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The map label for this gene is shiA [H]

Identifier: 77460118

GI number: 77460118

Start: 4407910

End: 4409220

Strand: Reverse

Name: shiA [H]

Synonym: Pfl01_3897

Alternate gene names: 77460118

Gene position: 4409220-4407910 (Counterclockwise)

Preceding gene: 77460120

Following gene: 77460117

Centisome position: 68.48

GC content: 64.07

Gene sequence:

>1311_bases
ATGAAGCCCCACGCTTCGAACCAGCCGCGCCGTGCGGCGGCCGCAGCCTTCATCGGCACCATGATCGAGTGGTACGACTT
TTACATCTACGCCACCGCCGCCGCGCTGGTGTTCGGCGCGCTGTTCTTTCCCTCCGACGATCCGCTGTTCAGCACCATGG
CCGCGTTCGGCACCTTCGCCGTGGGCTTCTTTGCCCGGCCATTGGGCGGGATCATTTTCGGTCATATCGGCGACCGCATC
GGCCGCAAGAAATCCCTGATCATCACGCTGTTGATGATGGGCGTGGTCACGGTGTGCATCGGTCTGCTGCCGACCTACGC
GCAGATCGGCGCGACGGCTCCGGTGCTGCTGATTCTGCTGCGTATCGTCCAGGGCATCGCCGTCGGTGGCGAGTGGGGCG
GGGCGGTGTTGATGGCTGGCGAACATGCGCCGAAGGGCCGGCGCAATTTCTTCGCTTCGTTCGCGCAACTGGGCAGTCCG
GCGGGTTTGATTCTGTCGCTGCTGGCCTTCAGCGCGGTGACCCGTTTGCCGGAAGAAGACCTGATGAGCTGGGGCTGGCG
CCTGCCGTTTCTCGCCAGTGCGCTGTTGCTGCTGGTGGGCCTGGCGATCCGACTGGGCGTGAACGAATCGCCGGAATTCC
TCGCCAGCCGCGAGCAGGCCAGCAAGCAAATCAAGAATGAACAAGCGCCGGTGATGGAAGTGTTGCGCACCGCGTGGCGT
CCGCTGTTGCTGTGCATCGGTGCCAACACGCTGGGCATCGCCGGGGTGTATTTCACCAACACCTTCATGATCGCCTACAC
CACTCAGCAACTGGCACTGCCGCGTTCGTTGATTCTCGAATGCCTGTTCTTCGTCGCGATCATCCAGTTCTGCGTCCAGC
CGCTGGCCGCGTGGACGGCGGAGAAGATCGGCGCGACGCGTTTCCTGTGCCTGGTGTCGCTGCTGGCGATGGCCTCGCCG
TACCCGATGTTCGTGCTGGTCAGTTCGGCCCAGGCGCCGCTGATCATCCTCGGCATCGCGCTGGCGGTGGTGTGCATGGC
CTCGTTCTACGCAGTGATCGCCGGCTACGTCAGCGGCATGTTCGAGACCCGCGTGCGCTACACCGCGATCTCGCTGGCCT
ATCAGATTTGCGGCGCGGTGGCCGGTGGGCTGACGCCGCTGATCGGCACGATGCTGGCGCACAAATTTACCGGGCAGTGG
TGGCCGATGGCGGTGTTCTACAGCCTGATCGCCGGCATTTCGCTGGTCTGCGTGCTGTCTCTCGCTCGTCGTCACGCCAA
CGTCCATCGCGCGGAAATGGCCCGTGCCTGA

Upstream 100 bases:

>100_bases
ATAGCCCCGGGACGATAAATCACCCCACGACAGGCGCCGCCAGAGCGCCTTGGATTTACGTTTTCCTCCTGCCAAAAAAA
CAATCAAAGGAGCCCGACCC

Downstream 100 bases:

>100_bases
CCCGGAATCTCAGGAGAACCTCATGTTGAAAATCAATGGCGAACGCCTGTGGGCGAGCCTGATGGCCATGGCCGAAATCG
GTGCGACAGCCCGAGGCGGC

Product: general substrate transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKPHASNQPRRAAAAAFIGTMIEWYDFYIYATAAALVFGALFFPSDDPLFSTMAAFGTFAVGFFARPLGGIIFGHIGDRI
GRKKSLIITLLMMGVVTVCIGLLPTYAQIGATAPVLLILLRIVQGIAVGGEWGGAVLMAGEHAPKGRRNFFASFAQLGSP
AGLILSLLAFSAVTRLPEEDLMSWGWRLPFLASALLLLVGLAIRLGVNESPEFLASREQASKQIKNEQAPVMEVLRTAWR
PLLLCIGANTLGIAGVYFTNTFMIAYTTQQLALPRSLILECLFFVAIIQFCVQPLAAWTAEKIGATRFLCLVSLLAMASP
YPMFVLVSSAQAPLIILGIALAVVCMASFYAVIAGYVSGMFETRVRYTAISLAYQICGAVAGGLTPLIGTMLAHKFTGQW
WPMAVFYSLIAGISLVCVLSLARRHANVHRAEMARA

Sequences:

>Translated_436_residues
MKPHASNQPRRAAAAAFIGTMIEWYDFYIYATAAALVFGALFFPSDDPLFSTMAAFGTFAVGFFARPLGGIIFGHIGDRI
GRKKSLIITLLMMGVVTVCIGLLPTYAQIGATAPVLLILLRIVQGIAVGGEWGGAVLMAGEHAPKGRRNFFASFAQLGSP
AGLILSLLAFSAVTRLPEEDLMSWGWRLPFLASALLLLVGLAIRLGVNESPEFLASREQASKQIKNEQAPVMEVLRTAWR
PLLLCIGANTLGIAGVYFTNTFMIAYTTQQLALPRSLILECLFFVAIIQFCVQPLAAWTAEKIGATRFLCLVSLLAMASP
YPMFVLVSSAQAPLIILGIALAVVCMASFYAVIAGYVSGMFETRVRYTAISLAYQICGAVAGGLTPLIGTMLAHKFTGQW
WPMAVFYSLIAGISLVCVLSLARRHANVHRAEMARA
>Mature_436_residues
MKPHASNQPRRAAAAAFIGTMIEWYDFYIYATAAALVFGALFFPSDDPLFSTMAAFGTFAVGFFARPLGGIIFGHIGDRI
GRKKSLIITLLMMGVVTVCIGLLPTYAQIGATAPVLLILLRIVQGIAVGGEWGGAVLMAGEHAPKGRRNFFASFAQLGSP
AGLILSLLAFSAVTRLPEEDLMSWGWRLPFLASALLLLVGLAIRLGVNESPEFLASREQASKQIKNEQAPVMEVLRTAWR
PLLLCIGANTLGIAGVYFTNTFMIAYTTQQLALPRSLILECLFFVAIIQFCVQPLAAWTAEKIGATRFLCLVSLLAMASP
YPMFVLVSSAQAPLIILGIALAVVCMASFYAVIAGYVSGMFETRVRYTAISLAYQICGAVAGGLTPLIGTMLAHKFTGQW
WPMAVFYSLIAGISLVCVLSLARRHANVHRAEMARA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=38.7019230769231, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1790550, Length=410, Percent_Identity=33.6585365853659, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=36.046511627907, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=31.322505800464, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87082231, Length=237, Percent_Identity=24.0506329113924, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46943; Mature: 46943

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPHASNQPRRAAAAAFIGTMIEWYDFYIYATAAALVFGALFFPSDDPLFSTMAAFGTFA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VGFFARPLGGIIFGHIGDRIGRKKSLIITLLMMGVVTVCIGLLPTYAQIGATAPVLLILL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RIVQGIAVGGEWGGAVLMAGEHAPKGRRNFFASFAQLGSPAGLILSLLAFSAVTRLPEED
HHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
LMSWGWRLPFLASALLLLVGLAIRLGVNESPEFLASREQASKQIKNEQAPVMEVLRTAWR
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
PLLLCIGANTLGIAGVYFTNTFMIAYTTQQLALPRSLILECLFFVAIIQFCVQPLAAWTA
HHHHHHCCCHHHHHHHHHHHEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EKIGATRFLCLVSLLAMASPYPMFVLVSSAQAPLIILGIALAVVCMASFYAVIAGYVSGM
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FETRVRYTAISLAYQICGAVAGGLTPLIGTMLAHKFTGQWWPMAVFYSLIAGISLVCVLS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LARRHANVHRAEMARA
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPHASNQPRRAAAAAFIGTMIEWYDFYIYATAAALVFGALFFPSDDPLFSTMAAFGTFA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VGFFARPLGGIIFGHIGDRIGRKKSLIITLLMMGVVTVCIGLLPTYAQIGATAPVLLILL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RIVQGIAVGGEWGGAVLMAGEHAPKGRRNFFASFAQLGSPAGLILSLLAFSAVTRLPEED
HHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHH
LMSWGWRLPFLASALLLLVGLAIRLGVNESPEFLASREQASKQIKNEQAPVMEVLRTAWR
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
PLLLCIGANTLGIAGVYFTNTFMIAYTTQQLALPRSLILECLFFVAIIQFCVQPLAAWTA
HHHHHHCCCHHHHHHHHHHHEEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EKIGATRFLCLVSLLAMASPYPMFVLVSSAQAPLIILGIALAVVCMASFYAVIAGYVSGM
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FETRVRYTAISLAYQICGAVAGGLTPLIGTMLAHKFTGQWWPMAVFYSLIAGISLVCVLS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LARRHANVHRAEMARA
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]