTACAAAAGTGGTTAACGTTGAATTTTTCTTAAAGGATTTTTGTTTTTTTTTGACGAACTCTTAAGGTAAAATCC
GATGGGAGTGCGGAGCTAAG

Downstream 100 bases:

>100_bases
AAGGTGGCAGGTGAAGTTTTTATGATTAATGAGCTTTTACGGTTAATGGTAGAGTTTAAAGCGTCGGACTTGCATTTAAC
GGTGAACTTTCCCCCGGCTT

Product: type IV pilus assembly protein PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSEAKDGKKTKRLGDFLVENGLITEEQLQKALEYQKRTGERLGQALIKLGYVSEQDIMGVLEFQLGIPQVALYNYSLDPA
LIKSIPESLIKRHKVIPLKREGNRLTVAMVDPLNVVAIDDLRLATNLDIIPVIASEKEIDAVINRFFGAIDLNETIKDIE
KAGEEPEVIKNEETEEVLVDEAPVVRLVNSLLLQAVNQRASDVHIEPFEDMVRVRFRVDGFLREVMTLPRAIRSSLTSRI
KIMANMNITEKRLPQDGRIKIKVANRQVDMRVATTPTLFGEKVVIRLLDQENAFLTLDRLGLSSKNSERFNKILAQPHGM
ILIAGPTGSGKTTTLYAALNQIHDPSKNIITIEDPVEYVMPGVTQIQVNPKVGLTFAVGLRSILRLDPDIIMVGEIRDRE
TAEIAVKAANTGHLVLSTIHTNDAVSTVSRLVEMGIEAYLVAASLVGIVAQRLVRKLCPECKASTKIGPDDPAYHALNLG
QGETVTVYRPVGCPACENTGYKGRVGIHEVLINSRKVRNLIVNGGSYDEMLATVREEGMVLMLEDGREKVLAGITAPEEI
IRVASTVN

Sequences:

>Translated_568_residues
MSEAKDGKKTKRLGDFLVENGLITEEQLQKALEYQKRTGERLGQALIKLGYVSEQDIMGVLEFQLGIPQVALYNYSLDPA
LIKSIPESLIKRHKVIPLKREGNRLTVAMVDPLNVVAIDDLRLATNLDIIPVIASEKEIDAVINRFFGAIDLNETIKDIE
KAGEEPEVIKNEETEEVLVDEAPVVRLVNSLLLQAVNQRASDVHIEPFEDMVRVRFRVDGFLREVMTLPRAIRSSLTSRI
KIMANMNITEKRLPQDGRIKIKVANRQVDMRVATTPTLFGEKVVIRLLDQENAFLTLDRLGLSSKNSERFNKILAQPHGM
ILIAGPTGSGKTTTLYAALNQIHDPSKNIITIEDPVEYVMPGVTQIQVNPKVGLTFAVGLRSILRLDPDIIMVGEIRDRE
TAEIAVKAANTGHLVLSTIHTNDAVSTVSRLVEMGIEAYLVAASLVGIVAQRLVRKLCPECKASTKIGPDDPAYHALNLG
QGETVTVYRPVGCPACENTGYKGRVGIHEVLINSRKVRNLIVNGGSYDEMLATVREEGMVLMLEDGREKVLAGITAPEEI
IRVASTVN
>Mature_567_residues
SEAKDGKKTKRLGDFLVENGLITEEQLQKALEYQKRTGERLGQALIKLGYVSEQDIMGVLEFQLGIPQVALYNYSLDPAL
IKSIPESLIKRHKVIPLKREGNRLTVAMVDPLNVVAIDDLRLATNLDIIPVIASEKEIDAVINRFFGAIDLNETIKDIEK
AGEEPEVIKNEETEEVLVDEAPVVRLVNSLLLQAVNQRASDVHIEPFEDMVRVRFRVDGFLREVMTLPRAIRSSLTSRIK
IMANMNITEKRLPQDGRIKIKVANRQVDMRVATTPTLFGEKVVIRLLDQENAFLTLDRLGLSSKNSERFNKILAQPHGMI
LIAGPTGSGKTTTLYAALNQIHDPSKNIITIEDPVEYVMPGVTQIQVNPKVGLTFAVGLRSILRLDPDIIMVGEIRDRET
AEIAVKAANTGHLVLSTIHTNDAVSTVSRLVEMGIEAYLVAASLVGIVAQRLVRKLCPECKASTKIGPDDPAYHALNLGQ
GETVTVYRPVGCPACENTGYKGRVGIHEVLINSRKVRNLIVNGGSYDEMLATVREEGMVLMLEDGREKVLAGITAPEEII
RVASTVN

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=440, Percent_Identity=43.8636363636364, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1786296, Length=432, Percent_Identity=40.2777777777778, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI87082188, Length=250, Percent_Identity=34.8, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62721; Mature: 62590

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKDGKKTKRLGDFLVENGLITEEQLQKALEYQKRTGERLGQALIKLGYVSEQDIMGV
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LEFQLGIPQVALYNYSLDPALIKSIPESLIKRHKVIPLKREGNRLTVAMVDPLNVVAIDD
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCEEEEEEC
LRLATNLDIIPVIASEKEIDAVINRFFGAIDLNETIKDIEKAGEEPEVIKNEETEEVLVD
EEEECCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCHHHHHHC
EAPVVRLVNSLLLQAVNQRASDVHIEPFEDMVRVRFRVDGFLREVMTLPRAIRSSLTSRI
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KIMANMNITEKRLPQDGRIKIKVANRQVDMRVATTPTLFGEKVVIRLLDQENAFLTLDRL
EEEECCCCHHHHCCCCCEEEEEEECCEEEEEEECCCCCCCCHHHHEEECCCCCEEEEECC
GLSSKNSERFNKILAQPHGMILIAGPTGSGKTTTLYAALNQIHDPSKNIITIEDPVEYVM
CCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHCCCCCCEEEEECCHHHHC
PGVTQIQVNPKVGLTFAVGLRSILRLDPDIIMVGEIRDRETAEIAVKAANTGHLVLSTIH
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEEE
TNDAVSTVSRLVEMGIEAYLVAASLVGIVAQRLVRKLCPECKASTKIGPDDPAYHALNLG
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEEECC
QGETVTVYRPVGCPACENTGYKGRVGIHEVLINSRKVRNLIVNGGSYDEMLATVREEGMV
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHEEEECCCCHHHHHHHHHHCCEE
LMLEDGREKVLAGITAPEEIIRVASTVN
EEEECCCCEEEECCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SEAKDGKKTKRLGDFLVENGLITEEQLQKALEYQKRTGERLGQALIKLGYVSEQDIMGV
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LEFQLGIPQVALYNYSLDPALIKSIPESLIKRHKVIPLKREGNRLTVAMVDPLNVVAIDD
HHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCEEEEEEC
LRLATNLDIIPVIASEKEIDAVINRFFGAIDLNETIKDIEKAGEEPEVIKNEETEEVLVD
EEEECCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCCCHHHHHHC
EAPVVRLVNSLLLQAVNQRASDVHIEPFEDMVRVRFRVDGFLREVMTLPRAIRSSLTSRI
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
KIMANMNITEKRLPQDGRIKIKVANRQVDMRVATTPTLFGEKVVIRLLDQENAFLTLDRL
EEEECCCCHHHHCCCCCEEEEEEECCEEEEEEECCCCCCCCHHHHEEECCCCCEEEEECC
GLSSKNSERFNKILAQPHGMILIAGPTGSGKTTTLYAALNQIHDPSKNIITIEDPVEYVM
CCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHCCCCCCEEEEECCHHHHC
PGVTQIQVNPKVGLTFAVGLRSILRLDPDIIMVGEIRDRETAEIAVKAANTGHLVLSTIH
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEEE
TNDAVSTVSRLVEMGIEAYLVAASLVGIVAQRLVRKLCPECKASTKIGPDDPAYHALNLG
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEEECC
QGETVTVYRPVGCPACENTGYKGRVGIHEVLINSRKVRNLIVNGGSYDEMLATVREEGMV
CCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCHHHHEEEECCCCHHHHHHHHHHCCEE
LMLEDGREKVLAGITAPEEIIRVASTVN
EEEECCCCEEEECCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]