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The map label for this gene is gcvA [H]

Identifier: 77459504

GI number: 77459504

Start: 3767543

End: 3768427

Strand: Direct

Name: gcvA [H]

Synonym: Pfl01_3282

Alternate gene names: 77459504

Gene position: 3767543-3768427 (Clockwise)

Preceding gene: 77459501

Following gene: 77459507

Centisome position: 58.52

GC content: 62.82

Gene sequence:

>885_bases
ATGAATCCGCGAACCCTCACTCCCTCGATGTCGTTGCTGCTGGCGTTTGAAGCCGCCGCGCGCCATGAAAGCTACACCCG
CGCCGCCCACGAACTGTCGCTGACCCAGAGCGCCGTCAGCCGGCAAGTGCAGATTCTGGAAAAGATGCTCGGCATGCGCT
TGTTCAGCCGCGAAGGCCGGCGGGTGATCCTTACCGACGTCGGGCGCATGTATCAGCGCGAACTGTCCGAAGCCCTCGGC
CAGATCCGCAGTGCGACCTTGCAGGCCATGGCGTTTGGCTCAGGCATCCACAGTTTGCGCCTGGCGACCCTGCCGACCTT
CGGCTCGAAATGGTTGCTGCCGCGCTTGAAGGATTTCTACACCGCGCACCCGGGCATGACCGTTCACCTGCATTCACGCA
TCGAAACCATCGACTTCGACACCAGCGAAATCGACGCGGCGATTTGCGTCGGTGGCGGCGACTGGCCGGGGCTGACCGCT
CACCGCCTGCACACCGAAGAACTGGTGGTGATCGCCAGCGCGCAGCTGTCCGATGCCGAACGCCGTGCGGCCGACAAGGA
CATCGCCGGGCAACTGCTGCTCAACGTCAGCAGCAACGCTCAGGCCTGGTCCGAGTGGTTCAGCCATCATGCCCTGCCCC
ATCGCAGCATGCGCATTGGCCCGAGTTTCGAGATGACCTCGCACTTGATCCAGGCCGTGCGCGCCAATATCGGGATCGGG
CTGGTGCCGAGGATTCTGGTGGAAGATGAATTGCACAACGGCGAGCTATTGCAACTGGGTGAACCGATCAGCAGCCGGCG
CAGCTATTACCTGGTGTACCCGGCGCGCAATGAGTCGCTGGCGTCGCTGAAGGCGTTTCGCGACTGGTTGATGCGCACGC
TTTGA

Upstream 100 bases:

>100_bases
ATGATTGCGTCACGCTCCCGCCATCTCATAGGCCTTATGTTCATTGCAGAGCGATGTCGGGACAACCGTAAAATCGACCC
GCAATCCATGACTAAAACGA

Downstream 100 bases:

>100_bases
AACAACCGAGGGCGCCGTTTGGCGCCCTCTGGTTTTGCTCGATCAGAATCGTGGCTTCAGCGCTTTCCAGTCAGGTTTAT
AGCGTTGCATCTGCCGCACA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNPRTLTPSMSLLLAFEAAARHESYTRAAHELSLTQSAVSRQVQILEKMLGMRLFSREGRRVILTDVGRMYQRELSEALG
QIRSATLQAMAFGSGIHSLRLATLPTFGSKWLLPRLKDFYTAHPGMTVHLHSRIETIDFDTSEIDAAICVGGGDWPGLTA
HRLHTEELVVIASAQLSDAERRAADKDIAGQLLLNVSSNAQAWSEWFSHHALPHRSMRIGPSFEMTSHLIQAVRANIGIG
LVPRILVEDELHNGELLQLGEPISSRRSYYLVYPARNESLASLKAFRDWLMRTL

Sequences:

>Translated_294_residues
MNPRTLTPSMSLLLAFEAAARHESYTRAAHELSLTQSAVSRQVQILEKMLGMRLFSREGRRVILTDVGRMYQRELSEALG
QIRSATLQAMAFGSGIHSLRLATLPTFGSKWLLPRLKDFYTAHPGMTVHLHSRIETIDFDTSEIDAAICVGGGDWPGLTA
HRLHTEELVVIASAQLSDAERRAADKDIAGQLLLNVSSNAQAWSEWFSHHALPHRSMRIGPSFEMTSHLIQAVRANIGIG
LVPRILVEDELHNGELLQLGEPISSRRSYYLVYPARNESLASLKAFRDWLMRTL
>Mature_294_residues
MNPRTLTPSMSLLLAFEAAARHESYTRAAHELSLTQSAVSRQVQILEKMLGMRLFSREGRRVILTDVGRMYQRELSEALG
QIRSATLQAMAFGSGIHSLRLATLPTFGSKWLLPRLKDFYTAHPGMTVHLHSRIETIDFDTSEIDAAICVGGGDWPGLTA
HRLHTEELVVIASAQLSDAERRAADKDIAGQLLLNVSSNAQAWSEWFSHHALPHRSMRIGPSFEMTSHLIQAVRANIGIG
LVPRILVEDELHNGELLQLGEPISSRRSYYLVYPARNESLASLKAFRDWLMRTL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=33.5664335664336, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=29.2096219931272, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=29.2517006802721, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=35.7575757575758, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=26.962457337884, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87082132, Length=244, Percent_Identity=27.0491803278689, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789639, Length=274, Percent_Identity=25.9124087591241, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=26.027397260274, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=25.7042253521127, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32875; Mature: 32875

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRTLTPSMSLLLAFEAAARHESYTRAAHELSLTQSAVSRQVQILEKMLGMRLFSREGR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RVILTDVGRMYQRELSEALGQIRSATLQAMAFGSGIHSLRLATLPTFGSKWLLPRLKDFY
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCHHHHHHHH
TAHPGMTVHLHSRIETIDFDTSEIDAAICVGGGDWPGLTAHRLHTEELVVIASAQLSDAE
HCCCCEEEEEECCEEEECCCCHHCCEEEEECCCCCCCCCHHHCCCCCEEEEEECHHCHHH
RRAADKDIAGQLLLNVSSNAQAWSEWFSHHALPHRSMRIGPSFEMTSHLIQAVRANIGIG
HHHCCHHHHHHEEEECCCCHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHCCCCC
LVPRILVEDELHNGELLQLGEPISSRRSYYLVYPARNESLASLKAFRDWLMRTL
HHHHHHHHCCCCCCCEEECCCCHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPRTLTPSMSLLLAFEAAARHESYTRAAHELSLTQSAVSRQVQILEKMLGMRLFSREGR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RVILTDVGRMYQRELSEALGQIRSATLQAMAFGSGIHSLRLATLPTFGSKWLLPRLKDFY
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCCCCCCHHHHHHHH
TAHPGMTVHLHSRIETIDFDTSEIDAAICVGGGDWPGLTAHRLHTEELVVIASAQLSDAE
HCCCCEEEEEECCEEEECCCCHHCCEEEEECCCCCCCCCHHHCCCCCEEEEEECHHCHHH
RRAADKDIAGQLLLNVSSNAQAWSEWFSHHALPHRSMRIGPSFEMTSHLIQAVRANIGIG
HHHCCHHHHHHEEEECCCCHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHCCCCC
LVPRILVEDELHNGELLQLGEPISSRRSYYLVYPARNESLASLKAFRDWLMRTL
HHHHHHHHCCCCCCCEEECCCCHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]