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The map label for this gene is nemA [H]

Identifier: 77458704

GI number: 77458704

Start: 2834065

End: 2835165

Strand: Reverse

Name: nemA [H]

Synonym: Pfl01_2479

Alternate gene names: 77458704

Gene position: 2835165-2834065 (Counterclockwise)

Preceding gene: 77458708

Following gene: 77458703

Centisome position: 44.04

GC content: 62.49

Gene sequence:

>1101_bases
ATGACCAAGCAGACTCTTTTCTCCCCTTACACGCTAGGCAGTCTCACGCTGTCGAACCGCGTCGTTCTTGCACCGCTGAC
ACGCAACCGCGCAGGCCAGGGCTTTGTCCCCAGCGAATTCGCGGCCGTCTATTACAGCCAGCGCGCCAGCGCCGGTTTGC
TGATCAGCGAAGCGACGCAGATTTCCCAACAGGGTCAGGGTTATCAGGACACCCCCGGGATCTACACACAGGCACAGATT
GACGGTTGGCGCACAGTCACTGATGCCGTCCACGCCAAAGGCGGAAAAATCTTCCTGCAGCTGTGGCATGTCGGCCGCGT
TTCACACGTTGACCTGCAAGAAAACGGCGCCGCACCGGTGGCCCCTTCTGCGCTGCGTGCCGCGACCAAAGTGTTCGTCA
ACAACCGCTTTGAAGACGTCTCCGAACCCCGCGCCCTGGAAACTAGCGAACTGCCGGGGATCGTCTCCGATTTCCGCCAG
GCTGCGGCCAACGCAGTCGCCGCCGGGTTCGATGGCGTGGAAATTCACGGTGCAAACGGCTATTTGCTGGATCAATTCCT
CAGGGACAACGCCAATCTGCGCACCGACGCTTACGGCGGCTCGATTGAAAATCGTGCGCGTCTGTTGCTCGAAGTGACAG
CGGCTGTCATCAACGAGATCGGCGCGGATCGCACCGGTGTGCGCCTGTCTCCCGTGTCGCCGGCCAATGGCGTCTCCAGC
AGCGATCCGCAGGCGCAATTCAATTACATCGTCGAGCAACTCGACGCCCTCGGTGTGGTTTATCTGCACATGGTCGAAGG
CGCGACCGGCGGCCCGCGCGACGTGGCCCCTTTCGATTTCGGCGCCCTGCGCCAGCGCTTCAAAAACACCTATATCGCCA
ACAACGGCTATGACCTGGACCTGGCGGCTGCGCGGCTGGCCGAAGACCAGGCCGATCTGATCGCCTTCGGTCGCCCGTTC
ATTGCCAACCCGGATCTGGTGGAGCGCCTCAAGCAGGGCGCGCCGCTGTCCGCGTTCAATCCCGCCACCCTCTACGGCGG
CGGCGCGGCGGGCTACATCGACTACCCGACGCTGGCTGAATCGAGCGTCAGCGCAAGCTGA

Upstream 100 bases:

>100_bases
TGTTGGCCATAATCAAAACAGTTGACAGCTTAGATTTCGATCATCATCCTAATTTCACATCCCCACCGATCTTCATCCAC
GGGCACGGAAGATTCATCAG

Downstream 100 bases:

>100_bases
GCGCGTTCTGTTTCTCACCTCCTGAAAGAGAGATACCCCATGAGCACTCGCCCTACTGTTCTCATCACTGGCGCCTCTAC
CGGCATTGGCGCCGTCTATG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTKQTLFSPYTLGSLTLSNRVVLAPLTRNRAGQGFVPSEFAAVYYSQRASAGLLISEATQISQQGQGYQDTPGIYTQAQI
DGWRTVTDAVHAKGGKIFLQLWHVGRVSHVDLQENGAAPVAPSALRAATKVFVNNRFEDVSEPRALETSELPGIVSDFRQ
AAANAVAAGFDGVEIHGANGYLLDQFLRDNANLRTDAYGGSIENRARLLLEVTAAVINEIGADRTGVRLSPVSPANGVSS
SDPQAQFNYIVEQLDALGVVYLHMVEGATGGPRDVAPFDFGALRQRFKNTYIANNGYDLDLAAARLAEDQADLIAFGRPF
IANPDLVERLKQGAPLSAFNPATLYGGGAAGYIDYPTLAESSVSAS

Sequences:

>Translated_366_residues
MTKQTLFSPYTLGSLTLSNRVVLAPLTRNRAGQGFVPSEFAAVYYSQRASAGLLISEATQISQQGQGYQDTPGIYTQAQI
DGWRTVTDAVHAKGGKIFLQLWHVGRVSHVDLQENGAAPVAPSALRAATKVFVNNRFEDVSEPRALETSELPGIVSDFRQ
AAANAVAAGFDGVEIHGANGYLLDQFLRDNANLRTDAYGGSIENRARLLLEVTAAVINEIGADRTGVRLSPVSPANGVSS
SDPQAQFNYIVEQLDALGVVYLHMVEGATGGPRDVAPFDFGALRQRFKNTYIANNGYDLDLAAARLAEDQADLIAFGRPF
IANPDLVERLKQGAPLSAFNPATLYGGGAAGYIDYPTLAESSVSAS
>Mature_365_residues
TKQTLFSPYTLGSLTLSNRVVLAPLTRNRAGQGFVPSEFAAVYYSQRASAGLLISEATQISQQGQGYQDTPGIYTQAQID
GWRTVTDAVHAKGGKIFLQLWHVGRVSHVDLQENGAAPVAPSALRAATKVFVNNRFEDVSEPRALETSELPGIVSDFRQA
AANAVAAGFDGVEIHGANGYLLDQFLRDNANLRTDAYGGSIENRARLLLEVTAAVINEIGADRTGVRLSPVSPANGVSSS
DPQAQFNYIVEQLDALGVVYLHMVEGATGGPRDVAPFDFGALRQRFKNTYIANNGYDLDLAAARLAEDQADLIAFGRPFI
ANPDLVERLKQGAPLSAFNPATLYGGGAAGYIDYPTLAESSVSAS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=366, Percent_Identity=49.1803278688525, Blast_Score=334, Evalue=5e-93,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=30.2593659942363, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=260, Percent_Identity=30.3846153846154, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=267, Percent_Identity=29.9625468164794, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=263, Percent_Identity=30.0380228136882, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=288, Percent_Identity=27.4305555555556, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI72001454, Length=259, Percent_Identity=30.5019305019305, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=221, Percent_Identity=33.0316742081448, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17566914, Length=90, Percent_Identity=42.2222222222222, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=385, Percent_Identity=37.9220779220779, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=37.2750642673522, Blast_Score=222, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39040; Mature: 38909

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQTLFSPYTLGSLTLSNRVVLAPLTRNRAGQGFVPSEFAAVYYSQRASAGLLISEATQ
CCCCCCCCCCEECEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHH
ISQQGQGYQDTPGIYTQAQIDGWRTVTDAVHAKGGKIFLQLWHVGRVSHVDLQENGAAPV
HHHCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCEEEEEHHCCCCCCC
APSALRAATKVFVNNRFEDVSEPRALETSELPGIVSDFRQAAANAVAAGFDGVEIHGANG
CHHHHHHHHHHHHCCCHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLLDQFLRDNANLRTDAYGGSIENRARLLLEVTAAVINEIGADRTGVRLSPVSPANGVSS
HHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
SDPQAQFNYIVEQLDALGVVYLHMVEGATGGPRDVAPFDFGALRQRFKNTYIANNGYDLD
CCCHHHHHHHHHHHHHHHHEEHHEECCCCCCCCCCCCCCHHHHHHHHHHCEECCCCCCHH
LAAARLAEDQADLIAFGRPFIANPDLVERLKQGAPLSAFNPATLYGGGAAGYIDYPTLAE
HHHHHHHCCCHHHEEECCCCCCCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCC
SSVSAS
CCCCCC
>Mature Secondary Structure 
TKQTLFSPYTLGSLTLSNRVVLAPLTRNRAGQGFVPSEFAAVYYSQRASAGLLISEATQ
CCCCCCCCCEECEEEECCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHH
ISQQGQGYQDTPGIYTQAQIDGWRTVTDAVHAKGGKIFLQLWHVGRVSHVDLQENGAAPV
HHHCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCEEEEEHHCCCCCCC
APSALRAATKVFVNNRFEDVSEPRALETSELPGIVSDFRQAAANAVAAGFDGVEIHGANG
CHHHHHHHHHHHHCCCHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCC
YLLDQFLRDNANLRTDAYGGSIENRARLLLEVTAAVINEIGADRTGVRLSPVSPANGVSS
HHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCC
SDPQAQFNYIVEQLDALGVVYLHMVEGATGGPRDVAPFDFGALRQRFKNTYIANNGYDLD
CCCHHHHHHHHHHHHHHHHEEHHEECCCCCCCCCCCCCCHHHHHHHHHHCEECCCCCCHH
LAAARLAEDQADLIAFGRPFIANPDLVERLKQGAPLSAFNPATLYGGGAAGYIDYPTLAE
HHHHHHHCCCHHHEEECCCCCCCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCC
SSVSAS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]