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The map label for this gene is dut

Identifier: 77461762

GI number: 77461762

Start: 6210403

End: 6210858

Strand: Direct

Name: dut

Synonym: Pfl01_5541

Alternate gene names: 77461762

Gene position: 6210403-6210858 (Clockwise)

Preceding gene: 77461761

Following gene: 77461763

Centisome position: 96.46

GC content: 62.94

Gene sequence:

>456_bases
ATGCACGCTTTGCAAGCCAAGATCCTCGACCCACGCATCGGTACCGAATTCCCGCTGCCGCAATACGCCACACCGGGCTC
CGCCGGCCTCGACCTGCGCGCCATGCTGCAAGAGGACATTGTGATCAAGCCGGGTGAAACCGTGCTGATTCCGACCGGTC
TGTCGGTCTATATCGGCGATCCGAACCTCGCCGCGCTGATCCTGCCGCGTTCGGGCATGGGCCATAAGCACGGCATCGTG
TTGGGCAACCTCGTCGGCCTGATCGACTCCGACTACCAGGGCCCGCTGATGGTGTCCTGCTGGAACCGTGGCCAGACCGA
ATTCACCATGCCGGTCGGCGAGCGCCTGGCTCAACTGGTACTGGTACCCGTGGTGCAGGCGCACTTCGAAATGGTTGAAG
AGTTCGTCGAGACCGAGCGCGGCACGGGTGGTTTCGGCCATTCCGGCACCAAGTGA

Upstream 100 bases:

>100_bases
GTGAGCTTCACGCCACCGTTTTCGCCCAGACCAGCAAGAGCAAGATCGCTCGCCAACTGGTCACTTTTATCGCCGAACGT
CTGAACCAGGTTTAATTTAC

Downstream 100 bases:

>100_bases
TCGGCTGAAAACCCGTCGTGGCGAGGAGGCATTCCCCGCCACGAACTTCAAAATGTCATCTGCAAGGTTTAGCGCTGAAA
ATCAACGCATTGGCAGGCCA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MHALQAKILDPRIGTEFPLPQYATPGSAGLDLRAMLQEDIVIKPGETVLIPTGLSVYIGDPNLAALILPRSGMGHKHGIV
LGNLVGLIDSDYQGPLMVSCWNRGQTEFTMPVGERLAQLVLVPVVQAHFEMVEEFVETERGTGGFGHSGTK

Sequences:

>Translated_151_residues
MHALQAKILDPRIGTEFPLPQYATPGSAGLDLRAMLQEDIVIKPGETVLIPTGLSVYIGDPNLAALILPRSGMGHKHGIV
LGNLVGLIDSDYQGPLMVSCWNRGQTEFTMPVGERLAQLVLVPVVQAHFEMVEEFVETERGTGGFGHSGTK
>Mature_151_residues
MHALQAKILDPRIGTEFPLPQYATPGSAGLDLRAMLQEDIVIKPGETVLIPTGLSVYIGDPNLAALILPRSGMGHKHGIV
LGNLVGLIDSDYQGPLMVSCWNRGQTEFTMPVGERLAQLVLVPVVQAHFEMVEEFVETERGTGGFGHSGTK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=138, Percent_Identity=32.6086956521739, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI4503423, Length=138, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=2e-14,
Organism=Homo sapiens, GI70906441, Length=138, Percent_Identity=32.6086956521739, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=66.887417218543, Blast_Score=221, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71988561, Length=131, Percent_Identity=35.1145038167939, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319729, Length=152, Percent_Identity=32.8947368421053, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583610, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19921126, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_PSEPF (Q3K4M6)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_351269.1
- ProteinModelPortal:   Q3K4M6
- SMR:   Q3K4M6
- STRING:   Q3K4M6
- GeneID:   3713631
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5541
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BioCyc:   PFLU205922:PFL_5541-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16245; Mature: 16245

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: BINDING 83-83 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHALQAKILDPRIGTEFPLPQYATPGSAGLDLRAMLQEDIVIKPGETVLIPTGLSVYIGD
CCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCEEEEECCCEEEECC
PNLAALILPRSGMGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRGQTEFTMPVGERLAQLV
CCEEEEEEECCCCCCCCCEEHHHHHHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHH
LVPVVQAHFEMVEEFVETERGTGGFGHSGTK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHALQAKILDPRIGTEFPLPQYATPGSAGLDLRAMLQEDIVIKPGETVLIPTGLSVYIGD
CCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCEEEEECCCEEEECC
PNLAALILPRSGMGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRGQTEFTMPVGERLAQLV
CCEEEEEEECCCCCCCCCEEHHHHHHHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHH
LVPVVQAHFEMVEEFVETERGTGGFGHSGTK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA