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The map label for this gene is rhtB [H]

Identifier: 77458387

GI number: 77458387

Start: 2442242

End: 2442862

Strand: Reverse

Name: rhtB [H]

Synonym: Pfl01_2160

Alternate gene names: 77458387

Gene position: 2442862-2442242 (Counterclockwise)

Preceding gene: 77458390

Following gene: 77458385

Centisome position: 37.94

GC content: 64.57

Gene sequence:

>621_bases
ATGGATATCGCCACCCTCTCCCTGTTTCTGCCCGCCTGTTTCGCCCTGAACATGGCACCGGGGCCGAACAATCTGCTGTC
GGTCAGCAACGCCACCCGCTACGGCTATCGTCGCGCCTGCGTGGCCGGTGTCGGTCGGTTGCTGGCGTTCGCCGGGATGA
TCGCCCTCGCGGCGGCGGGGCTGGCGGTGGTACTGCAAACCTCTGAACTGTTGTTCTATGGGATCAAGATCATCGGCGCG
GGGTATCTGCTGTACCTGGCGTGGCAGCTGTGGCGGGCCAATCCTGCCGCCGAAGACGCCGTTGCGGCGTCGGCGAACGG
ACTTATGGCTCTGGCTCGCCAGGAATTCCTGGTGGCAGCGGGAAATCCCAAGGCAATCCTTATCTTCACCGCCTTCCTGC
CGCAATTCGTCGACCCGGCCCGCGCCATCACCCCGCAATTCATGTTGCTGGGCGCGCTGTTTCTGTTGCTGGAATGGATC
GCCATCAGCGCCTACGCGTACATGGGGCTGCACATGCGCCGCTGGTTCGCCGAGCCGCGCGGCAAGCGGATTTTCAACCG
TTGCTGCGCCGGGCTGCTGTCGGCGGCGGCTTCGGTGTTGCTGATGGCCAAGCGGGCCTAG

Upstream 100 bases:

>100_bases
TGCCGATAGTCGCTGTACCGCGACTCTGGCGCCTGACTGTACGGTTAGGCCCGCACACCCGCGTGATAAAAATAACGCTC
CCGTCACCGGAGCCCGAGTC

Downstream 100 bases:

>100_bases
GCTTCGGACGGGCCTTTGAGTTCGACCTGATTGCCGTCCGGATCAAAGCAGTACAACGACAGGCCGTTGCCCTCGGCGCC
GAAACGGGTGGCGGCCTTGT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MDIATLSLFLPACFALNMAPGPNNLLSVSNATRYGYRRACVAGVGRLLAFAGMIALAAAGLAVVLQTSELLFYGIKIIGA
GYLLYLAWQLWRANPAAEDAVAASANGLMALARQEFLVAAGNPKAILIFTAFLPQFVDPARAITPQFMLLGALFLLLEWI
AISAYAYMGLHMRRWFAEPRGKRIFNRCCAGLLSAAASVLLMAKRA

Sequences:

>Translated_206_residues
MDIATLSLFLPACFALNMAPGPNNLLSVSNATRYGYRRACVAGVGRLLAFAGMIALAAAGLAVVLQTSELLFYGIKIIGA
GYLLYLAWQLWRANPAAEDAVAASANGLMALARQEFLVAAGNPKAILIFTAFLPQFVDPARAITPQFMLLGALFLLLEWI
AISAYAYMGLHMRRWFAEPRGKRIFNRCCAGLLSAAASVLLMAKRA
>Mature_206_residues
MDIATLSLFLPACFALNMAPGPNNLLSVSNATRYGYRRACVAGVGRLLAFAGMIALAAAGLAVVLQTSELLFYGIKIIGA
GYLLYLAWQLWRANPAAEDAVAASANGLMALARQEFLVAAGNPKAILIFTAFLPQFVDPARAITPQFMLLGALFLLLEWI
AISAYAYMGLHMRRWFAEPRGKRIFNRCCAGLLSAAASVLLMAKRA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=198, Percent_Identity=26.7676767676768, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788099, Length=150, Percent_Identity=30.6666666666667, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22147; Mature: 22147

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIATLSLFLPACFALNMAPGPNNLLSVSNATRYGYRRACVAGVGRLLAFAGMIALAAAG
CCHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVVLQTSELLFYGIKIIGAGYLLYLAWQLWRANPAAEDAVAASANGLMALARQEFLVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHEEEC
GNPKAILIFTAFLPQFVDPARAITPQFMLLGALFLLLEWIAISAYAYMGLHMRRWFAEPR
CCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GKRIFNRCCAGLLSAAASVLLMAKRA
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIATLSLFLPACFALNMAPGPNNLLSVSNATRYGYRRACVAGVGRLLAFAGMIALAAAG
CCHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVVLQTSELLFYGIKIIGAGYLLYLAWQLWRANPAAEDAVAASANGLMALARQEFLVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHEEEC
GNPKAILIFTAFLPQFVDPARAITPQFMLLGALFLLLEWIAISAYAYMGLHMRRWFAEPR
CCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GKRIFNRCCAGLLSAAASVLLMAKRA
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]