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The map label for this gene is ynfL [H]

Identifier: 77456717

GI number: 77456717

Start: 566008

End: 566910

Strand: Direct

Name: ynfL [H]

Synonym: Pfl01_0489

Alternate gene names: 77456717

Gene position: 566008-566910 (Clockwise)

Preceding gene: 77456712

Following gene: 77456718

Centisome position: 8.79

GC content: 64.45

Gene sequence:

>903_bases
ATGAGCATGCAGTGGAATCTGGAGCAATTGCGGGTGTTCGTCGAGGTGGCCGAACAGCGTTCGTTCTCCGCCGTGGCGCG
GCAGCAACGCAAGGCACAGTCGGCGATCAGCAGCGCGATTGCCATGCTCGAGGACGATCTCGGCGTGAGTCTGTTCGAGC
GTAGCAGCGGCCGCCAGCCGAGTCTTACCGAAGCGGGGGAGGCGTTGCTGGAAGAGGCGCGGGAAGTGTTGCGCCAATGC
GAGCGCCTGAACGGCCGGGCCGTGGCCATGATGCGCGGGCAGGAAGCGCAATTGCGGGTGGCCCAGGACGAGGCGATGCC
CTATCAGGCGCTGGTGGAAAGTTTCGGCGCCCTGGCCGAGCAGTTTCCCAGTCTTGAAGTCCAGTTGACCAGCGCCGCCC
AGGGCGAGGTTTCGCGCAAGCTAGTGGAGCGCCGGGCCGATCTCGGGTTGTTGTTCTATCACGATGAAATCCCTGAAGCG
CTGGAGCGCCGGGTGCTGGGCAGTGTGGAAATGGTCACAGTCTGCGGCGTCAACCATCCCTTGGCATCGCAGGCTTACGT
CACCTGCCAGCAACTGGCTCAGCATCGGCAGTTGTTGATGTCGACCCAGACCAGCATTTATCCCGGCAACGAACCGGCCA
GTCCGCAGGTGTGGCGCGCTGACAGCTTCTATGTGATGGCCGAATGGCTGGTGCGCGATCTCGGCTGGGCCTGGCTGCCG
CGTCACGTGGTGCAGTACTCGGCGTATCAGGGGCTGATGGTGGAGCTCGACAGCGAATGGACGCCGCCGGCGCTGGTGGT
GGAACTGGTCTGGCGCCGCGACGAACCGCTTGGCCCGGCCGCTCGCTGGCTGGCGGAGCGATTTGCCATACACCTGCGGG
CGATCGGCGGCAAAAGCCGATAA

Upstream 100 bases:

>100_bases
TCAAATCCTCGTGTGAAGCGTTGCTTTCTGAGGCGGCATTCTAGTGACTCCCCAGATGGGGTAAAGTCATTACCTATCTG
TTTACAAGATGGGTTGGCCG

Downstream 100 bases:

>100_bases
ACTCCGCCGCCATGAATAGAACTCTCTACACCGCGCTGTTTTACCTGGGGCTGCCATTGGTGGCGATTCGGCTGTGGCTG
CGTTCGCGCAAGGCGCCGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSMQWNLEQLRVFVEVAEQRSFSAVARQQRKAQSAISSAIAMLEDDLGVSLFERSSGRQPSLTEAGEALLEEAREVLRQC
ERLNGRAVAMMRGQEAQLRVAQDEAMPYQALVESFGALAEQFPSLEVQLTSAAQGEVSRKLVERRADLGLLFYHDEIPEA
LERRVLGSVEMVTVCGVNHPLASQAYVTCQQLAQHRQLLMSTQTSIYPGNEPASPQVWRADSFYVMAEWLVRDLGWAWLP
RHVVQYSAYQGLMVELDSEWTPPALVVELVWRRDEPLGPAARWLAERFAIHLRAIGGKSR

Sequences:

>Translated_300_residues
MSMQWNLEQLRVFVEVAEQRSFSAVARQQRKAQSAISSAIAMLEDDLGVSLFERSSGRQPSLTEAGEALLEEAREVLRQC
ERLNGRAVAMMRGQEAQLRVAQDEAMPYQALVESFGALAEQFPSLEVQLTSAAQGEVSRKLVERRADLGLLFYHDEIPEA
LERRVLGSVEMVTVCGVNHPLASQAYVTCQQLAQHRQLLMSTQTSIYPGNEPASPQVWRADSFYVMAEWLVRDLGWAWLP
RHVVQYSAYQGLMVELDSEWTPPALVVELVWRRDEPLGPAARWLAERFAIHLRAIGGKSR
>Mature_299_residues
SMQWNLEQLRVFVEVAEQRSFSAVARQQRKAQSAISSAIAMLEDDLGVSLFERSSGRQPSLTEAGEALLEEAREVLRQCE
RLNGRAVAMMRGQEAQLRVAQDEAMPYQALVESFGALAEQFPSLEVQLTSAAQGEVSRKLVERRADLGLLFYHDEIPEAL
ERRVLGSVEMVTVCGVNHPLASQAYVTCQQLAQHRQLLMSTQTSIYPGNEPASPQVWRADSFYVMAEWLVRDLGWAWLPR
HVVQYSAYQGLMVELDSEWTPPALVVELVWRRDEPLGPAARWLAERFAIHLRAIGGKSR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=193, Percent_Identity=29.0155440414508, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI145693105, Length=299, Percent_Identity=24.4147157190635, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082024, Length=256, Percent_Identity=25.78125, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787530, Length=191, Percent_Identity=25.6544502617801, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786713, Length=178, Percent_Identity=30.8988764044944, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788887, Length=288, Percent_Identity=25.3472222222222, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=242, Percent_Identity=28.5123966942149, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789639, Length=208, Percent_Identity=28.3653846153846, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789492, Length=291, Percent_Identity=25.4295532646048, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=146, Percent_Identity=29.4520547945205, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=148, Percent_Identity=31.0810810810811, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=27.8688524590164, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=289, Percent_Identity=26.643598615917, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33809; Mature: 33678

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMQWNLEQLRVFVEVAEQRSFSAVARQQRKAQSAISSAIAMLEDDLGVSLFERSSGRQP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
SLTEAGEALLEEAREVLRQCERLNGRAVAMMRGQEAQLRVAQDEAMPYQALVESFGALAE
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHEEEHHHHCCCHHHHHHHHHHHHH
QFPSLEVQLTSAAQGEVSRKLVERRADLGLLFYHDEIPEALERRVLGSVEMVTVCGVNHP
HCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCHHEEEEECCCCC
LASQAYVTCQQLAQHRQLLMSTQTSIYPGNEPASPQVWRADSFYVMAEWLVRDLGWAWLP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCHHHHH
RHVVQYSAYQGLMVELDSEWTPPALVVELVWRRDEPLGPAARWLAERFAIHLRAIGGKSR
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SMQWNLEQLRVFVEVAEQRSFSAVARQQRKAQSAISSAIAMLEDDLGVSLFERSSGRQP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
SLTEAGEALLEEAREVLRQCERLNGRAVAMMRGQEAQLRVAQDEAMPYQALVESFGALAE
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHEEEHHHHCCCHHHHHHHHHHHHH
QFPSLEVQLTSAAQGEVSRKLVERRADLGLLFYHDEIPEALERRVLGSVEMVTVCGVNHP
HCCCCEEEECCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCHHEEEEECCCCC
LASQAYVTCQQLAQHRQLLMSTQTSIYPGNEPASPQVWRADSFYVMAEWLVRDLGWAWLP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCHHHHH
RHVVQYSAYQGLMVELDSEWTPPALVVELVWRRDEPLGPAARWLAERFAIHLRAIGGKSR
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]