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The map label for this gene is czcS [H]

Identifier: 77459602

GI number: 77459602

Start: 3853752

End: 3855167

Strand: Direct

Name: czcS [H]

Synonym: Pfl01_3380

Alternate gene names: 77459602

Gene position: 3853752-3855167 (Clockwise)

Preceding gene: 77459601

Following gene: 77459604

Centisome position: 59.86

GC content: 64.12

Gene sequence:

>1416_bases
ATGAAAATCGCCGGCACGTATTCCCTGACCTTGCGTCTGGCGCTGGTCTTCGCCGTGCTCGCCTTTGCCGCGCTGGGAGG
CCTGGGCGCGGCGCTGTACAACCAACTGGAAGCAGCTCTGATTCGCCGCGATGACATCCAGTTGGTCAGTCGCGTCGATC
AGTTGCGCACCTTCCTCAACGACAGCAACACCCTGGAACTGATCAAGAACAAGCCGGCGCTGTTTCAGAACATGCTCGGC
AACCGCGAAGCCTTGCTGACCATCGGCGCACCCGGCCAGCCGCCGTTGCTGGTGGTCAACCCCGGCAACCTCGCCACCCC
GAACTTGCCCGCCGTACCGATCGATCATCCGATGGCCCTGAGCGATGTACAGCACTTGCCGAGCGTCGACGGTGTGCCGT
TTTCGGCGGTGGCGGCGACCATCGACTCGGGCGATCTGGGCAACCTGCAGGTGCTCACCGGACGCCTGATGACCGAGCGC
ACGGCGATGCTCGCCAATTACCGGCTCAGCGTGTACGGCCTCGCGTCATTGGCCGCGATTGTGCTGGCGCTGGTCGGCTG
TCTGCTGGTGTATCGCGGCCTGCTGCCGTTGCGGCGGATGGCCCGGCATGCCCACGGGATCGGCATCGCCAACCTTGGCG
AACGCCTCGACCGCCAAGGCACGCCTCGTGAATTGCAGCCGATGATCGAAGCCTTCAACGCGATGCTGGACCGGCTCGAT
CGAGGGGTTGCGCAACTGAGCCAGGTCTCCACCGACATGGCCCACGAACTGCGCACGCCGATCAACAACCTGCTCGGGGA
AACACAGGTCGCGTTGCAACAGAACCGCAGCGTCGACGCCTATCAGCAGTTGCTCGCCTCCAACGTGGAAGAACTCGAAC
GGCTGGCGCGGATGCTCGACAACATGCTGTTTCTCGCCCGCACCGACCCGGCCAGCGCGCTGAGCCAGCGGCAGGAGCTG
GACGCCAGCGATGAGATGCAACGCATTGCCGATTACTTCGAAGGATTGGCGGCGGATGTCGGCGTCACCATCGAGGCCAG
TGGCAGCGGCGTGATCTGGGCCGAGCCGATGCTGCTGCGGCGGGCCCTGGCCAACCTGTGCGCCAACGCGATCAAGTACG
GCGCGGCGGATTCGACGTTGCAGGTCGAAGCCATCGCTGAAACGGACGGCAGCTATCTGCGGGTGCGCAACCAGGGCACG
ACCATTCCTGCCGAACACCTGTCGCGACTGTTCGAACGCTTCTACCGCGTCGATCCGTCACGCGAGCGCTCGGCCCAGTC
CAACGGCTTGGGCCTGTCGATTGTCGCGACCATCATGCAGTTGCATCAGGGCCGTTACAGCGTCAGCAGCGCTGATGGCA
TCACCTGCTTTGAACTGTTCTTTCCGGCACGGGAGACGCGAGACGCAGGTGCGTGA

Upstream 100 bases:

>100_bases
ATGTCGCCGTGCGCCGCCTGCGGGCGAAGATCGACGATCCGTTCCCGCTCAAGCTGATCCACAGCGTGCGCGGCATCGGC
TACCGCTTCGATACCCAACC

Downstream 100 bases:

>100_bases
AGGCGCCGAGCGCAGACGCCGCGAACATCAACGCCGTAGCCACACCGAAGGTCATGTGCAAGCCGCCTGCGATGACCGCG
GCCGGCGCCTCAGTCGGATC

Product: heavy metal sensor Signal transduction histidine kinase (STHK)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKIAGTYSLTLRLALVFAVLAFAALGGLGAALYNQLEAALIRRDDIQLVSRVDQLRTFLNDSNTLELIKNKPALFQNMLG
NREALLTIGAPGQPPLLVVNPGNLATPNLPAVPIDHPMALSDVQHLPSVDGVPFSAVAATIDSGDLGNLQVLTGRLMTER
TAMLANYRLSVYGLASLAAIVLALVGCLLVYRGLLPLRRMARHAHGIGIANLGERLDRQGTPRELQPMIEAFNAMLDRLD
RGVAQLSQVSTDMAHELRTPINNLLGETQVALQQNRSVDAYQQLLASNVEELERLARMLDNMLFLARTDPASALSQRQEL
DASDEMQRIADYFEGLAADVGVTIEASGSGVIWAEPMLLRRALANLCANAIKYGAADSTLQVEAIAETDGSYLRVRNQGT
TIPAEHLSRLFERFYRVDPSRERSAQSNGLGLSIVATIMQLHQGRYSVSSADGITCFELFFPARETRDAGA

Sequences:

>Translated_471_residues
MKIAGTYSLTLRLALVFAVLAFAALGGLGAALYNQLEAALIRRDDIQLVSRVDQLRTFLNDSNTLELIKNKPALFQNMLG
NREALLTIGAPGQPPLLVVNPGNLATPNLPAVPIDHPMALSDVQHLPSVDGVPFSAVAATIDSGDLGNLQVLTGRLMTER
TAMLANYRLSVYGLASLAAIVLALVGCLLVYRGLLPLRRMARHAHGIGIANLGERLDRQGTPRELQPMIEAFNAMLDRLD
RGVAQLSQVSTDMAHELRTPINNLLGETQVALQQNRSVDAYQQLLASNVEELERLARMLDNMLFLARTDPASALSQRQEL
DASDEMQRIADYFEGLAADVGVTIEASGSGVIWAEPMLLRRALANLCANAIKYGAADSTLQVEAIAETDGSYLRVRNQGT
TIPAEHLSRLFERFYRVDPSRERSAQSNGLGLSIVATIMQLHQGRYSVSSADGITCFELFFPARETRDAGA
>Mature_471_residues
MKIAGTYSLTLRLALVFAVLAFAALGGLGAALYNQLEAALIRRDDIQLVSRVDQLRTFLNDSNTLELIKNKPALFQNMLG
NREALLTIGAPGQPPLLVVNPGNLATPNLPAVPIDHPMALSDVQHLPSVDGVPFSAVAATIDSGDLGNLQVLTGRLMTER
TAMLANYRLSVYGLASLAAIVLALVGCLLVYRGLLPLRRMARHAHGIGIANLGERLDRQGTPRELQPMIEAFNAMLDRLD
RGVAQLSQVSTDMAHELRTPINNLLGETQVALQQNRSVDAYQQLLASNVEELERLARMLDNMLFLARTDPASALSQRQEL
DASDEMQRIADYFEGLAADVGVTIEASGSGVIWAEPMLLRRALANLCANAIKYGAADSTLQVEAIAETDGSYLRVRNQGT
TIPAEHLSRLFERFYRVDPSRERSAQSNGLGLSIVATIMQLHQGRYSVSSADGITCFELFFPARETRDAGA

Specific function: Member of the two-component regulatory system CzcS/CzcR involved in the control of cobalt, zinc and cadmium homeostasis. Probably activates CzcR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=290, Percent_Identity=39.6551724137931, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1788279, Length=457, Percent_Identity=31.0722100656455, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1790346, Length=253, Percent_Identity=28.0632411067194, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1789403, Length=448, Percent_Identity=26.5625, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788393, Length=260, Percent_Identity=26.5384615384615, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786600, Length=239, Percent_Identity=26.3598326359833, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082128, Length=298, Percent_Identity=27.1812080536913, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787894, Length=277, Percent_Identity=29.2418772563177, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790861, Length=231, Percent_Identity=26.4069264069264, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI145693157, Length=238, Percent_Identity=26.890756302521, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789808, Length=306, Percent_Identity=26.797385620915, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=213, Percent_Identity=24.4131455399061, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788713, Length=254, Percent_Identity=22.0472440944882, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790551, Length=237, Percent_Identity=27.8481012658228, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789149, Length=246, Percent_Identity=24.390243902439, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51124; Mature: 51124

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAGTYSLTLRLALVFAVLAFAALGGLGAALYNQLEAALIRRDDIQLVSRVDQLRTFLN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DSNTLELIKNKPALFQNMLGNREALLTIGAPGQPPLLVVNPGNLATPNLPAVPIDHPMAL
CCCHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCHH
SDVQHLPSVDGVPFSAVAATIDSGDLGNLQVLTGRLMTERTAMLANYRLSVYGLASLAAI
HHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLALVGCLLVYRGLLPLRRMARHAHGIGIANLGERLDRQGTPRELQPMIEAFNAMLDRLD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RGVAQLSQVSTDMAHELRTPINNLLGETQVALQQNRSVDAYQQLLASNVEELERLARMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHH
NMLFLARTDPASALSQRQELDASDEMQRIADYFEGLAADVGVTIEASGSGVIWAEPMLLR
HHEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCHHHHH
RALANLCANAIKYGAADSTLQVEAIAETDGSYLRVRNQGTTIPAEHLSRLFERFYRVDPS
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCC
RERSAQSNGLGLSIVATIMQLHQGRYSVSSADGITCFELFFPARETRDAGA
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHCCCCCC
>Mature Secondary Structure
MKIAGTYSLTLRLALVFAVLAFAALGGLGAALYNQLEAALIRRDDIQLVSRVDQLRTFLN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DSNTLELIKNKPALFQNMLGNREALLTIGAPGQPPLLVVNPGNLATPNLPAVPIDHPMAL
CCCHHHHHHCCHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCHH
SDVQHLPSVDGVPFSAVAATIDSGDLGNLQVLTGRLMTERTAMLANYRLSVYGLASLAAI
HHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLALVGCLLVYRGLLPLRRMARHAHGIGIANLGERLDRQGTPRELQPMIEAFNAMLDRLD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RGVAQLSQVSTDMAHELRTPINNLLGETQVALQQNRSVDAYQQLLASNVEELERLARMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHH
NMLFLARTDPASALSQRQELDASDEMQRIADYFEGLAADVGVTIEASGSGVIWAEPMLLR
HHEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEECCHHHHH
RALANLCANAIKYGAADSTLQVEAIAETDGSYLRVRNQGTTIPAEHLSRLFERFYRVDPS
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCC
RERSAQSNGLGLSIVATIMQLHQGRYSVSSADGITCFELFFPARETRDAGA
HHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9044283 [H]