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The map label for this gene is proP7 [H]

Identifier: 76818468

GI number: 76818468

Start: 315069

End: 316349

Strand: Direct

Name: proP7 [H]

Synonym: BURPS1710b_A0209

Alternate gene names: 76818468

Gene position: 315069-316349 (Clockwise)

Preceding gene: 76817776

Following gene: 76818927

Centisome position: 9.9

GC content: 45.28

Gene sequence:

>1281_bases
GTGCGATCAAAATCTGGACAAACTCTAAAGATTGCGCTGACTGCAAATCTTTTCGAGTGGTATGAGTTCTCACTCACGGC
GTTCATGGCGCTAGAGATAGGTAGGCTTTTCTTCCCCGATACAAACGAGAAAACGGCCTTAATTTCAAGCTTCTCAGTGT
TCGCCAGCAGCTATCTTGCGCGACCGATCGGCAGCATACTTTTTGGCATTTTAGGAGCTCGATACGGAACAGGGGCTTCG
TTAAAAATCAGCATGATCGGAATGGCTGTGCCCGCCTCGCTAATTGCAGTTCTCCCTACATATCAAACAGCCGGCCATTT
CTCAACTTTCTTGTTGCTAGCTCTGAAAATAACTCAGGGCTTCTGCGCAGGAGGAGAAATGCCATTAAGCGGATATTTCG
TATCGCTCAATGCAGACAGGACGAATCGCGGCCTCTATTGCGCCTTAACAGTAGCGAGCGGATTTATCGGCATGCTATTA
GCTTCCGCGGCTGTATTTTTGCTTCCGCATATAAGATATGCCCTCGACCACCTAGCTAGCCCTGAAAATTACGCCTACCT
GAACGAGCCGTGGCGCTGGCCGTTTTTTCTTTGCATGCCATTATCTATTTCAATATATTTATTACGATCCGCAATAAACA
AGAGTGAAGTGAAGGTAGATTCCGGAAAATTGAGGGCAAAGCCCACTTTTCCGCTACTTCAGTCGCTCGCCGTCGTCGCA
TTTGCAGAAATAGTTATATATATAATTTTTGTATGGATGCCAAATTATTTGCACGTCTACCTTGGGAAGCAAAGTTTTGA
CGCGAGACTTTCAAATATTTCATCCCTGATAATATTCTCGATATCGATGGCTTGTGCCGGCTATGCTGAGCGTTATTTTT
GCGCCAGAAATATCGCACTATTCGGAACCATAGCGACGGCAATCTCCGTCTATCCATCATTTATTATAATGCAATACGGG
GGCATATTCGCACTATTGTTTTCCCAGGCCATCCTCGCAATATCGGCTGGATGTGTCGTTGGAGTAATTTTCGTCGTGTT
GCCTGATCTATTCAAGAAAAATTGGGGGAGCTTGGGAATGGTCGTCACCTACTCATTTGCCACCGCTATTTTTGGTGGAA
CCGCCCCTGTTTTCTGCGCCTACCTGATCGAATTAACTCATTTTATTGCCGCACCTGCCATTTATATATTCTTAATGGGA
ATGTTGGCTGCAGCCGCCATCTACAAAATTTATCCACCAAGTAAAACGAAATTTAGATATCGAGCACCGAATTCCGTTTG
A

Upstream 100 bases:

>100_bases
TCTCAGATAGAACGGTTGAATGTCATTGCGCAGCTCTGCGCAGCAAGATAATTGATGGAAACCTGCCATATATCCTGTCG
CTTGTAAAAGGAAGCTTCTC

Downstream 100 bases:

>100_bases
TTGAAAGCATCATTCATCGTCACTTAAAAATTATTGTGAATAAATCGGCTAATTTGCCATCGGAGTATCTGATCACGCCG
GAACCTCCGGGTGACGAGGC

Product: putative sugar transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MRSKSGQTLKIALTANLFEWYEFSLTAFMALEIGRLFFPDTNEKTALISSFSVFASSYLARPIGSILFGILGARYGTGAS
LKISMIGMAVPASLIAVLPTYQTAGHFSTFLLLALKITQGFCAGGEMPLSGYFVSLNADRTNRGLYCALTVASGFIGMLL
ASAAVFLLPHIRYALDHLASPENYAYLNEPWRWPFFLCMPLSISIYLLRSAINKSEVKVDSGKLRAKPTFPLLQSLAVVA
FAEIVIYIIFVWMPNYLHVYLGKQSFDARLSNISSLIIFSISMACAGYAERYFCARNIALFGTIATAISVYPSFIIMQYG
GIFALLFSQAILAISAGCVVGVIFVVLPDLFKKNWGSLGMVVTYSFATAIFGGTAPVFCAYLIELTHFIAAPAIYIFLMG
MLAAAAIYKIYPPSKTKFRYRAPNSV

Sequences:

>Translated_426_residues
MRSKSGQTLKIALTANLFEWYEFSLTAFMALEIGRLFFPDTNEKTALISSFSVFASSYLARPIGSILFGILGARYGTGAS
LKISMIGMAVPASLIAVLPTYQTAGHFSTFLLLALKITQGFCAGGEMPLSGYFVSLNADRTNRGLYCALTVASGFIGMLL
ASAAVFLLPHIRYALDHLASPENYAYLNEPWRWPFFLCMPLSISIYLLRSAINKSEVKVDSGKLRAKPTFPLLQSLAVVA
FAEIVIYIIFVWMPNYLHVYLGKQSFDARLSNISSLIIFSISMACAGYAERYFCARNIALFGTIATAISVYPSFIIMQYG
GIFALLFSQAILAISAGCVVGVIFVVLPDLFKKNWGSLGMVVTYSFATAIFGGTAPVFCAYLIELTHFIAAPAIYIFLMG
MLAAAAIYKIYPPSKTKFRYRAPNSV
>Mature_426_residues
MRSKSGQTLKIALTANLFEWYEFSLTAFMALEIGRLFFPDTNEKTALISSFSVFASSYLARPIGSILFGILGARYGTGAS
LKISMIGMAVPASLIAVLPTYQTAGHFSTFLLLALKITQGFCAGGEMPLSGYFVSLNADRTNRGLYCALTVASGFIGMLL
ASAAVFLLPHIRYALDHLASPENYAYLNEPWRWPFFLCMPLSISIYLLRSAINKSEVKVDSGKLRAKPTFPLLQSLAVVA
FAEIVIYIIFVWMPNYLHVYLGKQSFDARLSNISSLIIFSISMACAGYAERYFCARNIALFGTIATAISVYPSFIIMQYG
GIFALLFSQAILAISAGCVVGVIFVVLPDLFKKNWGSLGMVVTYSFATAIFGGTAPVFCAYLIELTHFIAAPAIYIFLMG
MLAAAAIYKIYPPSKTKFRYRAPNSV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=419, Percent_Identity=25.2983293556086, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1788292, Length=384, Percent_Identity=25.2604166666667, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788942, Length=382, Percent_Identity=27.4869109947644, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789941, Length=216, Percent_Identity=30.0925925925926, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46622; Mature: 46622

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKSGQTLKIALTANLFEWYEFSLTAFMALEIGRLFFPDTNEKTALISSFSVFASSYLA
CCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RPIGSILFGILGARYGTGASLKISMIGMAVPASLIAVLPTYQTAGHFSTFLLLALKITQG
HHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
FCAGGEMPLSGYFVSLNADRTNRGLYCALTVASGFIGMLLASAAVFLLPHIRYALDHLAS
CCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PENYAYLNEPWRWPFFLCMPLSISIYLLRSAINKSEVKVDSGKLRAKPTFPLLQSLAVVA
CCCCEECCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEECCCCEECCCCHHHHHHHHHHH
FAEIVIYIIFVWMPNYLHVYLGKQSFDARLSNISSLIIFSISMACAGYAERYFCARNIAL
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGTIATAISVYPSFIIMQYGGIFALLFSQAILAISAGCVVGVIFVVLPDLFKKNWGSLGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
VVTYSFATAIFGGTAPVFCAYLIELTHFIAAPAIYIFLMGMLAAAAIYKIYPPSKTKFRY
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEE
RAPNSV
CCCCCC
>Mature Secondary Structure
MRSKSGQTLKIALTANLFEWYEFSLTAFMALEIGRLFFPDTNEKTALISSFSVFASSYLA
CCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
RPIGSILFGILGARYGTGASLKISMIGMAVPASLIAVLPTYQTAGHFSTFLLLALKITQG
HHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
FCAGGEMPLSGYFVSLNADRTNRGLYCALTVASGFIGMLLASAAVFLLPHIRYALDHLAS
CCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PENYAYLNEPWRWPFFLCMPLSISIYLLRSAINKSEVKVDSGKLRAKPTFPLLQSLAVVA
CCCCEECCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEECCCCEECCCCHHHHHHHHHHH
FAEIVIYIIFVWMPNYLHVYLGKQSFDARLSNISSLIIFSISMACAGYAERYFCARNIAL
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGTIATAISVYPSFIIMQYGGIFALLFSQAILAISAGCVVGVIFVVLPDLFKKNWGSLGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
VVTYSFATAIFGGTAPVFCAYLIELTHFIAAPAIYIFLMGMLAAAAIYKIYPPSKTKFRY
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEE
RAPNSV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]