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The map label for this gene is gatA [H]

Identifier: 77465526

GI number: 77465526

Start: 606532

End: 608004

Strand: Direct

Name: gatA [H]

Synonym: RSP_3526

Alternate gene names: 77465526

Gene position: 606532-608004 (Clockwise)

Preceding gene: 77465520

Following gene: 77465527

Centisome position: 64.32

GC content: 69.04

Gene sequence:

>1473_bases
ATGCGCCAGGTTTCCCCCGCTGCCGAAGGCCCCTCCCCCATGACCGAGACGCCCCTCGCCTACCTCAGCGCCGCGCAGCT
GACGGCCCTTTATCGCGCCCGGAAAATCTCGCCCGTCGAAGTGGTTACGGACGCCCTCGCGCGGATCGAGGCCAGCGGAG
ACAGTCTGAACGCGGTCTGCTTCACCTACCCGGAGGAGGCGCTGGAGGAAGCACGCGCCTCCGAGAAGCGATATATGTCC
GGCGCACAGAAACCGGGCATGATCGATGGCATCCCTGCCGCGCTGAAGGACGAGACCATGATGGCGGGCAAGGTCACGAC
CTACGGCTCGCTCCTCTACGCGGACCATGTGGCTGCGAAGAGCGCGCCGGTCGTGCAGCGGCTGGTCGAGGCGGGCGCGG
TGATCCATGCCCGCACCACCACGCCCGAATTCTCCTGCGCTCCCTTCTGTCACTCGCGGCAGTGGGGCGTGACGCGCAAC
CCGTGGAACCTCGCCTTCACGCCCGGCGGCTCCTCGGGCGGCTCCGGGGCGGCGCTCGCGGCGGGGCTCGCGCCGCTGGC
CACGGGCTCGGACATCGGCGGCTCGATTCGGATCCCGGCCTCCGCCTCGGGGGTGGTGGGCTTCAAGCCGCCCTACGGCC
GTGTGCCCTCGACCCCGCCCTTCAACCTCGATCCCTACAACCATCCGGGCCCCATGGCGCGCACGGTCGAGGATTGCCTG
CTGATGCAGAACGTGCTCGCCGGCCCCCATCCGGAGGACATCGCCTCGCTGAAGCCCAAGCTCGAGCTGCGGCTCGACCG
GTCGGGGGTGAAGGGCTGGAAGATCGCCTATTCGCTGGATTTCGGCTTCATGGAGATCGATCCGGCGGTGCGGGCCAACA
CGCTTGCGGCGCTGGACGTGTTCCGCGCGCTCGGCGCCGAGGTCACGGAGGTGGCGCTGCCCTGGTCCTGGGAGGTCTAT
CGGGCCGCCGCCACCCATCACAAGGCGCTGTTCGGCGCCTGGCTTGCCGAGTATCTGGACGAGCGCGAGGAGCGGCTCAC
CTCCTACGCCCGCCGCTTCGCCCGCGACGCGCTCGCCACCACCACCCGCGACTATCTCGCCGGGATGGAGATCGAGGGCC
GGATCTGGTCGCATTTCGGCCCGATGATGGAGGGGTTCGACCTGTTCCTCTGCCCCACCCTCGCCATTCCCGCCGTGCCG
GCCGAGTTCGACTTCTCCGATCCGCTGATCATCAACGGAGAAGAGATCGACTCCTATCTCGGCTGGACGATGGCCTGGCC
CTTCAACATGCTCAGCCGCTGCCCGGTCCTGTCGCTGCCCTCGGGCCGTGCGCCGAACGGCGTGCCCACCGGCATCCAGC
TGGTGGGCCGGACCTATGAAGACCAGAGCGTCTTCACGGCGGGCCTCGCCTACGAGACCGCTCTCGGCGGCTGGTATGGC
ACCGCCGCAACCCGTCCCTCCCTGCCCTTCTGA

Upstream 100 bases:

>100_bases
CAGCCTTCCGACCTGCCGGTGATCCTTATGGAAATCACGATACGCAGAAATCGAATCCTGTCAACAAACTGATTGACCAA
TCAATCGAGCAACCACCAGA

Downstream 100 bases:

>100_bases
GGTCCCGATGAAGACGATCTTCTCCGACAAGCACGCGCTGCAGAGCGTGGAGCGCGAATTCTACCGGGGCGGCTGGGTGG
AGGCGTTCGAGATCCCCCGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRQVSPAAEGPSPMTETPLAYLSAAQLTALYRARKISPVEVVTDALARIEASGDSLNAVCFTYPEEALEEARASEKRYMS
GAQKPGMIDGIPAALKDETMMAGKVTTYGSLLYADHVAAKSAPVVQRLVEAGAVIHARTTTPEFSCAPFCHSRQWGVTRN
PWNLAFTPGGSSGGSGAALAAGLAPLATGSDIGGSIRIPASASGVVGFKPPYGRVPSTPPFNLDPYNHPGPMARTVEDCL
LMQNVLAGPHPEDIASLKPKLELRLDRSGVKGWKIAYSLDFGFMEIDPAVRANTLAALDVFRALGAEVTEVALPWSWEVY
RAAATHHKALFGAWLAEYLDEREERLTSYARRFARDALATTTRDYLAGMEIEGRIWSHFGPMMEGFDLFLCPTLAIPAVP
AEFDFSDPLIINGEEIDSYLGWTMAWPFNMLSRCPVLSLPSGRAPNGVPTGIQLVGRTYEDQSVFTAGLAYETALGGWYG
TAATRPSLPF

Sequences:

>Translated_490_residues
MRQVSPAAEGPSPMTETPLAYLSAAQLTALYRARKISPVEVVTDALARIEASGDSLNAVCFTYPEEALEEARASEKRYMS
GAQKPGMIDGIPAALKDETMMAGKVTTYGSLLYADHVAAKSAPVVQRLVEAGAVIHARTTTPEFSCAPFCHSRQWGVTRN
PWNLAFTPGGSSGGSGAALAAGLAPLATGSDIGGSIRIPASASGVVGFKPPYGRVPSTPPFNLDPYNHPGPMARTVEDCL
LMQNVLAGPHPEDIASLKPKLELRLDRSGVKGWKIAYSLDFGFMEIDPAVRANTLAALDVFRALGAEVTEVALPWSWEVY
RAAATHHKALFGAWLAEYLDEREERLTSYARRFARDALATTTRDYLAGMEIEGRIWSHFGPMMEGFDLFLCPTLAIPAVP
AEFDFSDPLIINGEEIDSYLGWTMAWPFNMLSRCPVLSLPSGRAPNGVPTGIQLVGRTYEDQSVFTAGLAYETALGGWYG
TAATRPSLPF
>Mature_490_residues
MRQVSPAAEGPSPMTETPLAYLSAAQLTALYRARKISPVEVVTDALARIEASGDSLNAVCFTYPEEALEEARASEKRYMS
GAQKPGMIDGIPAALKDETMMAGKVTTYGSLLYADHVAAKSAPVVQRLVEAGAVIHARTTTPEFSCAPFCHSRQWGVTRN
PWNLAFTPGGSSGGSGAALAAGLAPLATGSDIGGSIRIPASASGVVGFKPPYGRVPSTPPFNLDPYNHPGPMARTVEDCL
LMQNVLAGPHPEDIASLKPKLELRLDRSGVKGWKIAYSLDFGFMEIDPAVRANTLAALDVFRALGAEVTEVALPWSWEVY
RAAATHHKALFGAWLAEYLDEREERLTSYARRFARDALATTTRDYLAGMEIEGRIWSHFGPMMEGFDLFLCPTLAIPAVP
AEFDFSDPLIINGEEIDSYLGWTMAWPFNMLSRCPVLSLPSGRAPNGVPTGIQLVGRTYEDQSVFTAGLAYETALGGWYG
TAATRPSLPF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=508, Percent_Identity=25.7874015748031, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=27.1287128712871, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI166795287, Length=195, Percent_Identity=35.3846153846154, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17537465, Length=254, Percent_Identity=29.9212598425197, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=438, Percent_Identity=25.7990867579909, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=310, Percent_Identity=28.7096774193548, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=261, Percent_Identity=29.8850574712644, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=450, Percent_Identity=24.4444444444444, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=260, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=260, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=240, Percent_Identity=28.3333333333333, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=129, Percent_Identity=41.8604651162791, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=399, Percent_Identity=24.5614035087719, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356731, Length=510, Percent_Identity=26.8627450980392, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550774, Length=249, Percent_Identity=32.9317269076305, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648435, Length=249, Percent_Identity=32.9317269076305, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648437, Length=249, Percent_Identity=32.9317269076305, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078093, Length=523, Percent_Identity=25.6214149139579, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648113, Length=487, Percent_Identity=28.3367556468172, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=24.5098039215686, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24648441, Length=166, Percent_Identity=34.9397590361446, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648439, Length=166, Percent_Identity=34.9397590361446, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24652985, Length=254, Percent_Identity=31.1023622047244, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922090, Length=254, Percent_Identity=31.1023622047244, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24652981, Length=254, Percent_Identity=31.1023622047244, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24652983, Length=254, Percent_Identity=31.1023622047244, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 52725; Mature: 52725

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQVSPAAEGPSPMTETPLAYLSAAQLTALYRARKISPVEVVTDALARIEASGDSLNAVC
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEE
FTYPEEALEEARASEKRYMSGAQKPGMIDGIPAALKDETMMAGKVTTYGSLLYADHVAAK
EECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHEECCHHHHHHHHHHHHHHHC
SAPVVQRLVEAGAVIHARTTTPEFSCAPFCHSRQWGVTRNPWNLAFTPGGSSGGSGAALA
CCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHH
AGLAPLATGSDIGGSIRIPASASGVVGFKPPYGRVPSTPPFNLDPYNHPGPMARTVEDCL
HCCHHCCCCCCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LMQNVLAGPHPEDIASLKPKLELRLDRSGVKGWKIAYSLDFGFMEIDPAVRANTLAALDV
HHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEECCHHHHHHHHHHHH
FRALGAEVTEVALPWSWEVYRAAATHHKALFGAWLAEYLDEREERLTSYARRFARDALAT
HHHHCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTRDYLAGMEIEGRIWSHFGPMMEGFDLFLCPTLAIPAVPAEFDFSDPLIINGEEIDSYL
HHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCCEEECHHHHHHHH
GWTMAWPFNMLSRCPVLSLPSGRAPNGVPTGIQLVGRTYEDQSVFTAGLAYETALGGWYG
CHHHCCHHHHHHCCCEEECCCCCCCCCCCCCHHEECCCCCCCHHEEHHHHHHHHHCCCCC
TAATRPSLPF
CCCCCCCCCC
>Mature Secondary Structure
MRQVSPAAEGPSPMTETPLAYLSAAQLTALYRARKISPVEVVTDALARIEASGDSLNAVC
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEE
FTYPEEALEEARASEKRYMSGAQKPGMIDGIPAALKDETMMAGKVTTYGSLLYADHVAAK
EECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCHHEECCHHHHHHHHHHHHHHHC
SAPVVQRLVEAGAVIHARTTTPEFSCAPFCHSRQWGVTRNPWNLAFTPGGSSGGSGAALA
CCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHH
AGLAPLATGSDIGGSIRIPASASGVVGFKPPYGRVPSTPPFNLDPYNHPGPMARTVEDCL
HCCHHCCCCCCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LMQNVLAGPHPEDIASLKPKLELRLDRSGVKGWKIAYSLDFGFMEIDPAVRANTLAALDV
HHHHHHCCCCHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCEEECCHHHHHHHHHHHH
FRALGAEVTEVALPWSWEVYRAAATHHKALFGAWLAEYLDEREERLTSYARRFARDALAT
HHHHCCCHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTRDYLAGMEIEGRIWSHFGPMMEGFDLFLCPTLAIPAVPAEFDFSDPLIINGEEIDSYL
HHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCCEEECHHHHHHHH
GWTMAWPFNMLSRCPVLSLPSGRAPNGVPTGIQLVGRTYEDQSVFTAGLAYETALGGWYG
CHHHCCHHHHHHCCCEEECCCCCCCCCCCCCHHEECCCCCCCHHEEHHHHHHHHHCCCCC
TAATRPSLPF
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]