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The map label for this gene is rfbB [C]

Identifier: 77460292

GI number: 77460292

Start: 4598460

End: 4599470

Strand: Direct

Name: rfbB [C]

Synonym: Pfl01_4071

Alternate gene names: 77460292

Gene position: 4598460-4599470 (Clockwise)

Preceding gene: 77460291

Following gene: 77460301

Centisome position: 71.42

GC content: 55.89

Gene sequence:

>1011_bases
ATGAAGATACTCGTGACTGGCGCAGCCGGTTTCATCGGCTTTCATGTCAGCCAGGCGCTGCTTGCCCGCGGCGATGAAGT
CGTTGGCATCGATAATCTGAACGACTATTACGAAGTGGCTCTCAAGGATGCTCGCCTCGCGCAATTGACGCCGCAGGAGG
GCTTCCGGTTTATCCGCATGGATATCGGCGACCGGACTGCGATGGCGGAACTGTTCGAGACAGAAGGCTTTGAAAAAGTT
GTCCATCTCGCGGCGCAAGCCGGCGTGCGGTACTCGCTGGAGAATCCTCACGCGTATGCAGACAGCAACCTTACAGGCTT
CTTGAACGTTCTCGAGGGCTGTCGACAAACTCACGTCAAACACTTGGTATATGCCTCGTCCAGTTCAGTGTATGGCGCTA
ACGAGAGCATGCCCTTCGCCGTGCAAGACAACGTTGACCATCCCGTCTCGCTGTATGCCGCCACGAAGAAAGCCAACGAA
CTGATGGCGCACTCGTACAGTCATCTCTATCGGTTGCCGACTACCGGATTGCGCTTTTTCACGGTCTACGGCCCTTGGGG
CCGGCCGGACATGTCGCCGTTCCTGTTCGTGCGGGCAATCCTTGAAGGCAAACCGCTGAAGGTTTTCAATTACGGAAAGC
ATCGCCGAGACTTCACGTACATCGACGACATCGTCGACGGCGTCATACGGGTCCTGGACCATGTGGCTGCACCGAACGCT
CAATGGTCAGGACTCAAGCCGGATCCGGCCTCCAGTGCGGCGCCCTGGAGGCTTTACAACATTGGCAACAGTCAACCGGT
CGAATTGCTGGACTACATCAAGCACATCGAACAAGCGCTCGGCAAAACCACTCATAAAGAGCTTCTGCCGTTGCAGCCGG
GCGATGTCGAACACACCTATGCCGATGTCGAGCAGTTGAAGATCGATACCGGGTATGCACCCGACACGCCGATTGAAAGC
GGGATACAGCGCTTTGTGAACTGGTACAAGGACTTCTACAACATCAGCTGA

Upstream 100 bases:

>100_bases
TGTTTGACCCTGCCGCGGCCAAGGAGGCAGGGTTCATGTACTACGCAATCGGTCGTGCTGACTCGCTCATGCCCGTTCTG
CAACCTTAAGGAGCTGTCCC

Downstream 100 bases:

>100_bases
TGCTCTTATCGTTCAGCCATCAAAAGACGCCGGATATTGTGATCCGGCGTTTTTTTTGTCGAATTCCAGGCACAAAAAAG
GGCGACCGAAGTCGCCCTTT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKILVTGAAGFIGFHVSQALLARGDEVVGIDNLNDYYEVALKDARLAQLTPQEGFRFIRMDIGDRTAMAELFETEGFEKV
VHLAAQAGVRYSLENPHAYADSNLTGFLNVLEGCRQTHVKHLVYASSSSVYGANESMPFAVQDNVDHPVSLYAATKKANE
LMAHSYSHLYRLPTTGLRFFTVYGPWGRPDMSPFLFVRAILEGKPLKVFNYGKHRRDFTYIDDIVDGVIRVLDHVAAPNA
QWSGLKPDPASSAAPWRLYNIGNSQPVELLDYIKHIEQALGKTTHKELLPLQPGDVEHTYADVEQLKIDTGYAPDTPIES
GIQRFVNWYKDFYNIS

Sequences:

>Translated_336_residues
MKILVTGAAGFIGFHVSQALLARGDEVVGIDNLNDYYEVALKDARLAQLTPQEGFRFIRMDIGDRTAMAELFETEGFEKV
VHLAAQAGVRYSLENPHAYADSNLTGFLNVLEGCRQTHVKHLVYASSSSVYGANESMPFAVQDNVDHPVSLYAATKKANE
LMAHSYSHLYRLPTTGLRFFTVYGPWGRPDMSPFLFVRAILEGKPLKVFNYGKHRRDFTYIDDIVDGVIRVLDHVAAPNA
QWSGLKPDPASSAAPWRLYNIGNSQPVELLDYIKHIEQALGKTTHKELLPLQPGDVEHTYADVEQLKIDTGYAPDTPIES
GIQRFVNWYKDFYNIS
>Mature_336_residues
MKILVTGAAGFIGFHVSQALLARGDEVVGIDNLNDYYEVALKDARLAQLTPQEGFRFIRMDIGDRTAMAELFETEGFEKV
VHLAAQAGVRYSLENPHAYADSNLTGFLNVLEGCRQTHVKHLVYASSSSVYGANESMPFAVQDNVDHPVSLYAATKKANE
LMAHSYSHLYRLPTTGLRFFTVYGPWGRPDMSPFLFVRAILEGKPLKVFNYGKHRRDFTYIDDIVDGVIRVLDHVAAPNA
QWSGLKPDPASSAAPWRLYNIGNSQPVELLDYIKHIEQALGKTTHKELLPLQPGDVEHTYADVEQLKIDTGYAPDTPIES
GIQRFVNWYKDFYNIS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=25.0728862973761, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=24.0356083086053, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI56237023, Length=345, Percent_Identity=27.8260869565217, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56118217, Length=345, Percent_Identity=27.8260869565217, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI189083684, Length=345, Percent_Identity=27.8260869565217, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI193211614, Length=298, Percent_Identity=24.8322147651007, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI8393516, Length=298, Percent_Identity=24.8322147651007, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI116268111, Length=305, Percent_Identity=26.8852459016393, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=27.5280898876405, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI48994969, Length=363, Percent_Identity=27.2727272727273, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786974, Length=324, Percent_Identity=25.6172839506173, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790049, Length=236, Percent_Identity=26.6949152542373, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71982035, Length=351, Percent_Identity=25.9259259259259, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71982038, Length=353, Percent_Identity=25.7790368271955, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=344, Percent_Identity=23.2558139534884, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=336, Percent_Identity=23.5119047619048, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=342, Percent_Identity=23.6842105263158, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=352, Percent_Identity=25.5681818181818, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=27.2988505747126, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=24.1071428571429, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37656; Mature: 37656

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGAAGFIGFHVSQALLARGDEVVGIDNLNDYYEVALKDARLAQLTPQEGFRFIRM
CEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCHHHHCCHHHCCEEEEE
DIGDRTAMAELFETEGFEKVVHLAAQAGVRYSLENPHAYADSNLTGFLNVLEGCRQTHVK
ECCCHHHHHHHHHHCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
HLVYASSSSVYGANESMPFAVQDNVDHPVSLYAATKKANELMAHSYSHLYRLPTTGLRFF
HHEEECCCCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHEECCCCCEEEE
TVYGPWGRPDMSPFLFVRAILEGKPLKVFNYGKHRRDFTYIDDIVDGVIRVLDHVAAPNA
EEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
QWSGLKPDPASSAAPWRLYNIGNSQPVELLDYIKHIEQALGKTTHKELLPLQPGDVEHTY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHH
ADVEQLKIDTGYAPDTPIESGIQRFVNWYKDFYNIS
HHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVTGAAGFIGFHVSQALLARGDEVVGIDNLNDYYEVALKDARLAQLTPQEGFRFIRM
CEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCHHHHCCHHHCCEEEEE
DIGDRTAMAELFETEGFEKVVHLAAQAGVRYSLENPHAYADSNLTGFLNVLEGCRQTHVK
ECCCHHHHHHHHHHCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
HLVYASSSSVYGANESMPFAVQDNVDHPVSLYAATKKANELMAHSYSHLYRLPTTGLRFF
HHEEECCCCCCCCCCCCCEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHEECCCCCEEEE
TVYGPWGRPDMSPFLFVRAILEGKPLKVFNYGKHRRDFTYIDDIVDGVIRVLDHVAAPNA
EEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
QWSGLKPDPASSAAPWRLYNIGNSQPVELLDYIKHIEQALGKTTHKELLPLQPGDVEHTY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHH
ADVEQLKIDTGYAPDTPIESGIQRFVNWYKDFYNIS
HHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]