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The map label for this gene is hisF1 [H]

Identifier: 77456555

GI number: 77456555

Start: 371844

End: 372614

Strand: Direct

Name: hisF1 [H]

Synonym: Pfl01_0327

Alternate gene names: 77456555

Gene position: 371844-372614 (Clockwise)

Preceding gene: 77456554

Following gene: 77456556

Centisome position: 5.78

GC content: 61.87

Gene sequence:

>771_bases
ATGGCGCTGGCCAAACGCATCATCCCTTGCCTGGACGTGGACAACGGCCGGGTCGTCAAAGGTGTGAAGTTCGAGAACAT
CCGTGACGCCGGCGATCCGGTGGAAATCGCCCGTCGCTACGACGAGCAGGGTGCCGACGAGATTACTTTTCTCGACATCA
CCGCCAGCGTCGATGGCCGTGACACCACGCTGCACACCGTCGAGCGCATGGCCAGCCAGGTGTTCATCCCGCTGACCGTC
GGCGGTGGCGTGCGGACCGTGCAGGACATTCGCAACCTGCTCAATGCCGGTGCGGACAAGGTGTCGATCAACACCGCTGC
CGTGTTCAACCCGGAATTCGTCGGCGAAGCGGCGCAGCATTTCGGCTCGCAATGCATCGTTGTCGCCATCGACGCGAAGA
AGGTCTCCGGCCCGGGCGAAACCCCGCGCTGGGAGATCTTTACCCATGGCGGCCGCAAGCCGACCGGCCTCGACGCGGTC
GAGTGGGCGAAGAAAATGGAAGGCCTGGGTGCCGGTGAAATCCTCCTGACCAGTATGGATCAGGACGGCATGAAAAACGG
TTTCGACCTCGGCGTGACCCGCGCCATCAGCGATGCGCTGGGTATTCCGGTGATCGCGTCCGGCGGCGTCGGCAACCTGC
AGCATCTGGCTGACGGCATTCTTGAAGGTCACGCCAGCGCGGTGCTGGCGGCGAGTATTTTCCACTTCGGCGAATACACG
GTTCAAGAAGCCAAGGCTTACATGTCTAAACGCGGCATCGTAATGCGTTAA

Upstream 100 bases:

>100_bases
CATCATCGGCGCCATCACCGGCCGGGCGATCTACGAAGGCACTCTCGACGTCGCCGAAGCGCAAGCTTTCTGCGACTCGT
ACCAAGGCTGAGGACTGACC

Downstream 100 bases:

>100_bases
ACATCCATACAGGCCAGTGGACAGCATGGCGTACCCAAGGCACTCTTGGGCACGCCATGGATTTCGGTAGCCCGACATGA
TCAAACGCCTGCTTCTCGCC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MALAKRIIPCLDVDNGRVVKGVKFENIRDAGDPVEIARRYDEQGADEITFLDITASVDGRDTTLHTVERMASQVFIPLTV
GGGVRTVQDIRNLLNAGADKVSINTAAVFNPEFVGEAAQHFGSQCIVVAIDAKKVSGPGETPRWEIFTHGGRKPTGLDAV
EWAKKMEGLGAGEILLTSMDQDGMKNGFDLGVTRAISDALGIPVIASGGVGNLQHLADGILEGHASAVLAASIFHFGEYT
VQEAKAYMSKRGIVMR

Sequences:

>Translated_256_residues
MALAKRIIPCLDVDNGRVVKGVKFENIRDAGDPVEIARRYDEQGADEITFLDITASVDGRDTTLHTVERMASQVFIPLTV
GGGVRTVQDIRNLLNAGADKVSINTAAVFNPEFVGEAAQHFGSQCIVVAIDAKKVSGPGETPRWEIFTHGGRKPTGLDAV
EWAKKMEGLGAGEILLTSMDQDGMKNGFDLGVTRAISDALGIPVIASGGVGNLQHLADGILEGHASAVLAASIFHFGEYT
VQEAKAYMSKRGIVMR
>Mature_255_residues
ALAKRIIPCLDVDNGRVVKGVKFENIRDAGDPVEIARRYDEQGADEITFLDITASVDGRDTTLHTVERMASQVFIPLTVG
GGVRTVQDIRNLLNAGADKVSINTAAVFNPEFVGEAAQHFGSQCIVVAIDAKKVSGPGETPRWEIFTHGGRKPTGLDAVE
WAKKMEGLGAGEILLTSMDQDGMKNGFDLGVTRAISDALGIPVIASGGVGNLQHLADGILEGHASAVLAASIFHFGEYTV
QEAKAYMSKRGIVMR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=41.4728682170543, Blast_Score=210, Evalue=8e-56,
Organism=Escherichia coli, GI87082028, Length=247, Percent_Identity=27.1255060728745, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=36.4217252396166, Blast_Score=171, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27275; Mature: 27144

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVDNGRVVKGVKFENIRDAGDPVEIARRYDEQGADEITFLDITASVDGR
CCCHHHCCCCEECCCCCEEECEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCC
DTTLHTVERMASQVFIPLTVGGGVRTVQDIRNLLNAGADKVSINTAAVFNPEFVGEAAQH
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECEEEEECHHHHHHHHHH
FGSQCIVVAIDAKKVSGPGETPRWEIFTHGGRKPTGLDAVEWAKKMEGLGAGEILLTSMD
CCCCEEEEEEECEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEECC
QDGMKNGFDLGVTRAISDALGIPVIASGGVGNLQHLADGILEGHASAVLAASIFHFGEYT
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQEAKAYMSKRGIVMR
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALAKRIIPCLDVDNGRVVKGVKFENIRDAGDPVEIARRYDEQGADEITFLDITASVDGR
CCHHHCCCCEECCCCCEEECEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCC
DTTLHTVERMASQVFIPLTVGGGVRTVQDIRNLLNAGADKVSINTAAVFNPEFVGEAAQH
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEEECEEEEECHHHHHHHHHH
FGSQCIVVAIDAKKVSGPGETPRWEIFTHGGRKPTGLDAVEWAKKMEGLGAGEILLTSMD
CCCCEEEEEEECEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEEECC
QDGMKNGFDLGVTRAISDALGIPVIASGGVGNLQHLADGILEGHASAVLAASIFHFGEYT
CCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQEAKAYMSKRGIVMR
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]