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The map label for this gene is lipB [H]

Identifier: 77461185

GI number: 77461185

Start: 5592575

End: 5593222

Strand: Reverse

Name: lipB [H]

Synonym: Pfl01_4964

Alternate gene names: 77461185

Gene position: 5593222-5592575 (Counterclockwise)

Preceding gene: 77461186

Following gene: 77461184

Centisome position: 86.87

GC content: 63.58

Gene sequence:

>648_bases
ATGCCGGGCACGCTGGGCTTTCGCGAGCTTGGCCAGATGGCTTACGAGCCGGTCTGGCAGGCCATGCAACGTTTTACCAA
CGAGCGTGGCACCACGGCCCCGGACGAAATCTGGCTGGTCGAACACCCGCCGGTGTTTACCCAGGGCCAGGCCGGCAAGG
CCGAGCACCTGTTGCTGCCGGGAGACATTCCGGTGGTGCAGGTCGACCGCGGCGGTCAGGTGACCTACCATGGCCCAGGC
CAGTTGGTAGCCTATCTGCTGCTGGATGTGCGCCGACTCGGTTTCGGCGTGCGCGATCTGGTCAGCCGCATGGAGCGCTG
CCTGATCGAACTGCTGGCCAGCTACGGCGTGACCGCCGTGGCCAAGCCGGATGCTCCCGGCGTCTACATCGACGGAGCGA
AGATCGCTTCTCTGGGTTTGCGGATTCGCCACGGTTGTTCCTTTCATGGCCTGGCCCTGAACGTGGACATGAACCTGGAA
CCGTTTCGACGGATTAATCCCTGCGGCTATGCCGGGCTGGCGATGACCCAGCTGAGCGAGCACGCAGGATCGATTGAATT
TGCCGAGGTAAGTGCCCGGCTGCGCGCGCAGCTCGTCAAACACCTCGACTATGCTGAGCAGACGACCCTGACGGGCGGAA
TCGACTGA

Upstream 100 bases:

>100_bases
ACACCACCATCCAGTTGCACATCGTCGCGACCGATCAGGATCAGCTGTACAACATCAACAGCGAACTGCGGGCTACCGGT
TTCGTGCACATGGTGCTGTG

Downstream 100 bases:

>100_bases
TATGACTACTGATGCAGTGCAAACCATGATCCCGACGCTCGACGTGACCGAGCGCCCGGCCCCGCGTCCCAAGGTTGAAG
CCGGCGTCAAGCTGCGCGGC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MPGTLGFRELGQMAYEPVWQAMQRFTNERGTTAPDEIWLVEHPPVFTQGQAGKAEHLLLPGDIPVVQVDRGGQVTYHGPG
QLVAYLLLDVRRLGFGVRDLVSRMERCLIELLASYGVTAVAKPDAPGVYIDGAKIASLGLRIRHGCSFHGLALNVDMNLE
PFRRINPCGYAGLAMTQLSEHAGSIEFAEVSARLRAQLVKHLDYAEQTTLTGGID

Sequences:

>Translated_215_residues
MPGTLGFRELGQMAYEPVWQAMQRFTNERGTTAPDEIWLVEHPPVFTQGQAGKAEHLLLPGDIPVVQVDRGGQVTYHGPG
QLVAYLLLDVRRLGFGVRDLVSRMERCLIELLASYGVTAVAKPDAPGVYIDGAKIASLGLRIRHGCSFHGLALNVDMNLE
PFRRINPCGYAGLAMTQLSEHAGSIEFAEVSARLRAQLVKHLDYAEQTTLTGGID
>Mature_214_residues
PGTLGFRELGQMAYEPVWQAMQRFTNERGTTAPDEIWLVEHPPVFTQGQAGKAEHLLLPGDIPVVQVDRGGQVTYHGPGQ
LVAYLLLDVRRLGFGVRDLVSRMERCLIELLASYGVTAVAKPDAPGVYIDGAKIASLGLRIRHGCSFHGLALNVDMNLEP
FRRINPCGYAGLAMTQLSEHAGSIEFAEVSARLRAQLVKHLDYAEQTTLTGGID

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=113, Percent_Identity=42.4778761061947, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI87081767, Length=205, Percent_Identity=55.609756097561, Blast_Score=240, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6323268, Length=180, Percent_Identity=30, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355483, Length=180, Percent_Identity=30, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 23494; Mature: 23363

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTLGFRELGQMAYEPVWQAMQRFTNERGTTAPDEIWLVEHPPVFTQGQAGKAEHLLLP
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEC
GDIPVVQVDRGGQVTYHGPGQLVAYLLLDVRRLGFGVRDLVSRMERCLIELLASYGVTAV
CCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEE
AKPDAPGVYIDGAKIASLGLRIRHGCSFHGLALNVDMNLEPFRRINPCGYAGLAMTQLSE
ECCCCCCEEECCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCHHHHHHHHHH
HAGSIEFAEVSARLRAQLVKHLDYAEQTTLTGGID
HCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
>Mature Secondary Structure 
PGTLGFRELGQMAYEPVWQAMQRFTNERGTTAPDEIWLVEHPPVFTQGQAGKAEHLLLP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEC
GDIPVVQVDRGGQVTYHGPGQLVAYLLLDVRRLGFGVRDLVSRMERCLIELLASYGVTAV
CCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEE
AKPDAPGVYIDGAKIASLGLRIRHGCSFHGLALNVDMNLEPFRRINPCGYAGLAMTQLSE
ECCCCCCEEECCHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCHHHHHHHHHH
HAGSIEFAEVSARLRAQLVKHLDYAEQTTLTGGID
HCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA