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The map label for this gene is cysW [H]

Identifier: 77465707

GI number: 77465707

Start: 820139

End: 820978

Strand: Direct

Name: cysW [H]

Synonym: RSP_3699

Alternate gene names: 77465707

Gene position: 820139-820978 (Clockwise)

Preceding gene: 77465706

Following gene: 77465709

Centisome position: 86.97

GC content: 69.17

Gene sequence:

>840_bases
ATGAGCACGGCCGCCCTCACCGCCCCCGGCGCGCTCGCCCCACGGGCAAGCCGGCTCGGGCCGCGCCTTCTGATCGGGGC
CGCGCTGCTTCTGACCCTCTTCATGGTCGTGGTGCCGATGGCCTTCATCTTTCACCGCGCCTTCGCCGAGGGCTGGCGTC
TCTTTGCCCAGAACATCCTCGCGCCCGACACGCTGCATGCGATCGGGCTCACGACGCTGGTCGCCGTCATCGTGGTGCCG
GTGAACGTGGCCTTCGGCATCGCCGCCGCCTGGGCCATCGCGCGTTTCCGCTTCATCGGGCGCAAGACGCTGCTCACGCT
GATCGAGATCCCCTTCTCGATCTCGCCCATCATCGCGGGCATCTGCTACCTGCTGCTCTACGGCCGTCAGGGGCTTCTGG
GCCCCACGCTGCTGTCGCACGACATCCAGATCCTCTTCGCGCTGCCCGGCATCGTGCTGGTCACGCTCTTCGTGACCTCG
CCCTTCGTCGTGCGCGAGGTGCTGCCCCTGATGCAGGCCCAGGGGTCCGAGCAGGAGCAGGCGGCGCTGACCCTCGGCGC
CTCGGGCTGGCAGGTGTTCCGCCGGGTGACGCTGCCGAACATCCGCTGGGCGCTTCTCTACGGCGCGATCCTCTGCACCG
CGCGGGCGGTGGGCGAATATGGCGGGGTGTCGGTCGTCTCGGGCAACGTGCGCGGCCAGACCAACACGCTGCCGCTGCAG
ATCGACCTCCTCTTCAACGACCTGAACGTGGCGGGCGCCTTCGCCGCGGCCACCACCCTCACGCTTCTGGCGCTGGTGAT
CCTCGTCCTGAAGACGGTGATGGAGAGCCGGCGCGGCTGA

Upstream 100 bases:

>100_bases
CGGCGGTGATCGCCCTCACGCTCCTGGCCATCGCGCTGGTGCTGCTCGGGGCCTCGAACCTGCTGCAGGCGCGGGCCCTG
CGCTACAAGGGGAAACAGGC

Downstream 100 bases:

>100_bases
GGCCTCGGGACAGCCGGCAACTGCCCTGCCCCCTGACGCGGAGGGCGCGTGGATCGGTCACTCGGCCCGGAGCAAGGGCA
GCCCGGCGGTCAGAGGTGGC

Product: ABC sulfate/thiosulfate transporter, inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSTAALTAPGALAPRASRLGPRLLIGAALLLTLFMVVVPMAFIFHRAFAEGWRLFAQNILAPDTLHAIGLTTLVAVIVVP
VNVAFGIAAAWAIARFRFIGRKTLLTLIEIPFSISPIIAGICYLLLYGRQGLLGPTLLSHDIQILFALPGIVLVTLFVTS
PFVVREVLPLMQAQGSEQEQAALTLGASGWQVFRRVTLPNIRWALLYGAILCTARAVGEYGGVSVVSGNVRGQTNTLPLQ
IDLLFNDLNVAGAFAAATTLTLLALVILVLKTVMESRRG

Sequences:

>Translated_279_residues
MSTAALTAPGALAPRASRLGPRLLIGAALLLTLFMVVVPMAFIFHRAFAEGWRLFAQNILAPDTLHAIGLTTLVAVIVVP
VNVAFGIAAAWAIARFRFIGRKTLLTLIEIPFSISPIIAGICYLLLYGRQGLLGPTLLSHDIQILFALPGIVLVTLFVTS
PFVVREVLPLMQAQGSEQEQAALTLGASGWQVFRRVTLPNIRWALLYGAILCTARAVGEYGGVSVVSGNVRGQTNTLPLQ
IDLLFNDLNVAGAFAAATTLTLLALVILVLKTVMESRRG
>Mature_278_residues
STAALTAPGALAPRASRLGPRLLIGAALLLTLFMVVVPMAFIFHRAFAEGWRLFAQNILAPDTLHAIGLTTLVAVIVVPV
NVAFGIAAAWAIARFRFIGRKTLLTLIEIPFSISPIIAGICYLLLYGRQGLLGPTLLSHDIQILFALPGIVLVTLFVTSP
FVVREVLPLMQAQGSEQEQAALTLGASGWQVFRRVTLPNIRWALLYGAILCTARAVGEYGGVSVVSGNVRGQTNTLPLQI
DLLFNDLNVAGAFAAATTLTLLALVILVLKTVMESRRG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=263, Percent_Identity=55.893536121673, Blast_Score=305, Evalue=1e-84,
Organism=Escherichia coli, GI1788764, Length=229, Percent_Identity=33.1877729257642, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786980, Length=210, Percent_Identity=29.0476190476191, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29807; Mature: 29676

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAALTAPGALAPRASRLGPRLLIGAALLLTLFMVVVPMAFIFHRAFAEGWRLFAQNIL
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APDTLHAIGLTTLVAVIVVPVNVAFGIAAAWAIARFRFIGRKTLLTLIEIPFSISPIIAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ICYLLLYGRQGLLGPTLLSHDIQILFALPGIVLVTLFVTSPFVVREVLPLMQAQGSEQEQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHH
AALTLGASGWQVFRRVTLPNIRWALLYGAILCTARAVGEYGGVSVVSGNVRGQTNTLPLQ
HHHEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEE
IDLLFNDLNVAGAFAAATTLTLLALVILVLKTVMESRRG
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STAALTAPGALAPRASRLGPRLLIGAALLLTLFMVVVPMAFIFHRAFAEGWRLFAQNIL
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APDTLHAIGLTTLVAVIVVPVNVAFGIAAAWAIARFRFIGRKTLLTLIEIPFSISPIIAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ICYLLLYGRQGLLGPTLLSHDIQILFALPGIVLVTLFVTSPFVVREVLPLMQAQGSEQEQ
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHH
AALTLGASGWQVFRRVTLPNIRWALLYGAILCTARAVGEYGGVSVVSGNVRGQTNTLPLQ
HHHEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEE
IDLLFNDLNVAGAFAAATTLTLLALVILVLKTVMESRRG
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]