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The map label for this gene is yafV [C]

Identifier: 77458598

GI number: 77458598

Start: 2729728

End: 2730636

Strand: Direct

Name: yafV [C]

Synonym: Pfl01_2371

Alternate gene names: 77458598

Gene position: 2729728-2730636 (Clockwise)

Preceding gene: 77458597

Following gene: 77458599

Centisome position: 42.4

GC content: 63.04

Gene sequence:

>909_bases
ATGACGATATTGAAAGTCGCCACCACCCAGATGCCGTGCACCTGGGACCTGAAAAGCAACCTTGATCGCGCCGAGCAGTT
GGTGCGTGAAGCGGCTGGGCAGGGCGCGCAAGTGATCCTGTTGCAGGAGCTGTTCGCCACGCCGTATTTCTGCATCGAGC
AGAGCCATCAGCACATGGCGCTGGCCGAGGCGTACCGCGACAGTCAGGTGCTGTCGCGATTCGCCGCGCTGGCCCGCGAA
CTGGGCGTGGTGTTACCGCTGAGCTGGTACGAGAAGGCCGGCAACGCCTACTTCAACTCGCTGAGCGTGGCGGATGCCGA
CGGGCGCCTGTTGGGCGTGTATCGCAAGACTCACATCCCCAACGCCATCGGTTATCAGGAGAAGGAGTATTTCAGCCCCG
GCGACACCGGTTTCAAAGTCTGGGACACCGCGTTCGGCCGCCTCGGCGTGGGCATCTGCTGGGATCAGTGGTTCCCGGAA
ACTGCCCGCTGCCTGGCGTTGATGGGGGCTGAGGTGCTGCTGTTCCCGACCGCCATCGGCTCGGAACCGGGCTGCGCGGA
TCTGGATTCGCGCGACCACTGGCAGATGACCATGCGCGGCCACGCGGCTGCCAATCTGCTGCCGGTGATCGCCTCCAACC
GGGTTGGCCGCGAAGTGGCCGGCACCGATGCGGCGTTGCAAATGAATTTCTACGGCTCATCGTTCATCTGCAACCACAAG
GGAAAAGTGCTCGCCGAAGCCGACCGCGCCAGCACCGGTGTCTTGGTGCAAAGCCTGGATCTGGCAGCCATGCGTGAAGA
TCGCCTGAGCTGGGGCATCTACCGCGACCGTCGTCCGGAAATGTATGGCGCTTTGCTCAGCCAGGACGGCCGTCACCTTA
ACGTCCGCTGGAATCCCCAAGGAGTCTGA

Upstream 100 bases:

>100_bases
TGAAGTGGTGCCGGTGCAGATCAATCACCTGGCCCATGGCGGCGGAGGTGTGCATTGCATCACCCAGCAACAGCCAGCCT
GGCCGGTGGAGGGTTGATCG

Downstream 100 bases:

>100_bases
TATGCCTACTTCACACAAGTGGTTGCTGGGCGCTCTGGGCCTGGCGCTGGCCGGCCTGATGCCGGCCGTGCACGCCGAAG
ACAAGACCTTACGGCTGTAC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTILKVATTQMPCTWDLKSNLDRAEQLVREAAGQGAQVILLQELFATPYFCIEQSHQHMALAEAYRDSQVLSRFAALARE
LGVVLPLSWYEKAGNAYFNSLSVADADGRLLGVYRKTHIPNAIGYQEKEYFSPGDTGFKVWDTAFGRLGVGICWDQWFPE
TARCLALMGAEVLLFPTAIGSEPGCADLDSRDHWQMTMRGHAAANLLPVIASNRVGREVAGTDAALQMNFYGSSFICNHK
GKVLAEADRASTGVLVQSLDLAAMREDRLSWGIYRDRRPEMYGALLSQDGRHLNVRWNPQGV

Sequences:

>Translated_302_residues
MTILKVATTQMPCTWDLKSNLDRAEQLVREAAGQGAQVILLQELFATPYFCIEQSHQHMALAEAYRDSQVLSRFAALARE
LGVVLPLSWYEKAGNAYFNSLSVADADGRLLGVYRKTHIPNAIGYQEKEYFSPGDTGFKVWDTAFGRLGVGICWDQWFPE
TARCLALMGAEVLLFPTAIGSEPGCADLDSRDHWQMTMRGHAAANLLPVIASNRVGREVAGTDAALQMNFYGSSFICNHK
GKVLAEADRASTGVLVQSLDLAAMREDRLSWGIYRDRRPEMYGALLSQDGRHLNVRWNPQGV
>Mature_301_residues
TILKVATTQMPCTWDLKSNLDRAEQLVREAAGQGAQVILLQELFATPYFCIEQSHQHMALAEAYRDSQVLSRFAALAREL
GVVLPLSWYEKAGNAYFNSLSVADADGRLLGVYRKTHIPNAIGYQEKEYFSPGDTGFKVWDTAFGRLGVGICWDQWFPET
ARCLALMGAEVLLFPTAIGSEPGCADLDSRDHWQMTMRGHAAANLLPVIASNRVGREVAGTDAALQMNFYGSSFICNHKG
KVLAEADRASTGVLVQSLDLAAMREDRLSWGIYRDRRPEMYGALLSQDGRHLNVRWNPQGV

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=294, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI297632348, Length=291, Percent_Identity=29.553264604811, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI297632350, Length=291, Percent_Identity=29.553264604811, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI5031947, Length=291, Percent_Identity=29.553264604811, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI7706509, Length=280, Percent_Identity=29.2857142857143, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI297632346, Length=201, Percent_Identity=31.3432835820896, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533173, Length=283, Percent_Identity=30.3886925795053, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17556280, Length=292, Percent_Identity=25.3424657534247, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323383, Length=293, Percent_Identity=26.2798634812287, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6322335, Length=308, Percent_Identity=26.2987012987013, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21358471, Length=303, Percent_Identity=28.7128712871287, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17933642, Length=283, Percent_Identity=25.4416961130742, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355835, Length=291, Percent_Identity=23.7113402061856, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 33506; Mature: 33375

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILKVATTQMPCTWDLKSNLDRAEQLVREAAGQGAQVILLQELFATPYFCIEQSHQHMA
CEEEEEECCCCCEEECHHHCHHHHHHHHHHHHCCCCEEEEEHHHHCCCEEEECCCCCHHH
LAEAYRDSQVLSRFAALARELGVVLPLSWYEKAGNAYFNSLSVADADGRLLGVYRKTHIP
HHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHCCCHHHCCEEEECCCCCEEEEEEHHCCC
NAIGYQEKEYFSPGDTGFKVWDTAFGRLGVGICWDQWFPETARCLALMGAEVLLFPTAIG
CCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCEEHHHCCCHHHHHHHHHCCCEEEEEECCC
SEPGCADLDSRDHWQMTMRGHAAANLLPVIASNRVGREVAGTDAALQMNFYGSSFICNHK
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHCCCCEEEEEEECCCEEEECCC
GKVLAEADRASTGVLVQSLDLAAMREDRLSWGIYRDRRPEMYGALLSQDGRHLNVRWNPQ
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEECCC
GV
CC
>Mature Secondary Structure 
TILKVATTQMPCTWDLKSNLDRAEQLVREAAGQGAQVILLQELFATPYFCIEQSHQHMA
EEEEEECCCCCEEECHHHCHHHHHHHHHHHHCCCCEEEEEHHHHCCCEEEECCCCCHHH
LAEAYRDSQVLSRFAALARELGVVLPLSWYEKAGNAYFNSLSVADADGRLLGVYRKTHIP
HHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHCCCHHHCCEEEECCCCCEEEEEEHHCCC
NAIGYQEKEYFSPGDTGFKVWDTAFGRLGVGICWDQWFPETARCLALMGAEVLLFPTAIG
CCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCEEHHHCCCHHHHHHHHHCCCEEEEEECCC
SEPGCADLDSRDHWQMTMRGHAAANLLPVIASNRVGREVAGTDAALQMNFYGSSFICNHK
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHCCCCEEEEEEECCCEEEECCC
GKVLAEADRASTGVLVQSLDLAAMREDRLSWGIYRDRRPEMYGALLSQDGRHLNVRWNPQ
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEECCC
GV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]