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The map label for this gene is xylF [H]

Identifier: 77465709

GI number: 77465709

Start: 822302

End: 823351

Strand: Direct

Name: xylF [H]

Synonym: RSP_3701

Alternate gene names: 77465709

Gene position: 822302-823351 (Clockwise)

Preceding gene: 77465707

Following gene: 77465710

Centisome position: 87.2

GC content: 68.0

Gene sequence:

>1050_bases
ATGACCAGCAGAACCTTCGCCCTGGCTGCGGCCTCGGGCCTCGTGGCCGCCCTTTTCGGCGCCGCCGCTTCCGCGCAGGA
GGCCGCCACCGTGGCCTTCCTGATGCCCGACCAGGCATCCACCCGCTACGAGGAGCACGACTTCCCCGGCTTCCAGAAGT
CGATGGGCGAGCTCTGCGCCGACTGCACGGTGATCTACCAGAACGCCAACGGCGACGTGGCGCTCCAGCAGCAGCAGTTC
AACTCGGTGATCGCGCAGGGCGCCAAGATCGTCGTGCTCGATCCGGTCGATTCGGCCGCCGCCGCCTCGATGGTCGAGAT
CGCCCATTCGCAGGATGTGAAGGTGATCGCCTATGACCGGCCGATCCCGGCCACGCCCGCGGATTACTACGTCTCCTTCG
ATAACAAGGGCATCGGCCAGGCCATCGCCCAGTCGCTCGTCGATCATCTGAAGGCCACCGGCGTGCCGGACGGCGCGGGC
GTCCTGCAGATCAACGGCTCGCCCACCGATGCGGCCGCGGGCCTCATTCGCGACGGGATCGACGCGGCGCTCGACGCATC
GAGCTACAAGACGCTGGCCGAGTTCGACACGCCGGACTGGGCCCCGCCGAAGGCGCAGGAATGGGCCGCGGGCCAGATCA
CCCGCTTCGGCGACGAGATCAAGGGCGTGGTCGCGGCCAATGACGGCACCGCCGGCGGCGCCATCGCGGCCTTCAAGGCG
GCGGGCGTGGATCCGGTTCCGCCGGTCACCGGCAACGACGCCACCATCGCGGCGCTGCAGCTCATCATCTCGGGCGACCA
GTACAACACCATCTCGAAACCCTCCGAGATCGTGGCCGAGGCCGCGGCGAAGGTGGTCGTGACCTTCCTCAAGGGCGAGA
CCCCCGAGGCCAAGACCACGCTCTACGACACGCCGGCCGAGCTCTTCGTGCCTGCGGTGGTGACGGCCGAGAACATCAAG
GCCGAGATCTTCGACAAGGGCATCCAGACCGCGGCGGAAGTCTGCACCGGCGAATATGCCGAAGGCTGCGCCAAGCTCGG
CATCCAGTGA

Upstream 100 bases:

>100_bases
AGGATCTGCGGCCCCACGCCGCCCTGCGACCCGCGACGGCGGGCGCGGTGCTGTCTCCATCCCGGTCGGCCCGGAGGCGC
CTGACATGTGGAGGAGAGAC

Downstream 100 bases:

>100_bases
GCCCCCCGGGCGCCTGTCCCCTGAGGACGGGCGCCCCCTTCCAGAAAGGCAGATCGCGATGACCCTGATCTCCGCCGAGG
AGCGCGGCCAGCCCATCCTG

Product: ABC sugar transporter, periplasmic binding protein

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTSRTFALAAASGLVAALFGAAASAQEAATVAFLMPDQASTRYEEHDFPGFQKSMGELCADCTVIYQNANGDVALQQQQF
NSVIAQGAKIVVLDPVDSAAAASMVEIAHSQDVKVIAYDRPIPATPADYYVSFDNKGIGQAIAQSLVDHLKATGVPDGAG
VLQINGSPTDAAAGLIRDGIDAALDASSYKTLAEFDTPDWAPPKAQEWAAGQITRFGDEIKGVVAANDGTAGGAIAAFKA
AGVDPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAEAAAKVVVTFLKGETPEAKTTLYDTPAELFVPAVVTAENIK
AEIFDKGIQTAAEVCTGEYAEGCAKLGIQ

Sequences:

>Translated_349_residues
MTSRTFALAAASGLVAALFGAAASAQEAATVAFLMPDQASTRYEEHDFPGFQKSMGELCADCTVIYQNANGDVALQQQQF
NSVIAQGAKIVVLDPVDSAAAASMVEIAHSQDVKVIAYDRPIPATPADYYVSFDNKGIGQAIAQSLVDHLKATGVPDGAG
VLQINGSPTDAAAGLIRDGIDAALDASSYKTLAEFDTPDWAPPKAQEWAAGQITRFGDEIKGVVAANDGTAGGAIAAFKA
AGVDPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAEAAAKVVVTFLKGETPEAKTTLYDTPAELFVPAVVTAENIK
AEIFDKGIQTAAEVCTGEYAEGCAKLGIQ
>Mature_348_residues
TSRTFALAAASGLVAALFGAAASAQEAATVAFLMPDQASTRYEEHDFPGFQKSMGELCADCTVIYQNANGDVALQQQQFN
SVIAQGAKIVVLDPVDSAAAASMVEIAHSQDVKVIAYDRPIPATPADYYVSFDNKGIGQAIAQSLVDHLKATGVPDGAGV
LQINGSPTDAAAGLIRDGIDAALDASSYKTLAEFDTPDWAPPKAQEWAAGQITRFGDEIKGVVAANDGTAGGAIAAFKAA
GVDPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAEAAAKVVVTFLKGETPEAKTTLYDTPAELFVPAVVTAENIKA
EIFDKGIQTAAEVCTGEYAEGCAKLGIQ

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=321, Percent_Identity=32.7102803738318, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1788473, Length=283, Percent_Identity=26.5017667844523, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35987; Mature: 35855

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRTFALAAASGLVAALFGAAASAQEAATVAFLMPDQASTRYEEHDFPGFQKSMGELCA
CCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHC
DCTVIYQNANGDVALQQQQFNSVIAQGAKIVVLDPVDSAAAASMVEIAHSQDVKVIAYDR
CEEEEEECCCCCEEEHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECC
PIPATPADYYVSFDNKGIGQAIAQSLVDHLKATGVPDGAGVLQINGSPTDAAAGLIRDGI
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHCH
DAALDASSYKTLAEFDTPDWAPPKAQEWAAGQITRFGDEIKGVVAANDGTAGGAIAAFKA
HHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCHHHHHHH
AGVDPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAEAAAKVVVTFLKGETPEAKTT
CCCCCCCCCCCCCHHHHHEEHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCE
LYDTPAELFVPAVVTAENIKAEIFDKGIQTAAEVCTGEYAEGCAKLGIQ
EECCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
TSRTFALAAASGLVAALFGAAASAQEAATVAFLMPDQASTRYEEHDFPGFQKSMGELCA
CCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHC
DCTVIYQNANGDVALQQQQFNSVIAQGAKIVVLDPVDSAAAASMVEIAHSQDVKVIAYDR
CEEEEEECCCCCEEEHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECC
PIPATPADYYVSFDNKGIGQAIAQSLVDHLKATGVPDGAGVLQINGSPTDAAAGLIRDGI
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHCH
DAALDASSYKTLAEFDTPDWAPPKAQEWAAGQITRFGDEIKGVVAANDGTAGGAIAAFKA
HHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCHHHHHHH
AGVDPVPPVTGNDATIAALQLIISGDQYNTISKPSEIVAEAAAKVVVTFLKGETPEAKTT
CCCCCCCCCCCCCHHHHHEEHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCE
LYDTPAELFVPAVVTAENIKAEIFDKGIQTAAEVCTGEYAEGCAKLGIQ
EECCCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]