BacMap

Definition	Pseudomonas fluorescens Pf0-1 chromosome, complete genome.
Accession	NC_007492
Length	6,438,405

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The map label for this gene is fabG2 [H]

Identifier: 77459094

GI number: 77459094

Start: 3309501

End: 3310262

Strand: Reverse

Name: fabG2 [H]

Synonym: Pfl01_2869

Alternate gene names: 77459094

Gene position: 3310262-3309501 (Counterclockwise)

Preceding gene: 77459095

Following gene: 77459093

Centisome position: 51.41

GC content: 64.83

Gene sequence:

>762_bases
ATGCAGATCGAGAACAAGGTTTTTATCGTCACCGGCGGTGCGTCCGGGCTGGGTGCAGCCACCGCTGAGCTGTTGGTTGC
CGCCGGCGCCAAAGTGATGCTGGTGGACATGAACGCCGAAGCCGTCGCCGCTCAGGCCCAGCGCCTCGGCGCGCAAAGCG
TGGTGGCCGACATCAGCAACGAAGCCGCCGCCGAAGCCGCCGTGCAGGCGACCGTTAAAGCCTTCGGCAGCCTCAACGGG
CTGGTGAACTGCGCCGGCATCGTTCGCGGCGAGAAGATTCTTGGCAAGAACGGCCCGCACGCCTTGTCCAGCTTCGCCCA
GGTGATCAACGTCAACCTGATCGGCAGTTTCAACATGCTGCGTCTGGCAGCCGCTGCGATTGCCGAAAGCGAAGCCAACG
CCGACGGCGAGCGTGGTGTGATCATCAACACCGCGTCGGTTGCGGCGTTCGACGGCCAGATCGGCCAGGCCGCGTATTCG
GCGTCCAAAGGCGCGATCGCCAGCCTGACTCTGCCTGCCGCTCGTGAACTGGCGCGCTTCGGCATCCGCGTGATGACCAT
CGCCCCGGGCATTTTCGAAACCCCGATGATGGCCGGCATGACCCCGGAAGTGCGCGACTCGCTGGCCGCCGGCGTACCGT
TCCCGCCGCGTCTGGGCAAACCTGCCGAGTACGCCGCGCTGGTGCGGCATATCATTGAAAACAGCATGCTCAACGGCGAG
GTGATCCGTCTCGACGGTGCCTTGCGCATGGCTGCCAAGTAA

Upstream 100 bases:

>100_bases
AGCGGCAAATTGCAGCGCTTTATCTTGCGCAACCAGGAAATCGCCAAGGCTCAAGAGGCTGCGGCGCACAACGTTTCAGC
TTGAACCCAAGGAAACAGTC

Downstream 100 bases:

>100_bases
GGAGGATTTCCCATGACCATCTCCAACGATCCGATTGTCATCGTCAGCGCCGTCCGCACCCCGATGGGCGGTTTCCAGGG
CGAACTGAAAAGCCTGACCG

Product: Short-chain dehydrogenase/reductase SDR

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MQIENKVFIVTGGASGLGAATAELLVAAGAKVMLVDMNAEAVAAQAQRLGAQSVVADISNEAAAEAAVQATVKAFGSLNG
LVNCAGIVRGEKILGKNGPHALSSFAQVINVNLIGSFNMLRLAAAAIAESEANADGERGVIINTASVAAFDGQIGQAAYS
ASKGAIASLTLPAARELARFGIRVMTIAPGIFETPMMAGMTPEVRDSLAAGVPFPPRLGKPAEYAALVRHIIENSMLNGE
VIRLDGALRMAAK

Sequences:

>Translated_253_residues
MQIENKVFIVTGGASGLGAATAELLVAAGAKVMLVDMNAEAVAAQAQRLGAQSVVADISNEAAAEAAVQATVKAFGSLNG
LVNCAGIVRGEKILGKNGPHALSSFAQVINVNLIGSFNMLRLAAAAIAESEANADGERGVIINTASVAAFDGQIGQAAYS
ASKGAIASLTLPAARELARFGIRVMTIAPGIFETPMMAGMTPEVRDSLAAGVPFPPRLGKPAEYAALVRHIIENSMLNGE
VIRLDGALRMAAK
>Mature_253_residues
MQIENKVFIVTGGASGLGAATAELLVAAGAKVMLVDMNAEAVAAQAQRLGAQSVVADISNEAAAEAAVQATVKAFGSLNG
LVNCAGIVRGEKILGKNGPHALSSFAQVINVNLIGSFNMLRLAAAAIAESEANADGERGVIINTASVAAFDGQIGQAAYS
ASKGAIASLTLPAARELARFGIRVMTIAPGIFETPMMAGMTPEVRDSLAAGVPFPPRLGKPAEYAALVRHIIENSMLNGE
VIRLDGALRMAAK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=252, Percent_Identity=53.5714285714286, Blast_Score=253, Evalue=9e-68,
Organism=Homo sapiens, GI83715985, Length=252, Percent_Identity=50, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI40254992, Length=248, Percent_Identity=29.8387096774194, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI15277342, Length=269, Percent_Identity=30.8550185873606, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI59889578, Length=210, Percent_Identity=30, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI31542939, Length=261, Percent_Identity=29.5019157088123, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI224922801, Length=181, Percent_Identity=32.5966850828729, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI10190704, Length=194, Percent_Identity=26.8041237113402, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI19923817, Length=264, Percent_Identity=28.030303030303, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=31.496062992126, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI87082160, Length=258, Percent_Identity=29.8449612403101, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790717, Length=260, Percent_Identity=30, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789208, Length=261, Percent_Identity=29.5019157088123, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=28.735632183908, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786812, Length=196, Percent_Identity=32.1428571428571, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367175, Length=249, Percent_Identity=32.9317269076305, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787905, Length=258, Percent_Identity=29.4573643410853, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788459, Length=254, Percent_Identity=27.1653543307087, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789057, Length=212, Percent_Identity=29.2452830188679, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=248, Percent_Identity=49.5967741935484, Blast_Score=231, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=33.8582677165354, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25147288, Length=251, Percent_Identity=30.2788844621514, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17563726, Length=247, Percent_Identity=28.7449392712551, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=273, Percent_Identity=29.6703296703297, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559104, Length=266, Percent_Identity=27.4436090225564, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=271, Percent_Identity=27.3062730627306, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17562904, Length=208, Percent_Identity=27.8846153846154, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=218, Percent_Identity=31.1926605504587, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565030, Length=231, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17551412, Length=208, Percent_Identity=31.7307692307692, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=26.5625, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=269, Percent_Identity=31.2267657992565, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=255, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=195, Percent_Identity=29.2307692307692, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=195, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=274, Percent_Identity=27.007299270073, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=207, Percent_Identity=30.4347826086957, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17560676, Length=262, Percent_Identity=26.7175572519084, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17538486, Length=204, Percent_Identity=27.9411764705882, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17564282, Length=210, Percent_Identity=29.5238095238095, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=202, Percent_Identity=27.7227722772277, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=201, Percent_Identity=30.8457711442786, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17737361, Length=252, Percent_Identity=53.968253968254, Blast_Score=259, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=35.2, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21357041, Length=229, Percent_Identity=30.1310043668122, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24644339, Length=232, Percent_Identity=31.4655172413793, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI23397609, Length=199, Percent_Identity=35.1758793969849, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571526, Length=229, Percent_Identity=29.2576419213974, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24665243, Length=218, Percent_Identity=29.3577981651376, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI221331218, Length=260, Percent_Identity=28.0769230769231, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665247, Length=219, Percent_Identity=29.6803652968037, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221331216, Length=261, Percent_Identity=28.3524904214559, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571387, Length=209, Percent_Identity=27.7511961722488, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 25766; Mature: 25766

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIENKVFIVTGGASGLGAATAELLVAAGAKVMLVDMNAEAVAAQAQRLGAQSVVADISN
CCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHH
EAAAEAAVQATVKAFGSLNGLVNCAGIVRGEKILGKNGPHALSSFAQVINVNLIGSFNML
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHEEEECCHHHH
RLAAAAIAESEANADGERGVIINTASVAAFDGQIGQAAYSASKGAIASLTLPAARELARF
HHHHHHHHHHCCCCCCCCCEEEEECEEEEECCCHHHHHHHCCCCCEEEEECHHHHHHHHC
GIRVMTIAPGIFETPMMAGMTPEVRDSLAAGVPFPPRLGKPAEYAALVRHIIENSMLNGE
CEEEEEECCCHHHCCHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
VIRLDGALRMAAK
EEEECCCCCCCCC
>Mature Secondary Structure
MQIENKVFIVTGGASGLGAATAELLVAAGAKVMLVDMNAEAVAAQAQRLGAQSVVADISN
CCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHH
EAAAEAAVQATVKAFGSLNGLVNCAGIVRGEKILGKNGPHALSSFAQVINVNLIGSFNML
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHEEEECCHHHH
RLAAAAIAESEANADGERGVIINTASVAAFDGQIGQAAYSASKGAIASLTLPAARELARF
HHHHHHHHHHCCCCCCCCCEEEEECEEEEECCCHHHHHHHCCCCCEEEEECHHHHHHHHC
GIRVMTIAPGIFETPMMAGMTPEVRDSLAAGVPFPPRLGKPAEYAALVRHIIENSMLNGE
CEEEEEECCCHHHCCHHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCE
VIRLDGALRMAAK
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]