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The map label for this gene is cynX [H]

Identifier: 77458485

GI number: 77458485

Start: 2591012

End: 2592226

Strand: Reverse

Name: cynX [H]

Synonym: Pfl01_2258

Alternate gene names: 77458485

Gene position: 2592226-2591012 (Counterclockwise)

Preceding gene: 77458489

Following gene: 77458480

Centisome position: 40.26

GC content: 64.94

Gene sequence:

>1215_bases
ATGGCAATCACCCGCAATCTCTCCGGCCACCAACAGAGCGTCACGGCGGTGATCGGCCTGCTGATCCTGTCCATCGCCCT
GCGCCCGCCGATCATTTCGGTGGGGCCGATTTTGCTGTTGATCCAGCAGCACTTCCAGTTGAGCTATACCCAGGCCGCGC
TGCTGACCTCGATTCCCGACGTGTGCATGGGTGTGTTCGCGCTGGTGGTTCCGAGCCTGGCCAGGCGCTTCGGGATCGAT
CGCAGTGTGGTGGTCGCCCTGACGTTGCTGGGGGCCTCGACCTTGTTGCGGGCGATCTCGCCGAATGCATTCTTCCTGCT
CGGCAGTACGTTCTTCGCCAGCATCGGCATTGCGGTTGCCGGCGCGATGACCGGCGCCTGGATCAAGACCCACTTTGCTC
AGCGACCAGCGCTGTTCATGGGCATCTACGCCGGCGGCTTGAGTCTGGGCGCGACTCTGGCGGCAGTGCTCAGCGCGCCG
ATTGCCGAGCTGGCGCAGGACTGGCGGGTCAGCGCCGGGTTCTGGAGCGTGCTGTGCCTCACCGCCATCGCCAGTTGGGT
GTGGATGGCACGCCGTTTTGCGACACCGGCGCCTGTAGTGAAATCGTCGTCGAGTCCGAGCAAACGCCTGCCCTGGAGCA
ATCCGCAGGCATGGCTGATCGCGTTGAATTTCGGGGCCGGGCAATTCGTGGTCTATGCCCTCTTCGCCTGGATGGCGCCC
GCCTCGGTGGAAGCGGCCACGTCGAGTGTTTCCCCGGGCGTCCTGCTGGGCATTTTTACCGCGGTGTTCGCCGTGGCCAG
CGTGGGTGCGGGGCTGATTCCGGGCAAGGCCCACGACCGGCGCGGTCTGCTCGGGTTGTCGGCGTTGCTGGCCCTGCTCG
GGGTGGGCGGCATGGCGTTTCTGCCGGCTTACTGGCCGATGCTCTACGTGGTACTTGCGGCCATCGGTCTGGGCATGGGC
TTCACCGTGGCCATGACCTTGCCGCTGGATCACGCCAGCACCAGCGAACAGGCCGGGTTGTGGACGGTGTTCATGTTGTT
CATCGGTTACCTGATCGCCGCGCTCGGGCCGCTGCTGTTCGGCGCCTTGCGCGAATACGCCGGACACTATGGCCCGGCGT
ACACCTTGCTGTTCGCGGTACTGCTGTTGATGCTCGGCATCACCCCTCTGCTCAGACTGGCACCGGCAACATCTCCGCAG
GTGTCCGTTGCCTGA

Upstream 100 bases:

>100_bases
GTTAAAGCGGCAATTGTCACTTTGGCGACAAACCCTGGCATTTGCCATGGCCGGCCCATTGTTAGCCTTGCGCTCATGGT
TCATCAATGAGGCTTTTCCC

Downstream 100 bases:

>100_bases
GCGGGGGATGACAACCCTGCGACGCGCCGTAGCACGTCGCAGGGTTTCAGTTGGCCGCAGCGGCTTTTTCAATCAGCCGC
GCCACGGGTGCCGGGTTCGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; Cyanate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MAITRNLSGHQQSVTAVIGLLILSIALRPPIISVGPILLLIQQHFQLSYTQAALLTSIPDVCMGVFALVVPSLARRFGID
RSVVVALTLLGASTLLRAISPNAFFLLGSTFFASIGIAVAGAMTGAWIKTHFAQRPALFMGIYAGGLSLGATLAAVLSAP
IAELAQDWRVSAGFWSVLCLTAIASWVWMARRFATPAPVVKSSSSPSKRLPWSNPQAWLIALNFGAGQFVVYALFAWMAP
ASVEAATSSVSPGVLLGIFTAVFAVASVGAGLIPGKAHDRRGLLGLSALLALLGVGGMAFLPAYWPMLYVVLAAIGLGMG
FTVAMTLPLDHASTSEQAGLWTVFMLFIGYLIAALGPLLFGALREYAGHYGPAYTLLFAVLLLMLGITPLLRLAPATSPQ
VSVA

Sequences:

>Translated_404_residues
MAITRNLSGHQQSVTAVIGLLILSIALRPPIISVGPILLLIQQHFQLSYTQAALLTSIPDVCMGVFALVVPSLARRFGID
RSVVVALTLLGASTLLRAISPNAFFLLGSTFFASIGIAVAGAMTGAWIKTHFAQRPALFMGIYAGGLSLGATLAAVLSAP
IAELAQDWRVSAGFWSVLCLTAIASWVWMARRFATPAPVVKSSSSPSKRLPWSNPQAWLIALNFGAGQFVVYALFAWMAP
ASVEAATSSVSPGVLLGIFTAVFAVASVGAGLIPGKAHDRRGLLGLSALLALLGVGGMAFLPAYWPMLYVVLAAIGLGMG
FTVAMTLPLDHASTSEQAGLWTVFMLFIGYLIAALGPLLFGALREYAGHYGPAYTLLFAVLLLMLGITPLLRLAPATSPQ
VSVA
>Mature_403_residues
AITRNLSGHQQSVTAVIGLLILSIALRPPIISVGPILLLIQQHFQLSYTQAALLTSIPDVCMGVFALVVPSLARRFGIDR
SVVVALTLLGASTLLRAISPNAFFLLGSTFFASIGIAVAGAMTGAWIKTHFAQRPALFMGIYAGGLSLGATLAAVLSAPI
AELAQDWRVSAGFWSVLCLTAIASWVWMARRFATPAPVVKSSSSPSKRLPWSNPQAWLIALNFGAGQFVVYALFAWMAPA
SVEAATSSVSPGVLLGIFTAVFAVASVGAGLIPGKAHDRRGLLGLSALLALLGVGGMAFLPAYWPMLYVVLAAIGLGMGF
TVAMTLPLDHASTSEQAGLWTVFMLFIGYLIAALGPLLFGALREYAGHYGPAYTLLFAVLLLMLGITPLLRLAPATSPQV
SVA

Specific function: This protein is part of an active transport system that transports exogenous cyanate into E.coli cells [H]

COG id: COG2807

COG function: function code P; Cyanate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Cyanate porter (TC 2.A.1.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786536, Length=386, Percent_Identity=31.8652849740933, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788092, Length=375, Percent_Identity=26.1333333333333, Blast_Score=111, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004747
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42278; Mature: 42147

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITRNLSGHQQSVTAVIGLLILSIALRPPIISVGPILLLIQQHFQLSYTQAALLTSIPD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCMGVFALVVPSLARRFGIDRSVVVALTLLGASTLLRAISPNAFFLLGSTFFASIGIAVA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
GAMTGAWIKTHFAQRPALFMGIYAGGLSLGATLAAVLSAPIAELAQDWRVSAGFWSVLCL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TAIASWVWMARRFATPAPVVKSSSSPSKRLPWSNPQAWLIALNFGAGQFVVYALFAWMAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCC
ASVEAATSSVSPGVLLGIFTAVFAVASVGAGLIPGKAHDRRGLLGLSALLALLGVGGMAF
CHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
LPAYWPMLYVVLAAIGLGMGFTVAMTLPLDHASTSEQAGLWTVFMLFIGYLIAALGPLLF
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GALREYAGHYGPAYTLLFAVLLLMLGITPLLRLAPATSPQVSVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AITRNLSGHQQSVTAVIGLLILSIALRPPIISVGPILLLIQQHFQLSYTQAALLTSIPD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VCMGVFALVVPSLARRFGIDRSVVVALTLLGASTLLRAISPNAFFLLGSTFFASIGIAVA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
GAMTGAWIKTHFAQRPALFMGIYAGGLSLGATLAAVLSAPIAELAQDWRVSAGFWSVLCL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TAIASWVWMARRFATPAPVVKSSSSPSKRLPWSNPQAWLIALNFGAGQFVVYALFAWMAP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCC
ASVEAATSSVSPGVLLGIFTAVFAVASVGAGLIPGKAHDRRGLLGLSALLALLGVGGMAF
CHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHH
LPAYWPMLYVVLAAIGLGMGFTVAMTLPLDHASTSEQAGLWTVFMLFIGYLIAALGPLLF
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GALREYAGHYGPAYTLLFAVLLLMLGITPLLRLAPATSPQVSVA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; Cyanate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + Cyanate [Periplasm] = Proton [Cytoplasm] + Cyanate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3049588; 9278503 [H]