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The map label for this gene is hisI

Identifier: 77456612

GI number: 77456612

Start: 437971

End: 438372

Strand: Reverse

Name: hisI

Synonym: Pfl01_0384

Alternate gene names: 77456612

Gene position: 438372-437971 (Counterclockwise)

Preceding gene: 77456613

Following gene: 77456611

Centisome position: 6.81

GC content: 58.71

Gene sequence:

>402_bases
ATGAAAAACTGGCTGGACGAGATCAAGTGGGACGCCGATGGCCTGGTGCCGGCGATTGCCCAGGATCACAAGACCGGACG
CGTATTGATGATGGCCTGGATGAACCGCGAAGCCCTGGAGCTGACCGCGGCGGAAAACCGTGCCATCTATTGGTCACGTT
CCCGTGGCAAGCTGTGGCGCAAGGGCGAAGAGTCCGGCCACGTACAGACGCTGCATGAAATGCGTCTGGACTGCGACGCC
GACGTCATCATCCTGATGGTCGAGCAGATCGGCGACATTGCTTGCCATACCGGCCGTCAAAGCTGCTTCTACCGCGTCTT
CGAAAACGGCGACTGGAAAACGGTCGACCCGGTCCTGAAAGACCCGCACGCTATTTACTCCGCAGGACACAAACATGAGT
GA

Upstream 100 bases:

>100_bases
ATCTGGTCGTTCGCCGATAGCCAGTTCCGTGATCGGCTGGCACACTGTTTCAACGCCTGAACCCGACTATTGAAGTGTGG
GTTCGCTGTCGGAGTCGAAG

Downstream 100 bases:

>100_bases
CACCCTGACCCGCCTCGCTCAGGTGCTGGAAGAGCGCAAGGGCGCTGCTGCCGACAGTTCGTATGTGGCTAGTCTGTATC
ACAAGGGCTTGAACAAGATT

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MKNWLDEIKWDADGLVPAIAQDHKTGRVLMMAWMNREALELTAAENRAIYWSRSRGKLWRKGEESGHVQTLHEMRLDCDA
DVIILMVEQIGDIACHTGRQSCFYRVFENGDWKTVDPVLKDPHAIYSAGHKHE

Sequences:

>Translated_133_residues
MKNWLDEIKWDADGLVPAIAQDHKTGRVLMMAWMNREALELTAAENRAIYWSRSRGKLWRKGEESGHVQTLHEMRLDCDA
DVIILMVEQIGDIACHTGRQSCFYRVFENGDWKTVDPVLKDPHAIYSAGHKHE
>Mature_133_residues
MKNWLDEIKWDADGLVPAIAQDHKTGRVLMMAWMNREALELTAAENRAIYWSRSRGKLWRKGEESGHVQTLHEMRLDCDA
DVIILMVEQIGDIACHTGRQSCFYRVFENGDWKTVDPVLKDPHAIYSAGHKHE

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=137, Percent_Identity=33.5766423357664, Blast_Score=86, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319819, Length=103, Percent_Identity=34.9514563106796, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_PSEPF (Q3KJC8)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346117.1
- ProteinModelPortal:   Q3KJC8
- SMR:   Q3KJC8
- STRING:   Q3KJC8
- GeneID:   3712647
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0384
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   PRK00051
- BioCyc:   PFLU205922:PFL_0384-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 15362; Mature: 15362

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNWLDEIKWDADGLVPAIAQDHKTGRVLMMAWMNREALELTAAENRAIYWSRSRGKLWR
CCCHHHHHCCCCCCCCHHHHCCCCCCEEEEEEECCCCCEEEEECCCCEEEEECCCCCCCC
KGEESGHVQTLHEMRLDCDADVIILMVEQIGDIACHTGRQSCFYRVFENGDWKTVDPVLK
CCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHEECCCCCEEECHHHC
DPHAIYSAGHKHE
CCHHHHCCCCCCC
>Mature Secondary Structure
MKNWLDEIKWDADGLVPAIAQDHKTGRVLMMAWMNREALELTAAENRAIYWSRSRGKLWR
CCCHHHHHCCCCCCCCHHHHCCCCCCEEEEEEECCCCCEEEEECCCCEEEEECCCCCCCC
KGEESGHVQTLHEMRLDCDADVIILMVEQIGDIACHTGRQSCFYRVFENGDWKTVDPVLK
CCCCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHEECCCCCEEECHHHC
DPHAIYSAGHKHE
CCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA