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The map label for this gene is gyaR [H]

Identifier: 77459210

GI number: 77459210

Start: 3430139

End: 3431113

Strand: Direct

Name: gyaR [H]

Synonym: Pfl01_2987

Alternate gene names: 77459210

Gene position: 3430139-3431113 (Clockwise)

Preceding gene: 77459209

Following gene: 77459211

Centisome position: 53.28

GC content: 53.64

Gene sequence:

>975_bases
ATGTCTTTGATTTATGTGACTAGCCCGATCCATCCAGAAGTACTACAGGCCCTTTCGTCGGTGGGCGAAGTTCGACTCGG
CTATGGCCCAAATGCTGCGCCTTACTCGGAAATTCAAAACGAGGTAGACGCGGTATTTTTACGCGGCGGCCATATCTCCG
CCGAAATGATCGCGGCCTCACCTAAGCTTCGCATCGTAGCTCGTCATGGTGCGGGGTACGACAACGTCGACTACAAAGCA
GCAGCAGAGCTGGGGGTCTGGGTAACCAATACGCCTGGCGCGAACAGAAGAAGTGTTGTGGAGCATGTATTTGCTCTGCT
GCTGGGCATTAGCCGCAAGGTTCAACTGGCAACAGATCAAACCCGTAACAATATATGGGCGCAAGATCGACTGTCGCTTA
CAGGAATCGAGCTCGAAGGGCGCACCTTGGGCCTGATTGGGTTCGGCGACATTGGTCGGCATGTTGCGCCAGTTGCTGAA
GCTTTCGGAATGAAGGTTCTGGCGACTGATCCTGCTTATGACACGAGTTTTGATAAGCGTTTGGTAGATCTCGACACGTT
GTTGACCCAAGCAGACGTCGTGAGCCTCCACGTGCCTCTGCAGGAAGGCACCGAGAACCTGATCAGCAGAGCCGAAATCG
AGAAGATGAAAACCGGCGCTATCTTGATCAACACCAGTCGCGGTGGAGTGATCGATGAAGCAGCTGTTGCTGATGCCTTG
CGTAGCGGCAAGCTGGGTGGCGCGGGTATCGACGTTCTAGCTGCAGAAAACACGGATATGATCACCCCATTCAGTTACAA
CACATTCCCTGTTGCTGACCTTCCGAACCTACTCGTCACTCCCCACGTTGCCGGGCAAACAAATGAATCACTACTTCGGG
TTGGCATGAGCGCAGTAAAGGCTATTTCTGCTGTCTTGCGCGGCGCCCCCCCGCTCCACCCAGTCAACAACCCCGTACCT
AAAACATTTAACTAA

Upstream 100 bases:

>100_bases
TCAGGTCTATTGGGGCTGATCGTTATCTCGACCGTTCGTAAAAATGCGCCGGTAGCTAGCGATGACGTCAATACCCACCA
ATTCTCATAAGGTGACGATC

Downstream 100 bases:

>100_bases
TAGCGCTCTAAACAGCTTCCCGCCGAGGCCATCATGTTTATCAATGTATCTGATGAAAGCATCACTGTAAGCGAAGATTG
GGAACGCGCGTCCCTTGAAC

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSLIYVTSPIHPEVLQALSSVGEVRLGYGPNAAPYSEIQNEVDAVFLRGGHISAEMIAASPKLRIVARHGAGYDNVDYKA
AAELGVWVTNTPGANRRSVVEHVFALLLGISRKVQLATDQTRNNIWAQDRLSLTGIELEGRTLGLIGFGDIGRHVAPVAE
AFGMKVLATDPAYDTSFDKRLVDLDTLLTQADVVSLHVPLQEGTENLISRAEIEKMKTGAILINTSRGGVIDEAAVADAL
RSGKLGGAGIDVLAAENTDMITPFSYNTFPVADLPNLLVTPHVAGQTNESLLRVGMSAVKAISAVLRGAPPLHPVNNPVP
KTFN

Sequences:

>Translated_324_residues
MSLIYVTSPIHPEVLQALSSVGEVRLGYGPNAAPYSEIQNEVDAVFLRGGHISAEMIAASPKLRIVARHGAGYDNVDYKA
AAELGVWVTNTPGANRRSVVEHVFALLLGISRKVQLATDQTRNNIWAQDRLSLTGIELEGRTLGLIGFGDIGRHVAPVAE
AFGMKVLATDPAYDTSFDKRLVDLDTLLTQADVVSLHVPLQEGTENLISRAEIEKMKTGAILINTSRGGVIDEAAVADAL
RSGKLGGAGIDVLAAENTDMITPFSYNTFPVADLPNLLVTPHVAGQTNESLLRVGMSAVKAISAVLRGAPPLHPVNNPVP
KTFN
>Mature_323_residues
SLIYVTSPIHPEVLQALSSVGEVRLGYGPNAAPYSEIQNEVDAVFLRGGHISAEMIAASPKLRIVARHGAGYDNVDYKAA
AELGVWVTNTPGANRRSVVEHVFALLLGISRKVQLATDQTRNNIWAQDRLSLTGIELEGRTLGLIGFGDIGRHVAPVAEA
FGMKVLATDPAYDTSFDKRLVDLDTLLTQADVVSLHVPLQEGTENLISRAEIEKMKTGAILINTSRGGVIDEAAVADALR
SGKLGGAGIDVLAAENTDMITPFSYNTFPVADLPNLLVTPHVAGQTNESLLRVGMSAVKAISAVLRGAPPLHPVNNPVPK
TFN

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GyaR subfamily [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=300, Percent_Identity=36, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI4557499, Length=300, Percent_Identity=36, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI145580575, Length=256, Percent_Identity=38.28125, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI61743967, Length=288, Percent_Identity=33.6805555555556, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4557497, Length=288, Percent_Identity=33.6805555555556, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI23308577, Length=264, Percent_Identity=32.1969696969697, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI6912396, Length=320, Percent_Identity=28.125, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789279, Length=318, Percent_Identity=34.5911949685535, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1787645, Length=257, Percent_Identity=33.0739299610895, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082289, Length=280, Percent_Identity=31.0714285714286, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788660, Length=274, Percent_Identity=28.4671532846715, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=259, Percent_Identity=38.2239382239382, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25147481, Length=259, Percent_Identity=32.8185328185328, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322116, Length=286, Percent_Identity=30.4195804195804, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320925, Length=286, Percent_Identity=30.0699300699301, Blast_Score=135, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6324055, Length=216, Percent_Identity=33.7962962962963, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=262, Percent_Identity=30.9160305343511, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325144, Length=160, Percent_Identity=28.75, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324980, Length=170, Percent_Identity=30, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921140, Length=289, Percent_Identity=36.6782006920415, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646446, Length=272, Percent_Identity=35.6617647058824, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24646448, Length=272, Percent_Identity=35.6617647058824, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24646452, Length=272, Percent_Identity=35.6617647058824, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24646450, Length=272, Percent_Identity=35.6617647058824, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI62472511, Length=272, Percent_Identity=35.6617647058824, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28571528, Length=319, Percent_Identity=29.153605015674, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI28574286, Length=285, Percent_Identity=28.0701754385965, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574284, Length=296, Percent_Identity=30.4054054054054, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45551003, Length=296, Percent_Identity=30.4054054054054, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45552429, Length=296, Percent_Identity=30.4054054054054, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24585514, Length=296, Percent_Identity=30.4054054054054, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574282, Length=296, Percent_Identity=30.4054054054054, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24585516, Length=262, Percent_Identity=25.1908396946565, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.26 [H]

Molecular weight: Translated: 34469; Mature: 34338

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIYVTSPIHPEVLQALSSVGEVRLGYGPNAAPYSEIQNEVDAVFLRGGHISAEMIAAS
CEEEEEECCCCHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHEEEEECCCCEEEEEECC
PKLRIVARHGAGYDNVDYKAAAELGVWVTNTPGANRRSVVEHVFALLLGISRKVQLATDQ
CCEEEEEECCCCCCCCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
TRNNIWAQDRLSLTGIELEGRTLGLIGFGDIGRHVAPVAEAFGMKVLATDPAYDTSFDKR
CCCCCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHH
LVDLDTLLTQADVVSLHVPLQEGTENLISRAEIEKMKTGAILINTSRGGVIDEAAVADAL
HHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
RSGKLGGAGIDVLAAENTDMITPFSYNTFPVADLPNLLVTPHVAGQTNESLLRVGMSAVK
HCCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
AISAVLRGAPPLHPVNNPVPKTFN
HHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLIYVTSPIHPEVLQALSSVGEVRLGYGPNAAPYSEIQNEVDAVFLRGGHISAEMIAAS
EEEEEECCCCHHHHHHHHHHCCEEECCCCCCCCHHHHHHHHHEEEEECCCCEEEEEECC
PKLRIVARHGAGYDNVDYKAAAELGVWVTNTPGANRRSVVEHVFALLLGISRKVQLATDQ
CCEEEEEECCCCCCCCCHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCC
TRNNIWAQDRLSLTGIELEGRTLGLIGFGDIGRHVAPVAEAFGMKVLATDPAYDTSFDKR
CCCCCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHCCEEEEECCCCCCCHHHH
LVDLDTLLTQADVVSLHVPLQEGTENLISRAEIEKMKTGAILINTSRGGVIDEAAVADAL
HHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
RSGKLGGAGIDVLAAENTDMITPFSYNTFPVADLPNLLVTPHVAGQTNESLLRVGMSAVK
HCCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
AISAVLRGAPPLHPVNNPVPKTFN
HHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]