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The map label for this gene is cusC [H]

Identifier: 76818795

GI number: 76818795

Start: 1301176

End: 1302702

Strand: Direct

Name: cusC [H]

Synonym: BURPS1710b_A1037

Alternate gene names: 76818795

Gene position: 1301176-1302702 (Clockwise)

Preceding gene: 76818012

Following gene: 76819367

Centisome position: 40.89

GC content: 71.91

Gene sequence:

>1527_bases
ATGACCATGCGCAACGATGCGCGTCACGACGAACGGCATCAGCGGCGGCGCGGCATCGCGCGCCGCGCGGCGCGGCACGC
GGCCGCGCTCGCGCTGTGCGCGGCCGTGTCGGGCTGCCTGTCGCTCGCGCCCGACTACGCGCGCAAGCCCCCGCCCGTGC
CCGCCGCGTACCCGGCCTATCCGGGCGAACCGGGCGCCGGCTCGCGCACGCTCGAGCAGCTCGGCTGGCGCGCGTATTTC
GTCGATCCGCGCTTGCAGGCGCTGATCGAAACCGCGCTCGCGAACAACCGCGATCTGCGGATCGCCGCGCAGCGCGTCGA
GCAGGCGCGCGCGGCGTACGGCATCCGCCGCGCGGACCAATACCCGACGATCGCCGCGAACGCCGCATATCTTCGCTTTC
GCGAGCCGGGCGGCCTGCTGCTGCCGAGCCCGATCATCGGCGAGCTGTATTCGGCGTCGCTCAGCGAAGTGCAATGGGAA
ATCGATTTCTGGGGCCGCGTGCGCAGCCTGAAGGCGGCCGCGCTCGACACGTTCCTCGCGAGCGACGCGTCGCGCCGCGC
GGTGACGGTGAGCCTGATCGGCCAGGTCGCCGACGCGTATCTCGCGCTGTGCGCGTACGACGAGCGCCTCGCGCTCACCA
ACGAGACGATCGCGAGCCGCCGCGACTCGCTGCGGATCTTCCGCCGGCGCTACGAGGAAGGTGCGATCTCGAAGCTCGAT
CTGACGCAATCGGAAATCCTGCTGCAGCAGGCGCAAACGCTCGGCTCGCAGCTCCAGCAGGCGCGCGACGTGCAGCAGCA
CGCGCTCGACCTGCTCGTCGGCACGAACGCGGCCGCGCGCACGCCCGCGTCGCTCGACGACGCGAGCATCGCGCCCGATC
TCGCGCCCGGCCTGCCGTCCGAGCTGCTCGCGAACCGGCCCGACGTGGTCGCGGCCGAGTATCAGTTGCGCGCCGCACAC
GCGAACATCGGCGCGGCGCGCGCCGCGTTCTTTCCGCGCATCGCGCTCACGAGCTCGATCGGCACCGGCAGCACCGAGCT
GCACAACCTGTTCGCCTCCGGCACGGCCGCGTGGAACTTCATTCCGAACCTGTCGGTGCCGATCTTCGACGCGGGCCGCA
ACATCGGCAACCTGAAGCTCGCGAACGCGCGCCGCGACGAAGCGCTCGCCCAGTATGAGAAGACGATCCAGACGGCGTTT
CGCGACGTCGCCGACGCGCTCGCCGCGCGCCACTGGCTCGCCGATCAGGTGCGCATCGCGCAGGAAACGCTCGCGACGCA
AGCCGAGCGCGCGCGGCTCGCGAAGCTGCGCTACGACAGCGGCGCCGCGCGCTTTCTCGAAGTGCTCGACGCGCAGCGCG
ACTTGCTGAACGCGCAGCAGCAGCTCGTGCAGACGCGCCGCGCGCTGCTCGCGAGCCGCGTCGCGCTGTACGCGGCGCTC
GGCGGGCGTCTGGACGAGCCCGCGCGCGACGGCGCCGCGCCGCGCCCCTCTCCCGAACCCTCAGCGAAACAAGGTCCGTT
GTCATGA

Upstream 100 bases:

>100_bases
AGAGCGGCGCGCGGCGCTTCTCCGCGCCGCGCGACGGTGAGCGTTTTCGGGCGCGCGCGGCTTTCACGAAGCACTGCCGG
CTCCAACGAACACCGAGGAC

Downstream 100 bases:

>100_bases
TCCAAGTCACCAGGAAGCAGATCGTCGCCGCGGCGGCCGTCGTCGTGGTCGCGCTCGGCGCTTACTACGGCTGGACGCTG
CTGCGCCACCAGGGCCCCGG

Product: RND efflux system, outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTMRNDARHDERHQRRRGIARRAARHAAALALCAAVSGCLSLAPDYARKPPPVPAAYPAYPGEPGAGSRTLEQLGWRAYF
VDPRLQALIETALANNRDLRIAAQRVEQARAAYGIRRADQYPTIAANAAYLRFREPGGLLLPSPIIGELYSASLSEVQWE
IDFWGRVRSLKAAALDTFLASDASRRAVTVSLIGQVADAYLALCAYDERLALTNETIASRRDSLRIFRRRYEEGAISKLD
LTQSEILLQQAQTLGSQLQQARDVQQHALDLLVGTNAAARTPASLDDASIAPDLAPGLPSELLANRPDVVAAEYQLRAAH
ANIGAARAAFFPRIALTSSIGTGSTELHNLFASGTAAWNFIPNLSVPIFDAGRNIGNLKLANARRDEALAQYEKTIQTAF
RDVADALAARHWLADQVRIAQETLATQAERARLAKLRYDSGAARFLEVLDAQRDLLNAQQQLVQTRRALLASRVALYAAL
GGRLDEPARDGAAPRPSPEPSAKQGPLS

Sequences:

>Translated_508_residues
MTMRNDARHDERHQRRRGIARRAARHAAALALCAAVSGCLSLAPDYARKPPPVPAAYPAYPGEPGAGSRTLEQLGWRAYF
VDPRLQALIETALANNRDLRIAAQRVEQARAAYGIRRADQYPTIAANAAYLRFREPGGLLLPSPIIGELYSASLSEVQWE
IDFWGRVRSLKAAALDTFLASDASRRAVTVSLIGQVADAYLALCAYDERLALTNETIASRRDSLRIFRRRYEEGAISKLD
LTQSEILLQQAQTLGSQLQQARDVQQHALDLLVGTNAAARTPASLDDASIAPDLAPGLPSELLANRPDVVAAEYQLRAAH
ANIGAARAAFFPRIALTSSIGTGSTELHNLFASGTAAWNFIPNLSVPIFDAGRNIGNLKLANARRDEALAQYEKTIQTAF
RDVADALAARHWLADQVRIAQETLATQAERARLAKLRYDSGAARFLEVLDAQRDLLNAQQQLVQTRRALLASRVALYAAL
GGRLDEPARDGAAPRPSPEPSAKQGPLS
>Mature_507_residues
TMRNDARHDERHQRRRGIARRAARHAAALALCAAVSGCLSLAPDYARKPPPVPAAYPAYPGEPGAGSRTLEQLGWRAYFV
DPRLQALIETALANNRDLRIAAQRVEQARAAYGIRRADQYPTIAANAAYLRFREPGGLLLPSPIIGELYSASLSEVQWEI
DFWGRVRSLKAAALDTFLASDASRRAVTVSLIGQVADAYLALCAYDERLALTNETIASRRDSLRIFRRRYEEGAISKLDL
TQSEILLQQAQTLGSQLQQARDVQQHALDLLVGTNAAARTPASLDDASIAPDLAPGLPSELLANRPDVVAAEYQLRAAHA
NIGAARAAFFPRIALTSSIGTGSTELHNLFASGTAAWNFIPNLSVPIFDAGRNIGNLKLANARRDEALAQYEKTIQTAFR
DVADALAARHWLADQVRIAQETLATQAERARLAKLRYDSGAARFLEVLDAQRDLLNAQQQLVQTRRALLASRVALYAALG
GRLDEPARDGAAPRPSPEPSAKQGPLS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=456, Percent_Identity=36.1842105263158, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI2367348, Length=489, Percent_Identity=26.1758691206544, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55254; Mature: 55123

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMRNDARHDERHQRRRGIARRAARHAAALALCAAVSGCLSLAPDYARKPPPVPAAYPAY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCC
PGEPGAGSRTLEQLGWRAYFVDPRLQALIETALANNRDLRIAAQRVEQARAAYGIRRADQ
CCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
YPTIAANAAYLRFREPGGLLLPSPIIGELYSASLSEVQWEIDFWGRVRSLKAAALDTFLA
CCCEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
SDASRRAVTVSLIGQVADAYLALCAYDERLALTNETIASRRDSLRIFRRRYEEGAISKLD
CCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LTQSEILLQQAQTLGSQLQQARDVQQHALDLLVGTNAAARTPASLDDASIAPDLAPGLPS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCH
ELLANRPDVVAAEYQLRAAHANIGAARAAFFPRIALTSSIGTGSTELHNLFASGTAAWNF
HHHCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHEEE
IPNLSVPIFDAGRNIGNLKLANARRDEALAQYEKTIQTAFRDVADALAARHWLADQVRIA
CCCCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QETLATQAERARLAKLRYDSGAARFLEVLDAQRDLLNAQQQLVQTRRALLASRVALYAAL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGRLDEPARDGAAPRPSPEPSAKQGPLS
CCCCCCCHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMRNDARHDERHQRRRGIARRAARHAAALALCAAVSGCLSLAPDYARKPPPVPAAYPAY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCC
PGEPGAGSRTLEQLGWRAYFVDPRLQALIETALANNRDLRIAAQRVEQARAAYGIRRADQ
CCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
YPTIAANAAYLRFREPGGLLLPSPIIGELYSASLSEVQWEIDFWGRVRSLKAAALDTFLA
CCCEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHH
SDASRRAVTVSLIGQVADAYLALCAYDERLALTNETIASRRDSLRIFRRRYEEGAISKLD
CCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LTQSEILLQQAQTLGSQLQQARDVQQHALDLLVGTNAAARTPASLDDASIAPDLAPGLPS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCH
ELLANRPDVVAAEYQLRAAHANIGAARAAFFPRIALTSSIGTGSTELHNLFASGTAAWNF
HHHCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHEEE
IPNLSVPIFDAGRNIGNLKLANARRDEALAQYEKTIQTAFRDVADALAARHWLADQVRIA
CCCCCCEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QETLATQAERARLAKLRYDSGAARFLEVLDAQRDLLNAQQQLVQTRRALLASRVALYAAL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGRLDEPARDGAAPRPSPEPSAKQGPLS
CCCCCCCHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]