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The map label for this gene is cysT [H]

Identifier: 77465706

GI number: 77465706

Start: 819285

End: 820142

Strand: Direct

Name: cysT [H]

Synonym: RSP_3698

Alternate gene names: 77465706

Gene position: 819285-820142 (Clockwise)

Preceding gene: 77465705

Following gene: 77465707

Centisome position: 86.88

GC content: 69.46

Gene sequence:

>858_bases
ATGATCGCGCGCACCGCACCCGGCCCGGCCCGCCGGTCGAAACGGGTCCTGCCCGGCTTCGGCCTGACCATGGGCATGGT
GCTGACCTATCTGGGCGTCATCGTGCTGCTGCCCGTGGTGGCGCTGGTGCTGAAGGGGGCCGACATCGGCCCCGCCCGCT
TCTGGGCCATCGTCACCGCGCCGCGAACGCTCGCCGCGCTGCGGCTGACGCTGCTGGCGGCGGGCATCGCCACCCTGGTC
AACACGCTCTACGGGCTTCTCATGGCCTGGGTGCTGGTGCGGTACGAGTTTCCGGGCAAGCGGTTCCTCGATGCGATGGT
GGACATCCCCTTCGCTCTGCCGACCGCGGTCGCCGGCCTGTCGCTCTCGGCGCTTTTCGCCGCGAGCGGCTGGTATGGCG
CGATCCTCGAACCCATGGGCATTCAGGTGGTCTACACGATCTGGGGCGTGGCCGCGGCCATGTCCTTCACCTCGATCCCC
TTCGTGATCCGCACCGTCCAGCCGGTGCTCGAGGATCTCGACCCGCAGTTCGAGCAGGCGGCGGTGACGCTGGGCGCCCA
CCCCTTCACCATCTTCCGCCGGGTGATCCTGCCCGCCATCGCCCCCGCGCTGCTGGTGGGGGTCACCCTCTCCTTCGCCC
GCTCGCTGGGCGAGTTCGGCGCCGTGGTCTTCATCGCGGGCAACCTGCCCATGAAGACCGAGATCGCCTCGCTGCTCGCG
GTGATCCGGCTCGAGGAATACGATTACAACGGCGCGGCGGTGATCGCCCTCACGCTCCTGGCCATCGCGCTGGTGCTGCT
CGGGGCCTCGAACCTGCTGCAGGCGCGGGCCCTGCGCTACAAGGGGAAACAGGCATGA

Upstream 100 bases:

>100_bases
TGAAGCTCGTCGATGTCGACGAGACCTTCGGCGGCTGGGAGAAGATCAAGGCCGAGCATTTCGCCGAGGGCGGCATCCTC
GACACGGTCTTCGTGAACCA

Downstream 100 bases:

>100_bases
GCACGGCCGCCCTCACCGCCCCCGGCGCGCTCGCCCCACGGGCAAGCCGGCTCGGGCCGCGCCTTCTGATCGGGGCCGCG
CTGCTTCTGACCCTCTTCAT

Product: ABC sulfate/thiosulfate transporter, inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MIARTAPGPARRSKRVLPGFGLTMGMVLTYLGVIVLLPVVALVLKGADIGPARFWAIVTAPRTLAALRLTLLAAGIATLV
NTLYGLLMAWVLVRYEFPGKRFLDAMVDIPFALPTAVAGLSLSALFAASGWYGAILEPMGIQVVYTIWGVAAAMSFTSIP
FVIRTVQPVLEDLDPQFEQAAVTLGAHPFTIFRRVILPAIAPALLVGVTLSFARSLGEFGAVVFIAGNLPMKTEIASLLA
VIRLEEYDYNGAAVIALTLLAIALVLLGASNLLQARALRYKGKQA

Sequences:

>Translated_285_residues
MIARTAPGPARRSKRVLPGFGLTMGMVLTYLGVIVLLPVVALVLKGADIGPARFWAIVTAPRTLAALRLTLLAAGIATLV
NTLYGLLMAWVLVRYEFPGKRFLDAMVDIPFALPTAVAGLSLSALFAASGWYGAILEPMGIQVVYTIWGVAAAMSFTSIP
FVIRTVQPVLEDLDPQFEQAAVTLGAHPFTIFRRVILPAIAPALLVGVTLSFARSLGEFGAVVFIAGNLPMKTEIASLLA
VIRLEEYDYNGAAVIALTLLAIALVLLGASNLLQARALRYKGKQA
>Mature_285_residues
MIARTAPGPARRSKRVLPGFGLTMGMVLTYLGVIVLLPVVALVLKGADIGPARFWAIVTAPRTLAALRLTLLAAGIATLV
NTLYGLLMAWVLVRYEFPGKRFLDAMVDIPFALPTAVAGLSLSALFAASGWYGAILEPMGIQVVYTIWGVAAAMSFTSIP
FVIRTVQPVLEDLDPQFEQAAVTLGAHPFTIFRRVILPAIAPALLVGVTLSFARSLGEFGAVVFIAGNLPMKTEIASLLA
VIRLEEYDYNGAAVIALTLLAIALVLLGASNLLQARALRYKGKQA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=267, Percent_Identity=54.3071161048689, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI87082099, Length=286, Percent_Identity=34.2657342657343, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786980, Length=230, Percent_Identity=35.2173913043478, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787368, Length=212, Percent_Identity=32.5471698113208, Blast_Score=71, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30302; Mature: 30302

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARTAPGPARRSKRVLPGFGLTMGMVLTYLGVIVLLPVVALVLKGADIGPARFWAIVTA
CCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEHCC
PRTLAALRLTLLAAGIATLVNTLYGLLMAWVLVRYEFPGKRFLDAMVDIPFALPTAVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
SLSALFAASGWYGAILEPMGIQVVYTIWGVAAAMSFTSIPFVIRTVQPVLEDLDPQFEQA
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AVTLGAHPFTIFRRVILPAIAPALLVGVTLSFARSLGEFGAVVFIAGNLPMKTEIASLLA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
VIRLEEYDYNGAAVIALTLLAIALVLLGASNLLQARALRYKGKQA
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIARTAPGPARRSKRVLPGFGLTMGMVLTYLGVIVLLPVVALVLKGADIGPARFWAIVTA
CCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEHCC
PRTLAALRLTLLAAGIATLVNTLYGLLMAWVLVRYEFPGKRFLDAMVDIPFALPTAVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
SLSALFAASGWYGAILEPMGIQVVYTIWGVAAAMSFTSIPFVIRTVQPVLEDLDPQFEQA
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AVTLGAHPFTIFRRVILPAIAPALLVGVTLSFARSLGEFGAVVFIAGNLPMKTEIASLLA
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
VIRLEEYDYNGAAVIALTLLAIALVLLGASNLLQARALRYKGKQA
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]