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The map label for this gene is waaA [H]

Identifier: 77456718

GI number: 77456718

Start: 566922

End: 568202

Strand: Direct

Name: waaA [H]

Synonym: Pfl01_0490

Alternate gene names: 77456718

Gene position: 566922-568202 (Clockwise)

Preceding gene: 77456717

Following gene: 77456720

Centisome position: 8.81

GC content: 63.93

Gene sequence:

>1281_bases
ATGAATAGAACTCTCTACACCGCGCTGTTTTACCTGGGGCTGCCATTGGTGGCGATTCGGCTGTGGCTGCGTTCGCGCAA
GGCGCCGGCGTATGCCGAGCGGATTGGCGAACGTTTCTCCTGTGGCATGCCGGCCCTGCAACCGGGCGGCATCTGGGTTC
ACGCCGTGTCGGTGGGCGAAAGCATCGCCGCCGCGCCGATGATTCGTGCACTGCTGCAACGTTATCCACAGCTGCCGATC
ACCGTGACTTGCATGACGCCGACCGGTTCGGAACGCATCCTGGCGATGTTCGCCAACGAGCCGCGCATCCAGCATTGCTA
CCTGCCTTACGATTTGCCCTGCGCGGCGGCGCGTTTTCTGGATCGCGTGCAGCCGAAACTGGCAGTGATCATGGAAACCG
AACTCTGGCCGAACCACATCCACCAGTGCGCCAAGCGCGGGATTCCGGTGGCGTTGGCCAACGGGCGGCTGTCCGAGCGT
TCGGCCAAGGGCTATGGCCGGTTCAGCAAACTGACCGCGCCGATGCTCGCCGAAATGAGCCTGTTCGCCGTGCAGACCGA
GGCCGAAGCACAGCGCTTTCGCGATCTCGGTGCGCGCCCGGAGACAGTCGAAGTCACCGGCTCGATCAAGTTCGACCTGA
CCATCGACCCGCAACTGCTGCAACGTGCCCATGAATTGCGCGGCCAATGGCAGGCGCTGGAGCGTCCGGTGTGGATCGCC
GCCAGCACCCATGAAGGCGAAGACGAAGTGGTGCTGAGCGCTCACCGTCGTTTGCTGGCCAACCATCCCGATGCGCTGCT
GATTCTGGTGCCGCGCCATCCGGAGCGTTTCAACTCGGTGTTCGAGTTGTGCCAGCGTGAAGGTTTTGCCACGGTGCGTC
GTTCCACTGGCGCGAGCGTCGATGCGCAGACTTCGGTGCTGCTCGGCGACACCATGGGCGAGTTGCTGTTTCTCTACGCC
CTGGCCGACAGCGCGTTTGTTGGCGGCAGTCTGGTGCCCAACGGCGGACACAATCTGCTGGAACCGGCAGCCTTGGCGAA
ACCGGTGATCAGCGGGCCGCACCTGTTCAACTTCCTCGACATCGCCGCGCAACTGCGCAGTGCCGGGGCGTTGGCCGAGG
TGGATGACGCCGAAGGTCTGGCCACAGAGGTGCAGCGCCTGTTCGAATTGCCCCGCGACGCACAGCGCATGGCCGACGCC
GGGCTGAGCGTGATGCGCCGCAATCAGGGCGCGTTGCAGCGCTTGCTCGATGGACTGGGAAGGTTGATCGACCGCCCATA
A

Upstream 100 bases:

>100_bases
GCCGCGACGAACCGCTTGGCCCGGCCGCTCGCTGGCTGGCGGAGCGATTTGCCATACACCTGCGGGCGATCGGCGGCAAA
AGCCGATAAACTCCGCCGCC

Downstream 100 bases:

>100_bases
AAAAACGCAGCCTCAGGGCTGCGTTTCTTGTTTAAGGCCGGGCCTTGAGCTGGGCTTCGGCAGCCTTGGCCAGATCCGGC
GGCAGGAAATCCTTGTCCGG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNRTLYTALFYLGLPLVAIRLWLRSRKAPAYAERIGERFSCGMPALQPGGIWVHAVSVGESIAAAPMIRALLQRYPQLPI
TVTCMTPTGSERILAMFANEPRIQHCYLPYDLPCAAARFLDRVQPKLAVIMETELWPNHIHQCAKRGIPVALANGRLSER
SAKGYGRFSKLTAPMLAEMSLFAVQTEAEAQRFRDLGARPETVEVTGSIKFDLTIDPQLLQRAHELRGQWQALERPVWIA
ASTHEGEDEVVLSAHRRLLANHPDALLILVPRHPERFNSVFELCQREGFATVRRSTGASVDAQTSVLLGDTMGELLFLYA
LADSAFVGGSLVPNGGHNLLEPAALAKPVISGPHLFNFLDIAAQLRSAGALAEVDDAEGLATEVQRLFELPRDAQRMADA
GLSVMRRNQGALQRLLDGLGRLIDRP

Sequences:

>Translated_426_residues
MNRTLYTALFYLGLPLVAIRLWLRSRKAPAYAERIGERFSCGMPALQPGGIWVHAVSVGESIAAAPMIRALLQRYPQLPI
TVTCMTPTGSERILAMFANEPRIQHCYLPYDLPCAAARFLDRVQPKLAVIMETELWPNHIHQCAKRGIPVALANGRLSER
SAKGYGRFSKLTAPMLAEMSLFAVQTEAEAQRFRDLGARPETVEVTGSIKFDLTIDPQLLQRAHELRGQWQALERPVWIA
ASTHEGEDEVVLSAHRRLLANHPDALLILVPRHPERFNSVFELCQREGFATVRRSTGASVDAQTSVLLGDTMGELLFLYA
LADSAFVGGSLVPNGGHNLLEPAALAKPVISGPHLFNFLDIAAQLRSAGALAEVDDAEGLATEVQRLFELPRDAQRMADA
GLSVMRRNQGALQRLLDGLGRLIDRP
>Mature_426_residues
MNRTLYTALFYLGLPLVAIRLWLRSRKAPAYAERIGERFSCGMPALQPGGIWVHAVSVGESIAAAPMIRALLQRYPQLPI
TVTCMTPTGSERILAMFANEPRIQHCYLPYDLPCAAARFLDRVQPKLAVIMETELWPNHIHQCAKRGIPVALANGRLSER
SAKGYGRFSKLTAPMLAEMSLFAVQTEAEAQRFRDLGARPETVEVTGSIKFDLTIDPQLLQRAHELRGQWQALERPVWIA
ASTHEGEDEVVLSAHRRLLANHPDALLILVPRHPERFNSVFELCQREGFATVRRSTGASVDAQTSVLLGDTMGELLFLYA
LADSAFVGGSLVPNGGHNLLEPAALAKPVISGPHLFNFLDIAAQLRSAGALAEVDDAEGLATEVQRLFELPRDAQRMADA
GLSVMRRNQGALQRLLDGLGRLIDRP

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=422, Percent_Identity=54.739336492891, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46930; Mature: 46930

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTLYTALFYLGLPLVAIRLWLRSRKAPAYAERIGERFSCGMPALQPGGIWVHAVSVGE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCC
SIAAAPMIRALLQRYPQLPITVTCMTPTGSERILAMFANEPRIQHCYLPYDLPCAAARFL
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHH
DRVQPKLAVIMETELWPNHIHQCAKRGIPVALANGRLSERSAKGYGRFSKLTAPMLAEMS
HHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCHHCCCCCCHHHHHHHHHHHHHH
LFAVQTEAEAQRFRDLGARPETVEVTGSIKFDLTIDPQLLQRAHELRGQWQALERPVWIA
HHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEE
ASTHEGEDEVVLSAHRRLLANHPDALLILVPRHPERFNSVFELCQREGFATVRRSTGASV
ECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
DAQTSVLLGDTMGELLFLYALADSAFVGGSLVPNGGHNLLEPAALAKPVISGPHLFNFLD
CCCCCEEEHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHH
IAAQLRSAGALAEVDDAEGLATEVQRLFELPRDAQRMADAGLSVMRRNQGALQRLLDGLG
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RLIDRP
HHHCCC
>Mature Secondary Structure
MNRTLYTALFYLGLPLVAIRLWLRSRKAPAYAERIGERFSCGMPALQPGGIWVHAVSVGE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCC
SIAAAPMIRALLQRYPQLPITVTCMTPTGSERILAMFANEPRIQHCYLPYDLPCAAARFL
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCHHHHHHHH
DRVQPKLAVIMETELWPNHIHQCAKRGIPVALANGRLSERSAKGYGRFSKLTAPMLAEMS
HHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCHHCCCCCCHHHHHHHHHHHHHH
LFAVQTEAEAQRFRDLGARPETVEVTGSIKFDLTIDPQLLQRAHELRGQWQALERPVWIA
HHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEE
ASTHEGEDEVVLSAHRRLLANHPDALLILVPRHPERFNSVFELCQREGFATVRRSTGASV
ECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
DAQTSVLLGDTMGELLFLYALADSAFVGGSLVPNGGHNLLEPAALAKPVISGPHLFNFLD
CCCCCEEEHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHH
IAAQLRSAGALAEVDDAEGLATEVQRLFELPRDAQRMADAGLSVMRRNQGALQRLLDGLG
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RLIDRP
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]