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The map label for this gene is dctB [H]

Identifier: 77456516

GI number: 77456516

Start: 333910

End: 335724

Strand: Reverse

Name: dctB [H]

Synonym: Pfl01_0288

Alternate gene names: 77456516

Gene position: 335724-333910 (Counterclockwise)

Preceding gene: 77456517

Following gene: 77456515

Centisome position: 5.21

GC content: 65.84

Gene sequence:

>1815_bases
ATGCTCATGACTCCGACCCTACCCCGCCGCCCCCGATGGCGCAGTCTTGCCCTGCTGGCCCTGTGCCTGGCACCGCTGCT
GTGGCCGCTGGAACATCTGGCCGAGCGCTATTACCGCAGCGAACTGGCCGGCCAGAACCGTCAGACCCTCGACCTCTACG
TCGCCAACCTGCTGGGCACGCTGCATCGCTATGAAGTGCTGCCACAAATCCTCGGCGACCTGCCGGCCCTGCGCGCAGTG
CTCGGGGCGCCGGATGACGGCGTGACCCAGGGCAACGCCAACCGCCTGTTGAAGAACATCGCTGCGCAGACCGGCGCCGA
AGTCATGTACCTGATGGACACCAGCGGCCAGACGCTCGCGGCGTCGAACTGGGACAAGCACGACAGTTTCGTCGGGCGCA
ATTTCTCGTTCCGGCCGTACTTCAGCGAGGCCATGGCCGGCCGCCTCGGGCGCTTCTTCGGTCTGGGCACCACTTCCGCC
AAACGCGGCTATTTCTTCGCCGCAGCGGTGCGCAACGGCGAAAAAATCATCGGTGTACTGGTGATCAAGGTAGATCTGGA
CCACACCGAAAGCCTGTGGGGCAAAACCCCGGAACAACTGCTGGTGACCGACCACAACGGTGTCGTCATCCTCACCTCGC
GCCCGGAATGGCGCTTTCGCGCGACCCGCACCCTCAGTGATGCCGAGCGCTCGGCAATCACCGCGATCCAGCCCTACCCG
ACCCGCGAGCCACGCCCCCTGAACCTGAGCCCGACCGCGTGGCTGCCACAGACCCAGGACATCGCGGAAACCGGCTGGAG
CGTCAGCATTCTGGCCCCGCGCACGTTGATCGACCGACCGGTGCGTACCGTGGTGGCGATCGGCGGCGCCACCTTGCTGG
TAGTCATGTTGTTGCTGGGCCTGATGATGCAGCGTCGTCGTCATTACCTGGAACGGATCGCCTTCGAAGCCAAGGCCCGC
CGGGAACTCGAAGGCCGGGTTGCCGAGCGCACCAGTGATCTCGAAGGCCTCAACCGCCGACTGAAACAGGAAGTGCTTGA
ACGCGAACAGGCCCAGCAGGAACTGGTGCGGGCCCAGGACGATCTGGTACAGGCCGGCAAGCTGTCGGCGCTGGGGACGA
TGTCGGCGAGCATCAGCCACGAACTCAACCAGCCGCTGGCGGCGATCCGCAGCTACGCCGAGAACGCCGAAGTGCTGCTC
GACCACGAACGCACCGAAGACGCACGAGGCAACCTCAAACTGATCAGCGAGCTGACCGGGCGCATGGCCTCGATCATCGC
CCACCTGCGCGCCTTCGCCCGCCGCGACCGCCACGCCCCGGAAAGCGTCGCCCTGCAACCCGCGCTGGACGACGCGCTCG
CCTTGCTGGCCAAGCGTCGCCGGAGCATGGATGTCGAGCTGATCCGCGACCTGCCCGCCGCCACCCTGTGGGTCGAGGCC
GGGGAAACCCGACTGCGCCAGGTGCTGGGCAACCTGCTGGCCAACGCCCTCGACGCCCTGACCGAAAAAGGCCCGCCGCG
CAAATTGTGGCTGAGTGCCGAATCCACCGACGAAGGCGTCAACCTGTACATTCGCGACAATGGCCCGGGTTTCTGCATGG
AAGCCCTGGGCCGTGCCAGCGAACCGTTTTACACGACCAAGACCCGCACTCAGGGCCTGGGTTTAGGGTTGGCCATTTGC
GAAACCCTGATGCGCGCCTTCGGTGGTGAACTGTCGTTCGCCAACCACAAGCAAGGTGGCGCCTTGATCACCCTGCGGCT
GCGCGCCGGCGCGCCCGGGGTCAGCCTGCAACCGTCCGAGGATCGAAGCGCATGA

Upstream 100 bases:

>100_bases
AGTCCTGTGTGAATCTTCAGCGCCGCTGCGGACCCGGCCACGGGTGCGCAGACTGCGCCGGCAAGCCTAGGCATAATGCG
CCTGTACCCACAGGCGCTCG

Downstream 100 bases:

>100_bases
CCATCGACAACCGCATCCAGGTGGTGCTGATCGACGACGATCCGCACCTGCGTCAGGCCCTCGGCCAGACCCTGGATCTG
GCCGGCCTGAAAATTCTTCC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MLMTPTLPRRPRWRSLALLALCLAPLLWPLEHLAERYYRSELAGQNRQTLDLYVANLLGTLHRYEVLPQILGDLPALRAV
LGAPDDGVTQGNANRLLKNIAAQTGAEVMYLMDTSGQTLAASNWDKHDSFVGRNFSFRPYFSEAMAGRLGRFFGLGTTSA
KRGYFFAAAVRNGEKIIGVLVIKVDLDHTESLWGKTPEQLLVTDHNGVVILTSRPEWRFRATRTLSDAERSAITAIQPYP
TREPRPLNLSPTAWLPQTQDIAETGWSVSILAPRTLIDRPVRTVVAIGGATLLVVMLLLGLMMQRRRHYLERIAFEAKAR
RELEGRVAERTSDLEGLNRRLKQEVLEREQAQQELVRAQDDLVQAGKLSALGTMSASISHELNQPLAAIRSYAENAEVLL
DHERTEDARGNLKLISELTGRMASIIAHLRAFARRDRHAPESVALQPALDDALALLAKRRRSMDVELIRDLPAATLWVEA
GETRLRQVLGNLLANALDALTEKGPPRKLWLSAESTDEGVNLYIRDNGPGFCMEALGRASEPFYTTKTRTQGLGLGLAIC
ETLMRAFGGELSFANHKQGGALITLRLRAGAPGVSLQPSEDRSA

Sequences:

>Translated_604_residues
MLMTPTLPRRPRWRSLALLALCLAPLLWPLEHLAERYYRSELAGQNRQTLDLYVANLLGTLHRYEVLPQILGDLPALRAV
LGAPDDGVTQGNANRLLKNIAAQTGAEVMYLMDTSGQTLAASNWDKHDSFVGRNFSFRPYFSEAMAGRLGRFFGLGTTSA
KRGYFFAAAVRNGEKIIGVLVIKVDLDHTESLWGKTPEQLLVTDHNGVVILTSRPEWRFRATRTLSDAERSAITAIQPYP
TREPRPLNLSPTAWLPQTQDIAETGWSVSILAPRTLIDRPVRTVVAIGGATLLVVMLLLGLMMQRRRHYLERIAFEAKAR
RELEGRVAERTSDLEGLNRRLKQEVLEREQAQQELVRAQDDLVQAGKLSALGTMSASISHELNQPLAAIRSYAENAEVLL
DHERTEDARGNLKLISELTGRMASIIAHLRAFARRDRHAPESVALQPALDDALALLAKRRRSMDVELIRDLPAATLWVEA
GETRLRQVLGNLLANALDALTEKGPPRKLWLSAESTDEGVNLYIRDNGPGFCMEALGRASEPFYTTKTRTQGLGLGLAIC
ETLMRAFGGELSFANHKQGGALITLRLRAGAPGVSLQPSEDRSA
>Mature_604_residues
MLMTPTLPRRPRWRSLALLALCLAPLLWPLEHLAERYYRSELAGQNRQTLDLYVANLLGTLHRYEVLPQILGDLPALRAV
LGAPDDGVTQGNANRLLKNIAAQTGAEVMYLMDTSGQTLAASNWDKHDSFVGRNFSFRPYFSEAMAGRLGRFFGLGTTSA
KRGYFFAAAVRNGEKIIGVLVIKVDLDHTESLWGKTPEQLLVTDHNGVVILTSRPEWRFRATRTLSDAERSAITAIQPYP
TREPRPLNLSPTAWLPQTQDIAETGWSVSILAPRTLIDRPVRTVVAIGGATLLVVMLLLGLMMQRRRHYLERIAFEAKAR
RELEGRVAERTSDLEGLNRRLKQEVLEREQAQQELVRAQDDLVQAGKLSALGTMSASISHELNQPLAAIRSYAENAEVLL
DHERTEDARGNLKLISELTGRMASIIAHLRAFARRDRHAPESVALQPALDDALALLAKRRRSMDVELIRDLPAATLWVEA
GETRLRQVLGNLLANALDALTEKGPPRKLWLSAESTDEGVNLYIRDNGPGFCMEALGRASEPFYTTKTRTQGLGLGLAIC
ETLMRAFGGELSFANHKQGGALITLRLRAGAPGVSLQPSEDRSA

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=236, Percent_Identity=28.3898305084746, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=26.1802575107296, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1790861, Length=221, Percent_Identity=34.3891402714932, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI87081816, Length=280, Percent_Identity=27.1428571428571, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788393, Length=220, Percent_Identity=31.3636363636364, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=24.0157480314961, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=266, Percent_Identity=28.1954887218045, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1789149, Length=277, Percent_Identity=26.3537906137184, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66886; Mature: 66886

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMTPTLPRRPRWRSLALLALCLAPLLWPLEHLAERYYRSELAGQNRQTLDLYVANLLGT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LHRYEVLPQILGDLPALRAVLGAPDDGVTQGNANRLLKNIAAQTGAEVMYLMDTSGQTLA
HHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEE
ASNWDKHDSFVGRNFSFRPYFSEAMAGRLGRFFGLGTTSAKRGYFFAAAVRNGEKIIGVL
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEE
VIKVDLDHTESLWGKTPEQLLVTDHNGVVILTSRPEWRFRATRTLSDAERSAITAIQPYP
EEEEECCCHHHHHCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCC
TREPRPLNLSPTAWLPQTQDIAETGWSVSILAPRTLIDRPVRTVVAIGGATLLVVMLLLG
CCCCCCCCCCCCCCCCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHH
LMMQRRRHYLERIAFEAKARRELEGRVAERTSDLEGLNRRLKQEVLEREQAQQELVRAQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVQAGKLSALGTMSASISHELNQPLAAIRSYAENAEVLLDHERTEDARGNLKLISELTG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHH
RMASIIAHLRAFARRDRHAPESVALQPALDDALALLAKRRRSMDVELIRDLPAATLWVEA
HHHHHHHHHHHHHHHCCCCCHHHEECHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEC
GETRLRQVLGNLLANALDALTEKGPPRKLWLSAESTDEGVNLYIRDNGPGFCMEALGRAS
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHCCCC
EPFYTTKTRTQGLGLGLAICETLMRAFGGELSFANHKQGGALITLRLRAGAPGVSLQPSE
CCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
DRSA
CCCC
>Mature Secondary Structure
MLMTPTLPRRPRWRSLALLALCLAPLLWPLEHLAERYYRSELAGQNRQTLDLYVANLLGT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LHRYEVLPQILGDLPALRAVLGAPDDGVTQGNANRLLKNIAAQTGAEVMYLMDTSGQTLA
HHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCEEE
ASNWDKHDSFVGRNFSFRPYFSEAMAGRLGRFFGLGTTSAKRGYFFAAAVRNGEKIIGVL
CCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEE
VIKVDLDHTESLWGKTPEQLLVTDHNGVVILTSRPEWRFRATRTLSDAERSAITAIQPYP
EEEEECCCHHHHHCCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEECCCC
TREPRPLNLSPTAWLPQTQDIAETGWSVSILAPRTLIDRPVRTVVAIGGATLLVVMLLLG
CCCCCCCCCCCCCCCCCCHHHHHCCCEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHH
LMMQRRRHYLERIAFEAKARRELEGRVAERTSDLEGLNRRLKQEVLEREQAQQELVRAQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLVQAGKLSALGTMSASISHELNQPLAAIRSYAENAEVLLDHERTEDARGNLKLISELTG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHH
RMASIIAHLRAFARRDRHAPESVALQPALDDALALLAKRRRSMDVELIRDLPAATLWVEA
HHHHHHHHHHHHHHHCCCCCHHHEECHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEC
GETRLRQVLGNLLANALDALTEKGPPRKLWLSAESTDEGVNLYIRDNGPGFCMEALGRAS
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHCCCC
EPFYTTKTRTQGLGLGLAICETLMRAFGGELSFANHKQGGALITLRLRAGAPGVSLQPSE
CCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
DRSA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]