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The map label for this gene is alkB [H]

Identifier: 77459162

GI number: 77459162

Start: 3379062

End: 3379724

Strand: Direct

Name: alkB [H]

Synonym: Pfl01_2937

Alternate gene names: 77459162

Gene position: 3379062-3379724 (Clockwise)

Preceding gene: 77459161

Following gene: 77459163

Centisome position: 52.48

GC content: 62.29

Gene sequence:

>663_bases
ATGCAAACCACCTTCGACCTGTTCGCCGACACAGAATCCGAACAACCTCGGCGCGCCGAACAGATCGGCGAGCAATCCTG
GGTGCTGCGCGGCTTTGCCCTGCCGCAAATCGAGCAGCTATTGCCGGCCCTGGAATCGATCATCGCCAGCGCTCCGTTGC
GCCACATGATGACACCCGGAGGCTTCAGCATGTCGGTGGCCACCAGCAGTTGCGGCGCATTGGGCTGGATCACCGATCGA
AGCGGCTACCGCTATTCCTCCGAGGATCCCGTAAGCCATCGCCCCTGGCCGGCCATGCCCGAGGTGTTTCGCGAGCTGGC
CCAGGCAGCAGCGAAACGCGCCGGATTTGCCGACTTCATGCCGGACTCCTGCCTGATCAATCGCTATGTCCCAGGCGCGA
AGATGTCGTTGCATCAGGACAAAGACGAAAACGCCTACGAAGCCCCGATCGTTTCGCTGTCATTGGGCCTGCCGGCAACG
TTCGTGTTCGGCGGTTTCGCGCGCGGCGACAGGAGCCAGAAAATATCGCTGCTGCACGGCGACATGGTGATCTGGGGTGG
CGTTGACCGGCTGCGCTATCACGGCATCCTGCCGATCAAGCCTGGCCGGCACCCGCGCCTTGGCGAACAGCGTTTCAACC
TGACGTTTCGCACCGCTGGATAA

Upstream 100 bases:

>100_bases
GCGGCGACTATCGGGTGACCATGCGCCACGGCGTCAGCCGACTGCACAGCGGAACACGGCATACTCTGGGCATCATTTTC
CACGACGCGACCTGACGATC

Downstream 100 bases:

>100_bases
TCCTGCAAAGTTTGACCGCAAGGGTCGGAGTGTTCCGGCCGGTGGCACTGGTTAACCTGACATTCAACAGGTCAACGGAC
TCTCCATCATGAAAACGCTT

Product: DNA-N1-methyladenine dioxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MQTTFDLFADTESEQPRRAEQIGEQSWVLRGFALPQIEQLLPALESIIASAPLRHMMTPGGFSMSVATSSCGALGWITDR
SGYRYSSEDPVSHRPWPAMPEVFRELAQAAAKRAGFADFMPDSCLINRYVPGAKMSLHQDKDENAYEAPIVSLSLGLPAT
FVFGGFARGDRSQKISLLHGDMVIWGGVDRLRYHGILPIKPGRHPRLGEQRFNLTFRTAG

Sequences:

>Translated_220_residues
MQTTFDLFADTESEQPRRAEQIGEQSWVLRGFALPQIEQLLPALESIIASAPLRHMMTPGGFSMSVATSSCGALGWITDR
SGYRYSSEDPVSHRPWPAMPEVFRELAQAAAKRAGFADFMPDSCLINRYVPGAKMSLHQDKDENAYEAPIVSLSLGLPAT
FVFGGFARGDRSQKISLLHGDMVIWGGVDRLRYHGILPIKPGRHPRLGEQRFNLTFRTAG
>Mature_220_residues
MQTTFDLFADTESEQPRRAEQIGEQSWVLRGFALPQIEQLLPALESIIASAPLRHMMTPGGFSMSVATSSCGALGWITDR
SGYRYSSEDPVSHRPWPAMPEVFRELAQAAAKRAGFADFMPDSCLINRYVPGAKMSLHQDKDENAYEAPIVSLSLGLPAT
FVFGGFARGDRSQKISLLHGDMVIWGGVDRLRYHGILPIKPGRHPRLGEQRFNLTFRTAG

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Escherichia coli, GI1788541, Length=216, Percent_Identity=54.6296296296296, Blast_Score=243, Evalue=9e-66,
Organism=Drosophila melanogaster, GI45555401, Length=126, Percent_Identity=31.7460317460317, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24380; Mature: 24380

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTFDLFADTESEQPRRAEQIGEQSWVLRGFALPQIEQLLPALESIIASAPLRHMMTPG
CCCCCCCEECCCCCCCHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCC
GFSMSVATSSCGALGWITDRSGYRYSSEDPVSHRPWPAMPEVFRELAQAAAKRAGFADFM
CCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PDSCLINRYVPGAKMSLHQDKDENAYEAPIVSLSLGLPATFVFGGFARGDRSQKISLLHG
CCHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEECCCCHHHHHCCCCCCCCCCEEEEEEC
DMVIWGGVDRLRYHGILPIKPGRHPRLGEQRFNLTFRTAG
CEEEECCHHHHHHCCEEECCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MQTTFDLFADTESEQPRRAEQIGEQSWVLRGFALPQIEQLLPALESIIASAPLRHMMTPG
CCCCCCCEECCCCCCCHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCCC
GFSMSVATSSCGALGWITDRSGYRYSSEDPVSHRPWPAMPEVFRELAQAAAKRAGFADFM
CCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
PDSCLINRYVPGAKMSLHQDKDENAYEAPIVSLSLGLPATFVFGGFARGDRSQKISLLHG
CCHHHHHCCCCCCCCCCCCCCCCCCHHCCEEEEECCCCHHHHHCCCCCCCCCCEEEEEEC
DMVIWGGVDRLRYHGILPIKPGRHPRLGEQRFNLTFRTAG
CEEEECCHHHHHHCCEEECCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]