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The map label for this gene is amtB [C]

Identifier: 75909165

GI number: 75909165

Start: 3662100

End: 3663656

Strand: Direct

Name: amtB [C]

Synonym: Ava_2955

Alternate gene names: 75909165

Gene position: 3662100-3663656 (Clockwise)

Preceding gene: 75909164

Following gene: 75909166

Centisome position: 57.53

GC content: 45.09

Gene sequence:

>1557_bases
ATGTACCAACAGAAATCAAGAACAAGAAATAGGCGCTTTTCTACCAGAAATTACGCTAAAAGTAGACAATCTAACTCGCA
AATCCAGATATTTAATCTAGTCAAAAAACTCTCTCCCAGTTGGCAAGCTTGTATACCTCTGGCTTGTTTGATTGTTTTAG
GTTGGAGTTATGTAGCAGTTGCTCAAGCTCCAGCAGCAGGGCCGACAACAGCAGAACTAAAAGTTGCGCTTGACACCCTA
TGGGTAGCGATCGCTGCGTTCTTAGTATTCTTTATGAATGCTGGTTTTGGTATGTTAGAAACCGGCTTCTGTCGTCAGAA
AAATGCTGTTAACGTTCTTGCCAAAAACTTAATCGTCTTCGCCTTGGCAACCGTTGCTTTTTGGGCAATCGGTTTTGGGT
TAATGTTCGGTGATGGTAATGATTTCATTGGCTTCAATGGTTTTTTTCTCTCAGGAGAAGATAACAGCCCGGCAACAGGT
GACGCTTATAAAGGTGTGTTCAGCGCTTTAAGTTGGGCTGGCGTACCTTTAGCCGCAAAATTCTTATTCCAGTTGGTGTT
TGCTGGGACTGCGGCAACAATTGTCTCTGGTGCAGTTGCCGAAAGAATCAAGTTTGTTGACTTCTTGATTTTCAGCCTTT
TACTTGTAGGTATCGCCTACCCAATTACCGGACACTGGATTTGGGGCGCTGGTTGGTTAGCTGATAGAGGTTTTTGGGAC
TTTGCTGGTTCTACAGTGGTTCACTCAGTTGGTGGTTGGGCAGCTTTGATGGGAGCTGCATTTCTGGGTCCCCGTATTGG
TAAATATCAAGATAAGCAAGTTGTGGCTTTACCCGGTCACAACATGAGTATTGCTACCTTGGGTTGCTTGATTCTGTGGT
TGGGTTGGTTTGGTTTCAACCCTGGTTCTGTAATGGCTGCTGACCCCAATGCTATTACTCACATTGCCTTAACAACTAAT
ATGGCTGGTGCTGTCGGTGGTATTGCTGCTACTATTACTGCGTGGTTGTACTTAGGTAAACCAGACCTATCAATGATTAT
TAATGGCATTTTGGCTGGTTTAGTTGGTATTACTGCATCTTGCGCTTACGTCAGCATTCCTAGTTCTGCGATTATTGGTT
TCATTGCCGGAGTCATAGTTGTTTTTGCTGTGACCTTCTTCGACAAACTCGGTATTGATGACCCAGTAGGAGCGACTTCA
GTTCACCTTGTTTGTGGTGTTTGGGGTACTCTGGCAGTTGGTTTGTGGTCGGTTGGGCCTGGTGTTTACTCTTGGTATGG
TGAAGGACTTGGCCCAGCCAAGGGTTTGTTTGCTGGTGGCGGTTTAGGACAGTTGATTACTCAATTCTTAGGTGCTGCCG
CAGTCGGCGGTATGACTGTACTCGTCAGTAGTATTTTCTGGTTAGCACTGAAAGCTACTTTGGGTATTAGAGTCACCAGA
GAAGAAGAATTGGAAGGCTTGGATATCGGCGAACACGGTATGGAAGCCTACAGTGGATTCCTGAAAGAAGCTAGTCCTGG
TGGATTTGCAGAAGGCAAGACATCTGACGGCTATTAA

Upstream 100 bases:

>100_bases
CACACAAGAACTGATGAGAAAGAGTCTAGAAACTAGGAACTCATCACTCATTACTCTCCTTTGCTGTTAAAGATAGTTGG
AAGGTTAGCAATTCGGACTC

Downstream 100 bases:

>100_bases
AGGGGAAGATTACACAATTACCTAATAAATCCTCTATAGTCCATGACCCCGGTTTCTCGTGGAAATCGGGTTTTTTTCAT
CTAGATTCCCATAAGGGACT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAVAQAPAAGPTTAELKVALDTL
WVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVFALATVAFWAIGFGLMFGDGNDFIGFNGFFLSGEDNSPATG
DAYKGVFSALSWAGVPLAAKFLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLADRGFWD
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQVVALPGHNMSIATLGCLILWLGWFGFNPGSVMAADPNAITHIALTTN
MAGAVGGIAATITAWLYLGKPDLSMIINGILAGLVGITASCAYVSIPSSAIIGFIAGVIVVFAVTFFDKLGIDDPVGATS
VHLVCGVWGTLAVGLWSVGPGVYSWYGEGLGPAKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWLALKATLGIRVTR
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY

Sequences:

>Translated_518_residues
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAVAQAPAAGPTTAELKVALDTL
WVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVFALATVAFWAIGFGLMFGDGNDFIGFNGFFLSGEDNSPATG
DAYKGVFSALSWAGVPLAAKFLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLADRGFWD
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQVVALPGHNMSIATLGCLILWLGWFGFNPGSVMAADPNAITHIALTTN
MAGAVGGIAATITAWLYLGKPDLSMIINGILAGLVGITASCAYVSIPSSAIIGFIAGVIVVFAVTFFDKLGIDDPVGATS
VHLVCGVWGTLAVGLWSVGPGVYSWYGEGLGPAKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWLALKATLGIRVTR
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY
>Mature_518_residues
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAVAQAPAAGPTTAELKVALDTL
WVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVFALATVAFWAIGFGLMFGDGNDFIGFNGFFLSGEDNSPATG
DAYKGVFSALSWAGVPLAAKFLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLADRGFWD
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQVVALPGHNMSIATLGCLILWLGWFGFNPGSVMAADPNAITHIALTTN
MAGAVGGIAATITAWLYLGKPDLSMIINGILAGLVGITASCAYVSIPSSAIIGFIAGVIVVFAVTFFDKLGIDDPVGATS
VHLVCGVWGTLAVGLWSVGPGVYSWYGEGLGPAKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWLALKATLGIRVTR
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=441, Percent_Identity=33.5600907029478, Blast_Score=192, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI32564042, Length=438, Percent_Identity=34.9315068493151, Blast_Score=221, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17550182, Length=438, Percent_Identity=35.1598173515982, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI17550180, Length=447, Percent_Identity=33.5570469798658, Blast_Score=203, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI133931100, Length=441, Percent_Identity=30.6122448979592, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324187, Length=448, Percent_Identity=29.6875, Blast_Score=151, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321559, Length=448, Percent_Identity=27.2321428571429, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=28.3372365339578, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078314, Length=436, Percent_Identity=38.7614678899083, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 54490; Mature: 54490

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAV
CCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AQAPAAGPTTAELKVALDTLWVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVF
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHH
ALATVAFWAIGFGLMFGDGNDFIGFNGFFLSGEDNSPATGDAYKGVFSALSWAGVPLAAK
HHHHHHHHHHHHHHEECCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHH
FLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLADRGFWD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCCHH
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQVVALPGHNMSIATLGCLILWLGWFGFN
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
PGSVMAADPNAITHIALTTNMAGAVGGIAATITAWLYLGKPDLSMIINGILAGLVGITAS
CCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
CAYVSIPSSAIIGFIAGVIVVFAVTFFDKLGIDDPVGATSVHLVCGVWGTLAVGLWSVGP
HHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GVYSWYGEGLGPAKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWLALKATLGIRVTR
CHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEC
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYQQKSRTRNRRFSTRNYAKSRQSNSQIQIFNLVKKLSPSWQACIPLACLIVLGWSYVAV
CCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AQAPAAGPTTAELKVALDTLWVAIAAFLVFFMNAGFGMLETGFCRQKNAVNVLAKNLIVF
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHH
ALATVAFWAIGFGLMFGDGNDFIGFNGFFLSGEDNSPATGDAYKGVFSALSWAGVPLAAK
HHHHHHHHHHHHHHEECCCCCEEEECCEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHH
FLFQLVFAGTAATIVSGAVAERIKFVDFLIFSLLLVGIAYPITGHWIWGAGWLADRGFWD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHCCCCCHH
FAGSTVVHSVGGWAALMGAAFLGPRIGKYQDKQVVALPGHNMSIATLGCLILWLGWFGFN
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
PGSVMAADPNAITHIALTTNMAGAVGGIAATITAWLYLGKPDLSMIINGILAGLVGITAS
CCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
CAYVSIPSSAIIGFIAGVIVVFAVTFFDKLGIDDPVGATSVHLVCGVWGTLAVGLWSVGP
HHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GVYSWYGEGLGPAKGLFAGGGLGQLITQFLGAAAVGGMTVLVSSIFWLALKATLGIRVTR
CHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEC
EEELEGLDIGEHGMEAYSGFLKEASPGGFAEGKTSDGY
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]