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The map label for this gene is lipA [H]

Identifier: 77461184

GI number: 77461184

Start: 5591551

End: 5592573

Strand: Reverse

Name: lipA [H]

Synonym: Pfl01_4963

Alternate gene names: 77461184

Gene position: 5592573-5591551 (Counterclockwise)

Preceding gene: 77461185

Following gene: 77461183

Centisome position: 86.86

GC content: 62.37

Gene sequence:

>1023_bases
ATGACTACTGATGCAGTGCAAACCATGATCCCGACGCTCGACGTGACCGAGCGCCCGGCCCCGCGTCCCAAGGTTGAAGC
CGGCGTCAAGCTGCGCGGCGCCGAGAAGGTTGCACGCATCCCGGTGAAGATCATTCCGACCACCGAACTGCCGAAGAAAC
CCGACTGGATCCGCGTGCGCATCCCGGTTTCGCCGGAAGTCGACCGCATCAAGAGCCTGCTGCGCAAGCACAAGCTGCAC
AGCGTCTGCGAAGAGGCGTCCTGCCCGAACCTGGGCGAGTGCTTCTCCGGCGGCACCGCGACCTTCATGATCATGGGTGA
CATCTGCACCCGTCGTTGCCCGTTCTGCGACGTTGGCCACGGTCGTCCGAAACCACTGGACGTCAATGAGCCGGAAAGCC
TGGCCATCGCCATCGCCGACCTGAAACTCAAGTACGTGGTGATCACCTCGGTTGACCGCGACGACCTGCGTGACGGCGGT
GCCCAGCACTTTGCCGACTGCATCCGCGAAATCCGCAAACTGTCGCCGAACGTGCAGCTCGAGACCCTGGTCCCGGACTA
TCGTGGCCGCATGGACATCGCCCTGGAAATCACCGCGGCCGAGCCGCCGGACGTGTTCAACCACAACCTGGAAACCGTAC
CGCGCCTGTACAAGGCTGCGCGTCCGGGTTCGGACTACCAGTGGTCGCTGACTCTGTTGCAACGCTTCAAGCAGATGATG
CCGCACATCCCGACCAAATCCGGCCTGATGCTGGGTCTGGGCGAGACCGACGACGAAGTCATCGAAGTCATGAAGCGCAT
GCGCGAACACGATATCGACATGCTGACCCTGGGCCAGTACCTGCAACCGTCCCGCAGCCACTTGCCGGTGCAGCGTTTCG
TGCACCCGGACACCTTCGCCTGGTTCGCCGAGGAAGGTTACAAGATGGGCTTCAAGAACGTCGCGTCCGGCCCATTGGTG
CGTTCTTCGTACCACGCTGACGAGCAAGCGAAACTGGTCAAGGCCGAGCTGCTCGGTTCCTGA

Upstream 100 bases:

>100_bases
TCGATTGAATTTGCCGAGGTAAGTGCCCGGCTGCGCGCGCAGCTCGTCAAACACCTCGACTATGCTGAGCAGACGACCCT
GACGGGCGGAATCGACTGAT

Downstream 100 bases:

>100_bases
TTCGGCGCTGAATGTAAAATGCCCGCCGTGGTTTATCGCCCGGCGGGCATTTTTTTGCCCGGCCTACACGCGTTACTGAC
TTTTCCTGCAATCTGCGGCG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTTDAVQTMIPTLDVTERPAPRPKVEAGVKLRGAEKVARIPVKIIPTTELPKKPDWIRVRIPVSPEVDRIKSLLRKHKLH
SVCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVNEPESLAIAIADLKLKYVVITSVDRDDLRDGG
AQHFADCIREIRKLSPNVQLETLVPDYRGRMDIALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMM
PHIPTKSGLMLGLGETDDEVIEVMKRMREHDIDMLTLGQYLQPSRSHLPVQRFVHPDTFAWFAEEGYKMGFKNVASGPLV
RSSYHADEQAKLVKAELLGS

Sequences:

>Translated_340_residues
MTTDAVQTMIPTLDVTERPAPRPKVEAGVKLRGAEKVARIPVKIIPTTELPKKPDWIRVRIPVSPEVDRIKSLLRKHKLH
SVCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVNEPESLAIAIADLKLKYVVITSVDRDDLRDGG
AQHFADCIREIRKLSPNVQLETLVPDYRGRMDIALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMM
PHIPTKSGLMLGLGETDDEVIEVMKRMREHDIDMLTLGQYLQPSRSHLPVQRFVHPDTFAWFAEEGYKMGFKNVASGPLV
RSSYHADEQAKLVKAELLGS
>Mature_339_residues
TTDAVQTMIPTLDVTERPAPRPKVEAGVKLRGAEKVARIPVKIIPTTELPKKPDWIRVRIPVSPEVDRIKSLLRKHKLHS
VCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGHGRPKPLDVNEPESLAIAIADLKLKYVVITSVDRDDLRDGGA
QHFADCIREIRKLSPNVQLETLVPDYRGRMDIALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMMP
HIPTKSGLMLGLGETDDEVIEVMKRMREHDIDMLTLGQYLQPSRSHLPVQRFVHPDTFAWFAEEGYKMGFKNVASGPLVR
SSYHADEQAKLVKAELLGS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=47.5177304964539, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI37577164, Length=245, Percent_Identity=47.7551020408163, Blast_Score=237, Evalue=1e-62,
Organism=Escherichia coli, GI1786846, Length=310, Percent_Identity=65.4838709677419, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32564533, Length=331, Percent_Identity=41.3897280966767, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=289, Percent_Identity=44.636678200692, Blast_Score=249, Evalue=3e-67,
Organism=Drosophila melanogaster, GI221513272, Length=282, Percent_Identity=48.936170212766, Blast_Score=272, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 38186; Mature: 38055

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDAVQTMIPTLDVTERPAPRPKVEAGVKLRGAEKVARIPVKIIPTTELPKKPDWIRVR
CCCHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCEEEECCCCCCCCCCEEEEE
IPVSPEVDRIKSLLRKHKLHSVCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGH
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECHHHCCCCCCCCCC
GRPKPLDVNEPESLAIAIADLKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLSPNVQL
CCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEE
ETLVPDYRGRMDIALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMM
EEECCCCCCCEEEEEEEECCCCCHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
PHIPTKSGLMLGLGETDDEVIEVMKRMREHDIDMLTLGQYLQPSRSHLPVQRFVHPDTFA
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHHCCCCHHH
WFAEEGYKMGFKNVASGPLVRSSYHADEQAKLVKAELLGS
HHHHHCHHCCHHHHCCCCCEECCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TTDAVQTMIPTLDVTERPAPRPKVEAGVKLRGAEKVARIPVKIIPTTELPKKPDWIRVR
CCHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHCCEEEECCCCCCCCCCEEEEE
IPVSPEVDRIKSLLRKHKLHSVCEEASCPNLGECFSGGTATFMIMGDICTRRCPFCDVGH
ECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEEECHHHCCCCCCCCCC
GRPKPLDVNEPESLAIAIADLKLKYVVITSVDRDDLRDGGAQHFADCIREIRKLSPNVQL
CCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCHHHHHCCCHHHHHHHHHHHHHCCCCCEE
ETLVPDYRGRMDIALEITAAEPPDVFNHNLETVPRLYKAARPGSDYQWSLTLLQRFKQMM
EEECCCCCCCEEEEEEEECCCCCHHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
PHIPTKSGLMLGLGETDDEVIEVMKRMREHDIDMLTLGQYLQPSRSHLPVQRFVHPDTFA
CCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHHCCCCHHH
WFAEEGYKMGFKNVASGPLVRSSYHADEQAKLVKAELLGS
HHHHHCHHCCHHHHCCCCCEECCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA