Click here to switch to the map view.

The map label for this gene is thiE2 [C]

Identifier: 76818927

GI number: 76818927

Start: 316346

End: 316984

Strand: Direct

Name: thiE2 [C]

Synonym: BURPS1710b_A0210

Alternate gene names: 76818927

Gene position: 316346-316984 (Clockwise)

Preceding gene: 76818468

Following gene: 76818508

Centisome position: 9.94

GC content: 55.24

Gene sequence:

>639_bases
TTGATTGAAAGCATCATTCATCGTCACTTAAAAATTATTGTGAATAAATCGGCTAATTTGCCATCGGAGTATCTGATCAC
GCCGGAACCTCCGGGTGACGAGGCGCTGAGCGACTACCTCGCGACCCTGGAGCGAACATTGAAAGCCGGCATCAGTCTTG
TACAGCTACGCGCGAAAGCTGTAACTGCGCCGTATTACGCACGTCTGACGGAATATGCCTTGGCTTGTTGCCGAAGGTAC
AATGCGCGACTGCTCGTGAATGCCGCACCTGAAGTGGCGCGGGGCTTGCATACAGATGGCGTCCATCTCACCAGCACTCG
TCTGATGACATGCTCGACTAGACCGCTGCCAGCCGGATTGCTGGTTTCGGCCGCATGCCACGATGAGGATCAGGTGAGAC
ATGCAGATTCAATAGGGGTCGATCTGATCACCATCTCGCCGGTTATGCCGACTGCGACTCATACGACTGCAGAGCCACTG
GGTTGGCCACGTTTTCGAGAGCTTGCAACGCTGACATCGGTTCCGGTCTATGCGTTAGGAGGGATGTCCGTCGACAGCTT
GGCTGAAGCTCGCAACGCGGGCGCGTACGGTATTGCCGCGATTCGTGCCTTTTGGGGAAGCAACGTCGATCGATCGTAG

Upstream 100 bases:

>100_bases
CCATTTATATATTCTTAATGGGAATGTTGGCTGCAGCCGCCATCTACAAAATTTATCCACCAAGTAAAACGAAATTTAGA
TATCGAGCACCGAATTCCGT

Downstream 100 bases:

>100_bases
CTGCGAGAGGATATTTGCCAGTTTTTCACAAGCGAGCGAATTGAGTTCGACGAAAACGCATGGTGAAATATGATGATGTA
CCGCTGGAGCATCGGTCTCG

Product: dGTP-pyrophosphohydrolase; thiamine phosphate synthase

Products: diphosphate; thiamine monophosphate

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIESIIHRHLKIIVNKSANLPSEYLITPEPPGDEALSDYLATLERTLKAGISLVQLRAKAVTAPYYARLTEYALACCRRY
NARLLVNAAPEVARGLHTDGVHLTSTRLMTCSTRPLPAGLLVSAACHDEDQVRHADSIGVDLITISPVMPTATHTTAEPL
GWPRFRELATLTSVPVYALGGMSVDSLAEARNAGAYGIAAIRAFWGSNVDRS

Sequences:

>Translated_212_residues
MIESIIHRHLKIIVNKSANLPSEYLITPEPPGDEALSDYLATLERTLKAGISLVQLRAKAVTAPYYARLTEYALACCRRY
NARLLVNAAPEVARGLHTDGVHLTSTRLMTCSTRPLPAGLLVSAACHDEDQVRHADSIGVDLITISPVMPTATHTTAEPL
GWPRFRELATLTSVPVYALGGMSVDSLAEARNAGAYGIAAIRAFWGSNVDRS
>Mature_212_residues
MIESIIHRHLKIIVNKSANLPSEYLITPEPPGDEALSDYLATLERTLKAGISLVQLRAKAVTAPYYARLTEYALACCRRY
NARLLVNAAPEVARGLHTDGVHLTSTRLMTCSTRPLPAGLLVSAACHDEDQVRHADSIGVDLITISPVMPTATHTTAEPL
GWPRFRELATLTSVPVYALGGMSVDSLAEARNAGAYGIAAIRAFWGSNVDRS

Specific function: Condenses 4-Methyl-5-(Beta-Hydroxyethyl)-Thiazole Monophosphate (Thz-P) And 4-Amino-5-Hydroxymethyl Pyrimidine Pyrophosphate (Hmp-Pp) To Form Thiamine Monophosphate (TMP). [C]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3

Molecular weight: Translated: 22905; Mature: 22905

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIESIIHRHLKIIVNKSANLPSEYLITPEPPGDEALSDYLATLERTLKAGISLVQLRAKA
CHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTAPYYARLTEYALACCRRYNARLLVNAAPEVARGLHTDGVHLTSTRLMTCSTRPLPAGL
HCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCEEEECEEEEEECCCCCCHHH
LVSAACHDEDQVRHADSIGVDLITISPVMPTATHTTAEPLGWPRFRELATLTSVPVYALG
EEHHHCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHEEC
GMSVDSLAEARNAGAYGIAAIRAFWGSNVDRS
CCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIESIIHRHLKIIVNKSANLPSEYLITPEPPGDEALSDYLATLERTLKAGISLVQLRAKA
CHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTAPYYARLTEYALACCRRYNARLLVNAAPEVARGLHTDGVHLTSTRLMTCSTRPLPAGL
HCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCEEEECEEEEEECCCCCCHHH
LVSAACHDEDQVRHADSIGVDLITISPVMPTATHTTAEPLGWPRFRELATLTSVPVYALG
EEHHHCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHEEC
GMSVDSLAEARNAGAYGIAAIRAFWGSNVDRS
CCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0004 {thiazole} 0.00085 {hydroxymethylpyrimidine} [C]

Substrates: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphono-oxyethyl)-thiazole

Specific reaction: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine monophosphate

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]