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The map label for this gene is pilD [H]

Identifier: 77461043

GI number: 77461043

Start: 5442636

End: 5443508

Strand: Direct

Name: pilD [H]

Synonym: Pfl01_4822

Alternate gene names: 77461043

Gene position: 5442636-5443508 (Clockwise)

Preceding gene: 77461042

Following gene: 77461044

Centisome position: 84.53

GC content: 61.4

Gene sequence:

>873_bases
ATGCCTATCGACGAACTCTTCAGCCTGTATCCGCTGGCCTTTGTCTTCACCGCCCTGCTGCTGGGGCTGGTGGTCGGCAG
TTTCCTCAACGTGTTGATCTGGCGCCTGCCGAAAATGCTCGAGCGTGAATGGCGCCAGCAGGCTCACGATGTTTTGGGGC
TGCCCGGTGAAGCACCGTTACCCACTTACAACCTGATGCTGCCGCACTCCCAATGCCCGCACTGTGGCCACCGGATCCGG
GCCTGGGAAAACATTCCCGTGTTGAGTTACGTGTTTCTGCGCGGGCGCTGTTCAAGTTGCAGCGCACCGATCAGCAAGCG
TTACCCGCTGACCGAACTGGCCTGCGGCCTGCTCTCGGCGTTCATCGCCTGGCACCTGGGTTTCGGCTGGCCGGCGTGCC
TGCTGATCGTGCTGACCTGGGGCCTGTTGGCCATGAGCCTGATCGACACCGAACATCAATTGTTGCCCGACGTGCTGGTG
CTGCCGCTGCTGTGGCTGGGGCTGATCGTCAACAGCTTCGAGCTGTTCGTGCCGCTGCACGATGCCCTGTGGGGCGCGGT
GCTTGGGTACATGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAACTGATCACCGGCAAGGACGGCATCGGTCACGGCGATT
TCAAGCTGCTGGCGCTGCTCGGGGCCTGGGGCGGCTGGCAGATTCTGCCGCTGACCATCCTGCTGTCTTCGCTGGTGGGC
GCCGTGCTCGGGGTGATTTTGCTCAAGCTGCGCGACCAGAAAACCTCGACGCCGATTCCCTTCGGCCCGTATCTGGCAAT
TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTTCTATTGGCAGTTTGTCGGTTTGAAATGA

Upstream 100 bases:

>100_bases
CTGATGGAGCCGTTCATCATGGTGGTGCTCGGGGTGATCGTCGGCGGTCTGGTGGTGGCGATGTACCTGCCGATCTTCCA
ACTCGGCTCAGCGATCTGAC

Downstream 100 bases:

>100_bases
ATACCCCTGTGGAAAAACCCTGGATTCTCGGCCTGACCGGCGGCATCGGCAGCGGCAAAAGCGCCGCCGCCCAGCACTTC
ATCGATCTTGGTATCCACGT

Product: type 4 prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MPIDELFSLYPLAFVFTALLLGLVVGSFLNVLIWRLPKMLEREWRQQAHDVLGLPGEAPLPTYNLMLPHSQCPHCGHRIR
AWENIPVLSYVFLRGRCSSCSAPISKRYPLTELACGLLSAFIAWHLGFGWPACLLIVLTWGLLAMSLIDTEHQLLPDVLV
LPLLWLGLIVNSFELFVPLHDALWGAVLGYMALWSVFWLFKLITGKDGIGHGDFKLLALLGAWGGWQILPLTILLSSLVG
AVLGVILLKLRDQKTSTPIPFGPYLAIAGWIALLWGGQITGFYWQFVGLK

Sequences:

>Translated_290_residues
MPIDELFSLYPLAFVFTALLLGLVVGSFLNVLIWRLPKMLEREWRQQAHDVLGLPGEAPLPTYNLMLPHSQCPHCGHRIR
AWENIPVLSYVFLRGRCSSCSAPISKRYPLTELACGLLSAFIAWHLGFGWPACLLIVLTWGLLAMSLIDTEHQLLPDVLV
LPLLWLGLIVNSFELFVPLHDALWGAVLGYMALWSVFWLFKLITGKDGIGHGDFKLLALLGAWGGWQILPLTILLSSLVG
AVLGVILLKLRDQKTSTPIPFGPYLAIAGWIALLWGGQITGFYWQFVGLK
>Mature_289_residues
PIDELFSLYPLAFVFTALLLGLVVGSFLNVLIWRLPKMLEREWRQQAHDVLGLPGEAPLPTYNLMLPHSQCPHCGHRIRA
WENIPVLSYVFLRGRCSSCSAPISKRYPLTELACGLLSAFIAWHLGFGWPACLLIVLTWGLLAMSLIDTEHQLLPDVLVL
PLLWLGLIVNSFELFVPLHDALWGAVLGYMALWSVFWLFKLITGKDGIGHGDFKLLALLGAWGGWQILPLTILLSSLVGA
VLGVILLKLRDQKTSTPIPFGPYLAIAGWIALLWGGQITGFYWQFVGLK

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=268, Percent_Identity=40.2985074626866, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=50.7575757575758, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32305; Mature: 32174

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHEECCC
>Mature Secondary Structure 
PIDELFSLYPLAFVFTALLLGLVVGSFLNVLIWRLPKMLEREWRQQAHDVLGLPGEAPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PTYNLMLPHSQCPHCGHRIRAWENIPVLSYVFLRGRCSSCSAPISKRYPLTELACGLLSA
CCEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
FIAWHLGFGWPACLLIVLTWGLLAMSLIDTEHQLLPDVLVLPLLWLGLIVNSFELFVPLH
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DALWGAVLGYMALWSVFWLFKLITGKDGIGHGDFKLLALLGAWGGWQILPLTILLSSLVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AVLGVILLKLRDQKTSTPIPFGPYLAIAGWIALLWGGQITGFYWQFVGLK
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA