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The map label for this gene is braC [H]

Identifier: 77457475

GI number: 77457475

Start: 1418398

End: 1419525

Strand: Reverse

Name: braC [H]

Synonym: Pfl01_1248

Alternate gene names: 77457475

Gene position: 1419525-1418398 (Counterclockwise)

Preceding gene: 77457477

Following gene: 77457474

Centisome position: 22.05

GC content: 58.24

Gene sequence:

>1128_bases
ATGACTAAGGCTACTAAGCAGATTTCCAAACTGTTTGCCGCTATGGTTCTGGCCGGGGTTGCCAGCCATTCGTTCGCAGC
TGACACCATCAAGATCGGTATCGCCGGCCCTAAAACCGGTCCAGTAGCCCAGTACGGCGACATGCAGTTCAGTGGCGCGA
AAATGGCCATCGAACAGATCAACGCCAAAGGCGGCGTCGACGGCAAGAAGCTCGAAGCCGTTGAATACGATGACGCCTGC
GATCCAAAACAAGCGGTAGCAGTCGCGAACAAGGTCGTCAACGACGGCGTCAAGTTCGTGGTCGGCCACCTGTGCTCCAG
CTCCACCCAGCCCGCTTCGGACATCTACGAAGACGAAGGCGTGATAATGATCACCCCGGCTGCCACCAGCCCGGACATCA
CCAACCGTGGTTACAAAATGGTCTTCCGCACCATCGGTCTGGACAGCGCCCAAGGCCCTGCAGCCGGTAACTACATCGCC
GATCACGTGAAGCCGAAAATCGTCGGCGTGCTGCACGACAAGCAGCAGTACGGTGAAGGCATCGCCACCGCCGTCAAATC
CACCCTCGAGAAGAAAGGCACCAAGGTTGCCGTGTTCGAAGGCGTCAACGCCGGCGACAAGGACTTCTCCGCGATCATCG
CCAAGCTCAAGCAAGCCAACGTCGACTTCGTCTACTACGGCGGCTACCACCCGGAGCTGGGCCTGATCCTGCGTCAAGCC
CAGGAAAAAGGCCTGAAAGCCAAGTTCATGGGTCCGGAAGGCGTGGGTAACGACTCGATCACCCAGATCGCCAAGGACGC
TGCCGAAGGCCTGCTGGTGACCCTGCCGAAATCCTTCGACAAGGATCCGGCCAACGTGGCCCTGGCCGATGCATTCAAGG
CCAAGAAAGAAGACCCGAGCGGCCCGTTCGTGTTCCCGTCCTACTCGGCGGTTGAAGTGATTGCCGAAGGTATCAAGGCT
GCCAAGTCCGAAGATGCTGCCAAAGTAGCTGAAGCCATCCACAAAGGCACCTTCAAGACTCCGACCGGCGACCTGAGCTT
CGACGACAAGGGCGACCTGAAGGACTTCAAGTTCGTGGTTTACACATGGCACGCTAACGGCCCAAAAACTGAAGCGTCGC
CTCAGTAA

Upstream 100 bases:

>100_bases
GCCGGGGGCCTTTCCGGAGGGTATCGCCTAGATATCTGTCCCGGTTCTGCTGCTACGGCGTCAGGACTCTAAAAAAATAC
AACAGTAAGAGTGGAGCACT

Downstream 100 bases:

>100_bases
GGCGCTGCCTGACTGACTGCCAATAAAGCCCACGGCGTGCCGTGGGCTTTGTTTTACGAACGTATTGGGCCGCGCTGGCG
TGATCCGCCAGTCTCCCCAC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MTKATKQISKLFAAMVLAGVASHSFAADTIKIGIAGPKTGPVAQYGDMQFSGAKMAIEQINAKGGVDGKKLEAVEYDDAC
DPKQAVAVANKVVNDGVKFVVGHLCSSSTQPASDIYEDEGVIMITPAATSPDITNRGYKMVFRTIGLDSAQGPAAGNYIA
DHVKPKIVGVLHDKQQYGEGIATAVKSTLEKKGTKVAVFEGVNAGDKDFSAIIAKLKQANVDFVYYGGYHPELGLILRQA
QEKGLKAKFMGPEGVGNDSITQIAKDAAEGLLVTLPKSFDKDPANVALADAFKAKKEDPSGPFVFPSYSAVEVIAEGIKA
AKSEDAAKVAEAIHKGTFKTPTGDLSFDDKGDLKDFKFVVYTWHANGPKTEASPQ

Sequences:

>Translated_375_residues
MTKATKQISKLFAAMVLAGVASHSFAADTIKIGIAGPKTGPVAQYGDMQFSGAKMAIEQINAKGGVDGKKLEAVEYDDAC
DPKQAVAVANKVVNDGVKFVVGHLCSSSTQPASDIYEDEGVIMITPAATSPDITNRGYKMVFRTIGLDSAQGPAAGNYIA
DHVKPKIVGVLHDKQQYGEGIATAVKSTLEKKGTKVAVFEGVNAGDKDFSAIIAKLKQANVDFVYYGGYHPELGLILRQA
QEKGLKAKFMGPEGVGNDSITQIAKDAAEGLLVTLPKSFDKDPANVALADAFKAKKEDPSGPFVFPSYSAVEVIAEGIKA
AKSEDAAKVAEAIHKGTFKTPTGDLSFDDKGDLKDFKFVVYTWHANGPKTEASPQ
>Mature_374_residues
TKATKQISKLFAAMVLAGVASHSFAADTIKIGIAGPKTGPVAQYGDMQFSGAKMAIEQINAKGGVDGKKLEAVEYDDACD
PKQAVAVANKVVNDGVKFVVGHLCSSSTQPASDIYEDEGVIMITPAATSPDITNRGYKMVFRTIGLDSAQGPAAGNYIAD
HVKPKIVGVLHDKQQYGEGIATAVKSTLEKKGTKVAVFEGVNAGDKDFSAIIAKLKQANVDFVYYGGYHPELGLILRQAQ
EKGLKAKFMGPEGVGNDSITQIAKDAAEGLLVTLPKSFDKDPANVALADAFKAKKEDPSGPFVFPSYSAVEVIAEGIKAA
KSEDAAKVAEAIHKGTFKTPTGDLSFDDKGDLKDFKFVVYTWHANGPKTEASPQ

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=367, Percent_Identity=56.1307901907357, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI48994941, Length=361, Percent_Identity=57.617728531856, Blast_Score=446, Evalue=1e-126,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39579; Mature: 39448

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKATKQISKLFAAMVLAGVASHSFAADTIKIGIAGPKTGPVAQYGDMQFSGAKMAIEQI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEECCHHHHHHHH
NAKGGVDGKKLEAVEYDDACDPKQAVAVANKVVNDGVKFVVGHLCSSSTQPASDIYEDEG
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
VIMITPAATSPDITNRGYKMVFRTIGLDSAQGPAAGNYIADHVKPKIVGVLHDKQQYGEG
EEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECHHHHCCH
IATAVKSTLEKKGTKVAVFEGVNAGDKDFSAIIAKLKQANVDFVYYGGYHPELGLILRQA
HHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
QEKGLKAKFMGPEGVGNDSITQIAKDAAEGLLVTLPKSFDKDPANVALADAFKAKKEDPS
HHCCCEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCEEHHHHHHHCCCCCC
GPFVFPSYSAVEVIAEGIKAAKSEDAAKVAEAIHKGTFKTPTGDLSFDDKGDLKDFKFVV
CCEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
YTWHANGPKTEASPQ
EEEECCCCCCCCCCC
>Mature Secondary Structure 
TKATKQISKLFAAMVLAGVASHSFAADTIKIGIAGPKTGPVAQYGDMQFSGAKMAIEQI
CHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEECCHHHHHHHH
NAKGGVDGKKLEAVEYDDACDPKQAVAVANKVVNDGVKFVVGHLCSSSTQPASDIYEDEG
CCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
VIMITPAATSPDITNRGYKMVFRTIGLDSAQGPAAGNYIADHVKPKIVGVLHDKQQYGEG
EEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECHHHHCCH
IATAVKSTLEKKGTKVAVFEGVNAGDKDFSAIIAKLKQANVDFVYYGGYHPELGLILRQA
HHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
QEKGLKAKFMGPEGVGNDSITQIAKDAAEGLLVTLPKSFDKDPANVALADAFKAKKEDPS
HHCCCEEEECCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCEEHHHHHHHCCCCCC
GPFVFPSYSAVEVIAEGIKAAKSEDAAKVAEAIHKGTFKTPTGDLSFDDKGDLKDFKFVV
CCEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
YTWHANGPKTEASPQ
EEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]