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The map label for this gene is dctD [H]

Identifier: 77456515

GI number: 77456515

Start: 332525

End: 333913

Strand: Reverse

Name: dctD [H]

Synonym: Pfl01_0287

Alternate gene names: 77456515

Gene position: 333913-332525 (Counterclockwise)

Preceding gene: 77456516

Following gene: 77456514

Centisome position: 5.19

GC content: 65.44

Gene sequence:

>1389_bases
ATGACCATCGACAACCGCATCCAGGTGGTGCTGATCGACGACGATCCGCACCTGCGTCAGGCCCTCGGCCAGACCCTGGA
TCTGGCCGGCCTGAAAATTCTTCCGCTGTCCGAAGCCAAGGGCCTGGCCGCGCAACTGGAGCGTGACTGGCCGGGCGTCG
TGGTCAGCGACATCCGCATGCCCGGCATGGATGGCCTGGAGCTGCTGAGCGAACTGCACGCCCAGGACCCGGAACTGCCC
GTGCTGCTGATCACTGGCCACGGCGACGTGCCACTGGCCGTGCAGGCCATGCGCGCCGGTGCCTATGATTTTCTGGAAAA
ACCCTTCGCCAGCGACGCCCTGCTCGACAGCGTGCGCCGCGCCCTGGCCCTGCGGCGTCTGGTGCTGGACAACCGCAGCC
TGCGCATGGCCCTGAGCGATCGCAACGAGTTGAGCGCGCGGCTGGTCGGTCAATCCGCGCCGATGTTGCGCCTGCGCGAG
CAGATCGGCGCACTGGCGGCGACCAAGGCCGATGTCTTGATCCTCGGCGAAACCGGCGCGGGTAAAGAAGTTGTCGCCCG
CGCCCTGCACGATCTGTCGAGCCGGCGTAATGGCCCGTTCGTGGCAATCAATGCCGGTGCGCTGGCCGAGTCGGTGGTCG
AAAGCGAACTGTTCGGCCATGAACCCGGCGCCTTCACCGGCGCGCAAAAGCGCCGGATCGGCAAGTTCGAATTCGCCAAT
GGCGGCACGCTGTTCCTCGACGAAATCGAGAGCATGAGCATGGACGTGCAGGTCAAACTGCTGCGCATGCTGCAGGAGCG
CGTGGTCGAACGACTGGGCGGCAATCAGTTGATCCCGCTGGACATCCGCGTCATCGCCGCCACCAAGGAAGACCTGCGCC
AGGCCGCCGATCAGGGGCGATTCCGGGCGGACTTGTATTACCGCCTCAACGTGGCGCCGCTGCGCATTCCACCACTGCGT
GAACGGGGCGAAGACGCGTTGATGCTGTTCCAGCATTACGCCGACGAAGCCAGCGCCCGCCATGGTCTGCCAGCGCATGA
ACTGCAACCGGCCCAACGGGCGCTGCTGCTGCGCCATTCATGGCCGGGCAACGTGCGGGAATTGCAGAACGCAGCGGAGC
GTTTTGCCCTCGGCCTGGAGCTGGCGCTGGACAACACACAGCCCGATGGCAGCCCCGGCACCAGCGTCGAAATCACGGCG
GGAGGGCTCAGCGAACAAGTGGAAAACTTCGAGAAATCCCTGATCGCCGCCGAACTGGCCCGCTCTCACGGTTCCGTGCG
CAGTCTCGCCGAGGCCCTGGGCATTCCGCGCAAGACCCTGCACGACAAACTGCGCAAGCACGGCCTGAATTTCGCTGGCA
GCGGCGGCCATTCCGACGAATCCGAATGA

Upstream 100 bases:

>100_bases
TGTCGTTCGCCAACCACAAGCAAGGTGGCGCCTTGATCACCCTGCGGCTGCGCGCCGGCGCGCCCGGGGTCAGCCTGCAA
CCGTCCGAGGATCGAAGCGC

Downstream 100 bases:

>100_bases
AGCGCGCTCAATTCACTTTTCTGCCGCAAGAGGTCCGGGCATGAAGCACGACAGCCGTTATCTGGAATCCGTACTCCATC
ACGACATCCCGCTGACCCGG

Product: two component, sigma-54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTIDNRIQVVLIDDDPHLRQALGQTLDLAGLKILPLSEAKGLAAQLERDWPGVVVSDIRMPGMDGLELLSELHAQDPELP
VLLITGHGDVPLAVQAMRAGAYDFLEKPFASDALLDSVRRALALRRLVLDNRSLRMALSDRNELSARLVGQSAPMLRLRE
QIGALAATKADVLILGETGAGKEVVARALHDLSSRRNGPFVAINAGALAESVVESELFGHEPGAFTGAQKRRIGKFEFAN
GGTLFLDEIESMSMDVQVKLLRMLQERVVERLGGNQLIPLDIRVIAATKEDLRQAADQGRFRADLYYRLNVAPLRIPPLR
ERGEDALMLFQHYADEASARHGLPAHELQPAQRALLLRHSWPGNVRELQNAAERFALGLELALDNTQPDGSPGTSVEITA
GGLSEQVENFEKSLIAAELARSHGSVRSLAEALGIPRKTLHDKLRKHGLNFAGSGGHSDESE

Sequences:

>Translated_462_residues
MTIDNRIQVVLIDDDPHLRQALGQTLDLAGLKILPLSEAKGLAAQLERDWPGVVVSDIRMPGMDGLELLSELHAQDPELP
VLLITGHGDVPLAVQAMRAGAYDFLEKPFASDALLDSVRRALALRRLVLDNRSLRMALSDRNELSARLVGQSAPMLRLRE
QIGALAATKADVLILGETGAGKEVVARALHDLSSRRNGPFVAINAGALAESVVESELFGHEPGAFTGAQKRRIGKFEFAN
GGTLFLDEIESMSMDVQVKLLRMLQERVVERLGGNQLIPLDIRVIAATKEDLRQAADQGRFRADLYYRLNVAPLRIPPLR
ERGEDALMLFQHYADEASARHGLPAHELQPAQRALLLRHSWPGNVRELQNAAERFALGLELALDNTQPDGSPGTSVEITA
GGLSEQVENFEKSLIAAELARSHGSVRSLAEALGIPRKTLHDKLRKHGLNFAGSGGHSDESE
>Mature_461_residues
TIDNRIQVVLIDDDPHLRQALGQTLDLAGLKILPLSEAKGLAAQLERDWPGVVVSDIRMPGMDGLELLSELHAQDPELPV
LLITGHGDVPLAVQAMRAGAYDFLEKPFASDALLDSVRRALALRRLVLDNRSLRMALSDRNELSARLVGQSAPMLRLREQ
IGALAATKADVLILGETGAGKEVVARALHDLSSRRNGPFVAINAGALAESVVESELFGHEPGAFTGAQKRRIGKFEFANG
GTLFLDEIESMSMDVQVKLLRMLQERVVERLGGNQLIPLDIRVIAATKEDLRQAADQGRFRADLYYRLNVAPLRIPPLRE
RGEDALMLFQHYADEASARHGLPAHELQPAQRALLLRHSWPGNVRELQNAAERFALGLELALDNTQPDGSPGTSVEITAG
GLSEQVENFEKSLIAAELARSHGSVRSLAEALGIPRKTLHDKLRKHGLNFAGSGGHSDESE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=460, Percent_Identity=40.6521739130435, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1790437, Length=448, Percent_Identity=40.4017857142857, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.2478632478632, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1788905, Length=447, Percent_Identity=40.7158836689038, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=43.9252336448598, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=42.3197492163009, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=40.4692082111437, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=40.625, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1786524, Length=357, Percent_Identity=40.3361344537815, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=39.0092879256966, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=38.9776357827476, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081858, Length=285, Percent_Identity=31.5789473684211, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=37.5545851528384, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789809, Length=115, Percent_Identity=28.695652173913, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50354; Mature: 50223

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDNRIQVVLIDDDPHLRQALGQTLDLAGLKILPLSEAKGLAAQLERDWPGVVVSDIRM
CCCCCEEEEEEECCCHHHHHHHCCCCHHCCEEEEECCCCCCHHHHHHCCCCCEEEECCCC
PGMDGLELLSELHAQDPELPVLLITGHGDVPLAVQAMRAGAYDFLEKPFASDALLDSVRR
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHH
ALALRRLVLDNRSLRMALSDRNELSARLVGQSAPMLRLREQIGALAATKADVLILGETGA
HHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCC
GKEVVARALHDLSSRRNGPFVAINAGALAESVVESELFGHEPGAFTGAQKRRIGKFEFAN
CHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEECC
GGTLFLDEIESMSMDVQVKLLRMLQERVVERLGGNQLIPLDIRVIAATKEDLRQAADQGR
CCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHCCC
FRADLYYRLNVAPLRIPPLRERGEDALMLFQHYADEASARHGLPAHELQPAQRALLLRHS
EEEEEEEEEEECEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCC
WPGNVRELQNAAERFALGLELALDNTQPDGSPGTSVEITAGGLSEQVENFEKSLIAAELA
CCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RSHGSVRSLAEALGIPRKTLHDKLRKHGLNFAGSGGHSDESE
HHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIDNRIQVVLIDDDPHLRQALGQTLDLAGLKILPLSEAKGLAAQLERDWPGVVVSDIRM
CCCCEEEEEEECCCHHHHHHHCCCCHHCCEEEEECCCCCCHHHHHHCCCCCEEEECCCC
PGMDGLELLSELHAQDPELPVLLITGHGDVPLAVQAMRAGAYDFLEKPFASDALLDSVRR
CCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHH
ALALRRLVLDNRSLRMALSDRNELSARLVGQSAPMLRLREQIGALAATKADVLILGETGA
HHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCC
GKEVVARALHDLSSRRNGPFVAINAGALAESVVESELFGHEPGAFTGAQKRRIGKFEFAN
CHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEECC
GGTLFLDEIESMSMDVQVKLLRMLQERVVERLGGNQLIPLDIRVIAATKEDLRQAADQGR
CCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHCCC
FRADLYYRLNVAPLRIPPLRERGEDALMLFQHYADEASARHGLPAHELQPAQRALLLRHS
EEEEEEEEEEECEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCC
WPGNVRELQNAAERFALGLELALDNTQPDGSPGTSVEITAGGLSEQVENFEKSLIAAELA
CCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RSHGSVRSLAEALGIPRKTLHDKLRKHGLNFAGSGGHSDESE
HHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]