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The map label for this gene is pleD [H]

Identifier: 77461389

GI number: 77461389

Start: 5811464

End: 5812792

Strand: Reverse

Name: pleD [H]

Synonym: Pfl01_5168

Alternate gene names: 77461389

Gene position: 5812792-5811464 (Counterclockwise)

Preceding gene: 77461390

Following gene: 77461388

Centisome position: 90.28

GC content: 60.8

Gene sequence:

>1329_bases
ATGCCATCTCTTTGCCATGACGGGTCGGAGCTTTTACCCTTGCCGCGTTCTTCCGCTGTACGTTTCAGTCATTTCCTGCC
GTCACTGCTGTTGTTGCTCGCGGGGTTGGCGGCTGCGTACGTCAAAGACCTCAACGTGTTCTTCACCTCGCTGTTTAACG
TGTTGCCGACATTGGTGCTGCTGTTGGGCGGTTCGTACTGTGCGGTTTACCGACGCCAGCGCGAGCTGTTTCTGATGGTC
ACGGTGTACATCGCCTACTTCCTGCTCGACACCCAGACTGACTATTACCGCGACAACGGCAAGGTGCGCGAAGATGCGGC
CGTGGTGTTCCACTTGGTCTGTCTGTTGCTGCCGTTGCTGTTCGGTCTGTTCGCCGCATGGCAGGAACGCACGCATCTGT
TTCAGGACATGATCGCGCGCTTCGCCGTGCTGCTGGCCTTTGGCAGCGTGGCGCTGGGGCTCGAGCAAAGCTACCCGCAG
GCCTTGCTGATGTGGCTTTCCGAGATCCGCTGGCCGGCGCTGCACGGCGCGTGGATGAGCCTGATCCAGTTGTCGTATCC
GGTGTTTATCTCGGCGTTCCTGCTTCTGGCCTGGCAATACTGGCGCAACCCGCGTCCGCTGCACGCGGCGCAACTGGTGG
GGTTGCTCGGGGTGTTCTGGATGCTGCCGAAAACCTTCATCCTGCCGTTCACCCTGAACATCATGTGCAGCCAGGTCATG
CTGATGATTGCGGCAGCGGTAGCGCACGAGGCGTATCAAATGGCCTTCCGCGACGAACTCACCGGTTTGCCGGGACGCCG
CGCACTCAACGAGCGTATGCAGCGTCTGGGTCGCAACTATGTGCTGGCGATGAGCGACGTCGATCATTTCAAGAAATTCA
ACGACACCCACGGCCACGACGTGGGGGATCAGGTGTTGCGTCTGGTCGCCAGCAAGCTGTCCAAAATCAGCGGCGGCGGT
AGGGCGTATCGCTACGGTGGCGAGGAATTCGCCCTGGTGTTTGCGGGCAAGACCCTCGAAGAGTGCATGCCGCACCTGGA
AGTCATCCGTGAGTCGATTGCGTCCTACAACATTCAGCTGCGTAATCAGGAAAGCCGCCCGCAAGATGATCAGCAGGGGC
GTCAGCGTCGCTCGGGTTCGGGGGCGTCCAGCGTGTCGGTCACGGTCAGCATCGGCGTGGCCGAACGCCTGGAACAACGT
ACTCCGGAAGAGGTGCTGAAATCCGCTGACCAGGCGCTCTACAGTGCCAAGGGCGCAGGCCGAAACTGCGTGATGGCGTT
CGGTCAGAACCGCCGTGGCGCGGTGCGCATGGACAGCGCCGCAGGTTGA

Upstream 100 bases:

>100_bases
CTTACTGAATTACTCAGCAACTGCCGATTTCGGCCGTTAAAGAGACAGCCTGTGATCTGGATCACATTGTGTGTGCTTAA
CTGGCCACATTGCAGGCACA

Downstream 100 bases:

>100_bases
GTGATGACGGCGCATTCAGCGTCTGGACAGTGATTGTGGGGCAGGGTGACCGGCAGTAGGTTGAAGCAATCTGCTGAAGG
AGAAGCCACCATGCCCGAGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MPSLCHDGSELLPLPRSSAVRFSHFLPSLLLLLAGLAAAYVKDLNVFFTSLFNVLPTLVLLLGGSYCAVYRRQRELFLMV
TVYIAYFLLDTQTDYYRDNGKVREDAAVVFHLVCLLLPLLFGLFAAWQERTHLFQDMIARFAVLLAFGSVALGLEQSYPQ
ALLMWLSEIRWPALHGAWMSLIQLSYPVFISAFLLLAWQYWRNPRPLHAAQLVGLLGVFWMLPKTFILPFTLNIMCSQVM
LMIAAAVAHEAYQMAFRDELTGLPGRRALNERMQRLGRNYVLAMSDVDHFKKFNDTHGHDVGDQVLRLVASKLSKISGGG
RAYRYGGEEFALVFAGKTLEECMPHLEVIRESIASYNIQLRNQESRPQDDQQGRQRRSGSGASSVSVTVSIGVAERLEQR
TPEEVLKSADQALYSAKGAGRNCVMAFGQNRRGAVRMDSAAG

Sequences:

>Translated_442_residues
MPSLCHDGSELLPLPRSSAVRFSHFLPSLLLLLAGLAAAYVKDLNVFFTSLFNVLPTLVLLLGGSYCAVYRRQRELFLMV
TVYIAYFLLDTQTDYYRDNGKVREDAAVVFHLVCLLLPLLFGLFAAWQERTHLFQDMIARFAVLLAFGSVALGLEQSYPQ
ALLMWLSEIRWPALHGAWMSLIQLSYPVFISAFLLLAWQYWRNPRPLHAAQLVGLLGVFWMLPKTFILPFTLNIMCSQVM
LMIAAAVAHEAYQMAFRDELTGLPGRRALNERMQRLGRNYVLAMSDVDHFKKFNDTHGHDVGDQVLRLVASKLSKISGGG
RAYRYGGEEFALVFAGKTLEECMPHLEVIRESIASYNIQLRNQESRPQDDQQGRQRRSGSGASSVSVTVSIGVAERLEQR
TPEEVLKSADQALYSAKGAGRNCVMAFGQNRRGAVRMDSAAG
>Mature_441_residues
PSLCHDGSELLPLPRSSAVRFSHFLPSLLLLLAGLAAAYVKDLNVFFTSLFNVLPTLVLLLGGSYCAVYRRQRELFLMVT
VYIAYFLLDTQTDYYRDNGKVREDAAVVFHLVCLLLPLLFGLFAAWQERTHLFQDMIARFAVLLAFGSVALGLEQSYPQA
LLMWLSEIRWPALHGAWMSLIQLSYPVFISAFLLLAWQYWRNPRPLHAAQLVGLLGVFWMLPKTFILPFTLNIMCSQVML
MIAAAVAHEAYQMAFRDELTGLPGRRALNERMQRLGRNYVLAMSDVDHFKKFNDTHGHDVGDQVLRLVASKLSKISGGGR
AYRYGGEEFALVFAGKTLEECMPHLEVIRESIASYNIQLRNQESRPQDDQQGRQRRSGSGASSVSVTVSIGVAERLEQRT
PEEVLKSADQALYSAKGAGRNCVMAFGQNRRGAVRMDSAAG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787056, Length=187, Percent_Identity=34.7593582887701, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1786584, Length=214, Percent_Identity=31.3084112149533, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787816, Length=198, Percent_Identity=31.8181818181818, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=35, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787262, Length=184, Percent_Identity=32.0652173913043, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI145693134, Length=175, Percent_Identity=30.2857142857143, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=172, Percent_Identity=34.3023255813954, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082007, Length=223, Percent_Identity=29.1479820627803, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787802, Length=211, Percent_Identity=28.9099526066351, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49566; Mature: 49435

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSLCHDGSELLPLPRSSAVRFSHFLPSLLLLLAGLAAAYVKDLNVFFTSLFNVLPTLVL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA