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The map label for this gene is potF [H]

Identifier: 77458599

GI number: 77458599

Start: 2730638

End: 2731741

Strand: Direct

Name: potF [H]

Synonym: Pfl01_2372

Alternate gene names: 77458599

Gene position: 2730638-2731741 (Clockwise)

Preceding gene: 77458598

Following gene: 77458600

Centisome position: 42.41

GC content: 61.41

Gene sequence:

>1104_bases
ATGCCTACTTCACACAAGTGGTTGCTGGGCGCTCTGGGCCTGGCGCTGGCCGGCCTGATGCCGGCCGTGCACGCCGAAGA
CAAGACCTTACGGCTGTACAACTGGGCGGATTATTTCGCTGAAGACACACTGAGCAAATTCACCGCCGAAACCGGAATCA
AGGTGATCTACGACGTGATGGACGGCAGCGAAACCCTGGAAGCCAAAATGCTCTCCGGCGGCAGCGGCTACGATCTGATC
TTCCCCGGTGACACCGTGGCCGAACGGCTGATGCGCGCCGGCAGCCTGTTGCCGCTGGATCCTTCGAAGATCACCGCGAT
GAACGACATCGAACCGGGCCTGCAAAAGCTGCGCAGCCATTACGAGCATTCGAACAAGGCCACCGTGCCGTACACCTGGG
GCACCATCGGCCTGACCTACAACGCCGAGCAGATCCAGCAACGCATGGCCGACGCGCCGGTCAACAGCCTCGACATGCTG
TTCAAACCGGAACTGGCCGCGAAGTTTGCCGATTGCGGGATTTCGATGATCGACTCGCCGGACGAAGTGCTGGCCGTGGT
GCTCAACTATCTGGGCCGCGACCCGCGCAGCGCCAAGCCTGCGGATCTGGCGGCGGCCAGTGATTTGCTGATGAAGCTGC
GGCCGTACATTCGCAAGTTCCAGTCGCAACCGGTGACCGATCTGGTCAACGGCAACCTGTGCCTGTCCCTCGGCTACAGC
GGCGACATGACTCAGGCCCAGCGCACTTCGGACAGCGCCGGCAAGCAGACCCGCTTCCAGTACCGCGTGCCGCGTGAGGG
CACCACGGTGTGGATGGACACCATGGCCATTCCGGTGGATGCCAAACACCCGGAATACGCCTACGAATTCATCAACTTCG
TGATGCGCCCGGAAAACATGGCTGCGATCAGCAATTTCACCGGTTACCCGACCTCCAACGCCAAGGCCCGGCCAAACGTG
GATGAGGCCATGCGCAACAACCCGGACATCTACCTCGACGAAGCGACCTATGCTCGTCTGATACCGGGCAAGGACATTCC
CCAGGCCGACATGCGCGCCCGCATGCGCACCTGGACCAAGTTCAAGACTGCCACTGCCAAGTGA

Upstream 100 bases:

>100_bases
TGGGGCATCTACCGCGACCGTCGTCCGGAAATGTATGGCGCTTTGCTCAGCCAGGACGGCCGTCACCTTAACGTCCGCTG
GAATCCCCAAGGAGTCTGAT

Downstream 100 bases:

>100_bases
TTTTTCCGGGCCGCCCCGGCGGCCCCTTACCCATCAGGAACGTACACATGCCGACTCGTCGCGAGTTCATCAAACAGGTT
TCAGTCGCCGTCGGTGCAGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MPTSHKWLLGALGLALAGLMPAVHAEDKTLRLYNWADYFAEDTLSKFTAETGIKVIYDVMDGSETLEAKMLSGGSGYDLI
FPGDTVAERLMRAGSLLPLDPSKITAMNDIEPGLQKLRSHYEHSNKATVPYTWGTIGLTYNAEQIQQRMADAPVNSLDML
FKPELAAKFADCGISMIDSPDEVLAVVLNYLGRDPRSAKPADLAAASDLLMKLRPYIRKFQSQPVTDLVNGNLCLSLGYS
GDMTQAQRTSDSAGKQTRFQYRVPREGTTVWMDTMAIPVDAKHPEYAYEFINFVMRPENMAAISNFTGYPTSNAKARPNV
DEAMRNNPDIYLDEATYARLIPGKDIPQADMRARMRTWTKFKTATAK

Sequences:

>Translated_367_residues
MPTSHKWLLGALGLALAGLMPAVHAEDKTLRLYNWADYFAEDTLSKFTAETGIKVIYDVMDGSETLEAKMLSGGSGYDLI
FPGDTVAERLMRAGSLLPLDPSKITAMNDIEPGLQKLRSHYEHSNKATVPYTWGTIGLTYNAEQIQQRMADAPVNSLDML
FKPELAAKFADCGISMIDSPDEVLAVVLNYLGRDPRSAKPADLAAASDLLMKLRPYIRKFQSQPVTDLVNGNLCLSLGYS
GDMTQAQRTSDSAGKQTRFQYRVPREGTTVWMDTMAIPVDAKHPEYAYEFINFVMRPENMAAISNFTGYPTSNAKARPNV
DEAMRNNPDIYLDEATYARLIPGKDIPQADMRARMRTWTKFKTATAK
>Mature_366_residues
PTSHKWLLGALGLALAGLMPAVHAEDKTLRLYNWADYFAEDTLSKFTAETGIKVIYDVMDGSETLEAKMLSGGSGYDLIF
PGDTVAERLMRAGSLLPLDPSKITAMNDIEPGLQKLRSHYEHSNKATVPYTWGTIGLTYNAEQIQQRMADAPVNSLDMLF
KPELAAKFADCGISMIDSPDEVLAVVLNYLGRDPRSAKPADLAAASDLLMKLRPYIRKFQSQPVTDLVNGNLCLSLGYSG
DMTQAQRTSDSAGKQTRFQYRVPREGTTVWMDTMAIPVDAKHPEYAYEFINFVMRPENMAAISNFTGYPTSNAKARPNVD
EAMRNNPDIYLDEATYARLIPGKDIPQADMRARMRTWTKFKTATAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=366, Percent_Identity=41.8032786885246, Blast_Score=304, Evalue=5e-84,
Organism=Escherichia coli, GI1787367, Length=320, Percent_Identity=31.25, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40761; Mature: 40630

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSHKWLLGALGLALAGLMPAVHAEDKTLRLYNWADYFAEDTLSKFTAETGIKVIYDVM
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEHH
DGSETLEAKMLSGGSGYDLIFPGDTVAERLMRAGSLLPLDPSKITAMNDIEPGLQKLRSH
CCCHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHH
YEHSNKATVPYTWGTIGLTYNAEQIQQRMADAPVNSLDMLFKPELAAKFADCGISMIDSP
HHCCCCCCCCEECCCEEEEECHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHCCCCCCCCH
DEVLAVVLNYLGRDPRSAKPADLAAASDLLMKLRPYIRKFQSQPVTDLVNGNLCLSLGYS
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCC
GDMTQAQRTSDSAGKQTRFQYRVPREGTTVWMDTMAIPVDAKHPEYAYEFINFVMRPENM
CCCCHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCHH
AAISNFTGYPTSNAKARPNVDEAMRNNPDIYLDEATYARLIPGKDIPQADMRARMRTWTK
HHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCHHEEECCCCCCCHHHHHHHHHHHHH
FKTATAK
HHHCCCC
>Mature Secondary Structure 
PTSHKWLLGALGLALAGLMPAVHAEDKTLRLYNWADYFAEDTLSKFTAETGIKVIYDVM
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEEHH
DGSETLEAKMLSGGSGYDLIFPGDTVAERLMRAGSLLPLDPSKITAMNDIEPGLQKLRSH
CCCHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHHHHHHHH
YEHSNKATVPYTWGTIGLTYNAEQIQQRMADAPVNSLDMLFKPELAAKFADCGISMIDSP
HHCCCCCCCCEECCCEEEEECHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHCCCCCCCCH
DEVLAVVLNYLGRDPRSAKPADLAAASDLLMKLRPYIRKFQSQPVTDLVNGNLCLSLGYS
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCC
GDMTQAQRTSDSAGKQTRFQYRVPREGTTVWMDTMAIPVDAKHPEYAYEFINFVMRPENM
CCCCHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCHH
AAISNFTGYPTSNAKARPNVDEAMRNNPDIYLDEATYARLIPGKDIPQADMRARMRTWTK
HHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCHHEEECCCCCCCHHHHHHHHHHHHH
FKTATAK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]