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The map label for this gene is uvrD [H]

Identifier: 77461864

GI number: 77461864

Start: 6325758

End: 6327941

Strand: Direct

Name: uvrD [H]

Synonym: Pfl01_5644

Alternate gene names: 77461864

Gene position: 6325758-6327941 (Clockwise)

Preceding gene: 77461861

Following gene: 77461865

Centisome position: 98.25

GC content: 62.09

Gene sequence:

>2184_bases
ATGCGCGATGATCTCTCCCTTCTGCTGAACTCCCTCAACGATGCCCAACGCCAGGCCGTAGCGGCTCCCGTTGGTCGTCA
ATTGGTCCTGGCCGGTGCCGGCTCCGGCAAAACCCGAGTGCTGGTGCACCGTATCGCCTGGTTGATCCAGGTCGAGAACG
CCTCGCCCCACTCGATTCTGTCGGTGACCTTCACCAACAAGGCCGCTGCCGAGATGCGTCACCGCATCGAACAATTGCTG
GGCATCAATCCGGCCGGCATGTGGGTCGGCACCTTCCACGGCCTGGCGCACCGCCTGCTGCGGGCGCACTGGCAGGAAGC
CGGGCTGAGCCAGACCTTCCAGATTCTCGACAGCGACGACCAGCAACGCTTGGTCAAGCGGGTGATCCGCGAGCTGGGGC
TGGACGAGCAACGCTGGCCGGCCCGTCAGGCCCAATGGTTCATCAACGGGCAGAAAGACGAAGGTCTGCGTCCGCAACAC
ATTCAAGCCAGCGGCGATCTGTTCCTGGCCACCATGCGCGGCATTTATGAAGCGTACGAGGCCGCATGCCAGCGTGCCGG
CGTCATCGATTTCTCCGAACTGCTGCTGCGCGCCCTCGACCTGTGGCGCGACCACCCGGGCCTGCTGGCGCACTACCAGA
AGCGCTTCCGACACATTCTGGTGGACGAATTCCAGGACACCAACGCCGTGCAGTACGCCTGGTTGCGTCTGCTGGGCAAG
GGCGGCGACAGCCTGATGGTGGTCGGCGACGACGACCAGTCGATCTACGGCTGGCGCGGCGCGAAAATCGAGAACATCCA
CCAGTATTCTTCGGACTTCCCGGACTCGGTGACCATCCGTCTGGAGCAGAACTACCGCTCCACGGCCGGCATCCTCAAGG
CCGCCAACGCCCTGATCGCCAACAACACCGGGCGTCTGGGCAAAGAGCTGTGGACCGATGGCGGCGATGGCGAAGCGATC
AATCTTTACGCCGCCTTCAACGAGCACGATGAAGCACGCTACGTTGTCGAAACCATCGAAAGCGCGCTGAAAACCGGCTT
GGCCCGCAGCGATATCGCGATTCTCTACCGCTCCAACGCCCAATCGCGCGTTCTGGAAGAAGCCTTGCTGCGTGAACGCA
TTCCGTACCGCATCTATGGCGGCCAGCGCTTCTTCGAGCGGGCGGAAATCAAGAACGCCATGGCTTACCTGCGTTTGCTG
GAAGGTCGCGGCAATGATGCGGCCCTGGAGCGGGTGATCAACGTCCCAGCGCGCGGCATCGGCGAGAAAACCGTCGAAGC
GATCCGCGACCATGCCCGTCACAGCGATGTGTCGATGTGGGAAGCCATGGGCCAGCTGGTGGCCAACAAAGGCCTGACCG
GTCGCGCCGCCGGTGCGCTGGGCGCGTTTATCGAACTGATCGAAAACCTGGCTGCCAAGTGCGCCGAGATGCCGTTGCAC
CTGATGACCCAGACCGTCATCGAGCAATCCGGACTGATCGCCTACCACGAAGCGGAAAAAGGCGAGAAAGGCCAGGCCCG
GGTGGAAAACCTTGAGGAACTGGTCAGCGCTGCGCGCAACTTCGAGAACACCGAAGAAGACGAAGAACTGACGCCACTGG
CGGCATTTCTCGGCCACGCTTCACTGGAGGCCGGTGACACCCAGGCCGACGAGCACGAAGACAGCATTCAGTTGATGACC
CTGCACAGCGCCAAGGGCCTGGAATTCCCTTACGTGTTCCTGGTGGGCATGGAAGAAGGCTTGTTCCCGCACAAGATGAG
CCTGGAAGAGCCGGGACGCCTTGAGGAAGAACGGCGCCTGGCCTATGTCGGCATTACCCGGGCGATGCAGAATCTGGTGA
TGACCTATGCTGAAACCCGACGCCTGTACGGCAGCGAAACCTACAACAAGGTGTCGCGTTTCGTACGGGAAGTACCGAAA
GGCCTGATTCAGGAAGTACGCCTGTCCAACAGCGTCAGCCGGCCGTTCGGCGGTAACCAGTCGATGGGCGGCAGCAACCT
GTTCAGCGGCAGCGAGATTCCGGAAACCGGCCTGAGCCTTGGCCAGGCTGTCCGTCACTCGATCTTCGGCGACGGCGTGA
TCCTCAACTTCGAAGGCGCCGGCGCCCAGGCCCGGGTGCAGGTGAATTTCGCCGAAGGCAGCAAGTGGCTGATGCTCGGG
TACGCGAAGCTGGAAGCCATATAA

Upstream 100 bases:

>100_bases
GCCTGTAGCCGGTGACCGACCGGTCGTCATCGGCAGCACTTTCATCGGCTAATGCAAAGCTCGGTTTGCCCGGGCCTGCG
GCGCACCCTAGAATGCCCCG

Downstream 100 bases:

>100_bases
GTCACCGTTCGTTCCCGCCCCCGGCGCGGGAACGAACACTGGAGGCAACGTCGATGGGCACTGGCTTCTTTTCCTCCTGG
ACATTCTGGGCCCTGCTCTC

Product: DNA-dependent helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 727; Mature: 727

Protein sequence:

>727_residues
MRDDLSLLLNSLNDAQRQAVAAPVGRQLVLAGAGSGKTRVLVHRIAWLIQVENASPHSILSVTFTNKAAAEMRHRIEQLL
GINPAGMWVGTFHGLAHRLLRAHWQEAGLSQTFQILDSDDQQRLVKRVIRELGLDEQRWPARQAQWFINGQKDEGLRPQH
IQASGDLFLATMRGIYEAYEAACQRAGVIDFSELLLRALDLWRDHPGLLAHYQKRFRHILVDEFQDTNAVQYAWLRLLGK
GGDSLMVVGDDDQSIYGWRGAKIENIHQYSSDFPDSVTIRLEQNYRSTAGILKAANALIANNTGRLGKELWTDGGDGEAI
NLYAAFNEHDEARYVVETIESALKTGLARSDIAILYRSNAQSRVLEEALLRERIPYRIYGGQRFFERAEIKNAMAYLRLL
EGRGNDAALERVINVPARGIGEKTVEAIRDHARHSDVSMWEAMGQLVANKGLTGRAAGALGAFIELIENLAAKCAEMPLH
LMTQTVIEQSGLIAYHEAEKGEKGQARVENLEELVSAARNFENTEEDEELTPLAAFLGHASLEAGDTQADEHEDSIQLMT
LHSAKGLEFPYVFLVGMEEGLFPHKMSLEEPGRLEEERRLAYVGITRAMQNLVMTYAETRRLYGSETYNKVSRFVREVPK
GLIQEVRLSNSVSRPFGGNQSMGGSNLFSGSEIPETGLSLGQAVRHSIFGDGVILNFEGAGAQARVQVNFAEGSKWLMLG
YAKLEAI

Sequences:

>Translated_727_residues
MRDDLSLLLNSLNDAQRQAVAAPVGRQLVLAGAGSGKTRVLVHRIAWLIQVENASPHSILSVTFTNKAAAEMRHRIEQLL
GINPAGMWVGTFHGLAHRLLRAHWQEAGLSQTFQILDSDDQQRLVKRVIRELGLDEQRWPARQAQWFINGQKDEGLRPQH
IQASGDLFLATMRGIYEAYEAACQRAGVIDFSELLLRALDLWRDHPGLLAHYQKRFRHILVDEFQDTNAVQYAWLRLLGK
GGDSLMVVGDDDQSIYGWRGAKIENIHQYSSDFPDSVTIRLEQNYRSTAGILKAANALIANNTGRLGKELWTDGGDGEAI
NLYAAFNEHDEARYVVETIESALKTGLARSDIAILYRSNAQSRVLEEALLRERIPYRIYGGQRFFERAEIKNAMAYLRLL
EGRGNDAALERVINVPARGIGEKTVEAIRDHARHSDVSMWEAMGQLVANKGLTGRAAGALGAFIELIENLAAKCAEMPLH
LMTQTVIEQSGLIAYHEAEKGEKGQARVENLEELVSAARNFENTEEDEELTPLAAFLGHASLEAGDTQADEHEDSIQLMT
LHSAKGLEFPYVFLVGMEEGLFPHKMSLEEPGRLEEERRLAYVGITRAMQNLVMTYAETRRLYGSETYNKVSRFVREVPK
GLIQEVRLSNSVSRPFGGNQSMGGSNLFSGSEIPETGLSLGQAVRHSIFGDGVILNFEGAGAQARVQVNFAEGSKWLMLG
YAKLEAI
>Mature_727_residues
MRDDLSLLLNSLNDAQRQAVAAPVGRQLVLAGAGSGKTRVLVHRIAWLIQVENASPHSILSVTFTNKAAAEMRHRIEQLL
GINPAGMWVGTFHGLAHRLLRAHWQEAGLSQTFQILDSDDQQRLVKRVIRELGLDEQRWPARQAQWFINGQKDEGLRPQH
IQASGDLFLATMRGIYEAYEAACQRAGVIDFSELLLRALDLWRDHPGLLAHYQKRFRHILVDEFQDTNAVQYAWLRLLGK
GGDSLMVVGDDDQSIYGWRGAKIENIHQYSSDFPDSVTIRLEQNYRSTAGILKAANALIANNTGRLGKELWTDGGDGEAI
NLYAAFNEHDEARYVVETIESALKTGLARSDIAILYRSNAQSRVLEEALLRERIPYRIYGGQRFFERAEIKNAMAYLRLL
EGRGNDAALERVINVPARGIGEKTVEAIRDHARHSDVSMWEAMGQLVANKGLTGRAAGALGAFIELIENLAAKCAEMPLH
LMTQTVIEQSGLIAYHEAEKGEKGQARVENLEELVSAARNFENTEEDEELTPLAAFLGHASLEAGDTQADEHEDSIQLMT
LHSAKGLEFPYVFLVGMEEGLFPHKMSLEEPGRLEEERRLAYVGITRAMQNLVMTYAETRRLYGSETYNKVSRFVREVPK
GLIQEVRLSNSVSRPFGGNQSMGGSNLFSGSEIPETGLSLGQAVRHSIFGDGVILNFEGAGAQARVQVNFAEGSKWLMLG
YAKLEAI

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=725, Percent_Identity=62.7586206896552, Blast_Score=940, Evalue=0.0,
Organism=Escherichia coli, GI48994965, Length=642, Percent_Identity=39.5638629283489, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=365, Percent_Identity=30.1369863013699, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=669, Percent_Identity=28.101644245142, Blast_Score=207, Evalue=5e-54,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005753
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 80898; Mature: 80898

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDDLSLLLNSLNDAQRQAVAAPVGRQLVLAGAGSGKTRVLVHRIAWLIQVENASPHSIL
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHEEECCCCCCEEE
SVTFTNKAAAEMRHRIEQLLGINPAGMWVGTFHGLAHRLLRAHWQEAGLSQTFQILDSDD
EEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
QQRLVKRVIRELGLDEQRWPARQAQWFINGQKDEGLRPQHIQASGDLFLATMRGIYEAYE
HHHHHHHHHHHCCCCCCCCCCHHHHEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHH
AACQRAGVIDFSELLLRALDLWRDHPGLLAHYQKRFRHILVDEFQDTNAVQYAWLRLLGK
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
GGDSLMVVGDDDQSIYGWRGAKIENIHQYSSDFPDSVTIRLEQNYRSTAGILKAANALIA
CCCEEEEEECCCCCEECCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHC
NNTGRLGKELWTDGGDGEAINLYAAFNEHDEARYVVETIESALKTGLARSDIAILYRSNA
CCCCHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
QSRVLEEALLRERIPYRIYGGQRFFERAEIKNAMAYLRLLEGRGNDAALERVINVPARGI
HHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
GEKTVEAIRDHARHSDVSMWEAMGQLVANKGLTGRAAGALGAFIELIENLAAKCAEMPLH
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
LMTQTVIEQSGLIAYHEAEKGEKGQARVENLEELVSAARNFENTEEDEELTPLAAFLGHA
HHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
SLEAGDTQADEHEDSIQLMTLHSAKGLEFPYVFLVGMEEGLFPHKMSLEEPGRLEEERRL
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHH
AYVGITRAMQNLVMTYAETRRLYGSETYNKVSRFVREVPKGLIQEVRLSNSVSRPFGGNQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SMGGSNLFSGSEIPETGLSLGQAVRHSIFGDGVILNFEGAGAQARVQVNFAEGSKWLMLG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCCEEEEE
YAKLEAI
HHHHHCC
>Mature Secondary Structure
MRDDLSLLLNSLNDAQRQAVAAPVGRQLVLAGAGSGKTRVLVHRIAWLIQVENASPHSIL
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHEEECCCCCCEEE
SVTFTNKAAAEMRHRIEQLLGINPAGMWVGTFHGLAHRLLRAHWQEAGLSQTFQILDSDD
EEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
QQRLVKRVIRELGLDEQRWPARQAQWFINGQKDEGLRPQHIQASGDLFLATMRGIYEAYE
HHHHHHHHHHHCCCCCCCCCCHHHHEEECCCCCCCCCCCEECCCCCCHHHHHHHHHHHHH
AACQRAGVIDFSELLLRALDLWRDHPGLLAHYQKRFRHILVDEFQDTNAVQYAWLRLLGK
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
GGDSLMVVGDDDQSIYGWRGAKIENIHQYSSDFPDSVTIRLEQNYRSTAGILKAANALIA
CCCEEEEEECCCCCEECCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHC
NNTGRLGKELWTDGGDGEAINLYAAFNEHDEARYVVETIESALKTGLARSDIAILYRSNA
CCCCHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
QSRVLEEALLRERIPYRIYGGQRFFERAEIKNAMAYLRLLEGRGNDAALERVINVPARGI
HHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
GEKTVEAIRDHARHSDVSMWEAMGQLVANKGLTGRAAGALGAFIELIENLAAKCAEMPLH
CHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
LMTQTVIEQSGLIAYHEAEKGEKGQARVENLEELVSAARNFENTEEDEELTPLAAFLGHA
HHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
SLEAGDTQADEHEDSIQLMTLHSAKGLEFPYVFLVGMEEGLFPHKMSLEEPGRLEEERRL
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHH
AYVGITRAMQNLVMTYAETRRLYGSETYNKVSRFVREVPKGLIQEVRLSNSVSRPFGGNQ
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SMGGSNLFSGSEIPETGLSLGQAVRHSIFGDGVILNFEGAGAQARVQVNFAEGSKWLMLG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECCCCCEEEEE
YAKLEAI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6379604; 2943729; 1379743; 9278503; 6324092; 2254268; 8419285 [H]