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The map label for this gene is ykfA [H]

Identifier: 77461183

GI number: 77461183

Start: 5590430

End: 5591377

Strand: Reverse

Name: ykfA [H]

Synonym: Pfl01_4962

Alternate gene names: 77461183

Gene position: 5591377-5590430 (Counterclockwise)

Preceding gene: 77461184

Following gene: 77461177

Centisome position: 86.84

GC content: 62.45

Gene sequence:

>948_bases
ATGACCGTTCGACCGACCCACACCCTTTTCCCGCACAAGCCTGTGCCCGCGCTGCCGTCGGAAGGGCTGATAGGCGTCAT
TGCGCCTGCCGGCCCCGGCACGCTGGACACTGAAAAGGCCGTTCAGTGGATGCGCGCCCGGGGCTACGGTTTGCGAGTGT
TCCCTGGCGTCTACGAGAAGGAGGGGTATCTGGCCGGCAGTGACGAGGTGCGGCTCAACGATCTGCACGCGGCGTTCGCT
GACGAAGAGGTCGATGCGATCATTTGCCTGCGCGGTGGTTACGGTACGCCAAGGTTGCTGGATCGCATCGATTACGACCT
GCTGAGCCGCCATGCCAAGCCCTTCGTCGGTTACAGCGACATCACCGCACTGCATCTGGCCATCAGCCGTTATGCCGGTT
TCGTGACCTTTCACGGGCCGCTGCTGAATGCCGATCTGCTGGGGGGCAAGGAACCGCCGACGGTGACGTCATTCTTCGCC
ATGCTGCGGGGTCAGTTGAAGGCCGGCAGTGTGCTGATTCATCCGGCGGCTTATCCATTGACCACCGTGGAACCCGGCAT
TGCCCACGGGCGTTTGCAGGGAGGCAATCTGGCGATGATCGCCGCGACCCTGGGCACGCCCTACGAAATCGACGTCGAAG
GGGTGATTCTGCTCATCGAGGACATCAACGAGCCGCTGTACCGGATCGACCGTTTGCTGACCCAGATGCGACTGGCGGGC
AAGCTGGCCAGATTGCGCGGCGTGCTGGTTGGGGATGTGGCGGGCGTGGATGTCGAGGCCTTGAACCGCTTGCTCAAGCA
GACTTTTGAACCGCTGCGGATTCCGGTGCTGTCCGGCTGGCGCAGCGGGCACTGCGATCCGAACCTGACGCTGCCGCTGG
GGGCGCTGGTTCGACTGGATGCAGGGAAGAAAGAATTGATGCTGGAACAGGATGTAGTGATTCGCTGA

Upstream 100 bases:

>100_bases
TACTGACTTTTCCTGCAATCTGCGGCGCGGCCGAGCCTCTTCTGTTTGTCGCCGTTCCTGACTACTGTGTGAGCATGACT
TCACACAGGGAGATCCATGG

Downstream 100 bases:

>100_bases
TCGAGCCACAAAAAAAGACCGCCGCCTGAAGAGGCGGCGGTCTTTTGTCTTTACTGGTTACGCAGGCTTTCCAGCAGTTT
GTGCGTCGGATAACCGTCCG

Product: peptidase U61, LD-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTVRPTHTLFPHKPVPALPSEGLIGVIAPAGPGTLDTEKAVQWMRARGYGLRVFPGVYEKEGYLAGSDEVRLNDLHAAFA
DEEVDAIICLRGGYGTPRLLDRIDYDLLSRHAKPFVGYSDITALHLAISRYAGFVTFHGPLLNADLLGGKEPPTVTSFFA
MLRGQLKAGSVLIHPAAYPLTTVEPGIAHGRLQGGNLAMIAATLGTPYEIDVEGVILLIEDINEPLYRIDRLLTQMRLAG
KLARLRGVLVGDVAGVDVEALNRLLKQTFEPLRIPVLSGWRSGHCDPNLTLPLGALVRLDAGKKELMLEQDVVIR

Sequences:

>Translated_315_residues
MTVRPTHTLFPHKPVPALPSEGLIGVIAPAGPGTLDTEKAVQWMRARGYGLRVFPGVYEKEGYLAGSDEVRLNDLHAAFA
DEEVDAIICLRGGYGTPRLLDRIDYDLLSRHAKPFVGYSDITALHLAISRYAGFVTFHGPLLNADLLGGKEPPTVTSFFA
MLRGQLKAGSVLIHPAAYPLTTVEPGIAHGRLQGGNLAMIAATLGTPYEIDVEGVILLIEDINEPLYRIDRLLTQMRLAG
KLARLRGVLVGDVAGVDVEALNRLLKQTFEPLRIPVLSGWRSGHCDPNLTLPLGALVRLDAGKKELMLEQDVVIR
>Mature_314_residues
TVRPTHTLFPHKPVPALPSEGLIGVIAPAGPGTLDTEKAVQWMRARGYGLRVFPGVYEKEGYLAGSDEVRLNDLHAAFAD
EEVDAIICLRGGYGTPRLLDRIDYDLLSRHAKPFVGYSDITALHLAISRYAGFVTFHGPLLNADLLGGKEPPTVTSFFAM
LRGQLKAGSVLIHPAAYPLTTVEPGIAHGRLQGGNLAMIAATLGTPYEIDVEGVILLIEDINEPLYRIDRLLTQMRLAGK
LARLRGVLVGDVAGVDVEALNRLLKQTFEPLRIPVLSGWRSGHCDPNLTLPLGALVRLDAGKKELMLEQDVVIR

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=300, Percent_Identity=30.3333333333333, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 34217; Mature: 34086

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRPTHTLFPHKPVPALPSEGLIGVIAPAGPGTLDTEKAVQWMRARGYGLRVFPGVYEK
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEECCCCCCC
EGYLAGSDEVRLNDLHAAFADEEVDAIICLRGGYGTPRLLDRIDYDLLSRHAKPFVGYSD
CCEECCCCCEEEEHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCHHHHHHCCCCCCCHHH
ITALHLAISRYAGFVTFHGPLLNADLLGGKEPPTVTSFFAMLRGQLKAGSVLIHPAAYPL
HHHHHHHHHHHCCEEEECCCEECHHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCC
TTVEPGIAHGRLQGGNLAMIAATLGTPYEIDVEGVILLIEDINEPLYRIDRLLTQMRLAG
CCCCCCCCCCEECCCCEEEEEEECCCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHH
KLARLRGVLVGDVAGVDVEALNRLLKQTFEPLRIPVLSGWRSGHCDPNLTLPLGALVRLD
HHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEECCCEEEEEC
AGKKELMLEQDVVIR
CCCHHHEECCCCCCC
>Mature Secondary Structure 
TVRPTHTLFPHKPVPALPSEGLIGVIAPAGPGTLDTEKAVQWMRARGYGLRVFPGVYEK
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEECCCCCCC
EGYLAGSDEVRLNDLHAAFADEEVDAIICLRGGYGTPRLLDRIDYDLLSRHAKPFVGYSD
CCEECCCCCEEEEHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCHHHHHHCCCCCCCHHH
ITALHLAISRYAGFVTFHGPLLNADLLGGKEPPTVTSFFAMLRGQLKAGSVLIHPAAYPL
HHHHHHHHHHHCCEEEECCCEECHHHCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCC
TTVEPGIAHGRLQGGNLAMIAATLGTPYEIDVEGVILLIEDINEPLYRIDRLLTQMRLAG
CCCCCCCCCCEECCCCEEEEEEECCCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHH
KLARLRGVLVGDVAGVDVEALNRLLKQTFEPLRIPVLSGWRSGHCDPNLTLPLGALVRLD
HHHHHHCEEEECCCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEECCCEEEEEC
AGKKELMLEQDVVIR
CCCHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]