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The map label for this gene is thiC [H]

Identifier: 77456720

GI number: 77456720

Start: 570117

End: 572006

Strand: Direct

Name: thiC [H]

Synonym: Pfl01_0492

Alternate gene names: 77456720

Gene position: 570117-572006 (Clockwise)

Preceding gene: 77456718

Following gene: 77456721

Centisome position: 8.85

GC content: 61.22

Gene sequence:

>1890_bases
ATGACTACAAAATCTAAAAATGCGATCAACCTGAGTGACTCGGCCAAGGTCGACGAGCAGTCGGTCCAACCGTTCACCCG
TTCGCAAAAAGTCTACGTTCAGGGCTCCCGCCCGGACATCCGCGTGCCGATGCGCGAAATCACCCTCGATGTGACCCCGA
CCGACTTTGGCGGCGAAATCAATGCGCCGGTCACCGTCTACGACACCTCGGGCCCGTACACCGATCCGAACGTGGTGATC
GACGTGCGCAAAGGTCTGGGCGACGTGCGTTCGGCGTGGATCGACGACCGTGGCGACACCGAGCGTCTGCCAGGCCTGAG
TTCCAACTTCGGCCAGGAACGCCTCGCCGACCCAGAGCTGACCAAACTGCGTTTTGCCCACGTCAACAACCCGCGCCGGG
CCAAGGCCGGGGCCAACGTCAGCCAGATGCACTACGCGCGCAAAGGCATCATCACCGCCGAGATGGAATACGTCGCCATC
CGCGAAAACATGAAGCTGCAAGAGGCCCGCGCCGCCGGCCTGTTGAAGCAGCAACATGCCGGCCACAGCTTCGGCGCGAG
CATCCCGAAAGAAATCACCCCTGAATTCGTGCGCGAAGAAATCGCCCGCGGCCGCGCGATCATCCCGGCCAACATCAACC
ACGTCGAACTGGAACCGATGATCATCGGTCGCAACTTCCTGGTGAAGATCAACGGCAACATCGGCAACAGCGCGCTGGGT
TCGTCCATCGAAGAAGAAGTGGCGAAACTGACCTGGGGCATTCGCTGGGGCTCGGACACGGTGATGGACTTGTCCACCGG
CAAGCACATTCACGAAACCCGCGAGTGGATCATCCGTAACTCGCCAGTGCCGATCGGTACGGTGCCGATTTATCAGGCGC
TGGAAAAAGTGAATGGCGTCGCCGAGGACCTGACCTGGGAGCTGTTCCGCGACACGCTGATCGAGCAGGCAGAGCAGGGC
GTCGACTACTTCACCATCCACGCCGGCGTGTTGCTGCGCTACGTGCCGCTGACCGCCAAGCGCGTGACCGGCATCGTTTC
CCGTGGCGGTTCGATCATGGCCAAGTGGTGCCTGGCGCACCACAAAGAGAACTTCCTCTACACCCACTTCGACGAAATCT
GCGAAATCATGAAGGCCTACGACGTCAGCTTCTCGCTGGGCGATGGTCTGCGTCCGGGCTCGATTGCCGACGCCAACGAC
GAAGCGCAATTCGGCGAACTGGAAACCCTCGGTGAGCTGACCAAGATCGCCTGGAAGCACGACGTGCAATGCATGATCGA
AGGCCCGGGCCACGTGCCGATGCAGCTGATCAAAGAGAACATGGACAAGCAGCTGGAGTGCTGCGACGAGGCGCCGTTCT
ACACCCTCGGCCCGCTGACCACCGACATTGCGCCGGGCTACGACCACATCACCTCCGGCATCGGTGCGGCGATGATCGGC
TGGTTCGGTTGCGCGATGCTCTGCTACGTGACGCCGAAGGAGCACCTGGGTCTGCCGAACAAGGATGACGTGAAGACCGG
GATCATCACCTACAAGATCGCCGCTCACGCCGCGGACCTGGCCAAGGGGCATCCGGGCGCGCAGATCCGCGACAACGCCT
TGAGCAAGGCGCGTTTCGAATTCCGTTGGGAAGACCAGTTCAACCTCGGTCTGGACCCGGACACCGCTCGTTCGTATCAC
GATGAAACCTTGCCGAAGGACTCGGCGAAGGTCGCGCATTTCTGTTCGATGTGCGGGCCGAAGTTCTGCTCGATGAAGAT
CACCCAGGAAGTTCGCGAATACGCGGCCAACCAGCGGATCGACGCGGTCGACGTGGACGTCGCCCAGGGCCTGGCGGAAC
AGGCCGAGCGGTTCAAGAAGGAAGGCAGTCAGCTGTACAAGAAGGTTTGA

Upstream 100 bases:

>100_bases
ACGCTCGACGACGATGCACAGCACCCGTTCTGGGTGCGTCCGTGCCTTTCAGGTTCTGCTCCGACAATCCACTGCCTGGA
TGCTGTCTGGAGAGCCCGTG

Downstream 100 bases:

>100_bases
TCCCGCACTGACCGTTCCCACGTCTAGGCGTCGGACCGTCCGCACCGATCGTTCCCACGCAGAGCGTGGGAACGATCAAC
GGGTCAGCACAACAACAAAT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MTTKSKNAINLSDSAKVDEQSVQPFTRSQKVYVQGSRPDIRVPMREITLDVTPTDFGGEINAPVTVYDTSGPYTDPNVVI
DVRKGLGDVRSAWIDDRGDTERLPGLSSNFGQERLADPELTKLRFAHVNNPRRAKAGANVSQMHYARKGIITAEMEYVAI
RENMKLQEARAAGLLKQQHAGHSFGASIPKEITPEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALG
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWELFRDTLIEQAEQG
VDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGSIADAND
EAQFGELETLGELTKIAWKHDVQCMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARSYH
DETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRIDAVDVDVAQGLAEQAERFKKEGSQLYKKV

Sequences:

>Translated_629_residues
MTTKSKNAINLSDSAKVDEQSVQPFTRSQKVYVQGSRPDIRVPMREITLDVTPTDFGGEINAPVTVYDTSGPYTDPNVVI
DVRKGLGDVRSAWIDDRGDTERLPGLSSNFGQERLADPELTKLRFAHVNNPRRAKAGANVSQMHYARKGIITAEMEYVAI
RENMKLQEARAAGLLKQQHAGHSFGASIPKEITPEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALG
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWELFRDTLIEQAEQG
VDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGSIADAND
EAQFGELETLGELTKIAWKHDVQCMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARSYH
DETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRIDAVDVDVAQGLAEQAERFKKEGSQLYKKV
>Mature_628_residues
TTKSKNAINLSDSAKVDEQSVQPFTRSQKVYVQGSRPDIRVPMREITLDVTPTDFGGEINAPVTVYDTSGPYTDPNVVID
VRKGLGDVRSAWIDDRGDTERLPGLSSNFGQERLADPELTKLRFAHVNNPRRAKAGANVSQMHYARKGIITAEMEYVAIR
ENMKLQEARAAGLLKQQHAGHSFGASIPKEITPEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALGS
SIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGVAEDLTWELFRDTLIEQAEQGV
DYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGSIADANDE
AQFGELETLGELTKIAWKHDVQCMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGW
FGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARSYHD
ETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRIDAVDVDVAQGLAEQAERFKKEGSQLYKKV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=611, Percent_Identity=72.8314238952537, Blast_Score=941, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 69812; Mature: 69681

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKSKNAINLSDSAKVDEQSVQPFTRSQKVYVQGSRPDIRVPMREITLDVTPTDFGGEI
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCHHHEEEEECCCCCCCCC
NAPVTVYDTSGPYTDPNVVIDVRKGLGDVRSAWIDDRGDTERLPGLSSNFGQERLADPEL
CCCEEEEECCCCCCCCCEEEEEHHCCHHHHHHHCCCCCCHHHCCCCCCCCCHHHCCCCCH
TKLRFAHVNNPRRAKAGANVSQMHYARKGIITAEMEYVAIRENMKLQEARAAGLLKQQHA
HHEEEEECCCCHHHHCCCCHHHHHHHHHCCEEEHHEEEEHHCCCHHHHHHHHHHHHHHHC
GHSFGASIPKEITPEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALG
CCCCCCCCCHHCCHHHHHHHHHCCCEEEECCCCEEEECEEEECCEEEEEECCCCCCHHHC
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGV
CHHHHHHHHHHHCEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCH
AEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAH
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHH
HKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGSIADANDEAQFGELETLGELTKIAWKH
HCCCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DVQCMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFE
HHHHHHHHEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCEEE
FRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRI
EEECCCCCCCCCCCHHHHHCHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
DAVDVDVAQGLAEQAERFKKEGSQLYKKV
CEEHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTKSKNAINLSDSAKVDEQSVQPFTRSQKVYVQGSRPDIRVPMREITLDVTPTDFGGEI
CCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCHHHEEEEECCCCCCCCC
NAPVTVYDTSGPYTDPNVVIDVRKGLGDVRSAWIDDRGDTERLPGLSSNFGQERLADPEL
CCCEEEEECCCCCCCCCEEEEEHHCCHHHHHHHCCCCCCHHHCCCCCCCCCHHHCCCCCH
TKLRFAHVNNPRRAKAGANVSQMHYARKGIITAEMEYVAIRENMKLQEARAAGLLKQQHA
HHEEEEECCCCHHHHCCCCHHHHHHHHHCCEEEHHEEEEHHCCCHHHHHHHHHHHHHHHC
GHSFGASIPKEITPEFVREEIARGRAIIPANINHVELEPMIIGRNFLVKINGNIGNSALG
CCCCCCCCCHHCCHHHHHHHHHCCCEEEECCCCEEEECEEEECCEEEEEECCCCCCHHHC
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGV
CHHHHHHHHHHHCEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCH
AEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAH
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHH
HKENFLYTHFDEICEIMKAYDVSFSLGDGLRPGSIADANDEAQFGELETLGELTKIAWKH
HCCCCEEECHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DVQCMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHH
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFE
HHHHHHHHEECCHHHCCCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHCEEE
FRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRI
EEECCCCCCCCCCCHHHHHCHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
DAVDVDVAQGLAEQAERFKKEGSQLYKKV
CEEHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA