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The map label for this gene is mrcA [H]

Identifier: 76817409

GI number: 76817409

Start: 2161573

End: 2163714

Strand: Direct

Name: mrcA [H]

Synonym: BURPS1710b_A1773

Alternate gene names: 76817409

Gene position: 2161573-2163714 (Clockwise)

Preceding gene: 76817823

Following gene: 76818959

Centisome position: 67.94

GC content: 72.92

Gene sequence:

>2142_bases
ATGACCGATCACACGCCGCACTCGTCGCCGCCCGCCTCGCCGACGCCGCCTCAGGGGCCCGCGCCGAAGCGCCGCCGCTA
TTTCCGCACGCTCGCGGGCCTCGTGCTCGGCGCGGCGATCGCGGGCGGCGCGGTCGGCACGTGGACGCTGCATCGCATCT
GGACGGCGCTGCCGTCTGTCGAGCCGCTCGCCGTCTATCGTCCGGCGCTGCCGCTGCGGATCTTTTCGCGCGAAGGCGAG
CTGCTCGCCGAATACGGCGTCGAGCGCCGCGAGTTCGTGCCGCTCGACCGCATCCCGCCGCTCGTGCGGCACGCGCTGCT
CGCGGCCGAGGATGCGCAGTTCTACACGCACGGCGCGGTCGACATCGGCGGGCTCGCGCGCGCGACGGTGGCGAACGTCG
TCACCGGCCAGCCCGGGCAGGGCGGCAGCACGATCACGATGCAGGTCGCGCGCAATTTCTTTCTGACGCGCGAGAAGGTG
CTGAGCCGCAAGCTCGCCGAGATCCTGATGTCGTACAAGCTCGAACGCGCGTACAGCAAGGACAAGCTGCTCGAGCTGTA
CATGAACGAGATCTATCTCGGCGAGCGCGCGTACGGGTTCGCGGCGGCCGCGTCGGTGTACTTCGGCAAGCCGCTCGATG
CGCTCACGCCCGGCGAGGCGGCGGTGCTCGCGGGCCTGCCGAAGGCGCCGTCCGCGTTCAATCCGGTCGTCAATCCGGCG
CGGGCGACGATGCGCCGCAACTACGTGCTCGGCCGCATGCGCGCGCTCGGGTATCTGGACGAGGCCGCGTACCGGCAGGC
CGCCGACGCGCCGATCGCGCTCGCGACCACGCCGCCGCCCGGCATCCTCGCCGCGCCGTACGTCGCCGAGCGCGCGCGCC
GGATGATGGTCGAGCGCTTCCATGACGACGCCTACACGCTCGGCCTGGACGTGACGACGACGATCGCGATGCGCGAGCAG
CGCGCGGCCGACGCGGCGCTCGCGCGCGGGCTCGCGCAGGTCGGCCGGCGCGACGCGAAGGACGCGCTCGAAGGCGCGCT
CGTGTCGATCGACGCGGCGACGGGCGACATTCTCGCGCTCGTCGGCGGCGCGGACTTCTCGCACAACGTGTTCGATCATG
CGTTGCAGGCGTATCGGCAACCGGGTTCGAGCTTCAAGCCGTTCGTCTATTCGGCCGCGCTGGAGAAAGGGATGTTCCCC
GGCGTGCTGATCGACGACACGCAGCGCACGCTGTCGCGCGACGAAACCGGCGCGAACCCGTGGCGGCCGCGCAATTTCGG
CAACCACTACGAAGGCTTCATTCCGGTGCGGCGCGGGCTGATGCGCTCGAAGAACCTCGTCGCGGTGAGCCTGATGCAGG
TCGCGCGGCCGGATTTCGTCCAGCAGCACGCGGCGCGCTTCGGCTTCGAGCCGCTGCGCAACCCCGCGTCGCTGCCGCTC
GCGCTCGGCGCGGGCTCGGCGACGCCGCTGGAGGTGGCGAGCGCCTACGGCGTGTTCGCCAACGGCGGCGTGCGCATGGA
GCCGCGCCTCATCGAATCGGTGCGGCAGCGGCACGGCGGCGCGCTGTTCGACGCGCAGCCCGCGCCCGGCGTGCGCGTCA
TCTCGGCGCGCAACGCGTTCGTGATGGACAGCATGCTGCGCGACGTCGTCGCGCACGGCACCGCGCGCCGCGCGGTCGCG
CTCAAGCGCGCGGACGCGGCGGGCAAGACCGGCACGTCGAACGGCTCGAAGGACGTGTGGTTCGCCGGCTACTCGTCGGG
CGTCGTATCGGTCGTCTGGATGGGCTACGACGCGCCGCGCAGCCTCGGCCGCGCGACGGGCTCGTCGGCCGCGCTGCCCG
TGTGGGTCGACTACATGAAGACCGCGGTCGACGGCCGCGGCGCGATCGAGCGCACGCCGCCCGCGGACGTCGCGCTCGTC
GACGGCGATTTCGTCTATGCCGAATACGCGAACGGCGACAAGTGCGCGCCGTCGCTGCCGCCGTTCGTGCGCAGCCCGTT
CGCGTGCGGCGCGGCGCGCGGTGCGCCGGCGGACGGCGGCGCGCCGGCGAGCGCGGCAGGGGCGGCGGGTGCGCCGACGC
CGGCCGCCGTCGACGCGGCCGAGCGCGCGCGCGTGCTCGAGCTGTTTCGCACCGAAGACTGA

Upstream 100 bases:

>100_bases
TCGGTATCGGGATCCTCCCCGGACAATGCGCGCGTGCGCATCGTGCACTGCAACATCCGCGCGAGGGCGTACGCCGCATT
TGCCAACGAAGGATTTCGAC

Downstream 100 bases:

>100_bases
GCGGGGACGGCCATGCATACGCTCTACCTGATCGCGATCGTCGCCGAGGCGATGTCGGGCGCGCTGATGGGCATGCAGCG
CGGGATGGACCGGTTCGGCC

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 713; Mature: 712

Protein sequence:

>713_residues
MTDHTPHSSPPASPTPPQGPAPKRRRYFRTLAGLVLGAAIAGGAVGTWTLHRIWTALPSVEPLAVYRPALPLRIFSREGE
LLAEYGVERREFVPLDRIPPLVRHALLAAEDAQFYTHGAVDIGGLARATVANVVTGQPGQGGSTITMQVARNFFLTREKV
LSRKLAEILMSYKLERAYSKDKLLELYMNEIYLGERAYGFAAAASVYFGKPLDALTPGEAAVLAGLPKAPSAFNPVVNPA
RATMRRNYVLGRMRALGYLDEAAYRQAADAPIALATTPPPGILAAPYVAERARRMMVERFHDDAYTLGLDVTTTIAMREQ
RAADAALARGLAQVGRRDAKDALEGALVSIDAATGDILALVGGADFSHNVFDHALQAYRQPGSSFKPFVYSAALEKGMFP
GVLIDDTQRTLSRDETGANPWRPRNFGNHYEGFIPVRRGLMRSKNLVAVSLMQVARPDFVQQHAARFGFEPLRNPASLPL
ALGAGSATPLEVASAYGVFANGGVRMEPRLIESVRQRHGGALFDAQPAPGVRVISARNAFVMDSMLRDVVAHGTARRAVA
LKRADAAGKTGTSNGSKDVWFAGYSSGVVSVVWMGYDAPRSLGRATGSSAALPVWVDYMKTAVDGRGAIERTPPADVALV
DGDFVYAEYANGDKCAPSLPPFVRSPFACGAARGAPADGGAPASAAGAAGAPTPAAVDAAERARVLELFRTED

Sequences:

>Translated_713_residues
MTDHTPHSSPPASPTPPQGPAPKRRRYFRTLAGLVLGAAIAGGAVGTWTLHRIWTALPSVEPLAVYRPALPLRIFSREGE
LLAEYGVERREFVPLDRIPPLVRHALLAAEDAQFYTHGAVDIGGLARATVANVVTGQPGQGGSTITMQVARNFFLTREKV
LSRKLAEILMSYKLERAYSKDKLLELYMNEIYLGERAYGFAAAASVYFGKPLDALTPGEAAVLAGLPKAPSAFNPVVNPA
RATMRRNYVLGRMRALGYLDEAAYRQAADAPIALATTPPPGILAAPYVAERARRMMVERFHDDAYTLGLDVTTTIAMREQ
RAADAALARGLAQVGRRDAKDALEGALVSIDAATGDILALVGGADFSHNVFDHALQAYRQPGSSFKPFVYSAALEKGMFP
GVLIDDTQRTLSRDETGANPWRPRNFGNHYEGFIPVRRGLMRSKNLVAVSLMQVARPDFVQQHAARFGFEPLRNPASLPL
ALGAGSATPLEVASAYGVFANGGVRMEPRLIESVRQRHGGALFDAQPAPGVRVISARNAFVMDSMLRDVVAHGTARRAVA
LKRADAAGKTGTSNGSKDVWFAGYSSGVVSVVWMGYDAPRSLGRATGSSAALPVWVDYMKTAVDGRGAIERTPPADVALV
DGDFVYAEYANGDKCAPSLPPFVRSPFACGAARGAPADGGAPASAAGAAGAPTPAAVDAAERARVLELFRTED
>Mature_712_residues
TDHTPHSSPPASPTPPQGPAPKRRRYFRTLAGLVLGAAIAGGAVGTWTLHRIWTALPSVEPLAVYRPALPLRIFSREGEL
LAEYGVERREFVPLDRIPPLVRHALLAAEDAQFYTHGAVDIGGLARATVANVVTGQPGQGGSTITMQVARNFFLTREKVL
SRKLAEILMSYKLERAYSKDKLLELYMNEIYLGERAYGFAAAASVYFGKPLDALTPGEAAVLAGLPKAPSAFNPVVNPAR
ATMRRNYVLGRMRALGYLDEAAYRQAADAPIALATTPPPGILAAPYVAERARRMMVERFHDDAYTLGLDVTTTIAMREQR
AADAALARGLAQVGRRDAKDALEGALVSIDAATGDILALVGGADFSHNVFDHALQAYRQPGSSFKPFVYSAALEKGMFPG
VLIDDTQRTLSRDETGANPWRPRNFGNHYEGFIPVRRGLMRSKNLVAVSLMQVARPDFVQQHAARFGFEPLRNPASLPLA
LGAGSATPLEVASAYGVFANGGVRMEPRLIESVRQRHGGALFDAQPAPGVRVISARNAFVMDSMLRDVVAHGTARRAVAL
KRADAAGKTGTSNGSKDVWFAGYSSGVVSVVWMGYDAPRSLGRATGSSAALPVWVDYMKTAVDGRGAIERTPPADVALVD
GDFVYAEYANGDKCAPSLPPFVRSPFACGAARGAPADGGAPASAAGAAGAPTPAAVDAAERARVLELFRTED

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=306, Percent_Identity=44.4444444444444, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1786343, Length=576, Percent_Identity=31.7708333333333, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1788867, Length=523, Percent_Identity=31.5487571701721, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789601, Length=171, Percent_Identity=32.7485380116959, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 76014; Mature: 75883

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHTPHSSPPASPTPPQGPAPKRRRYFRTLAGLVLGAAIAGGAVGTWTLHRIWTALPSV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
EPLAVYRPALPLRIFSREGELLAEYGVERREFVPLDRIPPLVRHALLAAEDAQFYTHGAV
CCHHHHCCCCCEEEECCCCHHHHHHCCCHHHCCCHHCCCHHHHHHHHHHCCCCCEECCCC
DIGGLARATVANVVTGQPGQGGSTITMQVARNFFLTREKVLSRKLAEILMSYKLERAYSK
CCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
DKLLELYMNEIYLGERAYGFAAAASVYFGKPLDALTPGEAAVLAGLPKAPSAFNPVVNPA
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHH
RATMRRNYVLGRMRALGYLDEAAYRQAADAPIALATTPPPGILAAPYVAERARRMMVERF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCHHHHHHHHHHHHHH
HDDAYTLGLDVTTTIAMREQRAADAALARGLAQVGRRDAKDALEGALVSIDAATGDILAL
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCCEEEE
VGGADFSHNVFDHALQAYRQPGSSFKPFVYSAALEKGMFPGVLIDDTQRTLSRDETGANP
ECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCC
WRPRNFGNHYEGFIPVRRGLMRSKNLVAVSLMQVARPDFVQQHAARFGFEPLRNPASLPL
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCHHCCCCCCCCE
ALGAGSATPLEVASAYGVFANGGVRMEPRLIESVRQRHGGALFDAQPAPGVRVISARNAF
EECCCCCCHHHHHHHCCEEECCCEEECHHHHHHHHHHCCCCEEECCCCCCEEEEECCCHH
VMDSMLRDVVAHGTARRAVALKRADAAGKTGTSNGSKDVWFAGYSSGVVSVVWMGYDAPR
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCHH
SLGRATGSSAALPVWVDYMKTAVDGRGAIERTPPADVALVDGDFVYAEYANGDKCAPSLP
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCC
PFVRSPFACGAARGAPADGGAPASAAGAAGAPTPAAVDAAERARVLELFRTED
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDHTPHSSPPASPTPPQGPAPKRRRYFRTLAGLVLGAAIAGGAVGTWTLHRIWTALPSV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
EPLAVYRPALPLRIFSREGELLAEYGVERREFVPLDRIPPLVRHALLAAEDAQFYTHGAV
CCHHHHCCCCCEEEECCCCHHHHHHCCCHHHCCCHHCCCHHHHHHHHHHCCCCCEECCCC
DIGGLARATVANVVTGQPGQGGSTITMQVARNFFLTREKVLSRKLAEILMSYKLERAYSK
CCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
DKLLELYMNEIYLGERAYGFAAAASVYFGKPLDALTPGEAAVLAGLPKAPSAFNPVVNPA
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHH
RATMRRNYVLGRMRALGYLDEAAYRQAADAPIALATTPPPGILAAPYVAERARRMMVERF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEECCHHHHHHHHHHHHHH
HDDAYTLGLDVTTTIAMREQRAADAALARGLAQVGRRDAKDALEGALVSIDAATGDILAL
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCCEEEE
VGGADFSHNVFDHALQAYRQPGSSFKPFVYSAALEKGMFPGVLIDDTQRTLSRDETGANP
ECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCC
WRPRNFGNHYEGFIPVRRGLMRSKNLVAVSLMQVARPDFVQQHAARFGFEPLRNPASLPL
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCHHCCCCCCCCE
ALGAGSATPLEVASAYGVFANGGVRMEPRLIESVRQRHGGALFDAQPAPGVRVISARNAF
EECCCCCCHHHHHHHCCEEECCCEEECHHHHHHHHHHCCCCEEECCCCCCEEEEECCCHH
VMDSMLRDVVAHGTARRAVALKRADAAGKTGTSNGSKDVWFAGYSSGVVSVVWMGYDAPR
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCHH
SLGRATGSSAALPVWVDYMKTAVDGRGAIERTPPADVALVDGDFVYAEYANGDKCAPSLP
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCC
PFVRSPFACGAARGAPADGGAPASAAGAAGAPTPAAVDAAERARVLELFRTED
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]