Click here to switch to the map view.

The map label for this gene is dctD [H]

Identifier: 77460761

GI number: 77460761

Start: 5115368

End: 5116696

Strand: Direct

Name: dctD [H]

Synonym: Pfl01_4540

Alternate gene names: 77460761

Gene position: 5115368-5116696 (Clockwise)

Preceding gene: 77460760

Following gene: 77460762

Centisome position: 79.45

GC content: 63.58

Gene sequence:

>1329_bases
ATGAACAACGACCTCAGTGTATTGATCGTCGAAGACGACCCCCATGTCCTGCTCGGCTGCCAGCAGGCGCTGACCCTCGA
AGACATCCCCTGCATCGGCGTCGGCAGTGGCGAAGAAGCGCTGGAGCAGGTCGGCGACAACTTCGCCGGGATCGTCATCA
GCGACATTCGCCTGCCGGGCATCGACGGCCTGGAACTGCTGACCCGGCTCAAGCAACGCGACCGCAGCCTGCCGGTGGTG
CTGATCACCGGCCACGGCGACATCTCCATGGCCGTCGGCGCGATGCAGAAAGGCGCCTACGACTTCATGGAAAAACCGTT
CTCCCCTGAACGACTGGTGGACGTGGCCCGTCGTGCGCTGGAGCAACGCAGCCTCAATCGCGAAGTTTCGTCCCTGCGCC
GGCAACTGGCCGAACGCGATTCCCTTGAAGGGCGGATCATCGGGCGTTCGCCGGCCATGCAGAACCTGCGCGAACTGATC
GCCAACGTCGCCGACACCTCGGCCAACGTATTGATCGAAGGCGAGACCGGCACCGGCAAGGAACTGGTCGCCCGCTGCCT
GCACGATTTCAGCCGACGCCACACCAAACAGTTCGTCGCGCTGAACTGCGGCGGTCTGCCGGAAAACCTGTTCGAAAGCG
AAATCTTCGGTCACGAAGCCAACGCCTTCACCGGCGCCGGCAAACGGCGGATCGGCAAGATCGAGCACGCCGACGGCGGC
ACGCTGTTCCTCGACGAAGTGGAAAGCATGCCGCTGCCGTTGCAGATCAAACTGCTGCGGGTGTTGCAGGAGCGCACCCT
CGAACGCCTCGGCTCGAACCAGAGCGTGGCGGTGGATTGCCGGGTGATTGCGGCCACCAAGTCCGACCTCGACGAGTCGA
GCAAGGCCGGGGAATTTCGCAGTGACTTGTACTACCGCCTCAACGTGGTGACGCTGGAACTGCCGCCCCTGCGCGAACGC
CGCGAAGACATCCTGCAACTGTTCGAACACTTCACCCAGCAATCGGCCCTGCGTTTCGACCGGGCGCTGCCGGAGCTGGA
CAACCAGACGTTGTCGAGCCTGATGAGCCACGACTGGCCGGGCAACGTGCGCGAACTGCGCAACGTCGCCGAGCGTTTTG
CCCTCGGTTTGCCGGCGTTCAAGAAAACCGGCGCGGGCAGCGCAGGTCAGGGCCTGGCGTTCGCCGAAGCGGTGGAAGCG
TTCGAACGCAACCTGCTCAGCGACGCCCTGCAACGCAGCGGCGGCAACCTGACCCAGGCCAGCCTGGAACTGGGCATGGC
CAAGACCACGCTGTTCGACAAAGTGAAAAAATACGGCCTGAGCCATTAA

Upstream 100 bases:

>100_bases
CCGCCACCGGCGGGCATCTGGGTGTCGAGCACCCGGCCAGCGGTGGTACCACTTTCGTCCTCAGTTTACCGTTGGTAAGC
CCTACTCCTGCCGAGCCAAT

Downstream 100 bases:

>100_bases
GCGAGAACAACGTGGATCTGATTTTCAAGGCAATGCTCGGTGCGGCCGTGGTGGTGATCCTCGCCATGCTGGCCAAGACC
AAAAACTACTACATTGCCGG

Product: two component, sigma-54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MNNDLSVLIVEDDPHVLLGCQQALTLEDIPCIGVGSGEEALEQVGDNFAGIVISDIRLPGIDGLELLTRLKQRDRSLPVV
LITGHGDISMAVGAMQKGAYDFMEKPFSPERLVDVARRALEQRSLNREVSSLRRQLAERDSLEGRIIGRSPAMQNLRELI
ANVADTSANVLIEGETGTGKELVARCLHDFSRRHTKQFVALNCGGLPENLFESEIFGHEANAFTGAGKRRIGKIEHADGG
TLFLDEVESMPLPLQIKLLRVLQERTLERLGSNQSVAVDCRVIAATKSDLDESSKAGEFRSDLYYRLNVVTLELPPLRER
REDILQLFEHFTQQSALRFDRALPELDNQTLSSLMSHDWPGNVRELRNVAERFALGLPAFKKTGAGSAGQGLAFAEAVEA
FERNLLSDALQRSGGNLTQASLELGMAKTTLFDKVKKYGLSH

Sequences:

>Translated_442_residues
MNNDLSVLIVEDDPHVLLGCQQALTLEDIPCIGVGSGEEALEQVGDNFAGIVISDIRLPGIDGLELLTRLKQRDRSLPVV
LITGHGDISMAVGAMQKGAYDFMEKPFSPERLVDVARRALEQRSLNREVSSLRRQLAERDSLEGRIIGRSPAMQNLRELI
ANVADTSANVLIEGETGTGKELVARCLHDFSRRHTKQFVALNCGGLPENLFESEIFGHEANAFTGAGKRRIGKIEHADGG
TLFLDEVESMPLPLQIKLLRVLQERTLERLGSNQSVAVDCRVIAATKSDLDESSKAGEFRSDLYYRLNVVTLELPPLRER
REDILQLFEHFTQQSALRFDRALPELDNQTLSSLMSHDWPGNVRELRNVAERFALGLPAFKKTGAGSAGQGLAFAEAVEA
FERNLLSDALQRSGGNLTQASLELGMAKTTLFDKVKKYGLSH
>Mature_442_residues
MNNDLSVLIVEDDPHVLLGCQQALTLEDIPCIGVGSGEEALEQVGDNFAGIVISDIRLPGIDGLELLTRLKQRDRSLPVV
LITGHGDISMAVGAMQKGAYDFMEKPFSPERLVDVARRALEQRSLNREVSSLRRQLAERDSLEGRIIGRSPAMQNLRELI
ANVADTSANVLIEGETGTGKELVARCLHDFSRRHTKQFVALNCGGLPENLFESEIFGHEANAFTGAGKRRIGKIEHADGG
TLFLDEVESMPLPLQIKLLRVLQERTLERLGSNQSVAVDCRVIAATKSDLDESSKAGEFRSDLYYRLNVVTLELPPLRER
REDILQLFEHFTQQSALRFDRALPELDNQTLSSLMSHDWPGNVRELRNVAERFALGLPAFKKTGAGSAGQGLAFAEAVEA
FERNLLSDALQRSGGNLTQASLELGMAKTTLFDKVKKYGLSH

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=451, Percent_Identity=39.9113082039911, Blast_Score=331, Evalue=7e-92,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.8888888888889, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790437, Length=447, Percent_Identity=40.2684563758389, Blast_Score=314, Evalue=6e-87,
Organism=Escherichia coli, GI1788905, Length=442, Percent_Identity=39.8190045248869, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=44.6064139941691, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=45.6521739130435, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1786524, Length=343, Percent_Identity=41.6909620991254, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=38.4375, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI87082152, Length=321, Percent_Identity=41.1214953271028, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI87081872, Length=228, Percent_Identity=45.6140350877193, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787583, Length=334, Percent_Identity=36.8263473053892, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=31.8471337579618, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=36.4372469635627, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1790552, Length=128, Percent_Identity=32.8125, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48725; Mature: 48725

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDLSVLIVEDDPHVLLGCQQALTLEDIPCIGVGSGEEALEQVGDNFAGIVISDIRLPG
CCCCEEEEEECCCCCEEEECHHHCCCCCCCEEECCCCHHHHHHHCCCCCCEEEECCCCCC
IDGLELLTRLKQRDRSLPVVLITGHGDISMAVGAMQKGAYDFMEKPFSPERLVDVARRAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
EQRSLNREVSSLRRQLAERDSLEGRIIGRSPAMQNLRELIANVADTSANVLIEGETGTGK
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCH
ELVARCLHDFSRRHTKQFVALNCGGLPENLFESEIFGHEANAFTGAGKRRIGKIEHADGG
HHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCEECCCC
TLFLDEVESMPLPLQIKLLRVLQERTLERLGSNQSVAVDCRVIAATKSDLDESSKAGEFR
EEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECHHCCCCCCCCCHHH
SDLYYRLNVVTLELPPLRERREDILQLFEHFTQQSALRFDRALPELDNQTLSSLMSHDWP
HHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
GNVRELRNVAERFALGLPAFKKTGAGSAGQGLAFAEAVEAFERNLLSDALQRSGGNLTQA
CCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
SLELGMAKTTLFDKVKKYGLSH
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNDLSVLIVEDDPHVLLGCQQALTLEDIPCIGVGSGEEALEQVGDNFAGIVISDIRLPG
CCCCEEEEEECCCCCEEEECHHHCCCCCCCEEECCCCHHHHHHHCCCCCCEEEECCCCCC
IDGLELLTRLKQRDRSLPVVLITGHGDISMAVGAMQKGAYDFMEKPFSPERLVDVARRAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHH
EQRSLNREVSSLRRQLAERDSLEGRIIGRSPAMQNLRELIANVADTSANVLIEGETGTGK
HHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCH
ELVARCLHDFSRRHTKQFVALNCGGLPENLFESEIFGHEANAFTGAGKRRIGKIEHADGG
HHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCEECCCC
TLFLDEVESMPLPLQIKLLRVLQERTLERLGSNQSVAVDCRVIAATKSDLDESSKAGEFR
EEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECHHCCCCCCCCCHHH
SDLYYRLNVVTLELPPLRERREDILQLFEHFTQQSALRFDRALPELDNQTLSSLMSHDWP
HHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
GNVRELRNVAERFALGLPAFKKTGAGSAGQGLAFAEAVEAFERNLLSDALQRSGGNLTQA
CCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
SLELGMAKTTLFDKVKKYGLSH
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]