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The map label for this gene is thlA [H]

Identifier: 77459093

GI number: 77459093

Start: 3308298

End: 3309488

Strand: Reverse

Name: thlA [H]

Synonym: Pfl01_2868

Alternate gene names: 77459093

Gene position: 3309488-3308298 (Counterclockwise)

Preceding gene: 77459094

Following gene: 77459092

Centisome position: 51.4

GC content: 64.15

Gene sequence:

>1191_bases
ATGACCATCTCCAACGATCCGATTGTCATCGTCAGCGCCGTCCGCACCCCGATGGGCGGTTTCCAGGGCGAACTGAAAAG
CCTGACCGCGCCGCAACTGGGCGCCGCTGCAATCAAGGCGGCGGTTGAGCGTGCCGGTGTGGCCGCCGATGCGGTCGATG
AAGTGTTGTTCGGCTGCGTGTTGCCGGCCGGTCTTGGTCAGGCGCCTGCACGTCAGGCTGCACTGGGCGCCGGGCTGGAC
AAGTCCACCCGCTGCACCACGGTCAACAAGATGTGCGGTTCGGGCATGGAAACCGCCATTCTGGCCCACGACATGCTGCT
GGCTGGCAGCGCCGATGTGGTGATCGCCGGCGGCATGGAAAGCATGTCCAACGCGCCGTATCTGCTGGATCGCGCCCGTG
CCGGTTACCGCATGGGCCACGGCCGGGTGCTGGATTCGATGTTCCTCGACGGCCTCGAAGACGCCTATGACAAGGGCCGC
CTGATGGGCACCTTCGCCGAGGATTGCGCCGAAACCAACGACTTCAGCCGTGAAGCGCAGGACGCCTTTGCCATCGCCTC
GACCACCCGCGCCCAGCAGGCGATCAAGGACGGCAGCTTCAAGGACGAAATCGTTCCGCTGACCGTGACCGTCGGCAAGG
AACAGGTTGTGATCAGCAACGATGAGCAGCCACCAAAAGCCAAGCTGGACAAGATTGCTTCGCTTAAACCGGCGTTCCGC
GAAGGCGGCACCGTGACCGCTGCCAACTCCAGCTCAATCTCTGACGGCGCCGCTGCGCTGGTGCTGATGCGTCAGTCGCA
AGCACAAAAGCAGGGTCTGAAGCCGCTGGCGGTGATTCACGGACACGCGGCGTTTGCCGACACCCCGGGCCTGTTCCCGG
TGGCACCGATTGGCGCGATCAAGAAGCTGATGAAGAAAACCGGCTGGTCGCTGGATCAGGTCGATCTGTTCGAAGTCAAC
GAAGCCTTCGCCGTGGTCGGCATGGCGGCGATGACCCACCTGGAAATCCCTCACGACAAGCTCAACATCCATGGCGGCGC
CTGCGCCCTCGGTCACCCGATCGGTGCGTCCGGCGCGCGGATTCTGGTGACTTTGCTCTCGGCCCTGCGCCAGAAAGGCC
TGAAACGCGGCGTCGCGGCGATCTGCATCGGCGGCGGCGAAGCCACGGCGATGGCTGTCGAGTGCGTCTGA

Upstream 100 bases:

>100_bases
CGCGCTGGTGCGGCATATCATTGAAAACAGCATGCTCAACGGCGAGGTGATCCGTCTCGACGGTGCCTTGCGCATGGCTG
CCAAGTAAGGAGGATTTCCC

Downstream 100 bases:

>100_bases
ACCCGAGTTAAGGATTTGCCATGATTCCCAATGACGACCAACAACAAATCCGCGACATGGCCCGGCAGTTTGCCGAGGAA
CGGCTCAAACCGTTTGCCGC

Product: thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTISNDPIVIVSAVRTPMGGFQGELKSLTAPQLGAAAIKAAVERAGVAADAVDEVLFGCVLPAGLGQAPARQAALGAGLD
KSTRCTTVNKMCGSGMETAILAHDMLLAGSADVVIAGGMESMSNAPYLLDRARAGYRMGHGRVLDSMFLDGLEDAYDKGR
LMGTFAEDCAETNDFSREAQDAFAIASTTRAQQAIKDGSFKDEIVPLTVTVGKEQVVISNDEQPPKAKLDKIASLKPAFR
EGGTVTAANSSSISDGAAALVLMRQSQAQKQGLKPLAVIHGHAAFADTPGLFPVAPIGAIKKLMKKTGWSLDQVDLFEVN
EAFAVVGMAAMTHLEIPHDKLNIHGGACALGHPIGASGARILVTLLSALRQKGLKRGVAAICIGGGEATAMAVECV

Sequences:

>Translated_396_residues
MTISNDPIVIVSAVRTPMGGFQGELKSLTAPQLGAAAIKAAVERAGVAADAVDEVLFGCVLPAGLGQAPARQAALGAGLD
KSTRCTTVNKMCGSGMETAILAHDMLLAGSADVVIAGGMESMSNAPYLLDRARAGYRMGHGRVLDSMFLDGLEDAYDKGR
LMGTFAEDCAETNDFSREAQDAFAIASTTRAQQAIKDGSFKDEIVPLTVTVGKEQVVISNDEQPPKAKLDKIASLKPAFR
EGGTVTAANSSSISDGAAALVLMRQSQAQKQGLKPLAVIHGHAAFADTPGLFPVAPIGAIKKLMKKTGWSLDQVDLFEVN
EAFAVVGMAAMTHLEIPHDKLNIHGGACALGHPIGASGARILVTLLSALRQKGLKRGVAAICIGGGEATAMAVECV
>Mature_395_residues
TISNDPIVIVSAVRTPMGGFQGELKSLTAPQLGAAAIKAAVERAGVAADAVDEVLFGCVLPAGLGQAPARQAALGAGLDK
STRCTTVNKMCGSGMETAILAHDMLLAGSADVVIAGGMESMSNAPYLLDRARAGYRMGHGRVLDSMFLDGLEDAYDKGRL
MGTFAEDCAETNDFSREAQDAFAIASTTRAQQAIKDGSFKDEIVPLTVTVGKEQVVISNDEQPPKAKLDKIASLKPAFRE
GGTVTAANSSSISDGAAALVLMRQSQAQKQGLKPLAVIHGHAAFADTPGLFPVAPIGAIKKLMKKTGWSLDQVDLFEVNE
AFAVVGMAAMTHLEIPHDKLNIHGGACALGHPIGASGARILVTLLSALRQKGLKRGVAAICIGGGEATAMAVECV

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=47.3007712082262, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=46.3476070528967, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=36.7609254498715, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=35.8395989974937, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI4504327, Length=422, Percent_Identity=31.9905213270142, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=28.4263959390863, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=44.3298969072165, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=49.2268041237113, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1787663, Length=397, Percent_Identity=35.5163727959698, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=36.9458128078818, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=30.5623471882641, Blast_Score=164, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI25147385, Length=392, Percent_Identity=42.8571428571429, Blast_Score=301, Evalue=4e-82,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=42.3857868020305, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=38.974358974359, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17535917, Length=396, Percent_Identity=36.1111111111111, Blast_Score=204, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=32.7790973871734, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325229, Length=395, Percent_Identity=48.1012658227848, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6322031, Length=387, Percent_Identity=33.0749354005168, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=48.469387755102, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=45.1030927835052, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=35.0877192982456, Blast_Score=231, Evalue=5e-61,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=30.8788598574822, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40935; Mature: 40803

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISNDPIVIVSAVRTPMGGFQGELKSLTAPQLGAAAIKAAVERAGVAADAVDEVLFGCV
CCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LPAGLGQAPARQAALGAGLDKSTRCTTVNKMCGSGMETAILAHDMLLAGSADVVIAGGME
HCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHEECCCCEEEECCHH
SMSNAPYLLDRARAGYRMGHGRVLDSMFLDGLEDAYDKGRLMGTFAEDCAETNDFSREAQ
HCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCHHHHHHH
DAFAIASTTRAQQAIKDGSFKDEIVPLTVTVGKEQVVISNDEQPPKAKLDKIASLKPAFR
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCCHHHHHHHHHCCHHHH
EGGTVTAANSSSISDGAAALVLMRQSQAQKQGLKPLAVIHGHAAFADTPGLFPVAPIGAI
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCCCCCCCHHHH
KKLMKKTGWSLDQVDLFEVNEAFAVVGMAAMTHLEIPHDKLNIHGGACALGHPIGASGAR
HHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEECCCCCCCCHHH
ILVTLLSALRQKGLKRGVAAICIGGGEATAMAVECV
HHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TISNDPIVIVSAVRTPMGGFQGELKSLTAPQLGAAAIKAAVERAGVAADAVDEVLFGCV
CCCCCCEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LPAGLGQAPARQAALGAGLDKSTRCTTVNKMCGSGMETAILAHDMLLAGSADVVIAGGME
HCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHEECCCCEEEECCHH
SMSNAPYLLDRARAGYRMGHGRVLDSMFLDGLEDAYDKGRLMGTFAEDCAETNDFSREAQ
HCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHCCCHHHHHHH
DAFAIASTTRAQQAIKDGSFKDEIVPLTVTVGKEQVVISNDEQPPKAKLDKIASLKPAFR
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCCCHHHHHHHHHCCHHHH
EGGTVTAANSSSISDGAAALVLMRQSQAQKQGLKPLAVIHGHAAFADTPGLFPVAPIGAI
CCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHCCCCCCCCCCCCHHHH
KKLMKKTGWSLDQVDLFEVNEAFAVVGMAAMTHLEIPHDKLNIHGGACALGHPIGASGAR
HHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEECCCCCCCCHHH
ILVTLLSALRQKGLKRGVAAICIGGGEATAMAVECV
HHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]