Click here to switch to the map view.

The map label for this gene is phaB [H]

Identifier: 77459011

GI number: 77459011

Start: 3212166

End: 3212966

Strand: Reverse

Name: phaB [H]

Synonym: Pfl01_2786

Alternate gene names: 77459011

Gene position: 3212966-3212166 (Counterclockwise)

Preceding gene: 77459012

Following gene: 77459007

Centisome position: 49.9

GC content: 55.68

Gene sequence:

>801_bases
ATGACAACGCACAGGCTAACAACGAAGGGCTTGCAGTTGAACGTGTCGATCTGCGGTCAAGGCTCTCCGCTGCTTTTGCT
CAATGGTTTGGGTGGTTTGATTCGTACCTTCGATCCGCTGCGCAATGAACTTGACGACTTCATGACCATCACGCTGGACG
TGCCCGGAGTCGGCAAGTCGCAAATGCCCCGTTGGCCCTTTCGCCTGCCACGCCATACCGACGTGATTGCCGGGATGCTT
GAGCAATTGGGGATCGACCAGGTCGATGTGTTCGGTGTGAGTTGGGGAGGTGCATTGGCTCAGGAGTTCGCTCTTCGTTA
CCCGAGCAGGGTACGCAGGTTGATCCTCGCGGCGACCTCTCCCGGCCCATTGCTGTTGATGAAGCCTGCAGACGTTATGG
ATTTTTTCCGCAAGAGCAAAAACACCAAAACGCACCAGCAAGAACGATCCGGCCATTCAGTTCAGACGCTGCTGCGGTTC
GGTGTAATGAAGGGCATGCTCTCTGTTAATCCGCGAACCTACTATTTCCAGCTCGCAGCCTTGATCGGATGGACAAGCCT
GCTTCGCTTGTCCCGACTGCTTCAACGCACGCTGATTCTTGCCGGCGACCGCGATCCGTTAATACAGGCGTACAACGGCC
ATATCCTGCGCCGCACAATTGGACGTTCAGAACTTCGCCTTCTGAAGGGTGAGGGACATTTCTTTGTGGTGACCAGCGCT
GGACGAACTGCCGTCCTGGTCCGCGAATTTCTTTGCCGCCATTACGACGATGAAGAGAAGGCACCGAGGATCGCGAACTG
A

Upstream 100 bases:

>100_bases
ATTGGACGGCGTGGTTGAAGAAACTGGAAGCAAAAATCCGACGGTAGGCGTCGCGATCCCACCTGCACTGCCTTTCAATT
ACGTGCAGCAAGGAGAAATC

Downstream 100 bases:

>100_bases
AACACCAAGGCTGGCCTGGAGCGCACCCAGGTCAGTTAATCCGCTCTTCTCATCAATAGTCGCTCTGCACCTGATAAAGA
AAAGGGGAGACCCGCTATCA

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTTHRLTTKGLQLNVSICGQGSPLLLLNGLGGLIRTFDPLRNELDDFMTITLDVPGVGKSQMPRWPFRLPRHTDVIAGML
EQLGIDQVDVFGVSWGGALAQEFALRYPSRVRRLILAATSPGPLLLMKPADVMDFFRKSKNTKTHQQERSGHSVQTLLRF
GVMKGMLSVNPRTYYFQLAALIGWTSLLRLSRLLQRTLILAGDRDPLIQAYNGHILRRTIGRSELRLLKGEGHFFVVTSA
GRTAVLVREFLCRHYDDEEKAPRIAN

Sequences:

>Translated_266_residues
MTTHRLTTKGLQLNVSICGQGSPLLLLNGLGGLIRTFDPLRNELDDFMTITLDVPGVGKSQMPRWPFRLPRHTDVIAGML
EQLGIDQVDVFGVSWGGALAQEFALRYPSRVRRLILAATSPGPLLLMKPADVMDFFRKSKNTKTHQQERSGHSVQTLLRF
GVMKGMLSVNPRTYYFQLAALIGWTSLLRLSRLLQRTLILAGDRDPLIQAYNGHILRRTIGRSELRLLKGEGHFFVVTSA
GRTAVLVREFLCRHYDDEEKAPRIAN
>Mature_265_residues
TTHRLTTKGLQLNVSICGQGSPLLLLNGLGGLIRTFDPLRNELDDFMTITLDVPGVGKSQMPRWPFRLPRHTDVIAGMLE
QLGIDQVDVFGVSWGGALAQEFALRYPSRVRRLILAATSPGPLLLMKPADVMDFFRKSKNTKTHQQERSGHSVQTLLRFG
VMKGMLSVNPRTYYFQLAALIGWTSLLRLSRLLQRTLILAGDRDPLIQAYNGHILRRTIGRSELRLLKGEGHFFVVTSAG
RTAVLVREFLCRHYDDEEKAPRIAN

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 29904; Mature: 29773

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHRLTTKGLQLNVSICGQGSPLLLLNGLGGLIRTFDPLRNELDDFMTITLDVPGVGKS
CCCCCEEECCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEEECCCCCCC
QMPRWPFRLPRHTDVIAGMLEQLGIDQVDVFGVSWGGALAQEFALRYPSRVRRLILAATS
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCHHHHHHEEEEECC
PGPLLLMKPADVMDFFRKSKNTKTHQQERSGHSVQTLLRFGVMKGMLSVNPRTYYFQLAA
CCCEEEECCHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LIGWTSLLRLSRLLQRTLILAGDRDPLIQAYNGHILRRTIGRSELRLLKGEGHFFVVTSA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEHHHCHHHEEEEECCCCEEEEECC
GRTAVLVREFLCRHYDDEEKAPRIAN
CCHHHHHHHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure 
TTHRLTTKGLQLNVSICGQGSPLLLLNGLGGLIRTFDPLRNELDDFMTITLDVPGVGKS
CCCCEEECCEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEEECCCCCCC
QMPRWPFRLPRHTDVIAGMLEQLGIDQVDVFGVSWGGALAQEFALRYPSRVRRLILAATS
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCHHHHHHEEEEECC
PGPLLLMKPADVMDFFRKSKNTKTHQQERSGHSVQTLLRFGVMKGMLSVNPRTYYFQLAA
CCCEEEECCHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LIGWTSLLRLSRLLQRTLILAGDRDPLIQAYNGHILRRTIGRSELRLLKGEGHFFVVTSA
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEHHHCHHHEEEEECCCCEEEEECC
GRTAVLVREFLCRHYDDEEKAPRIAN
CCHHHHHHHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]