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The map label for this gene is braD [H]

Identifier: 77457474

GI number: 77457474

Start: 1417243

End: 1418166

Strand: Reverse

Name: braD [H]

Synonym: Pfl01_1247

Alternate gene names: 77457474

Gene position: 1418166-1417243 (Counterclockwise)

Preceding gene: 77457475

Following gene: 77457473

Centisome position: 22.03

GC content: 60.28

Gene sequence:

>924_bases
ATGCCTGACATCTATCACTTCTTCCAACAGCTGGTTAACGGCTTGAACGTTGGCAGCATGTATGCCCTGATCGCCATCGG
CTACACGATGGTCTACGGCATCATTGGAATGATCAACTTCGCCCACGGCGAGGTGTACATGATCGGTTCCTACGTGGCGT
TCATCGCCATCGCCGGGCTGACCATGATGGGACTCGAAAGCGTCCCGCTGTTGATGACCGTGGCGTTCATCGCCAGCATC
GTCGTCACCAGCTCCTACGGATACAGCATCGAACGCATTGCCTACCGCCCGTTGCGCGGCAGCAACCGTCTGATCCCGCT
GATTTCCGCGATCGGTATGTCGATCTTCCTGCAGAACACGGTTCTGCTGGCGCAAGACTCCAAGGACAAAGCAATCCCCA
ACCTGATTCCGGGCAACTTCGCCTTCGGCCCAGGTGGCGCACATGAAGTGCTGATTTCCTACATGCAAATCGTGGTGTTC
GTGGTGACCCTGATCGCCATGCTCGGCCTCACGCTGTTCATCTCCCGCTCTCGCCTGGGCCGCGCCTGCCGCGCCTGCGC
CGAAGACATCAAGATGGCCAACCTGCTGGGCATCAACACCAACAACATCATCGCCCTGACCTTCGTCATCGGTGCTGCGC
TGGCGGCCGTCGCGGCCGTGCTGCTGAGCATGCAATACGGCGTGATCAACCCGAACGCCGGTTTCCTCGTCGGCCTCAAG
GCCTTCACCGCCGCAGTGCTGGGCGGGATCGGCAGCATCCCCGGCGCCATGCTCGGCGGGCTGGTGCTCGGGGTGGCGGA
AGCCTTTGGCGCCGATATCTTCGGCGACCAATACAAGGACGTCGTGGCTTTCGGCTTGTTGGTTCTGGTGCTGTTGTTCC
GTCCGACCGGCATTCTGGGCCGTCCGGAGGTTGAGAAAGTATGA

Upstream 100 bases:

>100_bases
GCTGGCAAAACCCGGATTCGAAGTGGATATAGATCCACGGGGCCGGGCGGGAAAATGACTCCACCAGTGAAATGCGTATC
AGGTTTTTAGGAGCGCTGTA

Downstream 100 bases:

>100_bases
CTAGGAATCTTAAACAGGCGTTGTTCAGCGCCTTGCTGGTCTGGGCCGTGGCCTATCCGGTACTGGGCCTGAAACTGACT
ATCGTCGGCACCAATCTGGA

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPDIYHFFQQLVNGLNVGSMYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIAGLTMMGLESVPLLMTVAFIASI
VVTSSYGYSIERIAYRPLRGSNRLIPLISAIGMSIFLQNTVLLAQDSKDKAIPNLIPGNFAFGPGGAHEVLISYMQIVVF
VVTLIAMLGLTLFISRSRLGRACRACAEDIKMANLLGINTNNIIALTFVIGAALAAVAAVLLSMQYGVINPNAGFLVGLK
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFGLLVLVLLFRPTGILGRPEVEKV

Sequences:

>Translated_307_residues
MPDIYHFFQQLVNGLNVGSMYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIAGLTMMGLESVPLLMTVAFIASI
VVTSSYGYSIERIAYRPLRGSNRLIPLISAIGMSIFLQNTVLLAQDSKDKAIPNLIPGNFAFGPGGAHEVLISYMQIVVF
VVTLIAMLGLTLFISRSRLGRACRACAEDIKMANLLGINTNNIIALTFVIGAALAAVAAVLLSMQYGVINPNAGFLVGLK
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFGLLVLVLLFRPTGILGRPEVEKV
>Mature_306_residues
PDIYHFFQQLVNGLNVGSMYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIAGLTMMGLESVPLLMTVAFIASIV
VTSSYGYSIERIAYRPLRGSNRLIPLISAIGMSIFLQNTVLLAQDSKDKAIPNLIPGNFAFGPGGAHEVLISYMQIVVFV
VTLIAMLGLTLFISRSRLGRACRACAEDIKMANLLGINTNNIIALTFVIGAALAAVAAVLLSMQYGVINPNAGFLVGLKA
FTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFGLLVLVLLFRPTGILGRPEVEKV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=302, Percent_Identity=66.2251655629139, Blast_Score=385, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 32450; Mature: 32319

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIYHFFQQLVNGLNVGSMYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIAGL
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
TMMGLESVPLLMTVAFIASIVVTSSYGYSIERIAYRPLRGSNRLIPLISAIGMSIFLQNT
HHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHCE
VLLAQDSKDKAIPNLIPGNFAFGPGGAHEVLISYMQIVVFVVTLIAMLGLTLFISRSRLG
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RACRACAEDIKMANLLGINTNNIIALTFVIGAALAAVAAVLLSMQYGVINPNAGFLVGLK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFGLLVLVLLFRPTGILG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
RPEVEKV
CCCCCCC
>Mature Secondary Structure 
PDIYHFFQQLVNGLNVGSMYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVAFIAIAGL
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
TMMGLESVPLLMTVAFIASIVVTSSYGYSIERIAYRPLRGSNRLIPLISAIGMSIFLQNT
HHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHCE
VLLAQDSKDKAIPNLIPGNFAFGPGGAHEVLISYMQIVVFVVTLIAMLGLTLFISRSRLG
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RACRACAEDIKMANLLGINTNNIIALTFVIGAALAAVAAVLLSMQYGVINPNAGFLVGLK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
AFTAAVLGGIGSIPGAMLGGLVLGVAEAFGADIFGDQYKDVVAFGLLVLVLLFRPTGILG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
RPEVEKV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]