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The map label for this gene is ribE [H]

Identifier: 77461239

GI number: 77461239

Start: 5653250

End: 5653912

Strand: Reverse

Name: ribE [H]

Synonym: Pfl01_5018

Alternate gene names: 77461239

Gene position: 5653912-5653250 (Counterclockwise)

Preceding gene: 77461240

Following gene: 77461238

Centisome position: 87.82

GC content: 62.29

Gene sequence:

>663_bases
ATGTTTACCGGCATCATCGAATCCATCGGCAGTATCCGCGCACTGACCCCAAAGGGCGGTGATGTGCGGGTGCATGTCGA
AACCGGCAAGCTCGACCTGAGCGACGTCAAACTCGGCGACAGCATCGCGGTCAACGGCGTGTGCCTGACCGCCGTGGAAC
TGCCGGGCAACGGCTTCGCCGCCGACGTCAGTCGCGAAACCCTCGACTGCACCGCCATGAATGACCTGAAAAGCGGCAGC
CCGGTCAACCTGGAAAAAGCCCTGACCCCGACCACCCGTCTCGGCGGGCATCTGGTCAGCGGTCACGTCGACGGTGTCGG
CGAAGTGGTTTCGCGCAGCGACAATGCCCGCGCCGTGGAATTTCGCATCCGCGCACCGAAAGAGCTGGCCAAGTACATCG
CCCACAAGGGCTCGATCACCGTTGACGGCACCAGCCTGACCGTGAACGCGGTCGATGGCGCCGAATTCCTGCTGACGATC
ATCCCGCACACCCTGAGCGAAACCATCATGGCGTCCTACAAGCCAGGTCGCCGAGTGAACCTGGAAGTCGACCTGCTGGC
GCGTTATCTGGAGCGTCTGCTTTTGGGCGACAAGGCTGCCGAGCCATCGTCCGGTGGCATCACTGAAAGCTTTCTGGCCG
CCAACGGCTACCTCAAATCCTGA

Upstream 100 bases:

>100_bases
TTGGCGATGACTGGCGAGTCACTGCCATCCCTGTCCCATCGGCGAGCGTATAATTCCCGGCCATCGCGTTAGCGCTGGCT
TCGTTCTCAAGGAGAACCCC

Downstream 100 bases:

>100_bases
CTGAAGGGGGTGCCTTGTGGCGCTCAATAGCATCGAAGAACTGGTTGAAGACATCCGCCAAGGCAAGATGGTCATTCTCA
TGGATGACGAAGACCGCGAG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MFTGIIESIGSIRALTPKGGDVRVHVETGKLDLSDVKLGDSIAVNGVCLTAVELPGNGFAADVSRETLDCTAMNDLKSGS
PVNLEKALTPTTRLGGHLVSGHVDGVGEVVSRSDNARAVEFRIRAPKELAKYIAHKGSITVDGTSLTVNAVDGAEFLLTI
IPHTLSETIMASYKPGRRVNLEVDLLARYLERLLLGDKAAEPSSGGITESFLAANGYLKS

Sequences:

>Translated_220_residues
MFTGIIESIGSIRALTPKGGDVRVHVETGKLDLSDVKLGDSIAVNGVCLTAVELPGNGFAADVSRETLDCTAMNDLKSGS
PVNLEKALTPTTRLGGHLVSGHVDGVGEVVSRSDNARAVEFRIRAPKELAKYIAHKGSITVDGTSLTVNAVDGAEFLLTI
IPHTLSETIMASYKPGRRVNLEVDLLARYLERLLLGDKAAEPSSGGITESFLAANGYLKS
>Mature_220_residues
MFTGIIESIGSIRALTPKGGDVRVHVETGKLDLSDVKLGDSIAVNGVCLTAVELPGNGFAADVSRETLDCTAMNDLKSGS
PVNLEKALTPTTRLGGHLVSGHVDGVGEVVSRSDNARAVEFRIRAPKELAKYIAHKGSITVDGTSLTVNAVDGAEFLLTI
IPHTLSETIMASYKPGRRVNLEVDLLARYLERLLLGDKAAEPSSGGITESFLAANGYLKS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=187, Percent_Identity=40.1069518716578, Blast_Score=127, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6319733, Length=206, Percent_Identity=39.3203883495146, Blast_Score=141, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23172; Mature: 23172

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIESIGSIRALTPKGGDVRVHVETGKLDLSDVKLGDSIAVNGVCLTAVELPGNGFA
CCCHHHHHHCCEEEECCCCCCEEEEEECCCEEECCEECCCCEEECCEEEEEEEECCCCCE
ADVSRETLDCTAMNDLKSGSPVNLEKALTPTTRLGGHLVSGHVDGVGEVVSRSDNARAVE
ECCCCCEEEEEEHHHCCCCCCCCHHHHCCCCHHCCCEEEECCCCCHHHHHCCCCCCEEEE
FRIRAPKELAKYIAHKGSITVDGTSLTVNAVDGAEFLLTIIPHTLSETIMASYKPGRRVN
EEECCHHHHHHHHHCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
LEVDLLARYLERLLLGDKAAEPSSGGITESFLAANGYLKS
EEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MFTGIIESIGSIRALTPKGGDVRVHVETGKLDLSDVKLGDSIAVNGVCLTAVELPGNGFA
CCCHHHHHHCCEEEECCCCCCEEEEEECCCEEECCEECCCCEEECCEEEEEEEECCCCCE
ADVSRETLDCTAMNDLKSGSPVNLEKALTPTTRLGGHLVSGHVDGVGEVVSRSDNARAVE
ECCCCCEEEEEEHHHCCCCCCCCHHHHCCCCHHCCCEEEECCCCCHHHHHCCCCCCEEEE
FRIRAPKELAKYIAHKGSITVDGTSLTVNAVDGAEFLLTIIPHTLSETIMASYKPGRRVN
EEECCHHHHHHHHHCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
LEVDLLARYLERLLLGDKAAEPSSGGITESFLAANGYLKS
EEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]