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The map label for this gene is btuE [H]

Identifier: 77457110

GI number: 77457110

Start: 1030779

End: 1031261

Strand: Direct

Name: btuE [H]

Synonym: Pfl01_0883

Alternate gene names: 77457110

Gene position: 1030779-1031261 (Clockwise)

Preceding gene: 77457109

Following gene: 77457111

Centisome position: 16.01

GC content: 57.76

Gene sequence:

>483_bases
ATGAGTGCTTTTCACGACCTTAAGTTGACAGCCCTGGATGGTCAGGAGCTACCGCTGGCACCCTTCAAGGGCCAAGTCGT
GCTGGTGGTCAACGTCGCCTCCAAATGCGGCTTGACCCCACAGTACGCGGCGCTGGAAAACCTTTATCAGCAATACAAAG
GCAAAGGCTTCAGTGTGCTGGGCCTGCCGTGCAACCAGTTTGCCGGTCAGGAACCGGGCAGTGAGCAGGAGATCCAGGAA
TTCTGCAGCCTCAACTATGGCGTGAGTTTTCCGTTGTCGAAAAAGCTCGAAGTCAATGGCCACGACCGTCATCAGCTGTA
CCGTCTGCTGGCGGGCGAGGGCGCGGAGTTTCCCGGTGACATCACCTGGAATTTCGAGAAATTCCTGCTCGGCAAGGACG
GTCGGGTACTGGCCCGGTTCTCGCCGCGCACGGCGCCGGATGATCCTTCGGTGATTACGGCGATTGAAAAAGCCCTGAGC
TGA

Upstream 100 bases:

>100_bases
ACTGGAGAGGCGCCCGAGGGCGCCTTTTTAGTCCGCGGCTGTCCTTGGTGGCCTCGCCAAGTGGCTGTTTCGAGTAGAAC
ACGGGAATCTGGAGTTCGTC

Downstream 100 bases:

>100_bases
AACACCAAAATCGCAGCCCTCGAGCTGCGATTTTTTTCGCCTGCCTTTTGACCCTTAATCATTCAGATCAATAGTGCTAG
CAGCTGGGTGGAACTAAACA

Product: glutathione peroxidase

Products: glutathione disulfide; H2O

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MSAFHDLKLTALDGQELPLAPFKGQVVLVVNVASKCGLTPQYAALENLYQQYKGKGFSVLGLPCNQFAGQEPGSEQEIQE
FCSLNYGVSFPLSKKLEVNGHDRHQLYRLLAGEGAEFPGDITWNFEKFLLGKDGRVLARFSPRTAPDDPSVITAIEKALS

Sequences:

>Translated_160_residues
MSAFHDLKLTALDGQELPLAPFKGQVVLVVNVASKCGLTPQYAALENLYQQYKGKGFSVLGLPCNQFAGQEPGSEQEIQE
FCSLNYGVSFPLSKKLEVNGHDRHQLYRLLAGEGAEFPGDITWNFEKFLLGKDGRVLARFSPRTAPDDPSVITAIEKALS
>Mature_159_residues
SAFHDLKLTALDGQELPLAPFKGQVVLVVNVASKCGLTPQYAALENLYQQYKGKGFSVLGLPCNQFAGQEPGSEQEIQEF
CSLNYGVSFPLSKKLEVNGHDRHQLYRLLAGEGAEFPGDITWNFEKFLLGKDGRVLARFSPRTAPDDPSVITAIEKALS

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=159, Percent_Identity=38.3647798742138, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI75709200, Length=148, Percent_Identity=39.1891891891892, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI90903240, Length=149, Percent_Identity=38.9261744966443, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI192455698, Length=143, Percent_Identity=35.6643356643357, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI90903238, Length=130, Percent_Identity=39.2307692307692, Blast_Score=91, Evalue=5e-19,
Organism=Homo sapiens, GI4557629, Length=167, Percent_Identity=36.5269461077844, Blast_Score=87, Evalue=5e-18,
Organism=Homo sapiens, GI6006001, Length=159, Percent_Identity=37.7358490566038, Blast_Score=87, Evalue=6e-18,
Organism=Homo sapiens, GI32967607, Length=170, Percent_Identity=32.9411764705882, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI33186887, Length=148, Percent_Identity=37.1621621621622, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI41406084, Length=159, Percent_Identity=35.2201257861635, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1788003, Length=170, Percent_Identity=48.2352941176471, Blast_Score=171, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17506887, Length=150, Percent_Identity=43.3333333333333, Blast_Score=134, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17535473, Length=150, Percent_Identity=42.6666666666667, Blast_Score=128, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71993584, Length=139, Percent_Identity=41.0071942446043, Blast_Score=113, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI193210707, Length=150, Percent_Identity=37.3333333333333, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17550320, Length=180, Percent_Identity=31.1111111111111, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI193209493, Length=181, Percent_Identity=30.939226519337, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319721, Length=147, Percent_Identity=51.7006802721088, Blast_Score=166, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6322228, Length=161, Percent_Identity=50.3105590062112, Blast_Score=165, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6322826, Length=148, Percent_Identity=39.8648648648649, Blast_Score=125, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24656767, Length=165, Percent_Identity=36.969696969697, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24656777, Length=165, Percent_Identity=36.969696969697, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24656772, Length=165, Percent_Identity=36.969696969697, Blast_Score=99, Evalue=9e-22,
Organism=Drosophila melanogaster, GI85726443, Length=146, Percent_Identity=36.986301369863, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: 1.11.1.9

Molecular weight: Translated: 17546; Mature: 17415

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFHDLKLTALDGQELPLAPFKGQVVLVVNVASKCGLTPQYAALENLYQQYKGKGFSVL
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCEEE
GLPCNQFAGQEPGSEQEIQEFCSLNYGVSFPLSKKLEVNGHDRHQLYRLLAGEGAEFPGD
CCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCC
ITWNFEKFLLGKDGRVLARFSPRTAPDDPSVITAIEKALS
EEECHHEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SAFHDLKLTALDGQELPLAPFKGQVVLVVNVASKCGLTPQYAALENLYQQYKGKGFSVL
CCCCCEEEEEECCCCCCCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHCCCCCEEE
GLPCNQFAGQEPGSEQEIQEFCSLNYGVSFPLSKKLEVNGHDRHQLYRLLAGEGAEFPGD
CCCHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCC
ITWNFEKFLLGKDGRVLARFSPRTAPDDPSVITAIEKALS
EEECHHEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glutathione; H2O2

Specific reaction: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]