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The map label for this gene is ada [H]

Identifier: 77458385

GI number: 77458385

Start: 2440640

End: 2441722

Strand: Reverse

Name: ada [H]

Synonym: Pfl01_2158

Alternate gene names: 77458385

Gene position: 2441722-2440640 (Counterclockwise)

Preceding gene: 77458387

Following gene: 77458384

Centisome position: 37.92

GC content: 62.88

Gene sequence:

>1083_bases
ATGAACATACAGACCGCTGTCCTGCCCCCTCACGCCGAAATGGTTCGCGCCATGCTCGCAAGCGACACTGCCTATGAAGG
CGTGTTTTTCACGGCCGTGAAAACCACCGGGATCTTTTGCCGCCCTACCTGCACGGCGCGCAAGCCGAAACCGGAAAACG
TCGAGTTCTTCGCCCACGCTGACGATTGCCTGTCCGCTGGTTACCGCGCCTGTTTGCGCTGCAAACCGCTGGATGCCGCG
GCCATCGCCCCGGATTGGGTGCAGCAGCTGCTAAAAGCGGTCGACGCCGATCCGGAACTTCGCTGGAGCGATGCGCAATT
GCTCGCCGAAGGTATTGAACCGCTGAAGCTGCGGCGCTGGTTCAAGCAGCATTTCGGCATGACCTTCCATGCGTGGCTGC
GCACCCGCCGACTCGGCGTGGCGCTGGGCGGGATCAAGCAAGGCACGTCCATCGACCATGCCGCGTTCGACTCCGGTTAT
GAATCCCTCAGTGGTTTTCGCGATGCGTTTCAGAAGTCGTTCCACATCACGCCGGGGCGCGCAGCGGCCAGTGAACCGCT
GCTGTTCACCCGCCTGACCACGCCATTGGGTCCTATGATTGCCATGGCCGAGCGGCGCGGGCTGGTCTTGCTGGAGTTTC
TCGATCGCCCGGCGCTGACCCGCGAAGTCGAGGAATTGCAGAATCGCTACGGCTACGTGGTCGCACCGGGGCAGAACGCG
CATTTGCAGCAGATCGACAACGAGCTGGCGCTGTACTTCGCCGGCAAGCTGAGCGAATTCAGTGTGCCACTGCACCTGCC
CGGCAGCGAGTTTGCCCAGCAGGTCTGGGCCGAACTTCGGCAAATCCCTTACGGCCAAACCAGCACCTACGGCACCATCG
CCGCACGCCTGGGCAAACCCGGCGCCAGCCGAGCAGTGGGGCTGGCCAACGGACACAATCGGCTGTCGATCGTGGTGCCA
TGCCATCGGGTGATCGGTGCGGACGGCTCGTTGACCGGATATGGTGGTGGGCAACCGCGCAAGGCGTTTTTGCTCCGGCT
GGAGAACGCCGCCGTGCAGCTCACGCAACAACTGGCTTTCTGA

Upstream 100 bases:

>100_bases
TCCCGAGTTGTAGCGACCCGATCACTGTAGTGCAGCGGCCCGAAAATTCACTGTCGATTTTTCGGTGGAGCCATCGAATA
ACTGGTTTTATCCTCGGCCC

Downstream 100 bases:

>100_bases
CCAACGACAAGAAGGGATTTCGATGAACGCGCTCGACACTCAGTTTCACCAGTTGCACCACGATGACCTGCTGATCCTGA
CCAACGTTGCCGACGCCACC

Product: transcriptional regulator Ada / DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MNIQTAVLPPHAEMVRAMLASDTAYEGVFFTAVKTTGIFCRPTCTARKPKPENVEFFAHADDCLSAGYRACLRCKPLDAA
AIAPDWVQQLLKAVDADPELRWSDAQLLAEGIEPLKLRRWFKQHFGMTFHAWLRTRRLGVALGGIKQGTSIDHAAFDSGY
ESLSGFRDAFQKSFHITPGRAAASEPLLFTRLTTPLGPMIAMAERRGLVLLEFLDRPALTREVEELQNRYGYVVAPGQNA
HLQQIDNELALYFAGKLSEFSVPLHLPGSEFAQQVWAELRQIPYGQTSTYGTIAARLGKPGASRAVGLANGHNRLSIVVP
CHRVIGADGSLTGYGGGQPRKAFLLRLENAAVQLTQQLAF

Sequences:

>Translated_360_residues
MNIQTAVLPPHAEMVRAMLASDTAYEGVFFTAVKTTGIFCRPTCTARKPKPENVEFFAHADDCLSAGYRACLRCKPLDAA
AIAPDWVQQLLKAVDADPELRWSDAQLLAEGIEPLKLRRWFKQHFGMTFHAWLRTRRLGVALGGIKQGTSIDHAAFDSGY
ESLSGFRDAFQKSFHITPGRAAASEPLLFTRLTTPLGPMIAMAERRGLVLLEFLDRPALTREVEELQNRYGYVVAPGQNA
HLQQIDNELALYFAGKLSEFSVPLHLPGSEFAQQVWAELRQIPYGQTSTYGTIAARLGKPGASRAVGLANGHNRLSIVVP
CHRVIGADGSLTGYGGGQPRKAFLLRLENAAVQLTQQLAF
>Mature_360_residues
MNIQTAVLPPHAEMVRAMLASDTAYEGVFFTAVKTTGIFCRPTCTARKPKPENVEFFAHADDCLSAGYRACLRCKPLDAA
AIAPDWVQQLLKAVDADPELRWSDAQLLAEGIEPLKLRRWFKQHFGMTFHAWLRTRRLGVALGGIKQGTSIDHAAFDSGY
ESLSGFRDAFQKSFHITPGRAAASEPLLFTRLTTPLGPMIAMAERRGLVLLEFLDRPALTREVEELQNRYGYVVAPGQNA
HLQQIDNELALYFAGKLSEFSVPLHLPGSEFAQQVWAELRQIPYGQTSTYGTIAARLGKPGASRAVGLANGHNRLSIVVP
CHRVIGADGSLTGYGGGQPRKAFLLRLENAAVQLTQQLAF

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=167, Percent_Identity=32.3353293413174, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788542, Length=345, Percent_Identity=33.9130434782609, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787596, Length=167, Percent_Identity=41.3173652694611, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115533068, Length=92, Percent_Identity=41.304347826087, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=92, Percent_Identity=41.304347826087, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=46.9879518072289, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39546; Mature: 39546

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQTAVLPPHAEMVRAMLASDTAYEGVFFTAVKTTGIFCRPTCTARKPKPENVEFFAHA
CCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCEEEEEEC
DDCLSAGYRACLRCKPLDAAAIAPDWVQQLLKAVDADPELRWSDAQLLAEGIEPLKLRRW
HHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHH
FKQHFGMTFHAWLRTRRLGVALGGIKQGTSIDHAAFDSGYESLSGFRDAFQKSFHITPGR
HHHHCCCCHHHHHHHHHHHHEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCC
AAASEPLLFTRLTTPLGPMIAMAERRGLVLLEFLDRPALTREVEELQNRYGYVVAPGQNA
CCCCCCEEEEECCCCHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHHHCCEEEECCCCC
HLQQIDNELALYFAGKLSEFSVPLHLPGSEFAQQVWAELRQIPYGQTSTYGTIAARLGKP
HHHHHCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
GASRAVGLANGHNRLSIVVPCHRVIGADGSLTGYGGGQPRKAFLLRLENAAVQLTQQLAF
CCCCEEEECCCCCEEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIQTAVLPPHAEMVRAMLASDTAYEGVFFTAVKTTGIFCRPTCTARKPKPENVEFFAHA
CCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCEEECCCCCCCCCCCCCEEEEEEC
DDCLSAGYRACLRCKPLDAAAIAPDWVQQLLKAVDADPELRWSDAQLLAEGIEPLKLRRW
HHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHH
FKQHFGMTFHAWLRTRRLGVALGGIKQGTSIDHAAFDSGYESLSGFRDAFQKSFHITPGR
HHHHCCCCHHHHHHHHHHHHEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCC
AAASEPLLFTRLTTPLGPMIAMAERRGLVLLEFLDRPALTREVEELQNRYGYVVAPGQNA
CCCCCCEEEEECCCCHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHHHCCEEEECCCCC
HLQQIDNELALYFAGKLSEFSVPLHLPGSEFAQQVWAELRQIPYGQTSTYGTIAARLGKP
HHHHHCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
GASRAVGLANGHNRLSIVVPCHRVIGADGSLTGYGGGQPRKAFLLRLENAAVQLTQQLAF
CCCCEEEECCCCCEEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]