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The map label for this gene is pyrB [H]

Identifier: 77461535

GI number: 77461535

Start: 5978512

End: 5979516

Strand: Direct

Name: pyrB [H]

Synonym: Pfl01_5314

Alternate gene names: 77461535

Gene position: 5978512-5979516 (Clockwise)

Preceding gene: 77461534

Following gene: 77461536

Centisome position: 92.86

GC content: 64.98

Gene sequence:

>1005_bases
ATGACGCCTCTAGATACCAAGCGCCCGCTGCAGCTCAATGATCAGGGCCAGCTGCGCCACTTCCTCTCGCTCGACGGCCT
GCGCCGCGAGTTGCTGACGGAAATCCTCGACACCGCCGACTCGTTCCTCGAAGTCGGTGCCCGGGCCGTGAAGAAGGTTC
CGTTGCTGCGCGGCAAGACCGTGTGCAACGTGTTCTTCGAAAACTCGACCCGCACCCGTACCACCTTCGAACTGGCGGCC
CAGCGCCTGTCGGCGGACGTGATCACCCTGAACGTGTCGACCTCTTCGGCCAGCAAGGGCGAAACCCTGCTCGACACCCT
GCGCAACCTCGAGGCCATGGCCGCCGACATGTTCGTCGTGCGCCACGGTGACTCGGGCGCCGCGCACTTCATCGCCGAAC
ATGTCTGCCCGCAAGTGGCGATCATCAACGGCGGCGACGGCCGGCACGCGCACCCGACCCAGGGCATGCTCGACATGCTG
ACCATCCGCCGTCACAAGGGCGGTTTCGAGAACCTCTCGGTGGCCATCGTCGGCGACATCCTGCACTCGCGGGTGGCGCG
CTCGAACATGCTCGCCCTGAAGACCCTCGGCTGCCCGGATATCCGCGTGATCGCACCGAAGACCCTGCTGCCGATCGGCA
TCGAGCAATACGGTGTGAAGGTCTACACCGACATGACCGAAGGACTGAAAGACGTCGACGTGGTGATCATGCTGCGCCTG
CAGCGTGAGCGCATGACTGGCGGTCTGCTGCCGAGCGAAGGCGAGTTCTATCGCCTGTTCGGCCTGACCACCGCGCGCCT
GGCCGGCGCCAAGCCCGATTGCATCGTCATGCACCCGGGGCCGATCAACCGCGGCGTGGAGATCGAGTCGGCGGTGGCCG
ACGGCCCGCACTCGGTGATCCTCAATCAAGTCACCTACGGCATCGCAATCCGTATGGCCGTGCTGTCGATGGCCATGAGC
GGACAGACTGCCCAGCGCCAATTCGAGCAGGAGAACGCCCAGTGA

Upstream 100 bases:

>100_bases
GCTGGCCGCCCACGAGCGGGTCAAGCTGTCCGGGCCGGAGCTGGCCCTCGAACTGCAAGACCTTGCCCTTTAATCCGCCC
TATTGAGAGTCCCCCTCGCG

Downstream 100 bases:

>100_bases
AGCTCAGCATTCTCGGCGCCCGCGTCATCGATCCAAGCAGCGGCCTGGATCAAATCACCGATATTCACGTTGAAGCCTGC
AAGATCGTCGCCCTCGGCGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTPLDTKRPLQLNDQGQLRHFLSLDGLRRELLTEILDTADSFLEVGARAVKKVPLLRGKTVCNVFFENSTRTRTTFELAA
QRLSADVITLNVSTSSASKGETLLDTLRNLEAMAADMFVVRHGDSGAAHFIAEHVCPQVAIINGGDGRHAHPTQGMLDML
TIRRHKGGFENLSVAIVGDILHSRVARSNMLALKTLGCPDIRVIAPKTLLPIGIEQYGVKVYTDMTEGLKDVDVVIMLRL
QRERMTGGLLPSEGEFYRLFGLTTARLAGAKPDCIVMHPGPINRGVEIESAVADGPHSVILNQVTYGIAIRMAVLSMAMS
GQTAQRQFEQENAQ

Sequences:

>Translated_334_residues
MTPLDTKRPLQLNDQGQLRHFLSLDGLRRELLTEILDTADSFLEVGARAVKKVPLLRGKTVCNVFFENSTRTRTTFELAA
QRLSADVITLNVSTSSASKGETLLDTLRNLEAMAADMFVVRHGDSGAAHFIAEHVCPQVAIINGGDGRHAHPTQGMLDML
TIRRHKGGFENLSVAIVGDILHSRVARSNMLALKTLGCPDIRVIAPKTLLPIGIEQYGVKVYTDMTEGLKDVDVVIMLRL
QRERMTGGLLPSEGEFYRLFGLTTARLAGAKPDCIVMHPGPINRGVEIESAVADGPHSVILNQVTYGIAIRMAVLSMAMS
GQTAQRQFEQENAQ
>Mature_333_residues
TPLDTKRPLQLNDQGQLRHFLSLDGLRRELLTEILDTADSFLEVGARAVKKVPLLRGKTVCNVFFENSTRTRTTFELAAQ
RLSADVITLNVSTSSASKGETLLDTLRNLEAMAADMFVVRHGDSGAAHFIAEHVCPQVAIINGGDGRHAHPTQGMLDMLT
IRRHKGGFENLSVAIVGDILHSRVARSNMLALKTLGCPDIRVIAPKTLLPIGIEQYGVKVYTDMTEGLKDVDVVIMLRLQ
RERMTGGLLPSEGEFYRLFGLTTARLAGAKPDCIVMHPGPINRGVEIESAVADGPHSVILNQVTYGIAIRMAVLSMAMSG
QTAQRQFEQENAQ

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=32.475884244373, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=34.6153846153846, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1786469, Length=234, Percent_Identity=29.9145299145299, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790703, Length=199, Percent_Identity=31.6582914572864, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=342, Percent_Identity=31.2865497076023, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322331, Length=317, Percent_Identity=32.4921135646688, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=33.968253968254, Blast_Score=147, Evalue=7e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 36508; Mature: 36377

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLDTKRPLQLNDQGQLRHFLSLDGLRRELLTEILDTADSFLEVGARAVKKVPLLRGKT
CCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
VCNVFFENSTRTRTTFELAAQRLSADVITLNVSTSSASKGETLLDTLRNLEAMAADMFVV
EEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEE
RHGDSGAAHFIAEHVCPQVAIINGGDGRHAHPTQGMLDMLTIRRHKGGFENLSVAIVGDI
EECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHH
LHSRVARSNMLALKTLGCPDIRVIAPKTLLPIGIEQYGVKVYTDMTEGLKDVDVVIMLRL
HHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCHHHHCEEEEECHHCCHHHHHHHHHHHH
QRERMTGGLLPSEGEFYRLFGLTTARLAGAKPDCIVMHPGPINRGVEIESAVADGPHSVI
HHHHHCCCCCCCCCCEEEEECCHHHHHCCCCCCEEEECCCCCCCCCEEHHHHCCCCHHHH
LNQVTYGIAIRMAVLSMAMSGQTAQRQFEQENAQ
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TPLDTKRPLQLNDQGQLRHFLSLDGLRRELLTEILDTADSFLEVGARAVKKVPLLRGKT
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
VCNVFFENSTRTRTTFELAAQRLSADVITLNVSTSSASKGETLLDTLRNLEAMAADMFVV
EEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEE
RHGDSGAAHFIAEHVCPQVAIINGGDGRHAHPTQGMLDMLTIRRHKGGFENLSVAIVGDI
EECCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHH
LHSRVARSNMLALKTLGCPDIRVIAPKTLLPIGIEQYGVKVYTDMTEGLKDVDVVIMLRL
HHHHHHHCCCEEEEECCCCCEEEECCCCCCCCCHHHHCEEEEECHHCCHHHHHHHHHHHH
QRERMTGGLLPSEGEFYRLFGLTTARLAGAKPDCIVMHPGPINRGVEIESAVADGPHSVI
HHHHHCCCCCCCCCCEEEEECCHHHHHCCCCCCEEEECCCCCCCCCEEHHHHCCCCHHHH
LNQVTYGIAIRMAVLSMAMSGQTAQRQFEQENAQ
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA