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The map label for this gene is proP [H]

Identifier: 77459209

GI number: 77459209

Start: 3428779

End: 3430128

Strand: Direct

Name: proP [H]

Synonym: Pfl01_2986

Alternate gene names: 77459209

Gene position: 3428779-3430128 (Clockwise)

Preceding gene: 77459206

Following gene: 77459210

Centisome position: 53.26

GC content: 54.37

Gene sequence:

>1350_bases
ATGAACAACAATAACGTTATGGCCGAGCCCGTTTCACTAGCCGTTGAAGAAACGGAACAAACGAGAAAAGCCAAAAAAGC
CACAACGGCGGCTGTATTTGGGACTTTCGTTGAGTATTACGACTTCAGCGTGTACGGGTATGTCGCAGCAACGCTCGCGA
TGGTTTTCTTCCCTTCGGACAATGCAACGATTGGCCTGCTGAATACCTTCCTAGTATTCGGGTCTGCCTTTCTGGTGAGG
CCGCTCGGCGCAATTGCTTTCGGCTGGCTTGGTGACAAGGTAGGAAGGCGCTTCAGCCTGATTGCCAGCATTACCTTAAT
GGGAGCTGCAGCCACGCTCATTGGCCTGCTGCCCGGCTATGCGCAGATTGGCATTTGGGCGCCGATACTGTTAGTCGTCC
TACGCATGCTCCAAGGGTTCTCCGCTGGTGGCGAAATCGGCGGCGCTGCGAGCTACATCCGTGAGTGGGCACCGCCTAAA
CGCCGCGCGTTGTACATATCCTTCCTGCCCTCCATCGCTCAGTTCGGTAAAGGTTTGGCGGCGGCAATTGCCGGTCTTAC
AGCGGCAACCCTCACGGATCCAGAAATGCTCTCCTGGGGCTGGCGTATTCCGTTCCTGTTAGCTCTGCCGCTGGGAATCA
TCGGTCTTTACATGCGACTGAGCATTGAGGACAGCCCTGAGTTCGCATCGAAGAAAAACGATGCAGAAGTCAAGAAAGGT
AAGCCCTTTGCTGAGTTGATTCGGGACTATCGCAAGCCTTTAGCAAAAGTGATCATGATCTCAATGACGCAGAACATCGG
CACATACATCGGCACCGTATTCATCGCGGCGTACTTCAGCTCAATCTTGGGATACTCGAAAGGTGAAGCGTCCACCATTG
TATTGATTGCAGTGATTTTCGCTGCATTTTTGATCCCTCTTGCTGGCCAGTTGGGTTCCTATCGCGGCGGCAGGTCTGTC
CTGCGGTATGCCTACGTGGCTTATGCCGTGTTGTCGATACCCTCGTTCATGCTCATGCAGCAAGGCTCGGTCAATCTGGC
AATTGCTGGGCTGGCGCTCGGCATGATCCCGTATGCGCTCTGCCAAGCGGGCACCTATTCGGTAATGCCGGAGTTTTTCC
CGATGCATATTCGCCACACGGGCGTCGCATTCGGCCACAGCGTAGGCGCCGTTATCGGCGGAGGTTCTGGCCCATTCCTA
GCGACCTGGCTTATCGGAGCCACCGGTAATCAGTCCACCCCCGCATTCATTTTGTGCGCGTCAGGTCTATTGGGGCTGAT
CGTTATCTCGACCGTTCGTAAAAATGCGCCGGTAGCTAGCGATGACGTCAATACCCACCAATTCTCATAA

Upstream 100 bases:

>100_bases
CTGTTGACAACTATCCCGGCGTTCTAGAAGCTAGACAAAACAAGAGCAGGACACTGTCCCATTTTTTGAATGAGACAGAC
ATCCTATAACCGAGGTGCCC

Downstream 100 bases:

>100_bases
GGTGACGATCATGTCTTTGATTTATGTGACTAGCCCGATCCATCCAGAAGTACTACAGGCCCTTTCGTCGGTGGGCGAAG
TTCGACTCGGCTATGGCCCA

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNNNNVMAEPVSLAVEETEQTRKAKKATTAAVFGTFVEYYDFSVYGYVAATLAMVFFPSDNATIGLLNTFLVFGSAFLVR
PLGAIAFGWLGDKVGRRFSLIASITLMGAAATLIGLLPGYAQIGIWAPILLVVLRMLQGFSAGGEIGGAASYIREWAPPK
RRALYISFLPSIAQFGKGLAAAIAGLTAATLTDPEMLSWGWRIPFLLALPLGIIGLYMRLSIEDSPEFASKKNDAEVKKG
KPFAELIRDYRKPLAKVIMISMTQNIGTYIGTVFIAAYFSSILGYSKGEASTIVLIAVIFAAFLIPLAGQLGSYRGGRSV
LRYAYVAYAVLSIPSFMLMQQGSVNLAIAGLALGMIPYALCQAGTYSVMPEFFPMHIRHTGVAFGHSVGAVIGGGSGPFL
ATWLIGATGNQSTPAFILCASGLLGLIVISTVRKNAPVASDDVNTHQFS

Sequences:

>Translated_449_residues
MNNNNVMAEPVSLAVEETEQTRKAKKATTAAVFGTFVEYYDFSVYGYVAATLAMVFFPSDNATIGLLNTFLVFGSAFLVR
PLGAIAFGWLGDKVGRRFSLIASITLMGAAATLIGLLPGYAQIGIWAPILLVVLRMLQGFSAGGEIGGAASYIREWAPPK
RRALYISFLPSIAQFGKGLAAAIAGLTAATLTDPEMLSWGWRIPFLLALPLGIIGLYMRLSIEDSPEFASKKNDAEVKKG
KPFAELIRDYRKPLAKVIMISMTQNIGTYIGTVFIAAYFSSILGYSKGEASTIVLIAVIFAAFLIPLAGQLGSYRGGRSV
LRYAYVAYAVLSIPSFMLMQQGSVNLAIAGLALGMIPYALCQAGTYSVMPEFFPMHIRHTGVAFGHSVGAVIGGGSGPFL
ATWLIGATGNQSTPAFILCASGLLGLIVISTVRKNAPVASDDVNTHQFS
>Mature_449_residues
MNNNNVMAEPVSLAVEETEQTRKAKKATTAAVFGTFVEYYDFSVYGYVAATLAMVFFPSDNATIGLLNTFLVFGSAFLVR
PLGAIAFGWLGDKVGRRFSLIASITLMGAAATLIGLLPGYAQIGIWAPILLVVLRMLQGFSAGGEIGGAASYIREWAPPK
RRALYISFLPSIAQFGKGLAAAIAGLTAATLTDPEMLSWGWRIPFLLALPLGIIGLYMRLSIEDSPEFASKKNDAEVKKG
KPFAELIRDYRKPLAKVIMISMTQNIGTYIGTVFIAAYFSSILGYSKGEASTIVLIAVIFAAFLIPLAGQLGSYRGGRSV
LRYAYVAYAVLSIPSFMLMQQGSVNLAIAGLALGMIPYALCQAGTYSVMPEFFPMHIRHTGVAFGHSVGAVIGGGSGPFL
ATWLIGATGNQSTPAFILCASGLLGLIVISTVRKNAPVASDDVNTHQFS

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=449, Percent_Identity=34.9665924276169, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1788292, Length=413, Percent_Identity=33.6561743341404, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789941, Length=395, Percent_Identity=34.6835443037975, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788942, Length=403, Percent_Identity=32.258064516129, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI87082231, Length=437, Percent_Identity=22.4256292906178, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47769; Mature: 47769

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNNVMAEPVSLAVEETEQTRKAKKATTAAVFGTFVEYYDFSVYGYVAATLAMVFFPSD
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
NATIGLLNTFLVFGSAFLVRPLGAIAFGWLGDKVGRRFSLIASITLMGAAATLIGLLPGY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AQIGIWAPILLVVLRMLQGFSAGGEIGGAASYIREWAPPKRRALYISFLPSIAQFGKGLA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHEEHHHHHHHHHHHCCHHH
AAIAGLTAATLTDPEMLSWGWRIPFLLALPLGIIGLYMRLSIEDSPEFASKKNDAEVKKG
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHCCCCCCCCCCC
KPFAELIRDYRKPLAKVIMISMTQNIGTYIGTVFIAAYFSSILGYSKGEASTIVLIAVIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AAFLIPLAGQLGSYRGGRSVLRYAYVAYAVLSIPSFMLMQQGSVNLAIAGLALGMIPYAL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
CQAGTYSVMPEFFPMHIRHTGVAFGHSVGAVIGGGSGPFLATWLIGATGNQSTPAFILCA
HCCCCHHCCHHHHHHHHHHCCHHHCCCCCCEEECCCCCEEEHHEEECCCCCCCCHHEEHH
SGLLGLIVISTVRKNAPVASDDVNTHQFS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNNNVMAEPVSLAVEETEQTRKAKKATTAAVFGTFVEYYDFSVYGYVAATLAMVFFPSD
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
NATIGLLNTFLVFGSAFLVRPLGAIAFGWLGDKVGRRFSLIASITLMGAAATLIGLLPGY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AQIGIWAPILLVVLRMLQGFSAGGEIGGAASYIREWAPPKRRALYISFLPSIAQFGKGLA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHEEHHHHHHHHHHHCCHHH
AAIAGLTAATLTDPEMLSWGWRIPFLLALPLGIIGLYMRLSIEDSPEFASKKNDAEVKKG
HHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHCCCCCCCCCCC
KPFAELIRDYRKPLAKVIMISMTQNIGTYIGTVFIAAYFSSILGYSKGEASTIVLIAVIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
AAFLIPLAGQLGSYRGGRSVLRYAYVAYAVLSIPSFMLMQQGSVNLAIAGLALGMIPYAL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
CQAGTYSVMPEFFPMHIRHTGVAFGHSVGAVIGGGSGPFLATWLIGATGNQSTPAFILCA
HCCCCHHCCHHHHHHHHHHCCHHHCCCCCCEEECCCCCEEEHHEEECCCCCCCCHHEEHH
SGLLGLIVISTVRKNAPVASDDVNTHQFS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]