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The map label for this gene is afr [H]

Identifier: 77459387

GI number: 77459387

Start: 3633663

End: 3634631

Strand: Reverse

Name: afr [H]

Synonym: Pfl01_3165

Alternate gene names: 77459387

Gene position: 3634631-3633663 (Counterclockwise)

Preceding gene: 77459388

Following gene: 77459386

Centisome position: 56.45

GC content: 64.19

Gene sequence:

>969_bases
ATGAACGTGGTGCGTTGGGACATGATCGGTTGCGGCAGCGTCGCCGAACGCAAGAGCGGGCCGGCCTTCTACAAGGCGCC
CGGCTCGGCGTTGGTGGCGGTGATGGGGCGACGTCTCGAAGCGGTGACCGATTACGCCGCGCGCCACGGCATCGCTCGGA
CGTACACCGACGTCGATGCCCTGATCAACGACCCCGAGGTGGACGCGGTGTACATCGCCACACCACCCGATAGCCACCAC
ACCTACAGCCTGAAAGTCGCCGCCGCCGGCAAGCACTGCTGCGTGGAGAAACCCATGGCGCTCAACGCCGGGCAAAGCCG
CGAGATGCAGCAGGCGTTCGCCGAGGCTGGTCTGCACCTGTTCGTCTCCTATTACCGCCGTTCGTTACCGCGTTTCCAGC
AGGTTCGGCAGTGGCTGGAGCAGGGCCGCATCGGCCAGGTGCGGCATCTGAGCTGGACGCTGACCAAGGCACCATCGCCC
AAGGATCTGGACGGCAGCGCAAACTGGCGCACCGATCCGGCGGTCGCCGGGGGCGGGTATTTCGCGGATCTGGCCAGCCA
TGGTTTTGACCTGTTCCAGTACCTGCTTGGCGACATCGTCGAAGTCGCCGGTTTCACCGCGCGTCAGGCCGGGTTGTACG
CCGCGGAGGATGCGGTCAGCGCCAGTTGGCGATTCGCCTCGGGCGCGCTGGGCATGGGTTGCTGGAGCTTTGTTGCGGAT
CGCCGTGAGGATCGGGTCGAGATCATTGGCAGCCAAGGGCGGATCGGCTTTTCGGTGTTCGACGAGCATCCGGTCGAGCT
GCATGCCGACGAACACATCAGCCTGGAAATCCCCCATCACGAACACATCCAGTGGCATCACGTACTCGGTATGAACGCGC
AGATTCGCGGTGAGTCGCAGCACCCGGCAATCGCCGCCGAAGCCCTGAAGACCGATTGGGTCATGGATCAGATCCTCAAA
CGCCACTGA

Upstream 100 bases:

>100_bases
TCGCATCAGCCGGCCGATCACCGGCGGCTATTACTGGTGCCCGCCGCTGCAAAACGGCCACCTCGATCTGCGCGCACTGC
GCATCGGCTGAGGCACAAGC

Downstream 100 bases:

>100_bases
AGGCGCATCTAACCTTATGCTCATTCGGTATTTCTCAAGCTTGTCATAACGTCTCTAAGATCACGTCATAACTCGTTATG
ACAAGTGATTCCGTGATGAC

Product: oxidoreductase-like protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNVVRWDMIGCGSVAERKSGPAFYKAPGSALVAVMGRRLEAVTDYAARHGIARTYTDVDALINDPEVDAVYIATPPDSHH
TYSLKVAAAGKHCCVEKPMALNAGQSREMQQAFAEAGLHLFVSYYRRSLPRFQQVRQWLEQGRIGQVRHLSWTLTKAPSP
KDLDGSANWRTDPAVAGGGYFADLASHGFDLFQYLLGDIVEVAGFTARQAGLYAAEDAVSASWRFASGALGMGCWSFVAD
RREDRVEIIGSQGRIGFSVFDEHPVELHADEHISLEIPHHEHIQWHHVLGMNAQIRGESQHPAIAAEALKTDWVMDQILK
RH

Sequences:

>Translated_322_residues
MNVVRWDMIGCGSVAERKSGPAFYKAPGSALVAVMGRRLEAVTDYAARHGIARTYTDVDALINDPEVDAVYIATPPDSHH
TYSLKVAAAGKHCCVEKPMALNAGQSREMQQAFAEAGLHLFVSYYRRSLPRFQQVRQWLEQGRIGQVRHLSWTLTKAPSP
KDLDGSANWRTDPAVAGGGYFADLASHGFDLFQYLLGDIVEVAGFTARQAGLYAAEDAVSASWRFASGALGMGCWSFVAD
RREDRVEIIGSQGRIGFSVFDEHPVELHADEHISLEIPHHEHIQWHHVLGMNAQIRGESQHPAIAAEALKTDWVMDQILK
RH
>Mature_322_residues
MNVVRWDMIGCGSVAERKSGPAFYKAPGSALVAVMGRRLEAVTDYAARHGIARTYTDVDALINDPEVDAVYIATPPDSHH
TYSLKVAAAGKHCCVEKPMALNAGQSREMQQAFAEAGLHLFVSYYRRSLPRFQQVRQWLEQGRIGQVRHLSWTLTKAPSP
KDLDGSANWRTDPAVAGGGYFADLASHGFDLFQYLLGDIVEVAGFTARQAGLYAAEDAVSASWRFASGALGMGCWSFVAD
RREDRVEIIGSQGRIGFSVFDEHPVELHADEHISLEIPHHEHIQWHHVLGMNAQIRGESQHPAIAAEALKTDWVMDQILK
RH

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=147, Percent_Identity=29.9319727891156, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787574, Length=337, Percent_Identity=24.6290801186944, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082405, Length=228, Percent_Identity=27.1929824561404, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081947, Length=195, Percent_Identity=28.7179487179487, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789848, Length=199, Percent_Identity=26.1306532663317, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323975, Length=269, Percent_Identity=23.4200743494424, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581117, Length=232, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 35628; Mature: 35628

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVRWDMIGCGSVAERKSGPAFYKAPGSALVAVMGRRLEAVTDYAARHGIARTYTDVDA
CCCEEEECCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LINDPEVDAVYIATPPDSHHTYSLKVAAAGKHCCVEKPMALNAGQSREMQQAFAEAGLHL
HCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FVSYYRRSLPRFQQVRQWLEQGRIGQVRHLSWTLTKAPSPKDLDGSANWRTDPAVAGGGY
HHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
FADLASHGFDLFQYLLGDIVEVAGFTARQAGLYAAEDAVSASWRFASGALGMGCWSFVAD
HHHHHHCHHHHHHHHHHHHHHHHCCHHHHCCCEEHHHHHCCCEEECCCCCHHHHHHHHHH
RREDRVEIIGSQGRIGFSVFDEHPVELHADEHISLEIPHHEHIQWHHVLGMNAQIRGESQ
CCCCCEEEECCCCCEEEEECCCCCEEEECCCEEEEECCCCCCCEEEEEECCCCEECCCCC
HPAIAAEALKTDWVMDQILKRH
CCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNVVRWDMIGCGSVAERKSGPAFYKAPGSALVAVMGRRLEAVTDYAARHGIARTYTDVDA
CCCEEEECCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LINDPEVDAVYIATPPDSHHTYSLKVAAAGKHCCVEKPMALNAGQSREMQQAFAEAGLHL
HCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FVSYYRRSLPRFQQVRQWLEQGRIGQVRHLSWTLTKAPSPKDLDGSANWRTDPAVAGGGY
HHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
FADLASHGFDLFQYLLGDIVEVAGFTARQAGLYAAEDAVSASWRFASGALGMGCWSFVAD
HHHHHHCHHHHHHHHHHHHHHHHCCHHHHCCCEEHHHHHCCCEEECCCCCHHHHHHHHHH
RREDRVEIIGSQGRIGFSVFDEHPVELHADEHISLEIPHHEHIQWHHVLGMNAQIRGESQ
CCCCCEEEECCCCCEEEEECCCCCEEEECCCEEEEECCCCCCCEEEEEECCCCEECCCCC
HPAIAAEALKTDWVMDQILKRH
CCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]