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The map label for this gene is nemA [C]

Identifier: 77457111

GI number: 77457111

Start: 1031419

End: 1032522

Strand: Direct

Name: nemA [C]

Synonym: Pfl01_0884

Alternate gene names: 77457111

Gene position: 1031419-1032522 (Clockwise)

Preceding gene: 77457110

Following gene: 77457114

Centisome position: 16.02

GC content: 61.96

Gene sequence:

>1104_bases
ATGCCCGTGCAAGCCCTGTTCAAACCGTTCCAGCTCGGTGCCCTCGAACTGCCGACCCGCGTGGTCATGGCGCCGATGAC
CCGTTCGTTTTCCCCGGGTGGCGTACCCAATTCCAAAGTGATCGAATACTACCGTCGTCGTGCGGCGGCCGGTGTCGGCC
TGATCATCACTGAAGGCACCACCGTTGGCCACCAGGCTTCCAACGGTTACCCGAACGTGCCGCACTTCTACGGTGAAGCG
GCGTTGGCCGGCTGGAAAAAAGTCGTGGAGGCGGTACACGCAGAAGGCGGCAAGATCGTTCCGCAACTGTGGCACGTCGG
CAATGTGCGCCGTCTGGGCACCGAGCCAGACGCGAGCGTGCCGGGTTACGGCCCGTCGGAAAAACTCAAGGACGGTCAGG
TCGTCGTACACCGCATGACCAAACAGGACATCCAGGACGTGATCGCAGCCTTCGCCCAAGCGGCGAAAGATGCCCAAAGC
ATCGGCATGGACGGCGTGGAAATCCACGGCGCCCACGGTTACCTGATCGACCAGTTCTTCTGGGAAGGCAGCAATCAGCG
CACTGACGAATACGGTGGCAGCCTGGCCAACCGTTCGCGTTTCGCCATCGAGCTGATCCAGGCCGTACGCGCCGCTGTTG
GCGAAGGCTTCCCGATCATCTTCCGCTTCTCGCAGTGGAAACAGCAGGACTACACCGCGCGTCTGGTGCAAACCGCCGAA
GCGCTGGGCGAATTCCTCAAGCCGTTGTCCGACGCAGGCGTGGACATTTTCCACTGCTCGACGCGGCGTTTCTGGGAGCC
GGAATTCGACGGTTCCGACCTGAACCTGGCCGGCTGGACACGCAAACTGACGGGCAAACCGACCATCACCGTCGGCAGCG
TCGGCCTCGATGGCGAGTTCCTGCAGTTCATGGTCAACACCGACAAGGTCGCGCAACCGGCCAGTCTGGAAAAACTGCTG
GAGCGTCTGAACAACGAAGAATTCGATCTGGTGGCGGTGGGGCGTGCGCTGCTGGTCGATCCGGACTGGGCGCAGAAAGT
GCGCGAAGGCCGCGAGCAGGACATTCTGCCGTTCAGCCGTGAGGCGTTGATGACGCTGGTTTGA

Upstream 100 bases:

>100_bases
ATCATTCAGATCAATAGTGCTAGCAGCTGGGTGGAACTAAACATATTATCGCCGTCATATGAATCGCATGCCCGTCGATA
ACCTTTTCGTGGAGCCTCCC

Downstream 100 bases:

>100_bases
TCGTCGTGGGAGGCTTGTCGCGCAGGTCGCGGCAAGCCCCCAGCAAATGGTTTTCAAATTGTTCGACGATAGCTGCCCAA
CCCTGGCGGCTGGCGTGTTG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MPVQALFKPFQLGALELPTRVVMAPMTRSFSPGGVPNSKVIEYYRRRAAAGVGLIITEGTTVGHQASNGYPNVPHFYGEA
ALAGWKKVVEAVHAEGGKIVPQLWHVGNVRRLGTEPDASVPGYGPSEKLKDGQVVVHRMTKQDIQDVIAAFAQAAKDAQS
IGMDGVEIHGAHGYLIDQFFWEGSNQRTDEYGGSLANRSRFAIELIQAVRAAVGEGFPIIFRFSQWKQQDYTARLVQTAE
ALGEFLKPLSDAGVDIFHCSTRRFWEPEFDGSDLNLAGWTRKLTGKPTITVGSVGLDGEFLQFMVNTDKVAQPASLEKLL
ERLNNEEFDLVAVGRALLVDPDWAQKVREGREQDILPFSREALMTLV

Sequences:

>Translated_367_residues
MPVQALFKPFQLGALELPTRVVMAPMTRSFSPGGVPNSKVIEYYRRRAAAGVGLIITEGTTVGHQASNGYPNVPHFYGEA
ALAGWKKVVEAVHAEGGKIVPQLWHVGNVRRLGTEPDASVPGYGPSEKLKDGQVVVHRMTKQDIQDVIAAFAQAAKDAQS
IGMDGVEIHGAHGYLIDQFFWEGSNQRTDEYGGSLANRSRFAIELIQAVRAAVGEGFPIIFRFSQWKQQDYTARLVQTAE
ALGEFLKPLSDAGVDIFHCSTRRFWEPEFDGSDLNLAGWTRKLTGKPTITVGSVGLDGEFLQFMVNTDKVAQPASLEKLL
ERLNNEEFDLVAVGRALLVDPDWAQKVREGREQDILPFSREALMTLV
>Mature_366_residues
PVQALFKPFQLGALELPTRVVMAPMTRSFSPGGVPNSKVIEYYRRRAAAGVGLIITEGTTVGHQASNGYPNVPHFYGEAA
LAGWKKVVEAVHAEGGKIVPQLWHVGNVRRLGTEPDASVPGYGPSEKLKDGQVVVHRMTKQDIQDVIAAFAQAAKDAQSI
GMDGVEIHGAHGYLIDQFFWEGSNQRTDEYGGSLANRSRFAIELIQAVRAAVGEGFPIIFRFSQWKQQDYTARLVQTAEA
LGEFLKPLSDAGVDIFHCSTRRFWEPEFDGSDLNLAGWTRKLTGKPTITVGSVGLDGEFLQFMVNTDKVAQPASLEKLLE
RLNNEEFDLVAVGRALLVDPDWAQKVREGREQDILPFSREALMTLV

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=224, Percent_Identity=37.5, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1789463, Length=371, Percent_Identity=29.9191374663073, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17559802, Length=331, Percent_Identity=27.190332326284, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559804, Length=349, Percent_Identity=26.0744985673352, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=198, Percent_Identity=29.2929292929293, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=247, Percent_Identity=29.5546558704453, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565138, Length=209, Percent_Identity=31.1004784688995, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=68, Percent_Identity=50, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17566914, Length=196, Percent_Identity=30.6122448979592, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17566916, Length=262, Percent_Identity=26.3358778625954, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321973, Length=222, Percent_Identity=39.6396396396396, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6325086, Length=222, Percent_Identity=37.8378378378378, Blast_Score=144, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40394; Mature: 40263

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVQALFKPFQLGALELPTRVVMAPMTRSFSPGGVPNSKVIEYYRRRAAAGVGLIITEGT
CCHHHHHCHHHCCCHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCC
TVGHQASNGYPNVPHFYGEAALAGWKKVVEAVHAEGGKIVPQLWHVGNVRRLGTEPDASV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCC
PGYGPSEKLKDGQVVVHRMTKQDIQDVIAAFAQAAKDAQSIGMDGVEIHGAHGYLIDQFF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
WEGSNQRTDEYGGSLANRSRFAIELIQAVRAAVGEGFPIIFRFSQWKQQDYTARLVQTAE
HCCCCCCCHHHCCCHHCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
ALGEFLKPLSDAGVDIFHCSTRRFWEPEFDGSDLNLAGWTRKLTGKPTITVGSVGLDGEF
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCHHH
LQFMVNTDKVAQPASLEKLLERLNNEEFDLVAVGRALLVDPDWAQKVREGREQDILPFSR
HHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCEEEECCHHHHHHHCCCCCCCCCCCH
EALMTLV
HHHHHCC
>Mature Secondary Structure 
PVQALFKPFQLGALELPTRVVMAPMTRSFSPGGVPNSKVIEYYRRRAAAGVGLIITEGT
CHHHHHCHHHCCCHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCC
TVGHQASNGYPNVPHFYGEAALAGWKKVVEAVHAEGGKIVPQLWHVGNVRRLGTEPDASV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCC
PGYGPSEKLKDGQVVVHRMTKQDIQDVIAAFAQAAKDAQSIGMDGVEIHGAHGYLIDQFF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
WEGSNQRTDEYGGSLANRSRFAIELIQAVRAAVGEGFPIIFRFSQWKQQDYTARLVQTAE
HCCCCCCCHHHCCCHHCCHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
ALGEFLKPLSDAGVDIFHCSTRRFWEPEFDGSDLNLAGWTRKLTGKPTITVGSVGLDGEF
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEECCCCCHHH
LQFMVNTDKVAQPASLEKLLERLNNEEFDLVAVGRALLVDPDWAQKVREGREQDILPFSR
HHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCEEEECCHHHHHHHCCCCCCCCCCCH
EALMTLV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]